Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection) - Mushroom Cultivation

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stonesun
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Registered: 05/22/09
Posts: 4,622
Loc: 64º08'00"N 21º56'00"W

Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

3
#15935137 - 03/11/12 10:57 PM (1 year, 2 months ago)

Panaeolus bisporus from Colville, Eastern WA.
Original specimen collected by Frizzie (Shroomery) aka Subbalteatus (Mycotopia).
Thank you very much for the print my friend!
Some photos of the collected fruits/habitat:

The Mushroom Observers Page for the find,
and Frizzie's thread on the subject.

This is my second time around cultivating this species,
the first one was from San Diego, CA from lipas' collection.
You can find the thread here: Panaeolus (Copelandia) bisporus Indoor Cultivation.

As far as I know, this is the only confirmed find in the US other than California.
It appears to be "adventitious and apparently more tolerant of temperate climates" according
to Workman. It has been IDd by him as well, here's the microscopy:

I've received the wild print on a microscope slide:

I went with the same route I did with the CA bisporus.
Swiped the spores on a few MEA plates.
I've had a little trouble with bacterial contamination in the beginning,
but I've made up some antibiotic agar with gentamicin sulfate and finally I've got
clean growth.
Sterilized rye berries spawn were inoculated with an agar wedge.
It colonized rather fast, in just a week.
Spawned it to a pasteurized substrate mix consisting of horse manure/coir/vermiculite/gypsum (50/30/15/5)
in a small plastic shoebox container.
The mycelium ripped through the substrate again in just a week.

Cased with a thin (1/4") pasteurized 50/50+ casing layer,
and introduce to fruiting conditions.

Pins appeared in 12 days.
They are fatties, as my humidity lacks a little bit but hey, I've got fruits :shrug:

Clean spawn and the substrate before and after casing:

First pins:

More fruits:

I've got another tub in fruiting, it's got pins forming on it right now:

Couple of caps printing and some fruits will be harvested tomorrow.
Will update this thread daily (hopefully...)
Thanks for following!

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Beefy1
GONE

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Posts: 3,573
Loc: around

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15935253 - 03/11/12 11:18 PM (1 year, 2 months ago)

Wow.

You post some of the best grow logs I've seen.

Do these die out after just one flush?

The microscopy pictures are great, I believe you have one picture mislabeled though.

Spores at 1000x look the exact same size as 400x

Keep the good stuff coming.

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eLShaMukO
from couch potato to sex tornado

Registered: 04/28/10
Posts: 1,568
Loc: far away

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Beefy1]
#15935978 - 03/12/12 02:32 AM (1 year, 2 months ago)

--------------------

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husmmoor
moshi moshi

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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15936486 - 03/12/12 08:22 AM (1 year, 2 months ago)

BTW, very beautiful caps!

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SomeGuy
I feel better now :)

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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: husmmoor]
#15936538 - 03/12/12 09:07 AM (1 year, 2 months ago)

thanks for this :thumbup:

Now I'm gonna have to look a lot harder at the paneaolina foenesecii I find. :bow2:

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truskool
WTF?? FTW!!

Registered: 06/28/09
Posts: 11,194
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: SomeGuy]
#15936668 - 03/12/12 10:08 AM (1 year, 2 months ago)

Another amazing grow :stonesun:

So when are you gonna try some liberty caps?

--------------------
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Wing
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: truskool]
#15936681 - 03/12/12 10:16 AM (1 year, 2 months ago)

Yet again stonesun shares some awesome pics of an awesome grow!

TY SS

--------------------
My Grow Logs

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stonesun
Space Traveler

Registered: 05/22/09
Posts: 4,622
Loc: 64º08'00"N 21º56'00"W

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: truskool]
#15936861 - 03/12/12 11:28 AM (1 year, 2 months ago)

Quote:

Beefy1 said:
The microscopy pictures are great, I believe you have one picture mislabeled though.
Spores at 1000x look the exact same size as 400x

You're right, fixed it. Thanks!

