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 Bluemookie
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 Does this mycellium look normal?
#15785182 - 02/09/12 05:40 PM (3 months, 17 days ago) |
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Some spots look whiter than others. My last mono of Cambodian came up with trich, so I'm overly anxious about my new PESA jars. They were inoculated on 1/17 and the first shake was on 2/1. They were inoculated with LC. What are your thoughts?
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 Willy Wonka
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15785184 - 02/09/12 05:41 PM (3 months, 17 days ago) |
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Yup looks great.
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"We do not possess imagination enough to sense what we are missing." -Jean Toomer
  
Edited by Willy Wonka (02/09/12 07:36 PM)
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k00laid
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Re: Does this mycellium look normal? [Re: Willy Wonka]
#15785208 - 02/09/12 05:48 PM (3 months, 17 days ago) |
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not to me
edit: but time will tell all
Edited by k00laid (02/09/12 05:48 PM)
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Swwert
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Re: Does this mycellium look normal? [Re: k00laid]
#15785437 - 02/09/12 06:45 PM (3 months, 17 days ago) |
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Quote:
k00laid said:
not to me
edit: but time will tell all
Why not? (serious question)
I think they look perfectly fine
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k00laid
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Re: Does this mycellium look normal? [Re: Swwert]
#15785636 - 02/09/12 07:27 PM (3 months, 17 days ago) |
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ehh i just wanna see it in 4 days
, temperature op?
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thedoc8
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15785647 - 02/09/12 07:29 PM (3 months, 17 days ago) |
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Quote:
Bluemookie said:
Some spots look whiter than others. My last mono of Cambodian came up with trich, so I'm overly anxious about my new PESA jars. They were inoculated on 1/17 and the first shake w
on 2/1. They were inoculated with LC. What are your thoughts?
Looks really good to me. But in that length of time I would expected them to be farther along.
Edited by thedoc8 (02/09/12 07:36 PM)
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PureHash
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Re: Does this mycellium look normal? [Re: k00laid]
#15785651 - 02/09/12 07:29 PM (3 months, 17 days ago) |
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the grains look a little dry, and the growth looks a little weak. but depending when you last shook the jars could say why they look weak.
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Willy Wonka
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Re: Does this mycellium look normal? [Re: thedoc8]
#15785711 - 02/09/12 07:41 PM (3 months, 17 days ago) |
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Quote:
thedoc8 said:
Looks really good to me. But in that length of time I would expected them to be farther along.
But keep in mind the OP said this picture was taken after a shake. Considering the myc has to regain it's foot hold and continue its growth I think it looks good.
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"We do not possess imagination enough to sense what we are missing." -Jean Toomer
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thedoc8
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Re: Does this mycellium look normal? [Re: Willy Wonka]
#15785735 - 02/09/12 07:47 PM (3 months, 17 days ago) |
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Quote:
Willy Wonka said:
Quote:
thedoc8 said:
Looks really good to me. But in that length of time I would expected them to be farther along.
But keep in mind the OP said this picture was taken after a shake. Considering the myc has to regain it's foot hold and continue its growth I think it looks good.
Did not see when he took shot in his post.
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Bluemookie
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Re: Does this mycellium look normal? [Re: k00laid]
#15785736 - 02/09/12 07:47 PM (3 months, 17 days ago) |
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Quote:
k00laid said:
ehh i just wanna see it in 4 days
, temperature op?
It should be 69-70 degrees. I will post more pictures next week. I just thought I would get a general consensus. The last couple runs took a long time (6 weeks) and I was told it's because you don't use Whatman's lids on grain jars, so I made my own lids with polyfill. The next batch will have SFD, which I had to shop around for a decent price.
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Dr. No Clue
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15786082 - 02/09/12 09:17 PM (3 months, 17 days ago) |
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Grain looks dry, mycelium is healthy just growing kind of thin make sure you give it time to consolidate when it finishes up.
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Dupree
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15786122 - 02/09/12 09:27 PM (3 months, 17 days ago) |
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Quote:
Bluemookie said:
Quote:
k00laid said:
ehh i just wanna see it in 4 days
, temperature op?
