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Post Office Tyvek Advocator
Registered: 10/03/04
Posts: 8,352
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 Spores under a Microscope!  2
#15493607 - 12/09/11 10:30 PM (5 months, 17 days ago) |
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Figured I would start a thread of "Spores under a Microscope!"...
Trying to use some of these spores as "intended"...
Feel free to post your own pics, or comment on mine!
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total
Post Office Tyvek Advocator
Registered: 10/03/04
Posts: 8,352
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Re: Spores under a Microscope! [Re: total]
#15493629 - 12/09/11 10:33 PM (5 months, 17 days ago) |
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Some shots taken @ 400x, with a camera aimed straight down the barrel! 
2nd picture of both these, appear to be germination of spores w/ mycelium...
B+
 
P. mexicana var. Jalisco
 
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 audiophoenix
Forever Young
 
Registered: 08/28/09
Posts: 3,138
Loc: Upstate NY
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Re: Spores under a Microscope! [Re: total]
#15493670 - 12/09/11 10:42 PM (5 months, 17 days ago) |
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out of curiosity, what is the purpose for viewing spores under a microscope other than perhaps identification and entertainment? Is there any real advantage to be take by using a microscope in this hobby?
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total
Post Office Tyvek Advocator
Registered: 10/03/04
Posts: 8,352
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ID'ing is prob. the biggest use...
Along with checking cultures for contamination...
I find it entertaining none-the-less...
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audiophoenix
Forever Young
Registered: 08/28/09
Posts: 3,138
Loc: Upstate NY
Last seen: 3 days, 5 hours
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Re: Spores under a Microscope! [Re: total]
#15493692 - 12/09/11 10:48 PM (5 months, 17 days ago) |
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yeah not saying I don't understand why people have them. I wish I could get one right now, but is it really useful for contams in agar? I might have to look into that.
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total
Post Office Tyvek Advocator
Registered: 10/03/04
Posts: 8,352
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In Agar?
Contams should be obvious "on" agar...
You can use your microscope to see contams inside your spore syringes!:super2:
Im just enjoying my new toy...
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audiophoenix
Forever Young
Registered: 08/28/09
Posts: 3,138
Loc: Upstate NY
Last seen: 3 days, 5 hours
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Re: Spores under a Microscope! [Re: total]
#15493732 - 12/09/11 10:55 PM (5 months, 17 days ago) |
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Oh cool I see. I will keep my eye on this thread, Would like to see what a contam looks like in a spore solution.
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ShadOWCrack
~~~LOOKING DOWN THE SCOPE~~~
Registered: 07/18/10
Posts: 7,601
Loc: EVERY WHERE
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i want to see the fal bro!!!!!
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 9,507
Loc: Canada 
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Re: Spores under a Microscope! [Re: ShadOWCrack]
#15494329 - 12/10/11 05:06 AM (5 months, 17 days ago) |
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Psilocybe azurescens
12X16=192X Magnification
12X40=480 Magnification
Correction Factor X Divisions on reticle = 2.47um X5=12.35 micrometers (um) spore size
 
12X100=1200X Magnification (Oil Immersion)
 
Panaeolus cambodginiensis - Suphanburi Goliath
12X16=192X Magnification
12X100=1200X Magnification (Oil Immersion)
Correction Factor X Divisions on reticle = 1um X11=11 micrometers (um) spore size
 
Psilocybe weilli
12X40=480 Magnification
12X100=1200X Magnification (Oil Immersion)
 
Psilocybe franciscans
12X16=192 Magnification
12X40=480 Magnification
12X100=1200X Magnification (Oil Immersion)
Pholiota nameko
12X40=480 Magnification
Correction Factor X Divisions on reticle = 2.47X2=4.94 micrometers (um) spore size
12X100=1200X Magnification (Oil Immersion)
 
Gymnopilus Species (From Stonesun)
12X40=480 Magnification
12X100=1200X Magnification (Oil Immersion)
Correction Factor X Divisions on reticle = 1X8=8 micrometers (um) spore size
  
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Javadog
Continuing along
Registered: 05/03/10
Posts: 6,055
Loc: USA 
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Great stuff guys.
EM, how do you compute the "Correction Factor"?
Thanks!
JD
--------------------
Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 9,507
Loc: Canada
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Re: Spores under a Microscope! [Re: Javadog]
#15494702 - 12/10/11 08:28 AM (5 months, 17 days ago) |
