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Offlinebik123
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Simultaneous Shiitake fruiting
    #14635030 - 06/18/11 07:16 PM (12 years, 8 months ago)

I opened around 50 of my colonized shiitake blocks, cold shock and dunk them for like 20hrs. Only around 10 have started pinning after 5 days while the rests shows no activity. Any idea? Does hitting with stick initiate it?

Im trying to fruit them simultaneously.

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InvisibleDoctor_Inoc
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Re: Simultaneous Shiitake fruiting [Re: bik123]
    #14635596 - 06/18/11 10:13 PM (12 years, 8 months ago)

Quote:

bik123 said:
I opened around 50 of my colonized shiitake blocks, cold shock and dunk them for like 20hrs. Only around 10 have started pinning after 5 days while the rests shows no activity. Any idea? Does hitting with stick initiate it?

Im trying to fruit them simultaneously.




Did you allow a month of a consolidation period?  20 hours of a soak time for fully colonized Shiitake blocks is 19 times, TOO LONG!  You want to dunk for an hour and cold shock for 24!

As far as hitting the consolidated block to initiate fruiting, IT'S TRUE!  Physical shock is a major pinning trigger for Shiitake.

Wash well the substrates when removing them from the bags, at this point, administer the beatings.  NEXT, cold-shock.  You can just slap it all around the block.  You don't HAVE to hit it with a stick.

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Offlinebik123
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Re: Simultaneous Shiitake fruiting [Re: Doctor_Inoc]
    #14639466 - 06/19/11 08:02 PM (12 years, 8 months ago)

Thanks, now i stick to just an hr of dunking!

How about simultaneous fruiting? How does one achieve that?

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OfflineNSF
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Re: Simultaneous Shiitake fruiting [Re: bik123]
    #14640960 - 06/20/11 01:41 AM (12 years, 8 months ago)

Is dunking a fruiting trigger or should you only dunk after your first flush when you are hopin for a second???


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Offlinebik123
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Re: Simultaneous Shiitake fruiting [Re: NSF]
    #14641738 - 06/20/11 08:16 AM (12 years, 8 months ago)

Depending on the weight of the block, i dunk it accordingly for 1st flush and then for 2nd.

But how does one get, lets say 200 blocks and almost all fruiting together?

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OfflineNSF
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Re: Simultaneous Shiitake fruiting [Re: bik123]
    #14641766 - 06/20/11 08:24 AM (12 years, 8 months ago)

Treat them all the same, at the same time.

Make a bulk substrate, put it into bags, PC it at the same time (commercial growers have autoclaves you can walk into, pushing several trolleys of substrate as you go).

inoculate at the same time, with the same amount of substrate.  Allow it to colonise under identical conditions (in a colonising room).

Then fluctuate humidity and expose to varied CO2 together.  Then it's more than likely they'll fruit together.


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InvisibleDoctor_Inoc
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Re: Simultaneous Shiitake fruiting [Re: bik123]
    #14642153 - 06/20/11 10:12 AM (12 years, 8 months ago)

Quote:

bik123 said:
Depending on the weight of the block, i dunk it accordingly for 1st flush and then for 2nd.

But how does one get, lets say 200 blocks and almost all fruiting together?



Strain isolation.

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InvisibleMonkeyKnifeFight
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Re: Simultaneous Shiitake fruiting [Re: Doctor_Inoc]
    #14642468 - 06/20/11 11:25 AM (12 years, 8 months ago)

Quote:

Doctor_Inoc said:
Quote:

bik123 said:
Depending on the weight of the block, i dunk it accordingly for 1st flush and then for 2nd.

But how does one get, lets say 200 blocks and almost all fruiting together?



Strain isolation.




Unless you are trying to grow your own culture from spores the OP almost certainly has an isolate already.  Assuming that's true then I would say NSF has it right.  Do the exact same thing for each block and you should get consistent results.  But for instance I fruit stuff in a mini greenhouse with a humidifier in one corner so depending on where the blocks are in the GH they will have slightly different conditions.  If you want totally consistent results you would need a pretty environmentally consistent fruiting area.