Quote:

truskool said:
So when are you gonna try some liberty caps?

I already have, and failed :shrug:

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Frizzie
Muskiebanger

Registered: 12/03/11
Posts: 298
Loc: Coral Castle

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15937130 - 03/12/12 12:50 PM (1 year, 2 months ago)

Well StoneSun.. I just wanted to say thank for that wonderful
presentation. That is an incredible grow!! I suppose this
settles the question as to what they are... For some reason
because they were never really looked at on a larger scale
I had doubts as to if they were true Bisporus or not.

When I picked them, as you might see in the link, I had thought
they were Copelandia Cyanescens. I had been pursuing a rare find
that Some friends and I had discovered over fifteen years earlier
in Spokane that I had thought to be Copelandia Cyanescens based on
photographs and endless searches on the internet.

After collecting a good set of Prints of these. I sent a Print to
WarriorSoul from Topia who was the first to suggest Bisporus. And The
Gill and print I sent to Workman took over a month (it seemed to get
to him) So Even though I sent first To Workman, Warrior was the first
to suggest Bisporus from the double Spore. Later when Workman Id'd them
He noted the forked basidium.

It took a while to find someone to Id them completely and I'm glad You
decided to take a look StoneSun!! Never really believed anyone would
actually grow them out to be honest. Had kind of given up on the idea
that we would ever see that.

Looks like it took some Skill! and those are some real fatties for
sure, The blackened caps are similar looking ot lipas collection but
these have much fatter stems.

It was really an amazing summer that I had found these. Not only was
I able to find the Colville Specimen, but found a mamoth Cinctulus
fruiting and even tracked down one or two specimens from Spokane on the
original lawn that started this whole mad search. Excited to see what
those are.

Edited by Frizzie (03/13/12 12:33 PM)

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swampyAppleseed
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]
#15937154 - 03/12/12 12:57 PM (1 year, 2 months ago)

--------------------
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MrGumball
Cubed

Registered: 05/04/10
Posts: 64

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: swampyAppleseed]
#15937166 - 03/12/12 01:01 PM (1 year, 2 months ago)

Beautiful.

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Ran-D
has the razh

Registered: 12/19/10
Posts: 4,828
Loc: California Republic

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Beefy1]
#15937197 - 03/12/12 01:10 PM (1 year, 2 months ago)

Quote:

Beefy1 said:
Wow.

You post some of the best grow logs I've seen.

This. You continue to amaze me :thumbup:

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Javadog
Continuing along

Registered: 05/03/10
Posts: 7,295
Loc: USA

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Ran-D]
#15937357 - 03/12/12 01:53 PM (1 year, 2 months ago)

Fun grow SS!!!

:fuckinawesome:

--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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NeoSporen
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Javadog]
#15937376 - 03/12/12 01:57 PM (1 year, 2 months ago)

Beautiful as always SS.

--------------------
Grande Cheeks

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Frizzie
Muskiebanger

Registered: 12/03/11
Posts: 298
Loc: Coral Castle

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: NeoSporen]
#15939648 - 03/12/12 10:24 PM (1 year, 2 months ago)

So in your harvest did you get any good blueing shots?
Looks like the pictures so far are minus any heavy blueing..
But they still are very beautiful

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stonesun
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Registered: 05/22/09
Posts: 4,622
Loc: 64º08'00"N 21º56'00"W

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]
#15940237 - 03/13/12 12:34 AM (1 year, 2 months ago)

Yeah, I've got some harvest pictures from
a few hours ago.
I'll upload them in a few...

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Frizzie
Muskiebanger

Registered: 12/03/11
Posts: 298
Loc: Coral Castle

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15941617 - 03/13/12 12:49 PM (1 year, 2 months ago)

OOOOoooo we cant WAIT!!!!

--------------------
What is this place The Truman Show!...

