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 RatherBeInTheWoods
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 Pruning Gymnopilus?
#15736641 - 01/30/12 12:20 AM (3 months, 28 days ago) |
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So Ive harvested the larger developed caps by cutting the stem back to the point where there are new pins coming out.
Im going to get some pics of this tomorrow.
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So my question is would harvesting before the veil has broken, force more energy into developing more pins because it wasnt able to drop spores?
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 Swan Song
Registered: 02/08/06
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it sounds logical however I am not seasoned enough to speak with authority on this subject. you might try contacting some of the mods like RR or Prisoner1 or even PMing one of the trusted cultivators here. Another avenue would be to check with the guys in advanced mycology on the subject. Most of which have moved on to harder stuff than your run of the mill grows.
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PureHash
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Re: Pruning Gymnopilus? [Re: Swan Song]
#15737274 - 01/30/12 07:39 AM (3 months, 28 days ago) |
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I recall RR saying that when a mushroom sporeulates it has reproduced, And when it reproduces the fungi doesnt have a reason to live anymore (So to speak) So you may have troubles getting it to fruit aain if it is an isolated set of genetics.
I have seen this on small trays that i matured for spore prints.. They just didnt want to fruit after that, Mold eventually took over.
I have seen this consistently with isolates on a few different trays.
This B+ Tray only fruited once
vvv
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slapphappypill
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Re: Pruning Gymnopilus? [Re: PureHash]
#15737430 - 01/30/12 08:53 AM (3 months, 28 days ago) |
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I've seen the exact opposite of what Purehash is talking about.. I've had trays/tubs where every fruit has dropped all it's spores, and covered the bottom completely in spores. After a dunk, the tub has produced the almost the same as the 1st flush, and 3rd and 4th flushes have followed each time. I've never gotten contams...
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RatherBeInTheWoods
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So it may do more harm, just on the basis of an infection being introduced.
As far the wild goes it may be best to just monitor the growth and just choose the right moment to take the mature ones as well as the pins down to their bases in order to have a greater yield by not losing the older specimens to decay or bugs.
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Being that it is already done, I guess well just see. Hopefully Ill get a moment to go out back and take some pics. Last I checked the pins were still pushing up and becoming more meaty. Where the cuts were made insects seemed to be drawn in,hopefully I did'nt ruin the colony.
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RatherBeInTheWoods
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Well its been a few days And I havnt seen any new pins develop. I have some pics that should be up on the 5th.
It looks to me that there is more decomposion more than anything else. Its been super dry around here, so I figures myabe adding some water should help things out.
This seems to also be making things worse.
The bugs are loving it though. Some large ants moved in under the patch and various fly and gnats doing their ritual on it
I am learning from this which is good I just dont want to destroy the colony so it will fruit again.
Should I just let things be and let it rot or salvage what there is to salvage by cutting everything at ground level and covering it back up with leaves and such.
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RatherBeInTheWoods
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After read reading your post PureHash, I thought of taking tissue from another colony and say splice it into this one would this be considered {breeding} and therefore produce more fruit.
Or I have some bits and pieces from another colony that have started to form small masses of Mycelium on some crush wood. I have also taken tissue fromanother colony and havent looked at but it should have also be seeing the same results soon. Would putting the "spawn" of these to culture together be the thing to do.
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slapphappypill
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Quote:
slapphappypill said:
I've seen the exact opposite of what Purehash is talking about.. I've had trays/tubs where every fruit has dropped all it's spores, and covered the bottom completely in spores. After a dunk, the tub has produced the almost the same as the 1st flush, and 3rd and 4th flushes have followed each time. I've never gotten contams...
Just to reiterate my point... I have a mono that is finishing it's 4th flush now. I've let the fruit drop spores EACH flush, and myc is completely covered in spores and fruiting JUST fine. I see no proof of spores causing contamination! and am still receiving a very strong 4th flush
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Munchauzen
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slaphappy is right. nothing about the spores themselves cause the contamination.
but what does happen is that when the mushrooms sporulate, it tells the mycelium the life-cycle is complete and it begins to die back, becoming more susceptible to contaminations.
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RatherBeInTheWoods
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This is in the wild though. I was refering to the open tissue being more prone to contamination, I wasnt worried about the spores themselves.
I was thinking along the lines of what happens when you prune say a flowering plant. Often what happens is the plant will form two or more buds where the one was cut.
Well anyway what happened was it was eaten from the inside out by larve. The pins were aborted and it seemed to be putting energy else where. I cut the whole thing off at ground level and dissected it. It was ravaged to say the least,and there wasnt to much to salvage. I think it would have been best to harvest by cutting at ground level to begin w/.
But anyway here are the pics. You cant tell from the pics but these pins are not growning. They are actually being eaten on the inside.
I really just want to make sure the colonies that I am harvesting are managed correctly so they will fruit again in the future.
Thanks everyone fore your input it keeps me thinking outside myself.
http://
Edited by RatherBeInTheWoods (02/06/12 10:10 AM)
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Munchauzen
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took me awhile to figure out exactly what you were asking... but..
when harvesting wilds, its said you shouldn't take all the fruits because you need to leave some behind to sporulate and spread the species. harvesting fruits doesn't "kill" a colony. weather conditions do, and a lot of mushrooms are seasonal for that reason.
also, mushroom fruitbodies are 100%, and if left attached to a substrate, will not rot. it will be converted back into fluffy mycelium, instead of reproductive mycelium.
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RatherBeInTheWoods
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Thanks for the info. By fluffy mycelium you mean it will be basically be converted back into the main mycelium structure{the nutrient gathering network} that is beneath the fruit body?
