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OfflineILBIACCO
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Re: Grain spawn without pressure cooking [Re: ILBIACCO]
    #14393649 - 05/03/11 08:53 AM (1 year, 28 days ago)

Quote:

ILBIACCO said:
Update.I've checked my pioppino jar. Well. The stem butts are, how can i say, very "fuzzy" and they are sticking to the cardboard and the grains but the growth seems very slow.




New update.
Yes. Pioppino can grow on cardboard. The growth is not in any way comparable to the cardboard lover mushrooms, like oysters for example, but i can say that A.aegerita stem butt can slowly colonize a piece of cardboard.
While as far as the fermented corn, pioppino mycelium is still sticking on grains but i can't see a downright growth yet after two weeks from the beginning, and the mushrooms are rotting.

Ten days ago, i've started another jar with pioppino on fermented straw. It seems going well so far and if all goes as planned i will utilize only straw in the future. Probably, the very low ph (4 with the grains) is a real problem for my mushrooms.
But, as we say in Italy, "una rondine non fa primavera", ("one swallow does not make a summer"?) :wink:
I have still so many things to experiment...

Thanks Lenny.  B.Barrowsii was unknown to me.

Mycelio, the ziplock bags idea is very interesting.
This forum is as a lighthouse in the stormy sea of mushroom cultivation.:thumbup:


--------------------
I've been waiting for her for so long
Open the sky and let her come down
Here comes the rain
Here she comes again
I love the rain
Rain Rain Rain
(The Cult)


Edited by ILBIACCO (09/16/11 05:25 AM)


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InvisibleAlkaloids
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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14394192 - 05/03/11 11:16 AM (1 year, 28 days ago)

Quote:

Mycelio said:
No, I never noticed any difference in the fruitbodies.

Covering with oil should work, but it sounds unnecessarily messy. For me, fermentation in zip-lock bags is the easiest way and I never lower the PH, nor do I add any starter culture, as all the species we need are already present on grain or straw. If you think you should add something, look for sourdough starter. Whenever you try the first time, I heavily recommend fermenting small amounts and not to change too much.

Carsten





  I start with lactobacillus cultures that i cultivate from the local environment/air.  So, in essence, i am starting with something similar to wild sourdough starter cultures.  I have so much faith in my lac b serums and any resulting by-products that i eat/ingest them. :smile: It's really quite good for intestinal health.  Useful for resolving digestive irregularities as well.

  The reason i suggest oil is simply to provide more possible avenues to do the fermentation.  As a rule i avoid plastics as much as i can.  Not to mention that in many places getting plastic goods can be quite difficult or expensive.

I won't be doing giant batches as trials.  Slow and easy  :wink:


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OfflineMycelio
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Re: Grain spawn without pressure cooking [Re: ILBIACCO]
    #14398599 - 05/04/11 06:09 AM (1 year, 27 days ago)

Hi Ilbiacco,

your Pioppino observations are very interesting, especially as you have a strain that grows on fermented straw. It should have a PH of ca. 5.5, so it's much easier for the mycelium.

There is this old story about Italians growing Pioppino large scale on fermented corn straw, which I never believed. Could you do a quick web search in Italian, to see if you can find any hints? The only reference I found so far came from stamets and could be pure fantasy, like the commercial cultivation of Parasols in Europe.

Carsten


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Re: Grain spawn without pressure cooking [Re: Alkaloids]
    #14398634 - 05/04/11 06:22 AM (1 year, 27 days ago)

Hi Alkaloids,

you are totally correct in avoiding plastic, as long as it gets produced from mineral oil! At least I can reuse those bags after fermentation.

Please keep us updated if you try the oil variant!

Carsten

PS: You may also look into the lactic acid fermentation of vegetables.


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OfflineILBIACCO
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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14403557 - 05/05/11 02:24 AM (1 year, 26 days ago)

Quote:

Mycelio said:
...There is this old story about Italians growing Pioppino large scale on fermented corn straw, which I never believed. Could you do a quick web search in Italian, to see if you can find any hints? The only reference I found so far came from stamets and could be pure fantasy, like the commercial cultivation of Parasols in Europe.

Carsten




Ciao Mycelio,
i find nothing about it. I think you're right. Probably it's only fantasy, but if i find something in future i will post it immediatly.

Parasol? Macrolepiota Procera? Cultivated for commercial purpose?
I've never heard anything concerning this.  But, why not? it could be an hot item, a real business.
Here in Italy it's a well-known and very appreciated mushroom.
One might think to produce mycelium beginning from stem butts on fermented straw...:cool:


--------------------
I've been waiting for her for so long
Open the sky and let her come down
Here comes the rain
Here she comes again
I love the rain
Rain Rain Rain
(The Cult)


Edited by ILBIACCO (05/05/11 02:54 AM)


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OfflineMycelio
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Re: Grain spawn without pressure cooking [Re: ILBIACCO]
    #14404121 - 05/05/11 07:17 AM (1 year, 26 days ago)

Thank you for searching!

