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 Poor Boy
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Registered: 01/16/12
Posts: 3,770
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 injection ports 
#15757243 - 02/03/12 05:36 PM (3 months, 23 days ago) |
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how do you tell if the ports sealed all the way? im now extremely paranoid about the needle possible being too hot and not being sterile enough cause the needle was not red hot.
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Solids and liquids are differentiated, one from the other, by the way the material responds to stress.If the material deforms and stays deformed, springs back when the stress is removed, it's a solid.
If instead the material flows when stressed, rearranges itself in such a way as to remove the stress, it's a liquid. . . .
We're gonna have to adapt if we expect to survive.
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Wise Toad
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Posts: 2,223
Loc: Flipside
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Re: injection ports [Re: Poor Boy]
#15757334 - 02/03/12 05:56 PM (3 months, 23 days ago) |
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RTV right?
Iv never had a problem unless its like the 10th injection through the same port and some have survived longer than that
I don't know what to say about the red hot part, you have an alcohol lamp right? if you dont check my journal and the bottom link in my sig
What gauge are the needles, I have had some lower gauge needles(14/16 gauge) rip up my port
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Poor Boy
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they are from sporeworks. not sure what gauge they were. everyone seems to say the needle needed to be red hot, but i think it should be fine after hanging over the flame for a good 10 seconds.
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Solids and liquids are differentiated, one from the other, by the way the material responds to stress.If the material deforms and stays deformed, springs back when the stress is removed, it's a solid.
If instead the material flows when stressed, rearranges itself in such a way as to remove the stress, it's a liquid. . . .
We're gonna have to adapt if we expect to survive.
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EvilMushroom666
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Registered: 11/18/09
Posts: 9,507
Loc: Canada 
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Re: injection ports [Re: Poor Boy]
#15757683 - 02/03/12 07:05 PM (3 months, 23 days ago) |
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To flame sterilize you need to ensure that the needle is RED HOT. This
will kill all bacteria, molds, contaminate spores etc that are present
both on and inside the needle, and if you are not waiting until your needle
is red hot you are not doing it right.
As for the needle being hot when injecting into ports or RTV, the way to
get around this is to gently allow a few drops of solution to pass into
the needle and it will instantly cool down the needle enough to not burn
whatever you are injecting into.
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Wise Toad
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forgot about that last part, as always good shit Evil; I do open air mostly when injecting jars, would you advise the cooling down protocol for such? Since your here Iv got a few questions for a pro
Its been a while and Im just getting back into it, cant remember if I used to cool the needle outside of the GB or not
Another thing, I always wipe the injection port down with alcohol; is that necessary?
Is there any reason to wear a surgical mask while working in a glove box?
and poor boy, are you talking about those blue stopper injection ports; I used to use those but they wore out quick, I either filled the hollow part with RTV silicone to extend their life or got rid of the lid all together in favor of a plain RTV silicone injection port
carry on gentlemen 
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EvilMushroom666
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Posts: 9,507
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If you are injecting BRF cakes or the like I would not worry about cooling
your needle, only when injecting into jars with self healing ports would
I worry about cooling the needle.
If you are using a glove box likely by the time you have your syringe
back in the box and ready to inoculate the needle will have cooled enough.
Wiping a needle down after flame sterilizing is counter productive, nothing
is more sterile then red hot, and you are opening a vector of contamination.
I wear a surgical mask when doing anything that needs to be sterile, when
you breath you are releasing all sorts of organisms that can contaminate
your work, its never a bad idea to wear a surgical mask when working.
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Kizzle
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Registered: 08/30/11
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Quote:
EvilMushroom666 said:
As for the needle being hot when injecting into ports or RTV, the way to
get around this is to gently allow a few drops of solution to pass into
the needle and it will instantly cool down the needle enough to not burn
whatever you are injecting into.
Have you ever actually burned the port? I use the homemade silicone seals and always push it through while it's still red hot and haven't run into any problems.
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EvilMushroom666
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Re: injection ports [Re: Kizzle]
#15758186 - 02/03/12 09:14 PM (3 months, 23 days ago) |
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I find that when using self healing injection ports and not cooling the
needle it leaves a bigger opening after you pull it back out.
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 deadmeadow
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Registered: 01/31/12
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Last seen: 7 days, 23 hours
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Your injection port is probably fine.. Since you didn't get the needle all that hot and you wiped it, but as far contamination goes all you can do is wait patiently and see unfortunately.
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Wise Toad
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Quote:
EvilMushroom666 said:
If you are using a glove box likely by the time you have your syringe
back in the box and ready to inoculate the needle will have cooled enough.