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Offlineflugelizor
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Re: Simultaneous Shiitake fruiting [Re: MonkeyKnifeFight]
    #14644407 - 06/20/11 05:45 PM (12 years, 8 months ago)

I like the story in one of Stamets' books (ggmm?)
Where he used a bunch of carts hitched together like a miniature train, and drove them all in a pond for the soak period. Then pulled out when they are done.

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OfflineNSF
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Re: Simultaneous Shiitake fruiting [Re: flugelizor]
    #14644438 - 06/20/11 05:53 PM (12 years, 8 months ago)

This is where Stamets and RR vary.  RR doesn't soak to initiate the first flush (do you RR??  I hope I've got that right) whereas Stamets soaks overnight.

Also, RR recommends a one hour soak (not 24) between the first and second flush.  So maybe experiment with a minimum of four blocks and try the different methods and see what happens.


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Offlinebik123
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Re: Simultaneous Shiitake fruiting [Re: NSF]
    #14645340 - 06/20/11 09:22 PM (12 years, 8 months ago)

So, even though all blocks are brown and opened together, for simultaneous fruiting,it also depends on when the blocks were done together?
Ive mine, with a gap of about a week for each 50 blocks.

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OfflineNSF
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Re: Simultaneous Shiitake fruiting [Re: bik123]
    #14646720 - 06/21/11 02:49 AM (12 years, 8 months ago)

Well i'm sure they turned brown at different times.  If you put them all in to fruiting conditions then they may all fruit, but i doubt it and their yields would vary wildly.

It's like you are asking "the woman is pregnant, why does she have to give birth in 40 weeks and not now?"

Well there's a lot of development going on in the organism!


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Offlinebik123
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Re: Simultaneous Shiitake fruiting [Re: NSF]
    #14646948 - 06/21/11 05:33 AM (12 years, 8 months ago)

Yes, you are right. They turned brown at different times.
So, what happens to the blocks which ive soaked for now or those that did not fruit? Any idea what to do?

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Offlineflugelizor
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Re: Simultaneous Shiitake fruiting [Re: bik123]
    #14650279 - 06/21/11 07:28 PM (12 years, 8 months ago)

I have the same issue right now.
I have two totally brown blocks inoculated on the same day.
Rinsed off, put in fridge for two days, Put outside in a shotgun fruiting chamber with no perlite, One fruited well, one didn't.

I figure I will just set them on a shelf for a week or two, Soak for a few hours and try again with both as if they are both on the 2nd flush.

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OfflineRogerRabbitM
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Re: Simultaneous Shiitake fruiting [Re: flugelizor]
    #14650493 - 06/21/11 08:06 PM (12 years, 8 months ago)

Quote:

Put outside in a shotgun fruiting chamber with no perlite, One fruited well, one didn't.





Next time, put them outside under some bushes for shade, with grass surrounding them for humidity.  Water with a lawn sprinkler.

I never dunk prior to first flush, preferring instead to put the correct amount of moisture into the substrate at makeup.  I also rarely dunk before second flush, instead letting the rain and/or lawn sprinklers handle it.  If necessary, they get a 1 hour dunk, no more.  After an hour, a properly made shiitake sawdust block will have doubled in weight.
RR


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OfflineBlueGrower
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Re: Simultaneous Shiitake fruiting [Re: RogerRabbit]
    #14652282 - 06/22/11 02:56 AM (12 years, 8 months ago)

interesting thread, i have been making shiitake blocks, 4 each day as my pressure cooker only has space for 2 and i make 2 batches a day.  I was wondering how to make them fruit simultaniously.  I see in this thread that it´s going to be difficult


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OfflineNSF
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Re: Simultaneous Shiitake fruiting [Re: BlueGrower]
    #14652304 - 06/22/11 03:07 AM (12 years, 8 months ago)

I can't understand why you'd want them all to fruit at the same time?!  You aren't likely to sell lots at the same time, instead you'd give steady and fresh supply to restaurants or markets.

If you have to have a bulk fruiting, then next time, do the RR thing, PC your blocks with a tyvek sleeve in them.  Then store them.  A few days or weeks worth.  Then inoculate them all at the same time.  Well, one after the other in front of your hood.


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