And yes, I meant M. procera. There have been a few professional cultivation attempts decades ago, but I think fruiting was unreliable, while indoor fruits looked terrible. Even blocks, that have been buried outdoor, often won't produce.

Carsten


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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14439928 - 05/12/11 11:20 AM (1 year, 19 days ago)

hey mycelio  i was reading your tek and it sounds really really promising.  I got confused with all the comments on carbon sources and adding yeast.  what i was thinking of doing is following your tek  using straw for cubes.  I was gonna take a 5 gallon bucket fill it half way with straw cut into 3 inch pieces, fill wit water and just wait the 1-2 weeks as described stirring daily.  to aviod floaters i was  going to put netting over the straw and weight it down.
Do i need too cook the straw and if so how do i know when it is cooked enough?
will adding half a quart of cube mycelium spawned in wbs  work when added to the straw?
Any other advice or tweeks such as heat or that bulk mollasses  work to make the process go faster, and is it worth it?


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Re: Grain spawn without pressure cooking [Re: slssurvivor]
    #14440540 - 05/12/11 01:45 PM (1 year, 18 days ago)

Hey slssurvivor,

Quote:

slssurvivor said:
hey mycelio  i was reading your tek and it sounds really really promising.  I got confused with all the comments on carbon sources and adding yeast.  what i was thinking of doing is following your tek  using straw for cubes.  I was gonna take a 5 gallon bucket fill it half way with straw cut into 3 inch pieces, fill wit water and just wait the 1-2 weeks as described stirring daily.  to avoid floaters i was  going to put netting over the straw and weight it down.
Do i need too cook the straw and if so how do i know when it is cooked enough?



Just use pure, uncooked straw, but please start a small jar first, to test if your straw is fresh and clean enough. In case it had been wet before, it may not ferment on its own. If you see bubbles after some days, it is fine. Then you can safely start the bucked and pour in the jar as starter culture.
Keeping the straw below the surface is a good idea, people also use pillow sheets, onion bags and so on.
Try to minimize the surface, which is exposed to air.

Quote:

slssurvivor said:
will adding half a quart of cube mycelium spawned in wbs  work when added to the straw?



To the whole bucket? I would not go below 10% spawn (by weight).

Quote:

slssurvivor said:
Any other advice or tweeks such as heat or that bulk mollasses  work to make the process go faster, and is it worth it?



Cubes are not as acid tolerant as Pleurotus species, so avoid adding carbohydrates like sugar, starch, bran or grain. This would lead to a much stronger fermentation, ending up at PH 4 instead of 5.5.
A warm place helps, but heating is not worth the hassle.
Once, you're successful, you can try adding coffee grounds or alfalfa to increase nitrogen content without lowering the PH.

For me, the easiest way is fermenting pelletized straw in ziplock bags.

Carsten


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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14446590 - 05/13/11 03:58 PM (1 year, 17 days ago)

alright  thanks man  do the ziplock bags help keep the straw submerged?
I put a piece of tinfoil over the straw in the jar and a rock on top of that to keep it down, but any slight movement brings more pieces of straw to the top, so if i have to stir the jar everyday thats gonna be a problem :frown:  any suggestions?


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OfflineMycelio
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Re: Grain spawn without pressure cooking [Re: slssurvivor]
    #14446681 - 05/13/11 04:16 PM (1 year, 17 days ago)

If you can keep everything submerged, you don't have to stir. Just remove floating pieces.

When using ziplock bags you press out all the air, seal it and just wait. This way everything stays wet and without contact to fresh air.

Carsten


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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14463110 - 05/16/11 03:41 PM (1 year, 14 days ago)

Quoted from here:
http://www.shroomery.org/forums/showflat.php/Number/14461591#14461591

Quote:

MonkeyKnifeFight said:
I've seen mention of fermenting straw before but unclear on the specifics.  Do you just sink the straw in water for a couple weeks and then use it straight?  Or do still have to pasteurize it a the end of the process?  Is there any indication of whether the fermentation is complete?  Smell perhaps?




Yes, you need to keep the straw completely submerged. After ca. three days at 20°C it starts to smell sour and produces bubbles of CO2 for ca. 10 days. Then you only press out excess water and spawn as you like. There is no heating required and you can touch the straw with bare (clean) hands.

