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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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OfflineTheShroomHermit
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Registered: 02/19/02
Posts: 7,575
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Ps. Cyan without agar work?
    #1429724 - 04/04/03 06:25 PM (21 years, 17 days ago)

Bob asked me how Jane would go about cultivating from a wild Ps. Cyan print without working with agar.
Can you help me help him help her out?

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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 193,665
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Re: Ps. Cyan without agar work? [Re: TheShroomHermit]
    #1431295 - 04/05/03 12:25 PM (21 years, 16 days ago)

Tell Bob that janes project is doomed....always run wild prints on agar...

P#1


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California Has Legalized Child Prostitution

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OfflinePaid
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Registered: 03/12/03
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Re: Ps. Cyan without agar work? [Re: Prisoner#1]
    #1431333 - 04/05/03 12:44 PM (21 years, 16 days ago)

If you dont have agar to work with then i would use half the print on a
jar of grain or 2 and the other half in a honey water tec adding h2o2 after germination if there are any impuritys.Good luck with it.


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OfflineDatrutankdogg69
Cyan Man
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Loc: Pacific NW, Or.
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Re: Ps. Cyan without agar work? [Re: Paid]
    #1431430 - 04/05/03 01:17 PM (21 years, 16 days ago)

Well, Bob an Jane I know that when I picked a pound of them last year I dumped all the spore prints I didn't sale and all the sporewater from rinsing that pound onto a dumptruck full of woodchips that got dumped in mah yard. Well it's a year later an still not completley colonised(about 3/4 or more). So now I've gotta move an I'm stuck with a couple hundred pounds of spawn thats just getting bigger as we speak. So I hope to expand with alder and get some real fruits All that spawn an all I got was an oz or 2. ALOT of aborts an imature and some were growing into tha ground! I'm thinking this is because of the high sap content. I used mostly pine cause in was free.. but if I expand to alder.. AaahhhhIcan'twait...

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InvisibleOlgualion
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Re: Ps. Cyan without agar work? [Re: Paid]
    #1431560 - 04/05/03 02:12 PM (21 years, 16 days ago)

The only problem with that is:

In the time that the cyan spores germinate in the H.W. so will any contams. AFAIK - H2O2 will only kill ungerminated spores.

I have seen practically sterile prints made from wild specimens. This is about the only chance you would have.


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Study the past...
See the future...

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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Ps. Cyan without agar work? [Re: TheShroomHermit]
    #1431571 - 04/05/03 02:22 PM (21 years, 16 days ago)

Wild prints always have a higher risk of being contaminated , so doing agar work is beneficial to issolating a "clean" isolation .
Agar work is not a scary as some may make it sound , just requires being as clean as humanly possible , patience , agar and petri dishes .
Good Luck
PS If you try agar , hravest spores from the center of the print , they are more likely to be contam free .


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !

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Offlinecurenado
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Registered: 04/01/03
Posts: 2,603
Loc: North Central Arkansas
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Re: Ps. Cyan without agar work? [Re: tripndicular]
    #1432636 - 04/05/03 10:47 PM (21 years, 15 days ago)

I keep looking out for some wild. Got a buddy been ripped off twice trying to buy a sample. If one gets fresh pure wild culture one could end up with a really strong strain. Bravo!


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Yours in the Natural State Land of Enchantment!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."

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Offlinecurenado
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Re: Ps. Cyan without agar work? [Re: curenado]
    #1441584 - 04/09/03 01:26 AM (21 years, 12 days ago)

Paid -
<<...the other half in a honey water tec adding h2o2 after germination if there are any impurity>>
You can add H202 to liqui-germ? I use the karo mix, but didn't know you could add H202 after germ to clean? How much?  When one of those liquid jars goes cruddy - oh are they ugly and scary looking! :shocked:


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Yours in the Natural State Land of Enchantment!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."

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OfflineoO_wombat_Oo
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Registered: 06/04/01
Posts: 812
Loc: NSW, Australia.
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Re: Ps. Cyan without agar work? [Re: TheShroomHermit]
    #1441662 - 04/09/03 02:00 AM (21 years, 12 days ago)

I was actually wondering the same thing concerning some Wild Cubensis prints that I have. I don't have agar or petri and can't be f'd using it. Isn't there another way?

What are my chances if I just pretended the prints were sterile? None?

I also have a wild Copelandia Cyanescens print. Screwed if I know what I'm going to do with it though. I could't grow Copes even if the print was sterile. :smile:

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Invisibledeanofmean
mycophagous

Registered: 12/06/02
Posts: 2,017
Loc: PNW
Re: Ps. Cyan without agar work? [Re: curenado]
    #1441706 - 04/09/03 02:16 AM (21 years, 12 days ago)

germinate spores on cardboard .
what i do is, soak a 1/4" square of egg carton, put it in a jar and pc it .
dab the cardboard on the spore print to pick up some spores, and put it back in the jar . after spores germinate drop it into karo water with h2o2 .
i've found when you use h2o2 in liquid culture, mycelium does'nt grow like a cloud .
instead you will see little white specks floating around, and sometimes
will look like smoke .
don't worry it is healthy mycelium .

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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