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 luckee8989
Stranger
Registered: 08/03/11
Posts: 175
Last seen: 10 years, 5 months
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 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) 
#15078674 - 09/14/11 03:03 PM (12 years, 7 months ago) |
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So, I have 9/12 jars that made it through to near 100% colonization and had to throw out 3 to contams. They colonized extremely quickly in a darker place with some light (cardboard tray on side shelf of an open closet facing the window) and in ~68-70 deg F. Unfortunately we keep our place pretty cool, but I try to turn up the thermostat when no one is home.
They've probably continued colonizing only a small amount, if at all, this past week. However, I expected much whiter, more solid mycelium than this. Additionally, one of my jars has already started pinning and growing invitro (below)! There are some pins on a couple others too. Is it okay to keep waiting just a few more days to birth them all at the same time? The less colonized jars:
Also, the yellow on this myc worries me. It's just metabolites, right? It's been there for a good while and doesn't seem to be going away. The metabolites on the left and the fast pinner on the right:
Also...will temps of ~65-68 really be terrible?
Thanks for any help! I'm having a blast!
Edited by luckee8989 (09/14/11 03:10 PM)
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IntotheMystic
Seeker
 
Registered: 08/12/11
Posts: 48
Last seen: 12 years, 4 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: luckee8989]
#15078776 - 09/14/11 03:38 PM (12 years, 7 months ago) |
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I stuck a heating pad in the space with mine and got the temp up to about 80 but BE CAREFUL not to get them too hot...and I have no idea about the yellow stuff I'm new to this too
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"We'll ride the spiral to the end we may just go where no one's been"
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trophycase
Dickface
Registered: 03/23/11
Posts: 1,018
Last seen: 5 years, 11 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: luckee8989]
#15078843 - 09/14/11 03:55 PM (12 years, 7 months ago) |
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That jar on the left don't look quite right to me... I would toss it. Most of the pics I've seen of metabolites are small little drops, and I believe that metabolites are a reaction to bacteria/contams competing with the myc... It could be metabolites, but there's definitely something a little out of the ordinary with that jar.
Wait for others to chime in, as I haven't really had experience with metabolites... I'm not saying it's for sure a contam, it just doesn't look quite right.
And don't worry about the pins. Let that bad boy finish colonizing and throw it into the FC. 
P.S. Your temps should be OK, but not prefered, and will probably be al ittle slower than the norm.
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Lord_McLovin
mad scientist on shrooms
Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
Last seen: 6 years, 3 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: trophycase]
#15078973 - 09/14/11 04:55 PM (12 years, 7 months ago) |
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At least 25% of your jaws contaminated. That means you're doing something wrong.
Quote:
However, I expected much whiter, more solid mycelium than this.
Don't worry about it. It's MS culture. 
Quote:
Additionally, one of my jars has already started pinning and growing invitro (below)!
It might be contaminated. Early fruiting is usually caused by contamination. You can either keep it in the jaw and hope that it will reach 100% or toss it.
Quote:
Is it okay to keep waiting just a few more days to birth them all at the same time?
A few days won't do much harm.
Quote:
Also, the yellow on this myc worries me. It's just metabolites, right?
It might be contaminated with bacteria. If it reaches 100% don't worry about it either.
Quote:
Also...will temps of ~65-68 really be terrible?
75°F would be better, but it will just slow down the metabolism of the myc. You'll be fine.
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 Fungus_monk
Here or there...
Registered: 08/07/11
Posts: 1,589
Loc: U.S. 
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Lord_McLovin]
#15079025 - 09/14/11 05:06 PM (12 years, 7 months ago) |
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Where have you seen that early pinning is a sign of contamination? Let alone usually caused by contamination.
Ever jar i've had so far has pinned invitro.
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My Trade List
I post fictional information that i have developed through years of extreme delirium and from suffering strange delusions that i partake in illegal activites. The shroomery.org is merely my outlet for these delusions.
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trophycase
Dickface
Registered: 03/23/11
Posts: 1,018
Last seen: 5 years, 11 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Fungus_monk]
#15079065 - 09/14/11 05:16 PM (12 years, 7 months ago) |
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Quote:
Fungus_monk said:
Where have you seen that early pinning is a sign of contamination? Let alone usually caused by contamination.
Ever jar i've had so far has pinned invitro.
I was wondering the same thing... I've never heard anybody say that invitro pinning is a sign of contams. RR talks about invitro pins all the time in some of this notes, and never says anything about contams being the cause. Furthermore, I don't think I've ever seen somebody say that their jar with invitros got contam'd soon after... Most people leave it till full colonization and birth it with no problems... Do you have any sources?
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All of the cultivation photos uploaded by this account were taken in an area of the globe where such practices are legal and uninhibited.