Wiping a needle down after flame sterilizing is counter productive, nothing
is more sterile then red hot, and you are opening a vector of contamination.
I meant working without a glove box, if your not in one do you still quick cool the needle
and I didnt mean wipe down the needle, wipe down the port that your about to stick the needle through or not?
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Kizzle
Misanthrope
Registered: 08/30/11
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I would wipe the port down first whether or not using a glovebox. Then if you're not using a box I would also wrap an alcohol soaked cotton ball or paper towel around the needle before pulling it out of the hole, and hold it there as you pull the needle out and a few seconds after so it can cover the hole until it reseals.
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Wise Toad
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Posts: 2,223
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Re: injection ports [Re: Kizzle]
#15758875 - 02/04/12 01:46 AM (3 months, 23 days ago) |
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I used to use the whole cotton ball/sponge thing but eventually just found it tedious, even on the way out Iv done nothing and have turned out fine; I have always alcohol wiped the port beforehand though, I dont know if that initial wipe is necessary or not?
I havent had a jar contam on me since my first(horrid) grow, I gotta keep it that way
I think I have always let the needle cool for several seconds without squirting a drop to quick cool when not using a glove box but I just cant remember
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Poor Boy
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should i re order?
--------------------
Solids and liquids are differentiated, one from the other, by the way the material responds to stress.If the material deforms and stays deformed, springs back when the stress is removed, it's a solid.
If instead the material flows when stressed, rearranges itself in such a way as to remove the stress, it's a liquid. . . .
We're gonna have to adapt if we expect to survive.
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Montock
Time = $
Registered: 08/27/09
Posts: 1,082
Last seen: 2 months, 26 days
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Re: injection ports [Re: Poor Boy]
#15759375 - 02/04/12 07:38 AM (3 months, 23 days ago) |
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I use bernzomatic hand held propane torch for my box and hood work, you HAVE to squirt a drop out before using the port, I've yet to find a port, vendor or homemade that I can't destroy if I smoke to much and try to inj while still hawt. Red rtv silicone homeade ones stand the best chance against a red hawt needle tip, but won't last, look trashy . For bags you can inj red hot if you go old school and use duct tape , which works just as well as any port, if not better, and is literally more than 100x cheaper .
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NOT ALL VENDORS ARE EQUAL
The following are vendors I am in good standing with and pledge to all shroomery members, that they can shop with confidence at.
www.sporeworks.com for your genetics
www.mycopath.com for your equipment/raw materials
Other vendors will most likely give you top notch service/product as well. The above are personal recomendations, given via first hand encounters.
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Poor Boy
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Re: injection ports [Re: Montock]
#15759490 - 02/04/12 08:30 AM (3 months, 23 days ago) |
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my biggest concern is not getting the needle red hot...
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Solids and liquids are differentiated, one from the other, by the way the material responds to stress.If the material deforms and stays deformed, springs back when the stress is removed, it's a solid.
If instead the material flows when stressed, rearranges itself in such a way as to remove the stress, it's a liquid. . . .
We're gonna have to adapt if we expect to survive.
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Breadnbutterfly86
Religion limits human potential
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Posts: 840
Loc: Wonderland
Last seen: 16 hours, 8 minutes
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Re: injection ports [Re: Poor Boy]
#15759714 - 02/04/12 09:51 AM (3 months, 23 days ago) |
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no problems here with a flame to the needle for 7, 10 seconds or so and wiping the needle with alcohol afterwards... sterile and cools it. at least in these experiences
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"Don't just do something, stand there!" -Mr. Rabbit-
Breadnbutterfly
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 1,946
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Quote:
Wise Toad said:
I used to use the whole cotton ball/sponge thing but eventually just found it tedious, even on the way out Iv done nothing and have turned out fine; I have always alcohol wiped the port beforehand though, I dont know if that initial wipe is necessary or not?
I havent had a jar contam on me since my first(horrid) grow, I gotta keep it that way
I think I have always let the needle cool for several seconds without squirting a drop to quick cool when not using a glove box but I just cant remember
I've gotten away without it too and with letting the needle cool first for a few seconds. I don't it tedious at all though, usually have the alcohol wipe still sitting there from wiping the surface anyways. I figure in the long run I'm probably saving myself from some contamination but maybe I'm just being anal 
Poor Boy, making an alcohol lamp is extremely simple. It's basically a jar with a hole and a wick (piece of string or cotton) sticking out through the hole. You should consider making one and you won't have any problem getting the needle red hot.
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Wise Toad
Distorted Interpretation
Registered: 06/08/10
Posts: 2,223
Loc: Flipside
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Re: injection ports [Re: Kizzle]
#15760114 - 02/04/12 11:51 AM (3 months, 23 days ago) |
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