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 CTHULHU007
Stranger
Registered: 10/15/11
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Last seen: 1 year, 6 months
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 Healthy Mycelium, but a little light yellow/brown COLORATION in areas? 
#15227848 - 10/15/11 01:35 AM (1 year, 7 months ago) |
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Hello,
I had an account here about 5-6 years back, but apparently it's lost or deleted for inactivity..no problem there I was busy with other things in life.
Anyway, I'm no novice to mushroom cultivation, however it's been about 4-5 years.
If one were to have healthy uncontaminated mycelium in some 2 week old inoculated jars that was starting to yellow or brown very lightly ON top of the mycelium what might that be? It's not a separate mold I KNOW. It's the color of the mycelium itself varying a little from pure white mostly where the mycelium is oldest on the inoculation points spreading out. The growing edges and majority of it is normal bright white and is VERY healthy looking. All was sanitary during part 2 inoculation..as I mentioned I'm no spring chicken when it comes to this.
I know for a fact I've had this situation before and I believe it wasn't a big deal...I believe, but I forget the details.
It's in almost all..maybe all of the pint jars that are near fully colonized and some that still have a ways btw. Perhaps a bacteria?(hope not...though I killed some once I recall with a bleach dunk) A metabolite? Water damage? The pics are very hard to tell from. It's just a very light surface coloring of the mycelium yellow-brownish. Note the pure white picture perfect look of the photo made with the flash on. It's not that noticeable in the pics.
Assistance is ALWAYS appreciated.
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migraineur
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 Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: CTHULHU007]
#15227906 - 10/15/11 01:57 AM (1 year, 7 months ago) |
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I can't really see much from those pictures but is it a yellow like liquid that you see? If so then it's most likely metabolites.
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Fatwa on you!
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CTHULHU007
Stranger
Registered: 10/15/11
Posts: 8
Last seen: 1 year, 6 months
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: migraineur]
#15228087 - 10/15/11 03:44 AM (1 year, 7 months ago) |
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Not a liquid that I can see unless it's mist sized particles. No I don't think it's liquid. I've had this happen before. I don't think it was much trouble I just forget what the Hell it was causing it.
Drying maybe? The mycelium is healthy and growing just the older mycelium is a little off white yellow/brown.
I know it's very hard to see in the pics. The first one you can kind of see it in the center line of the jar near, but before the darkened area of the jar at bottom. The mycelium just looks tan in all of them on the oldest mycelium..which is at the inoculation points mostly, but not limited there. They are pretty warm and have condensation inside the jars=moisture loss.
I hope it's mycelium piss...or drying..or water damage. I recall a bacteria once long ago that made the cakes smell like shite and piss yellow fluid. I killed that with bleach. This isn't the same, but I've seen it before I KNOW. It has to be enviroment or bacteria. I hope not bacteria..I think not bacteria..just don't recall...
The vermiculite used was EXTREMELY fine and had finer dust in it...I wouldn't think that would color the mycelium, however. I've seen and read a few other posts about this very effect with no real conclusion reached in any that I saw.
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 Kizzle
Misanthrope
Registered: 08/30/11
Posts: 4,398
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: CTHULHU007]
#15228335 - 10/15/11 07:04 AM (1 year, 7 months ago) |
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They're still colonizing so that always a good sign. If it is bacteria it shouldn't take long before it becomes obvious that's what it is.
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CTHULHU007
Stranger
Registered: 10/15/11
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Last seen: 1 year, 6 months
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: Kizzle]
#15230293 - 10/15/11 06:53 PM (1 year, 7 months ago) |
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Actually years ago I had bacterial infection and the cakes fully colonized. They just didn't fruit and made mutated mushroom tissue cancer shaped like blobs. They smelled like fecal matter kind of back then. I bleach dunked them and the cancer turned into fruits.
I just forget what the Hell this is. This isn't the same as that incident. It is the same as another incident or two..or three from years ago that I cannot recall the resolution to.
I know some on here have seen it, because I've seen posts about the exact same thing...without resolution, however. Is anyone around here that's had this before and been through it to fruiting? I think myself it's just discoloration..caused by water, drying, or ..something else. Aye, it is still growing healthy, but the yellow patches spread like drying. I note the cake is squeezing away from the jar whereever there is yellowish-brown stain. It reminds me of old 4th flush cakes that are too dry..the color they get.
Is there no one present here that has seen this before?
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 4,398
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: CTHULHU007]
#15230402 - 10/15/11 07:23 PM (1 year, 7 months ago) |
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If the cake is contracting it's consuming the substrate, which might explain the excess metabolites if that's what they are. I've heard that while colonizing if there is too much gas exchange the mycelium will consume more of the sub instead of simply colonizing it. 
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CTHULHU007
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Registered: 10/15/11
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Last seen: 1 year, 6 months
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: Kizzle]
#15231681 - 10/16/11 12:54 AM (1 year, 7 months ago) |
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Well with upside down jar lids even with tape still over them they should be getting plenty...or enough gas exchange. It's kind of a dry area, however they are in..but heated to about 82-84 F*.
