Liquid Culture using LME (Light Malt Extract.) - Mushroom Cultivation

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PassiveAgressive
Sleepy-_-kinoko!

Registered: 10/16/09
Posts: 924
Loc: Tueri honorare saltus

Liquid Culture using LME (Light Malt Extract.)

#14580781 - 06/08/11 04:51 PM (12 years, 10 months ago)

What's the latest and greatest meathods using Light Malt Extract? I did UTFSE and covered a lot of material. Most of it seemed old and not quite compatible with what I have on hand. What I do have on hand is an old bag of brewers LME and various other forms of sugar which differ from dextrose.

Does anyone use straight LME? Anyone ever try using MEA (Malt Extract Agar) as a liquid media?

I can't wait to use the new magnetic stirrer :smile:

Thanks for your patience and knowledge :borat:

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Thousands of candles can be lit from a single candle, and the life of the candle will not be shortened. Happiness never decreases by being shared. - Prince Gautama Siddharta, the founder of Buddhism, 563-483 B.C.

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neuron99
Stranger

Registered: 04/02/11
Posts: 95
Last seen: 6 years, 5 hours

Re: Liquid Culture using LME (Light Malt Extract.)

[Re: PassiveAgressive]
#14580938 - 06/08/11 05:20 PM (12 years, 10 months ago)

I don't have personal experience but I was just advised by another member to use LME for LC:

Quote:

todlow said:
I don't recommend sugar based LCs...just grab some (Extra) Light Malt Extract (dried) & use that. 1g per 600mL of water or .667g per 400mL of water...

Also, you need a GE hole on those lids or other wise you're going to have to use a syringe filled w/ poly to take out the solution, as it will form a vacuum in the jar & you won't be able to pull the LC out...

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Edited by neuron99 (06/08/11 05:20 PM)

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steelmonkey
Homejigger

Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada

Last seen: 2 years, 6 months

Re: Liquid Culture using LME (Light Malt Extract.)

[Re: neuron99]

1
#14581116 - 06/08/11 06:02 PM (12 years, 10 months ago)

There is no latest and greatest..1 tablespoon of LME to one quart water thats all I use and it works great :shrug:

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k00laid
NEMO

Registered: 05/03/10
Posts: 19,636
Last seen: 8 months, 20 days

Re: Liquid Culture using LME (Light Malt Extract.)

[Re: steelmonkey]
#14581315 - 06/08/11 06:56 PM (12 years, 10 months ago)

jokefox seems pretty proud of his "agar broth" though i dont see much dif besides it being "thicker"

todlow just shoots GLC into his LME LC

search for their posts. i bet theyve typed their recipe once or twice.

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PassiveAgressive
Sleepy-_-kinoko!

Registered: 10/16/09
Posts: 924
Loc: Tueri honorare saltus

Re: Liquid Culture using LME (Light Malt Extract.)

[Re: k00laid]
#14581453 - 06/08/11 07:22 PM (12 years, 10 months ago)

1 tbsp/Qt water = :rainbowdrink:

Thanks guys!

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