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 dr.psilocybe
Doctor
Registered: 01/29/12
Posts: 61
Last seen: 3 days, 19 hours
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 Re: Need help- Possible Contam  [Re: Kizzle]
#15795125 - 02/11/12 08:59 PM (3 months, 15 days ago) |
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Sad news, however, I appreciate your guidance and advice.
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dr.psilocybe
Doctor
Registered: 01/29/12
Posts: 61
Last seen: 3 days, 19 hours
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I believe I may have found one source of the contamination problem. I was trying to incubate in my closet using a space heater with a digital temp. control. For some reason the temperature would always get so high that it would shut down and I'd turn it back on. I started leaving the door of my closet open and regulating it with a combo of my heater and a/c until it said about 78 degrees. It seemed like while I was using this, every day I'd wake up the next morning to find another contamination in one of my jars. Since, I have stopped using the heater and just leaving the door closed. It seems that the jars have started colonizing a lot faster even though it isnt at optimal temperature and I haven't had anymore contaminations thus far (fingers crossed ). I think the heater may have been allowing for contamination. I don't think it's the only answer, but it seems to be a big part of it from my observations.
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aznboy
Toad
Registered: 10/14/11
Posts: 61
Last seen: 13 hours, 33 minutes
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yeah seems like the warmer the temp the more contamination you get.
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Sulfurshelfsean
Defender of Cubes
Registered: 07/29/10
Posts: 358
Last seen: 14 minutes, 11 seconds
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Let it colonize at room temp. 85 is ideal for myc. and contams.
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hellokitty
money or your life...
Registered: 01/01/12
Posts: 305
Last seen: 2 months, 15 days
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break your verm up next time and i dont think you will have any issues, also you should flame sterilize your needle between each jar to avoid cross contamination.
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dr.psilocybe
Doctor
Registered: 01/29/12
Posts: 61
Last seen: 3 days, 19 hours
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Re: Need help- Possible Contam [Re: hellokitty]
#15808175 - 02/14/12 12:22 PM (3 months, 13 days ago) |
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Yea I thing the coarse verm was a factor too. I did sterilize the needle with a flame until red hot each time and used alcohol, although I didn't use a glove box. I will be more careful next time with a glove box, finer verm and lysol
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Sulfurshelfsean
Defender of Cubes
Registered: 07/29/10
Posts: 358
Last seen: 14 minutes, 11 seconds
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Lysol spray=My best friend.
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dr.psilocybe
Doctor
Registered: 01/29/12
Posts: 61
Last seen: 3 days, 19 hours
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I'll keep that in mind!
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