In case you still have those straw pellets, you can just put some into a ziplock freezer bag, add 1.5 - 2 times their weight in water, press out all air, forget it for two weeks and then use it without the need to press out water. In sealed bags there is little to no CO2 production, but you will notice the smell through the plastic.

The smell could be described as sour and sweet. Towards the end you will also notice vinegar.

Carsten


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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14498350 - 05/23/11 09:28 AM (1 year, 8 days ago)

A little update from my latest experiments...

This time I fermented the following mixture:
35% compressed sawdust bricks (beech)
35% straw pellets
15% coffee grounds
10% wheat
5% alfalfa pellets
I used 500g dry ingredients and 1070g water. The sawdust was mixed in after a week, as I didn't want to risk anything. In the end the fermented substrate had a slight alcoholic smell. Upon closer inspection I found a small hole in the bag. I filled everything into a new bag and covered the surface with 145g of oyster substrate spawn from last time. To speed up colonization, I made two fat holed into the block, so the mycelium won't have to grow such a long way through the substrate, but colonization time did not decrease much. Eleven days after inoculation there are only small uncolonized spots left at the corners. Within two or three days I will place the block on my balcony for fruiting. Mycelium growth was extremely dense and no mold showed up anywhere.

Compared to my usual procedure of pasteurizing substrates and thoroughly mixing with 50% substrate spawn, this takes more time for fermentation and colonization, but causes very little work and requires less spawn.

Carsten





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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14499289 - 05/23/11 01:11 PM (1 year, 7 days ago)

hey mycelio  where do you get your pelletized straw.  Im here in the east coast oft he states and cant find any pet or garden store that has it.  Anyone have any good brands that are ez to find?  also  is it possible to use rabbit pellets  for this?


thx


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Re: Grain spawn without pressure cooking [Re: slssurvivor]
    #14499745 - 05/23/11 02:49 PM (1 year, 7 days ago)

I hope somebody knows a local source. If nobody responds, check these places that sell everything around horses.

Carsten


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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14573468 - 06/07/11 08:34 AM (11 months, 17 days ago)

Mycelio,
you are the man!:thumbup:

Update

Finally, i have found my way for cultivating P.Ostreatus with fermented stuff.

-Step 1
I sink little pieces of oyster tissue, for one second, in a solution of hydrogen peroxide and tap water.
-2
I put these pieces of mushroom between two sheets of wet corrugated cardboard, in a large jar.
-3
After a few days, when the mushroom begins to grow through the cardboard, i add the fermented stuff.

I don't know why, but in this way, for the first time, i have obtained a vigorous growing, without any problems. Probably, my old oysters, bought in supermarket, need this process.
Greetings.


--------------------
I've been waiting for her for so long
Open the sky and let her come down
Here comes the rain
Here she comes again
I love the rain
Rain Rain Rain
(The Cult)


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Re: Grain spawn without pressure cooking [Re: ILBIACCO]
    #14574021 - 06/07/11 10:41 AM (11 months, 17 days ago)

Quote:

ILBIACCO said:
Mycelio,
you are the man!:thumbup:

Finally, i have found my way for cultivating P.Ostreatus with fermented stuff.
Greetings.




Cool!

Mycelio,
you ARE the man!!!


--------------------


"All the noobs jump on it like sliced bread has just been invented, and then. . . .nothing." RR


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OfflineMycelio
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Re: Grain spawn without pressure cooking [Re: aris]
    #14574372 - 06/07/11 12:02 PM (11 months, 16 days ago)

Thanks guys, though please do not put me on a too high podium.

@ILBIACCO
I'm happy it works for you too! Supermarket oysters are often several days old, being stored next to rotting fruit and vegetables, so weak growth in the first days and occasional contams do happen. Once you got strong growth, the mycelium is pretty much indestructible.

Carsten


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OfflineRogerRabbitM
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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14574512 - 06/07/11 12:34 PM (11 months, 16 days ago)

Quote:

Mycelio said:





I didn't scroll back to see what you're doing with it, but you are aware that's some serious bacteria infection aren't you?
RR


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Re: Grain spawn without pressure cooking [Re: RogerRabbit]
    #14576409 - 06/07/11 07:02 PM (11 months, 16 days ago)

Yes, you see lactic acid bacteria and yeasts acting like firefang in Agaricus compost. As you know, oyster mycelium tolerates the low PH and has them for lunch, while other contams can't get a foothold.

Carsten


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Re: Grain spawn without pressure cooking [Re: Mycelio]
    #14576787 - 06/07/11 08:17 PM (11 months, 16 days ago)

OK, just wondered what was going on.  Yes, Oysters thrive in low pH.  I find them in temperate rainforests where the natural pH of the alders they fruit on are around 4 to 5.
RR


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