It should be 69-70 degrees. I will post more pictures next week. I just thought I would get a general consensus. The last couple runs took a long time (6 weeks) and I was told it's because you don't use Whatman's lids on grain jars, so I made my own lids with polyfill. The next batch will have SFD, which I had to shop around for a decent price.
the temp should be a lil higher than that for colonization, shouldnt it? around 75-80?
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Bluemookie
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Re: Does this mycellium look normal? [Re: Dupree]
#15787338 - 02/10/12 04:55 AM (3 months, 17 days ago) |
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Quote:
Dupree said:
Quote:
Bluemookie said:
Quote:
k00laid said:
ehh i just wanna see it in 4 days
, temperature op?
It should be 69-70 degrees. I will post more pictures next week. I just thought I would get a general consensus. The last couple runs took a long time (6 weeks) and I was told it's because you don't use Whatman's lids on grain jars, so I made my own lids with polyfill. The next batch will have SFD, which I had to shop around for a decent price.
the temp should be a lil higher than that for colonization, shouldnt it? around 75-80?
For rye grain? Nope. Room temperature is what's called for.
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Ill-bird
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Re: Does this mycellium look normal? [Re: Dr. No Clue]
#15787382 - 02/10/12 05:29 AM (3 months, 17 days ago) |
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Quote:
Dr. No Clue said:
Grain looks dry, mycelium is healthy just growing kind of thin make sure you give it time to consolidate when it finishes up.
Dude why would you tell somebody to let grain spawn consolidate? Once it reaches 100% you spawn to bulk while mycelia is in a vigorous growing state. If it's let set to consolidate then it's growth slows down in preparation for fruiting mode.
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Dr. No Clue
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Re: Does this mycellium look normal? [Re: Ill-bird]
#15787923 - 02/10/12 09:06 AM (3 months, 17 days ago) |
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you need to let it consolidate to ensure it gets a good hold of the grain or when you spawn to bulk grains that are not fully colonized will come in contact with air and possibly get contaminated, if you have a jar that is solid white theres no need to let it sit more than 1 day if you have a jar that is light but fully colonized you just give it maybe 3 days or so to make sure it takes a good hold of the grain. either ways mycelium will not slow down in preparation for fruiting mode unless you leave it for quite a long time (when food and water runs out it will try to fruit) or expose it to fruiting conditions, not everyone can immediately spawn to bulk as soon as the jar is 100% it wont hurt unless you leave it longer than a week and if you need to all you have to do is place it in the fridge.
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k00laid
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Re: Does this mycellium look normal? [Re: Dr. No Clue]
#15788212 - 02/10/12 10:26 AM (3 months, 17 days ago) |
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Quote:
Dr. No Clue said:
you need to let it consolidate to ensure it gets a good hold of the grain
nope.
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Ill-bird
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Re: Does this mycellium look normal? [Re: Dr. No Clue]
#15790466 - 02/10/12 09:14 PM (3 months, 16 days ago) |
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Consolidation is after colonization. When mycelia are consolidating they are digesting the nutritional substance they just colonized. 
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Dr. No Clue
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Re: Does this mycellium look normal? [Re: Ill-bird]
#15790511 - 02/10/12 09:31 PM (3 months, 16 days ago) |
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ok, I used the wrong terminology after it looks fully colonized just give it another day or two, use the search function as far as I have read most trusted cultivators do so.
and after googling it I found this.
Colonization refers the process of mushroom mycelium consolidating its hold on a substrate by extending new strands of mycelium into nutritious material.
like I said don't leave it for two weeks, its a good practice to leave it another day or two to make sure its got a good hold on the grain and less susceptible to contams.
Edited by Dr. No Clue (02/10/12 09:39 PM)
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k00laid
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Re: Does this mycellium look normal? [Re: Dr. No Clue]
#15792068 - 02/11/12 09:28 AM (3 months, 16 days ago) |
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Quote:
Dr. No Clue said:
ok, I used the wrong terminology after it looks fully colonized just give it another day or two,
i dont for grains.
i give a week for PF CAKES
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Willy Wonka
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15793691 - 02/11/12 04:16 PM (3 months, 15 days ago) |
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So how are the jars looking now?