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The gentleman who sent me the scope calibrated the scope with a stage micrometer.
This gives you a number to X by the scale on a reticle for accurate sizing
of specimens under the microscope.
He sent me all the numbers for each of my objectives, so when I measure a
spore for example I multiply the divisions by the correction factor of that
objective to find the size of the spore in micrometers.
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Javadog
Continuing along
Registered: 05/03/10
Posts: 6,055
Loc: USA
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I see. I must have gotten similar info with my scope.
I need to get busy with that thing, but am occupied these days.
Thank you for taking the time,
JD
--------------------
Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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mayhem250
Professional Bullshitter
Registered: 01/26/06
Posts: 11
Loc: Michigan
Last seen: 5 months, 6 days
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P. Mexicana..., want to trade a print? [Re: total]
#15494737 - 12/10/11 08:45 AM (5 months, 17 days ago) |
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not the place for trading
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You know what..."you just can't fix stupid, you can't" some people are just fuct!
Edited by Prisoner#1 (12/10/11 01:18 PM)
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total
Post Office Tyvek Advocator
Registered: 10/03/04
Posts: 8,352
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Re: Spores under a Microscope! [Re: ShadOWCrack]
#15497047 - 12/10/11 06:08 PM (5 months, 16 days ago) |
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Quote:
ShadOWCrack said:
i want to see the fal bro!!!!!
Falbino (White spores from stonesun...)
 
Both shots taken @ 400x, with some camera zoom, straight down the barrel!
Edited by total (12/10/11 06:09 PM)
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bfogg8706
Stuntzii radar dish
Registered: 09/04/09
Posts: 2,928
Loc: Washington State
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Re: Spores under a Microscope! [Re: total]
#15497749 - 12/10/11 09:13 PM (5 months, 16 days ago) |
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Panaeolus sp. at 200X 
Panaeolus acuminatus @ 2000X 
A mutated Amanita pantherina spore at 2000X 
Inocybe sp. @ 800X 
I may add more when I get some time.
--------------------
Proud supporter of the Shroomery!!
Looking for exotic spores!!! Random Woodlover Projects
    
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total
Post Office Tyvek Advocator
Registered: 10/03/04
Posts: 8,352
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Re: Spores under a Microscope! [Re: bfogg8706]
#15497869 - 12/10/11 09:49 PM (5 months, 16 days ago) |
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Was the "Panaeolus sp. at 200X" using a stereo microscope?
Thanks for the wonderful pictures btw!
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bfogg8706
Stuntzii radar dish
Registered: 09/04/09
Posts: 2,928
Loc: Washington State
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Re: Spores under a Microscope! [Re: total]
#15497967 - 12/10/11 10:13 PM (5 months, 16 days ago) |
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No, I actually just clamped my headlamp so it would shine onto the slide I was working with. Good idea for a thread
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roaddog
turnip
Registered: 09/29/09
Posts: 2,011
Loc: third rock from the sun
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Re: Spores under a Microscope! [Re: bfogg8706]
#15497999 - 12/10/11 10:23 PM (5 months, 16 days ago) |
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great thread. cool equipment, I have been thinking about getting one, so I can view trichomes, without using one of those little radio shack 100 times hand microscopes. It would be way cool to view spores from wild strains, too.
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TimmiT
Registered: 03/23/10
Posts: 2,431
Loc: Victoria 
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Re: Spores under a Microscope! [Re: total]
#15498990 - 12/11/11 07:58 AM (5 months, 16 days ago) |
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Psilocybe subaeruginosa from last year
Coprinus sterquilinus from last week
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"Reality leaves a lot to the imagination" ~ John Lennon
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hamloaf
Cannonball Freight Train.
Registered: 12/23/09
Posts: 6,524
Loc: The Electric Guitar.
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Re: Spores under a Microscope! [Re: total]
#15499499 - 12/11/11 10:31 AM (5 months, 16 days ago) |
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I'm feelin' this thread. 
--------------------
[quote]Enlil said:
[quote]hamloaf said:
You can't mail cutless enlil's address because you don't have it and, are a lying piece of shit and, in general a manipulative and bad person.[/quote]
QFT
And hamloaf is a guy I genuinely dislike![/quote]
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