Sorry if I am hard to understand Im half scientific minded half backwoods. I need to read up some more
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Munchauzen
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yes thats exactly what I meant, you understood correctly.
its all mycelium, it just (somehow) can repurpose itself for different uses.
we tend to think of things as linear. you chop off your nose, it doesn't grow back. you grow a chicken from an egg, you can't turn that chicken back into an egg. mushrooms don't really abide by this. and this is why cloning and genetic isolation is so easy almost anyone with agar can refine their genetics.
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RatherBeInTheWoods
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Im not there yet but I think I have a basic understanding, I just need to spend more time researching.
I do have another question for you though.
Say you have two separate colonies.
Can you combine two tissue samples to form a new strain by letting the mycelium grow together?
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slapphappypill
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As long as they're the same type of mushroom, yes. For instance, you cannot combine a pan cyan and a cube, but you can combine 2+ types of cubes together no problem
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We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google!
Side note: Yes i've been away for a while, but I have turned into more of a lurker than anything the last few months. I'll be soon posting my most recent projects, so stay tuned within the next few weeks!
Currently looking for a AA #75X Pressure cooker, please PM me if your trying to get rid of yours! ONLY ESTABLISHED TRADERS PLZ
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RatherBeInTheWoods
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I was hoping that could be done. Thats pretty awesome and fairly easy at the same time. Thanks
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Munchauzen
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heres a good video for you
http://www.mushroomvideos.com/Strain-Isolation
http://www.mushroomvideos.com/Mushroom-Cloning
unfortunately, the segment about crossing two varieties isn't available on the website. however, I can tell you that if the two strains mix and intertwine on the agar, they are compatible. if they are not, they will create an area of separation, where its clear that they are not growing together, but rather resisting each other.
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RatherBeInTheWoods
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Thanks for the links Im pointed in the right direction.
I think its going to take a while before I'm there though. Right now the kids take most of my time. I def. have dreams pointing in that direction though. Everything I have done has been un-sterile and basically just thrown together. Although something very cool happened the other day. I gave up on this one culture that was just chopped grass/straw combined with various seeds from around my property. I left it on the porch and when I came to clean it up it was fruiting. So there is hope.
Anyway thanks again.
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PureHash
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Quote:
RatherBeInTheWoods said:
After read reading your post PureHash, I thought of taking tissue from another colony and say splice it into this one would this be considered {breeding} and therefore produce more fruit.
Or I have some bits and pieces from another colony that have started to form small masses of Mycelium on some crush wood. I have also taken tissue fromanother colony and havent looked at but it should have also be seeing the same results soon. Would putting the "spawn" of these to culture together be the thing to do.
no you would need 2 sets of mono mycelium of the same species to "breed" its not worth the effort. just isolate a good set of genes that fruit well. this can take time tho.
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RatherBeInTheWoods
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Re: Pruning Gymnopilus? [Re: PureHash]
#15776539 - 02/07/12 08:46 PM (3 months, 19 days ago) |
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After researching a while it did seemed to be more complicated than I was hoping.
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slapphappypill
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and by doing that, yeah, you might get a super potent fruit, however you'll have a bunch of issues with pinset and getting any real weight from a flush. X7X is a good example of the + and - of playing mad scientist 
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We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google!
Side note: Yes i've been away for a while, but I have turned into more of a lurker than anything the last few months. I'll be soon posting my most recent projects, so stay tuned within the next few weeks!
Currently looking for a AA #75X Pressure cooker, please PM me if your trying to get rid of yours! ONLY ESTABLISHED TRADERS PLZ
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RatherBeInTheWoods
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How long would a isolated set of genetics last before you would have to start over?
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slapphappypill
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depends, but should last a few good generations. I noticed decline on an F+ strain starting on the 5th generation
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We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google!
Side note: Yes i've been away for a while, but I have turned into more of a lurker than anything the last few months. I'll be soon posting my most recent projects, so stay tuned within the next few weeks!
Currently looking for a AA #75X Pressure cooker, please PM me if your trying to get rid of yours! ONLY ESTABLISHED TRADERS PLZ
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RatherBeInTheWoods
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Is that starting from spores or a clone?
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slapphappypill
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I'm not sure... It was a plate of agar I got by accident... not sure if it was from spore, clone, isolate, or what lol
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We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google!
Side note: Yes i've been away for a while, but I have turned into more of a lurker than anything the last few months. I'll be soon posting my most recent projects, so stay tuned within the next few weeks!
Currently looking for a AA #75X Pressure cooker, please PM me if your trying to get rid of yours! ONLY ESTABLISHED TRADERS PLZ
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RatherBeInTheWoods
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Thanks for your help man.
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PureHash
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Quote:
RatherBeInTheWoods said:
How long would a isolated set of genetics last before you would have to start over?
Grow out a master dish(p1), And use this to knock up a second dish. (p2) Use the second dish to do your A2G, Making LC's etc. Whe this dies, Or you run out, Go back to your master dish(p1) and grow out another P2..
Should last quite some time if you keep it in good conditon, and the propper inviroment.
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RatherBeInTheWoods
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Re: Pruning Gymnopilus? [Re: PureHash]
#15812872 - 02/15/12 09:25 AM (3 months, 12 days ago) |
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Yea I definitely want to move to agar. I don't even have a pressure cooker right now. I have been doing everything pretty primitively, although I have still had some fruits some how. I think I have found a place to get it{native sun}, a local health food store. I just need to get all the supplies need.
I feel like when I make this switch things are going to explode for me.
I get the concept your talking about though. I guess you would store this master culture say in a fridge. I havent really thought of this. Thanks for the info.
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