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Bluemookie
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Re: Does this mycellium look normal? [Re: Willy Wonka]
#15804217 - 02/13/12 04:30 PM (3 months, 13 days ago) |
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Here's what they look like as of this evening. To me, it looks like a couple jars should be shook up again, but that's me. This is side one of four for all the jars. No, I don't plan to upload pictures of all four sides.
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Willy Wonka
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15805332 - 02/13/12 07:28 PM (3 months, 13 days ago) |
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I would wait to shake again. That might be part of the problem. How often are you shaking the jars? If you shake to often it might cause the myc to stall. Shaking the stuff shocks the myc and it has to recover each time it is shook before it will continue to grow again. Give it some time to recover.
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Bluemookie
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Re: Does this mycellium look normal? [Re: Willy Wonka]
#15807047 - 02/14/12 05:13 AM (3 months, 13 days ago) |
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I only shook them once, but it might have been too soon for a couple jars. Either that or I didn't shake them well enough.
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hellokitty
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15807051 - 02/14/12 05:15 AM (3 months, 13 days ago) |
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its fine
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k00laid
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15807222 - 02/14/12 06:47 AM (3 months, 13 days ago) |
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Quote:
Bluemookie said:
looks fucked to me 
its like almost grey and powdery.
there's only one spot on your jar that looks as white and thick as this.
idk man.
i;d keep waiting but it looks like mold in there.
or maybe extremely dry grains.
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kdmmontana
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Re: Does this mycellium look normal? [Re: k00laid]
#15807645 - 02/14/12 09:37 AM (3 months, 13 days ago) |
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Fucked IMO
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Bluemookie
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Re: Does this mycellium look normal? [Re: kdmmontana]
#15841345 - 02/21/12 04:58 AM (3 months, 6 days ago) |
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Okay, here's what it looks like this week. I'm mostly concerned about jars 1 and 2.
      
I can't help but think these should get another shake (the first 2) and take 2 weeks to recover. What do you guys think? What about the rest of the jars? Am I okay? Do they look normal? The consensus is still far from unanimous.
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hellokitty
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15841364 - 02/21/12 05:10 AM (3 months, 6 days ago) |
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they look fine. shake them if you want; but there is no reason they should take 2 weeks to recover, more like 2 days.
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k00laid
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Re: Does this mycellium look normal? [Re: hellokitty]
#15842220 - 02/21/12 10:25 AM (3 months, 6 days ago) |
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yup
those are all clean
the grains might b a bit too dry to colonize.
but you should b able to spawn those
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Bluemookie
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Re: Does this mycellium look normal? [Re: k00laid]
#15842640 - 02/21/12 12:04 PM (3 months, 6 days ago) |
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So, if I prepare the grains exactly as instructed in the RR video, what do you do when things dry out?
Should I shake the 2 that have uncolonized areas?
Is the only way to determine if these jars are contaminated, just open them when finished and smell them?
Or do I have to mix it with coir and find out if trich grows?
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k00laid
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15842652 - 02/21/12 12:06 PM (3 months, 6 days ago) |
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Quote:
Bluemookie said:
Or do I have to mix it with coir and find out if trich grows?
probably no trich.
but maybe some bacteria or something on the uncolonized parts.
its hard to tell. they are probably fine.
and you should soak longer IMO
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dammdoggs
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Re: Does this mycellium look normal? [Re: PureHash]
#15842909 - 02/21/12 01:16 PM (3 months, 6 days ago) |
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Quote:
PureHash said:
the grains look a little dry, and the growth looks a little weak. but depending when you last shook the jars could say why they look weak.
This
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Bluemookie
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Re: Does this mycellium look normal? [Re: k00laid]
#15842919 - 02/21/12 01:19 PM (3 months, 6 days ago) |
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Quote:
k00laid said:
Quote:
Bluemookie said:
Or do I have to mix it with coir and find out if trich grows?
probably no trich.
but maybe some bacteria or something on the uncolonized parts.
its hard to tell. they are probably fine.
and you should soak longer IMO
Longer than 24 hours? How long would you soak them?
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Waldfried
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15843075 - 02/21/12 02:00 PM (3 months, 5 days ago) |
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So if a grain jar stalled because of dry grains, is it ok to spawn it without 100% colonization?
