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OfflineVibration
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Weird colonization in my monotub - should I remix the substrate?
    #15756966 - 02/03/12 04:34 PM (3 months, 23 days ago)

As you can see, the surface is basically covered with myc, also there's some in the upper half of the substrate, but in the bottom half there is no mycelium growing.

It's a beginning of a day 5 though, but on the bottom (if I look under the tub) there is 0% growth. I can see grains in the coir, but zero growth.

I used 100% colonized mycelium. The substrate moisture content is good. I used 3,5 quarts of water per 1 brick of coir.

I did everything well, I don't understand whats causing this kind of weird growth. Upper half is colonizing, bottom half is not.








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The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.


Edited by Vibration (02/05/12 08:44 AM)


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Offlinezepski
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Re: Weird colonization in my monotub. Why? [Re: Vibration]
    #15757134 - 02/03/12 05:10 PM (3 months, 23 days ago)

I just had a tub of KSSS do that.  It stalled for a few days then i noticed on the bottom a nice big old spot of trich.  I hope your fate is different. 


I say just keep your eye on it since its only been 5 days.  best of luck


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OfflineVibration
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Re: Weird colonization in my monotub. Why? [Re: zepski]
    #15759264 - 02/04/12 06:28 AM (3 months, 23 days ago)

Thanks.


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Offlinesof4r0ckeRs1984
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Re: Weird colonization in my monotub. Why? [Re: Vibration]
    #15759333 - 02/04/12 07:14 AM (3 months, 23 days ago)

I wondered if it was a bit too wet. As one can see the top half of the coir colonizes better then the bottom. The water follows gravitation.
I don't have a good advice on how to dry an oversaturated tub during colonisation... maybe just open one or two holes and when the bottom follows just re-seal it.

If you don't manage to dry it out, it won't colonize all that fine and will consolidate when you try to initiate fruiting. Even if you get no contam, you might get an unsatisfying harvest if you just leave it like that.


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OfflineVibration
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Re: Weird colonization in my monotub. Why? [Re: sof4r0ckeRs1984]
    #15759772 - 02/04/12 10:14 AM (3 months, 23 days ago)

Quote:

sof4r0ckeRs1984 said:
I wondered if it was a bit too wet. As one can see the top half of the coir colonizes better then the bottom. The water follows gravitation.



This is the most logical reason I too could think of.. IF I hadn't paid attention to not make my substrate too wet.. and IF I didn't make sure that my substrate was on a dry side.

By dry side I mean 3,5 quarts (3,3L) of water per 1 brick of coir & 3 quarts of fine grade vermiculite.

All the previous times I have used 4 quarts (3,75L) of water per 1 brick of coir & 2 quarts of fine grade vermiculite.. and I hadn't had that problem.

On the 650g reptile brick I'm using it is recommended to use 3-4L of water per 1 brick.

That being said, I haven't had that dry substrate before (although the moisture content is perfect), and this is the first time something like that has happened to me.


I'm just not able to relate the problem to anything. I don't understand.


Oh and also, I sprayed LOTS of Oust (air sanitizer) into the air before spawning to bulk to make sure that there isn't much anything flying around my tub in order to minimize the probability for the mold spores to land into the tub.


It would be cool, if anyone would have ANY logical idea what's happening to the tub.


My logic says that if it's the spawn (which I highly doubt), then it wouldn't have happened like that ---> (upper half colonizing, bottom half not), because I mixed the grains extremely well into the coir % verm.


.. and my substrate is NOT too wet.


--------------------
The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.


Edited by Vibration (02/04/12 10:19 AM)


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OfflineVibration
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Re: Weird colonization in my monotub. Why? [Re: Vibration] * 1
    #15759803 - 02/04/12 10:23 AM (3 months, 23 days ago)

Funny that I had a dream tonight. In my dream I saw a new post in this thread and that was: "We have no idea".

:lol:


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OfflineSexySmurff
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Re: Weird colonization in my monotub. Why? [Re: Vibration]
    #15759818 - 02/04/12 10:27 AM (3 months, 23 days ago)

looks like you got an anaerobic region there.


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OfflineVibration
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Re: Weird colonization in my monotub. Why? [Re: SexySmurff]
    #15759852 - 02/04/12 10:41 AM (3 months, 23 days ago)

What does anaerobic mean? Lack of oxygen? If so, then what might be the cause?


--------------------
The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.


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OfflineVibration
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Re: Weird colonization in my monotub. Why? [Re: Vibration]
    #15760233 - 02/04/12 12:23 PM (3 months, 23 days ago)

One thing to be repeated: the grains on the bottom have zero growth on them.

