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 stonesun
Space Traveler
 
Registered: 05/22/09
Posts: 4,622
Loc: 64º08'00"N 21º56'00"W
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 Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)  3
#15935137 - 03/11/12 10:57 PM (1 year, 2 months ago) |
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Panaeolus bisporus from Colville, Eastern WA.
Original specimen collected by Frizzie (Shroomery) aka Subbalteatus (Mycotopia).
Thank you very much for the print my friend!
Some photos of the collected fruits/habitat:
  
  
The Mushroom Observers Page for the find,
and Frizzie's thread on the subject.
This is my second time around cultivating this species,
the first one was from San Diego, CA from lipas' collection.
You can find the thread here: Panaeolus (Copelandia) bisporus Indoor Cultivation.
As far as I know, this is the only confirmed find in the US other than California.
It appears to be "adventitious and apparently more tolerant of temperate climates" according
to Workman. It has been IDd by him as well, here's the microscopy:
 
I've received the wild print on a microscope slide:
  
I went with the same route I did with the CA bisporus.
Swiped the spores on a few MEA plates.
I've had a little trouble with bacterial contamination in the beginning,
but I've made up some antibiotic agar with gentamicin sulfate and finally I've got
clean growth.
Sterilized rye berries spawn were inoculated with an agar wedge.
It colonized rather fast, in just a week.
Spawned it to a pasteurized substrate mix consisting of horse manure/coir/vermiculite/gypsum (50/30/15/5)
in a small plastic shoebox container.
The mycelium ripped through the substrate again in just a week.
Cased with a thin (1/4") pasteurized 50/50+ casing layer,
and introduce to fruiting conditions.
Pins appeared in 12 days.
They are fatties, as my humidity lacks a little bit but hey, I've got fruits
Clean spawn and the substrate before and after casing:
  
  
First pins:
  
More fruits:
   
  
  
   
  
  
  
   
  
  
  
  
I've got another tub in fruiting, it's got pins forming on it right now:
  