Edited by Frizzie (03/13/12 12:50 PM)

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stonesun
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Registered: 05/22/09
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Loc: 64º08'00"N 21º56'00"W

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]
#15941627 - 03/13/12 12:54 PM (1 year, 2 months ago)

I didn't get a chance Frizzie, sorry.
I'll do it tonight when I get home, I promise.
And don't worry, plenty of bluing on 'em :yesnod:

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Frizzie
Muskiebanger

Registered: 12/03/11
Posts: 298
Loc: Coral Castle

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15944401 - 03/14/12 01:07 AM (1 year, 2 months ago)

Quote:

stonesun said:
I didn't get a chance Frizzie, sorry.
I'll do it tonight when I get home, I promise.
And don't worry, plenty of bluing on 'em

--------------------
What is this place The Truman Show!...

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Workman
Psilocybe Microscopist

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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15946098 - 03/14/12 02:40 PM (1 year, 2 months ago)

I'd like to see the gill metuloids to confirm if that is possible. Great work!

--------------------
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stonesun
Space Traveler

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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]

1
#15948770 - 03/15/12 12:12 AM (1 year, 2 months ago)

OK, here we are with some harvest pictures!

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Javadog
Continuing along

Registered: 05/03/10
Posts: 7,295
Loc: USA

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15948812 - 03/15/12 12:20 AM (1 year, 2 months ago)

Nice dark prints. Good work!

JD

--------------------
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Myco-tek.org

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Frizzie
Muskiebanger

Registered: 12/03/11
Posts: 298
Loc: Coral Castle

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Javadog]
#15950895 - 03/15/12 03:16 PM (1 year, 2 months ago)

Quote:

Workman said:
I'd like to see the gill metuloids to confirm if that is possible. Great work!

To see if what is possible??? Kind of missed that sorry.

And StoneSun, you are one of my heroes! This is such an unexpected
and wonderful end to an amazing discovery.. I wish I could have
seen them in person (grow that is).

Is there any further insight as to if they are the exact species
of Bisporus collected in CA or are they different in any way?
Notice those black caps are the same or similar at least.

The black staining is horrendous. Not even close to blue hardly.
Doesn't that mean large amount of baeosistine? They look scary.
Love to hear a trip report someday if anyones ever got the guts.
I dont.

Anyway, thanks for taking the time to post your work. You rock
:sunny:

Frizz

--------------------
What is this place The Truman Show!...

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Javadog
Continuing along

Registered: 05/03/10
Posts: 7,295
Loc: USA

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]
#15950987 - 03/15/12 03:38 PM (1 year, 2 months ago)

"Metuloids are thick-walled cystidia with an apex having any of several distinct shapes."

From: http://en.wikipedia.org/wiki/Cystidium

Cystidia are used to identify species....kinda like using the pattern
of coloration to distinguish whales. (well, kinda ;0)

Take care,

JD

--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Workman
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]
#15951262 - 03/15/12 04:36 PM (1 year, 2 months ago)

Quote:

Frizzie said:
Quote:

Workman said:
I'd like to see the gill metuloids to confirm if that is possible. Great work!

To see if what is possible??? Kind of missed that sorry.

I was asking if it was possible for stonesun to post a micrograph of a gill surface to show the metuloids. I just want to make sure the species is correct since the cultivated specimens look somewhat similar to P. olivaceus (also active).

The dark coloration is just the natural cap coloration (ignoring the dark spore deposits on some of the capes) and not related to potency or bluing. The California collection is also fairly dark in color.

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stonesun
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Workman]
#15956676 - 03/16/12 08:29 PM (1 year, 2 months ago)

Quote:

Workman said:
I'd like to see the gill metuloids to confirm if that is possible. Great work!

Here they are.
Abudant metuloid pleurocystidia, sizes between 40-50µm.
Thick walled, yellowish color (mounted in water) with dark green towards the apex.

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Workman
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15963055 - 03/18/12 01:39 PM (1 year, 2 months ago)

Yeah baby!!!