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Lord_McLovin
mad scientist on shrooms
Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
Last seen: 6 years, 3 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Fungus_monk]
#15079093 - 09/14/11 05:21 PM (12 years, 7 months ago) |
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I read it in one of RR's responses. He said, that the myc tries to reproduce itself by forming fruitbodies, because it cannot fight of the contam well enough.
Unfortunately, I cannot find the exact post, only this one:
Quote:
RR said
Usually, something is the trigger for early pinning, such as bacterial contamination.
I mean it's certainly not too much FAE during colonization which causes his cake to pin. Contamination seems pretty plausible.
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Fungus_monk
Here or there...
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Posts: 1,589
Loc: U.S.
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Lord_McLovin]
#15079101 - 09/14/11 05:23 PM (12 years, 7 months ago) |
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I think i've heard of bacteria causing it on grains. But that doesnt apply to the op.
I might be wrong though, idk
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My Trade List
I post fictional information that i have developed through years of extreme delirium and from suffering strange delusions that i partake in illegal activites. The shroomery.org is merely my outlet for these delusions.
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Lord_McLovin
mad scientist on shrooms
Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
Last seen: 6 years, 3 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Fungus_monk]
#15079126 - 09/14/11 05:31 PM (12 years, 7 months ago) |
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Quote:
Fungus_monk said:
Ever jar i've had so far has pinned invitro.
Do your jaws take very long until they are fully colonized? Maybe you pack your substrate too tightly.
Quote:
I think i've heard of bacteria causing it on grains. But that doesnt apply to the op.
That's just another kind of substrate.
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Fungus_monk
Here or there...
Registered: 08/07/11
Posts: 1,589
Loc: U.S.
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Lord_McLovin]
#15079161 - 09/14/11 05:42 PM (12 years, 7 months ago) |
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No i dont pack too tight.
I use tall half pints but i think it is more determined by genetics. Its only happened to my b+ cakes.
And yes i know grains are a substrate, but i personally feel bacterial contaminations are more common on grains than on brf.
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My Trade List
I post fictional information that i have developed through years of extreme delirium and from suffering strange delusions that i partake in illegal activites. The shroomery.org is merely my outlet for these delusions.
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Lord_McLovin
mad scientist on shrooms
Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
Last seen: 6 years, 3 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Fungus_monk]
#15079185 - 09/14/11 05:48 PM (12 years, 7 months ago) |
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Quote:
Fungus_monk said:
No i dont pack too tight.
I use tall half pints but i think it is more determined by genetics. Its only happened to my b+ cakes.
To be honest, I'm not sure about this one. It might be that genetics play a role here, maybe not. I don't know.
Quote:
And yes i know grains are a substrate, but i personally feel bacterial contaminations are more common on grains than on brf.
That doesn't make sense to me. Why should bacterial contamination be more common in grains than in cakes?
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Fungus_monk
Here or there...
Registered: 08/07/11
Posts: 1,589
Loc: U.S.
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Lord_McLovin]
#15079252 - 09/14/11 06:00 PM (12 years, 7 months ago) |
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Im just telling you thats what i've seen
Im not here to argue
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My Trade List
I post fictional information that i have developed through years of extreme delirium and from suffering strange delusions that i partake in illegal activites. The shroomery.org is merely my outlet for these delusions.
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Lord_McLovin
mad scientist on shrooms
Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
Last seen: 6 years, 3 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Fungus_monk]
#15079297 - 09/14/11 06:13 PM (12 years, 7 months ago) |
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Haha.
Sorry, I did not mean to start an argument. 
I suppose bacterial contamination just becomes more visible in grains. Maybe I'm wrong though.
No, I probably am wrong. 
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Fungus_monk
Here or there...
Registered: 08/07/11
Posts: 1,589
Loc: U.S.
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Lord_McLovin]
#15079305 - 09/14/11 06:15 PM (12 years, 7 months ago) |
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Quote:
Lord_McLovin said:
I suppose bacterial contamination just becomes more visible in grains.
thats probably it, never thought of that
--------------------
My Trade List
I post fictional information that i have developed through years of extreme delirium and from suffering strange delusions that i partake in illegal activites. The shroomery.org is merely my outlet for these delusions.
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Lord_McLovin
mad scientist on shrooms
Registered: 04/09/11
Posts: 3,071
Loc: infinite dimensional void
Last seen: 6 years, 3 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Fungus_monk]
#15079312 - 09/14/11 06:16 PM (12 years, 7 months ago) |
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I think that's just speculation.
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luckee8989
Stranger
Registered: 08/03/11
Posts: 175
Last seen: 10 years, 5 months
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Re: 1st grow roller coaster: metabolites, fast pinning, uneven progress and fluctuating temps (pics) [Re: Lord_McLovin]
#15083565 - 09/15/11 04:15 PM (12 years, 6 months ago) |
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Thanks for the feedback regardless guys. Now waiting to birth until they look perfect.
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