Couple of caps printing and some fruits will be harvested tomorrow.
Will update this thread daily (hopefully...)
Thanks for following!
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Beefy1
GONE
Registered: 05/02/09
Posts: 3,573
Loc: around
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 Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: stonesun]
#15935253 - 03/11/12 11:18 PM (1 year, 2 months ago) |
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Wow.
You post some of the best grow logs I've seen.
Do these die out after just one flush?
The microscopy pictures are great, I believe you have one picture mislabeled though.
Spores at 1000x look the exact same size as 400x
Keep the good stuff coming.
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eLShaMukO
from couch potato to sex tornado
Registered: 04/28/10
Posts: 1,567
Loc: far away
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: Beefy1]
#15935978 - 03/12/12 02:32 AM (1 year, 2 months ago) |
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 husmmoor
moshi moshi
Registered: 04/17/11
Posts: 501
Last seen: 33 minutes, 8 seconds
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: stonesun]
#15936486 - 03/12/12 08:22 AM (1 year, 2 months ago) |
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BTW, very beautiful caps!
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SomeGuy
I feel better now :)
Registered: 04/18/10
Posts: 7,496
Loc: 
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: husmmoor]
#15936538 - 03/12/12 09:07 AM (1 year, 2 months ago) |
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thanks for this  Now I'm gonna have to look a lot harder at the paneaolina foenesecii I find.
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truskool
WTF?? FTW!!
Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: SomeGuy]
#15936668 - 03/12/12 10:08 AM (1 year, 2 months ago) |
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Another amazing grow 
So when are you gonna try some liberty caps?
--------------------
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 Roll it  While I troll it. 
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Wing
The Eye Tyrant
Registered: 04/26/11
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: truskool]
#15936681 - 03/12/12 10:16 AM (1 year, 2 months ago) |
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Yet again stonesun shares some awesome pics of an awesome grow!
TY SS
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My Grow Logs
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stonesun
Space Traveler
Registered: 05/22/09
Posts: 4,622
Loc: 64º08'00"N 21º56'00"W
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: truskool]
#15936861 - 03/12/12 11:28 AM (1 year, 2 months ago) |
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Quote:
Beefy1 said:
The microscopy pictures are great, I believe you have one picture mislabeled though.
Spores at 1000x look the exact same size as 400x
You're right, fixed it. Thanks!
Quote:
truskool said:
So when are you gonna try some liberty caps?
I already have, and failed
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Frizzie
Muskiebanger
Registered: 12/03/11
Posts: 298
Loc: Coral Castle
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: stonesun]
#15937130 - 03/12/12 12:50 PM (1 year, 2 months ago) |
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Well StoneSun.. I just wanted to say thank for that wonderful
presentation. That is an incredible grow!! I suppose this
settles the question as to what they are... For some reason
because they were never really looked at on a larger scale
I had doubts as to if they were true Bisporus or not.
When I picked them, as you might see in the link, I had thought
they were Copelandia Cyanescens. I had been pursuing a rare find
that Some friends and I had discovered over fifteen years earlier
in Spokane that I had thought to be Copelandia Cyanescens based on
photographs and endless searches on the internet.
After collecting a good set of Prints of these. I sent a Print to
WarriorSoul from Topia who was the first to suggest Bisporus. And The
Gill and print I sent to Workman took over a month (it seemed to get
to him) So Even though I sent first To Workman, Warrior was the first
to suggest Bisporus from the double Spore. Later when Workman Id'd them
He noted the forked basidium.
It took a while to find someone to Id them completely and I'm glad You
decided to take a look StoneSun!! Never really believed anyone would
actually grow them out to be honest. Had kind of given up on the idea
that we would ever see that.
Looks like it took some Skill! and those are some real fatties for
sure, The blackened caps are similar looking ot lipas collection but
these have much fatter stems.
It was really an amazing summer that I had found these. Not only was
I able to find the Colville Specimen, but found a mamoth Cinctulus
fruiting and even tracked down one or two specimens from Spokane on the
original lawn that started this whole mad search. Excited to see what
those are.
Edited by Frizzie (03/13/12 12:33 PM)
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swampyAppleseed
Friendliest kid you ever met
Registered: 06/23/11
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: Frizzie]
#15937154 - 03/12/12 12:57 PM (1 year, 2 months ago) |
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--------------------
A little nonsense now and then, is relished by the wisest men
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MrGumball
Cubed
Registered: 05/04/10
Posts: 64
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: swampyAppleseed]
#15937166 - 03/12/12 01:01 PM (1 year, 2 months ago) |
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Beautiful.
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Ran-D
has the razh
Registered: 12/19/10
Posts: 4,804
Loc: California Republic
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: Beefy1]
#15937197 - 03/12/12 01:10 PM (1 year, 2 months ago) |
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Quote:
Beefy1 said:
Wow.
You post some of the best grow logs I've seen.
This. You continue to amaze me
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Javadog
Continuing along
Registered: 05/03/10
Posts: 7,292
Loc: USA
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: Ran-D]
#15937357 - 03/12/12 01:53 PM (1 year, 2 months ago) |
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Fun grow SS!!!
--------------------
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Myco-tek.org
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NeoSporen
Antibiotic cream
Registered: 09/05/09
Posts: 3,957
Loc: Washington State
Last seen: 13 days, 10 hours
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: Javadog]
#15937376 - 03/12/12 01:57 PM (1 year, 2 months ago) |
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 Beautiful as always SS.
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Grande Cheeks 
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Frizzie
Muskiebanger
Registered: 12/03/11
Posts: 298
Loc: Coral Castle
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: NeoSporen]
#15939648 - 03/12/12 10:24 PM (1 year, 2 months ago) |
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So in your harvest did you get any good blueing shots?
Looks like the pictures so far are minus any heavy blueing..
But they still are very beautiful
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What is this place The Truman Show!...
  
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stonesun
Space Traveler
Registered: 05/22/09
Posts: 4,622
Loc: 64º08'00"N 21º56'00"W
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: Frizzie]
#15940237 - 03/13/12 12:34 AM (1 year, 2 months ago) |
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Yeah, I've got some harvest pictures from
a few hours ago.
I'll upload them in a few...
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Frizzie
Muskiebanger
Registered: 12/03/11
Posts: 298
Loc: Coral Castle
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: stonesun]
#15941617 - 03/13/12 12:49 PM (1 year, 2 months ago) |
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 OOOOoooo we cant WAIT!!!!
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What is this place The Truman Show!...
Edited by Frizzie (03/13/12 12:50 PM)
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stonesun
Space Traveler
Registered: 05/22/09
Posts: 4,622
Loc: 64º08'00"N 21º56'00"W
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: Frizzie]
#15941627 - 03/13/12 12:54 PM (1 year, 2 months ago) |
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I didn't get a chance Frizzie, sorry.
I'll do it tonight when I get home, I promise.
And don't worry, plenty of bluing on 'em
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Frizzie
Muskiebanger
Registered: 12/03/11
Posts: 298
Loc: Coral Castle
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: stonesun]
#15944401 - 03/14/12 01:07 AM (1 year, 2 months ago) |
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Quote:
stonesun said:
I didn't get a chance Frizzie, sorry.
I'll do it tonight when I get home, I promise.
And don't worry, plenty of bluing on 'em
  
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What is this place The Truman Show!...
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 Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 3,299
Loc: Washington, USA
Last seen: 5 minutes, 47 seconds
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) [Re: stonesun]
#15946098 - 03/14/12 02:40 PM (1 year, 2 months ago) |
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I'd like to see the gill metuloids to confirm if that is possible. Great work!
--------------------
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