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Frizzie
Muskiebanger

Registered: 12/03/11
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Loc: Coral Castle

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Workman]
#15963810 - 03/18/12 05:38 PM (1 year, 2 months ago)

Quote:

stonesun said:
Quote:

Workman said:
I'd like to see the gill metuloids to confirm if that is possible. Great work!

Here they are.
Abundant metuloid pleurocystidia, sizes between 40-50µm.
Thick walled, yellowish color (mounted in water) with dark green towards the apex.

Quote:

Workman said:
Yeah baby!!!

So What do those Metuloid pleurocystidia tell ya Workman? I'm guessing that they confirm copelandia because of the green on the apex?

Or do they resemble olivaceus?

Anything strange or unusual here or are they run of the mill Bisporus?

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What is this place The Truman Show!...

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Workman
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]
#15966576 - 03/19/12 11:17 AM (1 year, 2 months ago)

It just confirms Copelandia and discounts P. olivaceus. The green metuloid apex is normal for all Copelandia when mounted in water so that isn't significant. It's probably not different from the California collection, but more careful comparisons would be required.

--------------------
Research funded by the patrons of
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Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification

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stonesun
Space Traveler

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Posts: 4,622
Loc: 64º08'00"N 21º56'00"W

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]

1
#15992102 - 03/25/12 01:38 AM (1 year, 1 month ago)

Here's a second and third flush update.
The second flush was still fruited indoors in
my growroom.

As you can see a little nicer fruits,
but still a bit short and fat for Panaeolus, often with flattened stipes.

The weather's been pretty nice here, in the low 80s,
with high humidity, so I've decided to let the third flush fruit outside.
I've put the box in my screened lanai by the pool in a bright place
with indirect sunlight.
I've meant to check it yesterday, or even the day before, but I've been busy as hell,
so didn't get a chance.
Here's what I got when I finally get to it today:

Most of these fruits are 150mm tall (~6") and some caps are around 40mm diameter. :thumbup:

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amungus
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: stonesun]
#15992201 - 03/25/12 02:13 AM (1 year, 1 month ago)

Looking good man,once again your post amaze and inspire me.
Thanks for the awesome pictures to drool over.

--------------------
:penis:

"They called me mad, and I called them mad, and damn them, they outvoted me." - Nathaniel Lee

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Frizzie
Muskiebanger

Registered: 12/03/11
Posts: 298
Loc: Coral Castle

Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: amungus]
#15992498 - 03/25/12 03:48 AM (1 year, 1 month ago)

Holy Shit StoneSun... :aweyeah:

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What is this place The Truman Show!...

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truskool
WTF?? FTW!!

Registered: 06/28/09
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]
#15992541 - 03/25/12 04:07 AM (1 year, 1 month ago)

They exploded outdoors eh? Very nice as always :stonesun:

--------------------
Ask AMU for the best mycology advice out there

Roll it

While I troll it. :maximumtrolling:

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Frizzie
Muskiebanger

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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: truskool]
#15992569 - 03/25/12 04:27 AM (1 year, 1 month ago)

Yeah I'd like to Nom this for March..
That and about ten others..

StoneSun I'm absolutely Speechless and dumbfounded that those
things are so Hardy and beautiful! You're such a lucky man.
Thanks so much for sharing with all of us! You obviously put
a lot of sweat in to it. Keep on Keepin On!

Ps. I dare ya to eat just one of those meaty buggers!
lol... jkn, I would never recommend that to anyone actually.
They've got to be more potent than azures. it would be neat
to see some studies done on them. You should send a random
sample to the Pan Expert of the day. Who is it Gerhardt?
I cant remember. But that would be neat to see the compounds
broken down and compared to the other recent as well as more
established varieties.

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What is this place The Truman Show!...

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swampyAppleseed
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Re: Panaeolus (Copelandia) bisporus/Colville, WA (Frizzie/Subbalteatus Collection)

[Re: Frizzie]
#15993875 - 03/25/12 02:46 PM (1 year, 1 month ago)

SO beautiful! again and again! :congrats:

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