I'm thinking could it be caused by the residue from washing the tub with bleach?? Although I washed it off with water and later wiped with alcohol too..

:eek: come on people.


--------------------
The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.


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InvisibleTranscendingLifeS
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Re: Weird colonization in my monotub. Why? [Re: Vibration]
    #15760273 - 02/04/12 12:28 PM (3 months, 23 days ago)

Give it more time.
Everything sounds like you did it all right.
If anything, your moisture content is too low.
I use a little over 1 gallon of water per Damion5050's tek. (+/- 4oz of water)


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OfflineVibration
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Re: Weird colonization in my monotub. Why? [Re: TranscendingLife]
    #15760315 - 02/04/12 12:35 PM (3 months, 23 days ago)

Ok, thanks. I think the moisture content is slightly too low indeed. I wanted to experiment with a more dry substrate this time. When I squeezed the substrate, I had to squeeze it hard to get droplets of water :smile:

I'll post updates in few days.


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Offlinetoxinn
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Re: Weird colonization in my monotub. Why? [Re: Vibration]
    #15760331 - 02/04/12 12:40 PM (3 months, 23 days ago)

Take the tape off the holes and use polyfil right from the get go. Just a lil piece of advice from a friend of mine that sped up colonization a bit. You can go heavy on the polyfil for the first ten days, but i just leave it normal.


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InvisibleTranscendingLifeS
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Re: Weird colonization in my monotub. Why? [Re: toxinn]
    #15760349 - 02/04/12 12:43 PM (3 months, 23 days ago)

Taping the holes is fine.
You want extremely high CO2 during colonization, 10k-20k PPM.
If you're using poly in the holes, it should be extremely tight & saran wrap the lid.
Vibration is doing what a lot of us do. Using the lid for GE


--------------------
AMU: We Quickly Answer Questions Here
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
:wow:How I Do EVERYTHING:wow:
“Your mind will answer most questions if you learn to relax and wait for the answer.” -William S. Burroughs
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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OfflineSexySmurff
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Re: Weird colonization in my monotub. Why? [Re: TranscendingLife]
    #15760418 - 02/04/12 12:59 PM (3 months, 23 days ago)

Quote:

TranscendingLife said:

Vibration is doing what a lot of us do. Using the lid for GE





how do you know that?  by day five if the grains aren't at least looking recovered something is definitely amiss.

and it does appear to me that he has a layer of water on the bottom.


--------------------
Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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InvisibleKizzle
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Re: Weird colonization in my monotub. Why? [Re: SexySmurff]
    #15760938 - 02/04/12 03:00 PM (3 months, 22 days ago)

It does look like it might be too wet. You can notice that coir looks darker like that when it's water content is high, and that just happens to be where it's not colonizing.


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Re: Weird colonization in my monotub. Why? [Re: Kizzle]
    #15760969 - 02/04/12 03:08 PM (3 months, 22 days ago)

If the substrate is not fluffy enough, it can do that. Bac/mold contam can also cause it to stall.

Either way there's nothing to do but wait, and you'll still get some mushrooms in the end. It's kinda funny, but my best flush ever was about 90% colonized with trich. All is well that end's well.


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OfflineDiesel_Dawg
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Re: Weird colonization in my monotub. Why? [Re: maug]
    #15761561 - 02/04/12 05:38 PM (3 months, 22 days ago)

Screw the bottom, once the top is colonized, fruit it.


PS: it does look like water accumulation and compaction on the bottom is causing your problem.


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OfflineVibration
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Re: Weird colonization in my monotub. Why? [Re: Diesel_Dawg]
    #15762085 - 02/04/12 07:48 PM (3 months, 22 days ago)

Ok, I'm going to re-mix the substrate. I hope that's going to help. It's pointless just waiting. It's not colonizing on the bottom.


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The trick is to combine your waking rational abilities with the infinite possibilities of your dreams. Because, if you can do that, you can do anything.


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Offlinezepski
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Re: Weird colonization in my monotub. Why? [Re: Vibration]
    #15762890 - 02/05/12 12:13 AM (3 months, 22 days ago)

Remix? its only been 6 days.  wait another 4 i would say min...Prolly another 8 lol


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Invisiblemaug
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Re: Weird colonization in my monotub. Why? [Re: zepski]
    #15762925 - 02/05/12 12:23 AM (3 months, 22 days ago)

word ^^

Don't jump the gun.


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I think nighttime is dark so you can imagine your fears with less distraction. -Calvin and Hobbes


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