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k00laid
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15843459 - 02/21/12 03:28 PM (3 months, 5 days ago) |
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you soak em in really hot water right?
and if you dont wanna soak more than 24 hours, then boil them for longer
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k00laid
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Re: Does this mycellium look normal? [Re: Waldfried]
#15843463 - 02/21/12 03:28 PM (3 months, 5 days ago) |
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Quote:
Waldfried said:
So if a grain jar stalled because of dry grains, is it ok to spawn it without 100% colonization?
ive had more contamination problems with spawn not done cuz of dryness
but its also worked some
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hellokitty
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Re: Does this mycellium look normal? [Re: k00laid]
#15843504 - 02/21/12 03:43 PM (3 months, 5 days ago) |
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its actually quite hard to get your grains too dry for colonization, im not saying it doesnt happen but if you have soaked then boiled/simmered for even a short amount of time you should be fine, normally you would see the berries dry on the top if that is the issue, a simple shake once the rest is colonized will solve the issue.
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Bluemookie
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Re: Does this mycellium look normal? [Re: hellokitty]
#15846504 - 02/22/12 07:33 AM (3 months, 5 days ago) |
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Okay, my prep.
Rinse the rye grain under hot water. I usually rinse about 5 times. I then fill the pot with hot water and add about a teaspoon of gypsum and let sit for 24 hours. After the soak, I bring it to a boil for about 10-15 minutes before straining. I tried one to go 30 minutes, but I found a substantial amount of popped kernels. Nowhere near 5%, but still. I then strain and shake in strainer for about 30 minutes. I test a little bit on toilet paper to make sure enough water has evaporated and then I load my jars. I then cover with foil and PC them for 90 minutes. I let the PC cool over night.
The only way it would have dried out is because I drilled a 3/8" hole and filled with poly-fill. Maybe 3/8" is too big. My next jars will be 3/8" with a SFD covering the hole with RTV silicone.
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Bluemookie
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15851103 - 02/23/12 05:21 AM (3 months, 4 days ago) |
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So, what's my play here? Do I spawn them with uncolonized grains? Do I shake them again? Do I just wait it out?
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hellokitty
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15851134 - 02/23/12 05:41 AM (3 months, 4 days ago) |
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never spawn uncolonized grains. if they are not colonizing then shake. % wise how much is not colonized? and yeah i would go with SFD or E-Zfelt(much cheaper and ive never had any issues)
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Mr. Anderson
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Re: Does this mycellium look normal? [Re: hellokitty]
#15851172 - 02/23/12 06:03 AM (3 months, 4 days ago) |
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Inject sterile water then shake
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Bluemookie
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Re: Does this mycellium look normal? [Re: Mr. Anderson]
#15851293 - 02/23/12 06:49 AM (3 months, 4 days ago) |
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Inject sterile water? Really? How much? And how? Do I just boil some water, suck up into a hypo and wait for it to cool, and then flame the needle and inject? Isn't this a bit risky?
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Mr. Anderson
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15851350 - 02/23/12 07:05 AM (3 months, 4 days ago) |
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Not really, I've done it before. If you have big 60 cc syringes you can just suck up boiling water then pc the syringe. That way with 60 you would have more then enough water. Wipe your injection port with alcohol before sticking the syringe in but if u pc the syringe you don't have to do all that other shit.
I haven't had grains dry out like that in a long long time but when I started with rye I had it happen once, you'd probably use like 10-20 ml. Just kinda gotta guess and have a feel for things.
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Dr. No Clue
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Re: Does this mycellium look normal? [Re: Mr. Anderson]
#15851537 - 02/23/12 07:59 AM (3 months, 4 days ago) |
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Mark your jars with a marker around the mycelium and check back tomorrow for growth, if you see slow growth even if slow the wait it out, if you see no growth then they stalled and you should look for signs of contamination or post more pics so we can help u out. I had a jar stall due to dryness and I injected with a little sterilized water, it finished up in 3 days.
don't just boil your grains, make believe they are rice or pasta or whatever and when you boil make sure they cook. They should be soft and thoroughly cooked taste them squeeze them between your fingers or whatever you want. this made all the difference in the world for me and my jars finish up in 7-10 days since I began doing this.