I looked closer under magnifying glass at the areas and I see rhizomorphic tendrils that are yellowish tinted. There is nothing at all on the mycelium growing it's just the mycelium itself that's changing color tint except in areas of fairly new growth where it is white as snow still especially at the edge of colonization where it's most rhizomorphic..pure white.
This may or may not be an issue, but those jars were stored for about 5 years in the closet of a smoker and smoke had fairly free reign of the closet. When cleaning the inside of a jar early on the towel used turned brownish yellow. That was the only jar that was cleaned...the others were just sterilized with substrate in them. Don't know now if it's the tobacco stain coming off the jar with the mycelium contracting. I know a lazy potentially disaster technique, but sterilization was complete with the jars. Still have an un-inoculated jar that is fine after two weeks.
This happened before though I know..at least something similar. I'm going to guess mycelium metabolites or discoloration due to the inside of the jars being stained and not cleaned fully until someone says otherwise or it's proved. Could be bacteria, but I don't see how. Not in all of them..
Thanks for the replies. I wish someone who's seen this would come in here and remind me what this is for sure. Alas.
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 4,398
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: CTHULHU007]
#15232048 - 10/16/11 02:26 AM (1 year, 7 months ago) |
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Well, now you've seen it. So if nothing else you can tell us how it turns out. I think you're probably right that if it looks like discolored mycelium that's probably what it is.
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CTHULHU007
Stranger
Registered: 10/15/11
Posts: 8
Last seen: 1 year, 6 months
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: Kizzle]
#15232270 - 10/16/11 03:41 AM (1 year, 7 months ago) |
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I've researched more on mycelium metabolites and I'm thinking that this MAY be it as it was noticed a very small drop of amber fluid in one jar on the mycelium that was discolored. It could be a combination of both metabolites and staining. I think the tape should be removed and the temp lowered perhaps.
The mycelium in these jars looks great aside from the amber/yellowish areas that look a little dry, but otherwise normal. It may be growing conditions..the heat got a little warm (90*F) for about 6 hours once and they've been at 84 for the entire time. That means the jar temps are 85*-86*+.
Forgot how annoying the little details can be when something looks to go wrong. I hope they can be salvaged. Whenever metabolites(amber fluid drops) were seen before the cakes didn't fare to well and made more mutants than anything else...also contaminating out after 2-3 flushes(normally 4-5).
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CTHULHU007
Stranger
Registered: 10/15/11
Posts: 8
Last seen: 1 year, 6 months
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: CTHULHU007]
#15236961 - 10/17/11 04:32 AM (1 year, 7 months ago) |
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A new development has occurred with the cakes. After un-taping two holes each jar and lowering the temp to 74 the yellow Mycelium is seemingly growing white again and bursting with even more growth than before. I just did this a couple of days back. The one jar photo I posted here was amber in the most areas and widest spread, but now the amber colored Mycelium areas are shrinking and hopefully disappearing.
Looking 10 times better now so far...if anyone else cares to even notice my post here aside from Kizzle. May be an enviromental factor..too much heat to little gas exchange.
Regardless it will be known what it was/is later this week as some should be done by then.
I've noted Mycotopia forums seemed much more helpful..no offense Kizzle, but you are the ONLY person here to apparently even feel this thread was even worth replying to. I appreciate that.
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 4,398
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: CTHULHU007]
#15237196 - 10/17/11 07:55 AM (1 year, 7 months ago) |
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I may not be much help but I get curious and want to figure out what the hell is going on too.
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CTHULHU007
Stranger
Registered: 10/15/11
Posts: 8
Last seen: 1 year, 6 months
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: Kizzle]
#15253666 - 10/20/11 09:09 PM (1 year, 6 months ago) |
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Quote:
Kizzle said:
I may not be much help but I get curious and want to figure out what the hell is going on too.
Every time someone gives an opinion it's helpful. 
I think it is either a bacterial contam that was incubating fast at higher temps and metabolites were fighting it or ...just excess metabolites seeping out. Cakes were more wet than they should have been anyway.
HOWEVER, now that air exchange is increased and temperature lowered most of them look healthy white now. The amber/yellow mycelium has went mostly white in all but a few jars that have a few spots left that are being overtaken by white...and that's the worst jars..or rather the jars that looked worst.
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Tyanogy
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Re: Healthy Mycelium, but a little light yellow/brown COLORATION in areas? [Re: CTHULHU007]
#16175137 - 05/03/12 04:22 AM (1 year, 18 days ago) |
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your assumptions that it is due to bacteria is correct. prolonged exposure to bacteria will cause mycelium to turn yellow and eventually turn darker and harden to form into something like a scab. ive seen it happen in bacteria contaminated liquid cultures, and ive seen it happen to mycelium that has grown over the verm filter layer where the holes in the lid are.
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