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Bluemookie
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Re: Does this mycellium look normal? [Re: Dr. No Clue]
#15851563 - 02/23/12 08:07 AM (3 months, 4 days ago) |
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After soaking and boiling, they are soft. Very plump. When I load them into my jars, the water is still evaporating, so the jars look very wet inside. Within a few days, all of that does away. It's like the 3/8" hole is too big. The last batch of rye that I did colonized slow, and I was told it was because I was using Watman lids and those are only for LC, and the there isn't enough GE. So, this time, I used 3/8" holes stuffed with poly, but it looks like the grains are drying out or something.
As for the syringe stuff, all I have is the 10mm syringes that my spores have shipped in. So, you're saying to fill up the syringes (I only have 2 or 3 empty syringes) and PC them? And then wait for it to cool? Really? Um...that's going to take like an hour, and I'll have to do it several times! Don't people just microwave them or something? I'm still not convinced my grains are dry! I followed all the steps, so they should be fine. Maybe there's a contam...
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Dr. No Clue
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15851667 - 02/23/12 08:34 AM (3 months, 4 days ago) |
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start using tyvek, possibly ambient humidity is very low and you have too much moisture escaping through polyfill. I still think its your grain prep, from your description you are loading grains too wet and the fact that within a few days they look completely dry and absorb all the moisture says to me that they where not cooked correctly otherwise you would have condensation all over the top of the jar from the water evaporating off the grains. Dont microwave PC the syringe, it may take longer but in the long run the extra hour of work will save you weeks and the heartache of loosing all your work. You dont really need that much water either, I used 2ccs in my jar and it was enough. only jars that need the water are the first two, having a few uncolonized grains on top is fine, it happens all the time since they are the first to dry out just take them off with a spoon before spawning to bulk.
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Mr. Anderson
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Re: Does this mycellium look normal? [Re: Dr. No Clue]
#15851693 - 02/23/12 08:41 AM (3 months, 4 days ago) |
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You just do your syringes like you'd do to make spore syringes. Mark your jars tho and see if there's still slow growth, if anything you just may need to shake em or just wait it out. You could just inject water and wait to if its just cause its dry .
I boil all my grain for 15 mins after a 24 hour soak(give or take). But like I'm talking a roaring boil heat cranked all the way up. Not a pussy simmer. I barely steam my grains either, I go right into the strainer then just start spooning strait to jars. Works fuckn fine for me.
I just like everything as easy as possible.
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Disclaimer: All posts are completely fictional and or for educational purposes only.
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Bluemookie
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Registered: 08/30/11
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Re: Does this mycellium look normal? [Re: Dr. No Clue]
#15851717 - 02/23/12 08:46 AM (3 months, 4 days ago) |
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Well, for the record, it's just slow, not stalled, IMO. I'll take pictures tonight or this weekend. Maybe if I just wait it out, I won't have to do anything. I don't see what I could have done any differently with the grain prep though, other than use SFD, which I didn't have at the time, but I do now. Next batch will have SFD. I'm preparing 2 grain masters to try G2G for my next mono. So, if there's something else in my grain prep that is suspect, please tell me! Soak for 24 hours. Boil for 15 minutes. Drain for 30 minutes. Load into jars and PC. What am I missing?
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Mr. Anderson
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Re: Does this mycellium look normal? [Re: Bluemookie]
#15851759 - 02/23/12 09:00 AM (3 months, 4 days ago) |
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Yea I skip the 30 min drain part, have forever.
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Disclaimer: All posts are completely fictional and or for educational purposes only.
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Bluemookie
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Re: Does this mycellium look normal? [Re: Mr. Anderson]
#15945371 - 03/14/12 07:18 AM (2 months, 15 days ago) |
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Okay, so I injected sterile water into my 7 jars on 3/5. The pic on the left is from 2/21. I shook the jars shortly after and gave them about 2 weeks to recover, but they never really did. They just looked dry. So, I gave each jar about 5-6 cc's of sterile water and shook again. It's been 9 days since the sterile water and second shake. How do they look? How much longer should I let them stand before spawning to coir?
 
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