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InvisibleShot.In.Orbit
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Liquid Culture Recipe - Does this sound about right to you?
    #13617253 - 12/11/10 01:02 PM (13 years, 3 months ago)

About to make a LC media. Just want to double check with everyone here before I make it. Here is the recipe I will be using.

10g Dextrose
10g Light Malt Extract.
800ml Distilled Water.


Does this sound good to the rest of you?

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OfflineMycoAu
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13617282 - 12/11/10 01:12 PM (13 years, 3 months ago)

Looks fine.  Typically you want your sugar sources to total about 2-4% of the final weight/volume.  Yours is about 2.5%.

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13617604 - 12/11/10 02:29 PM (13 years, 3 months ago)



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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13617730 - 12/11/10 02:56 PM (13 years, 3 months ago)

Quote:

Shot.In.Orbit said:
About to make a LC media. Just want to double check with everyone here before I make it. Here is the recipe I will be using.

10g Dextrose
10g Light Malt Extract.
800ml Distilled Water.


Does this sound good to the rest of you?




I do by volume. 1 tablespoon of each per 1 quart/1 L
Filtering (a few times) as I load it into LC jars + stirbar
PC with lids loose for 20-25 minutes with a prior 5 minute "off-steam"
PC cools, tighten lids.  Let cool for 24hr-ish


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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: dmonkey1]
    #13618093 - 12/11/10 05:00 PM (13 years, 3 months ago)

Quote:

dmonkey1 said:
I do by volume. 1 tablespoon of each per 1 quart/1 L
Filtering (a few times) as I load it into LC jars + stirbar
PC with lids loose for 20-25 minutes with a prior 5 minute "off-steam"
PC cools, tighten lids.  Let cool for 24hr-ish





Yeah I was reading that "By Volume" And I converted a recipe I found here on the board Agar put on (I believe it was agar) about a good mix for LME/Dextrose LC and he wrote out the same you did...
1tbsp Light Male Extract
1tbsp Dextrose
1 Quart water

I am just not so certainly familiar with Quarts and Tablespoons ect... I prefer the metric system for clear amounts and accuracy. So I weight out 1 table spoon and rounded to a even Number and got 10g. for both LME and Dextrose... then poured out 1 quart of water and got 720g or rounded liquid measurement 800ml. or 760g of water

that is where is got the recipe:

10g Light Malt Extract
10g Dextrose
800ml Distilled Water

I was am just trying to confirm that this was a accurate representation of the 1 tbsp lme 1 tbsp dex. 1 quart water.

one quart would be 800ml roughly right?... Maybe I should use 1000ml or 1 liter of Water to dilute it a bit more. so It is not so strong I have read less is more when it comes to LC

Edited by Shot.In.Orbit (12/11/10 05:10 PM)

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: MycoAu]
    #13618126 - 12/11/10 05:06 PM (13 years, 3 months ago)

Quote:

MycoAu said:
Looks fine.  Typically you want your sugar sources to total about 2-4% of the final weight/volume.  Yours is about 2.5%.





Hey man... your the shit. Thanks for converting my concentration level for me... This is what I was most concerned with.

So just to make sure I understand what your telling me...           
I have approximately 2.5% dissolved sugars in my Media per volume of water. This being the Light Malt Extract and Dextrose.

And the Ideal amount of dissolved sugars wanted is between 2% and 4% the volume of water.

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13618210 - 12/11/10 05:20 PM (13 years, 3 months ago)

or you could just make agar broth
is agar diluted 1/4th the regualr recipe
im meaning to wright a tek

it works amazing trust me
the little bit of money you spend doing it
is far worth it

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: jokefox]
    #13618259 - 12/11/10 05:33 PM (13 years, 3 months ago)

Hmmm. I tell you what buddy.

You write the TEK and I will give it a try. I have all the materials for agar. MEA Agar and PDYA Agar... Drop me a PM when you make it and I will try it and post results giving you credit and proving your TEK for others to try....

Sound like a interesting way of making LC Media.


*Ding* just finnished brewing my LME/Dextrose LC Media... now time to PC :smile:

Edited by Shot.In.Orbit (12/11/10 05:35 PM)

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InvisibleBase Icks
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619514 - 12/12/10 01:24 AM (13 years, 3 months ago)

Quote:

Shot.In.Orbit said:
liquid measurement 800ml. or 760g of water




:dudewtf:

1ML of water is 1g.

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Invisibleslapphappypill
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Base Icks]
    #13619559 - 12/12/10 01:39 AM (13 years, 3 months ago)

lol, a few of those conversions went over my head as well...

[threadjack]I love the new update to your avatar bro, sexy ass hippie chicks! wurd![/threadjack]


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I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Base Icks]
    #13619588 - 12/12/10 01:49 AM (13 years, 3 months ago)

Quote:

Base Icks said:
Quote:

Shot.In.Orbit said:
liquid measurement 800ml. or 760g of water




:dudewtf:

1ML of water is 1g.




I got a shit digital scale :eek:

lol really I poured 800ml and it read 760... thinking it was good... guess I was wrong.

LOL RIGHT? WTF...
(everyone point and laugh at me)
:facepalm3:

(Cool avatar BTW)

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619612 - 12/12/10 01:57 AM (13 years, 3 months ago)

Thanks.

I use a syringe to calibrate my scales. Or a coin that I know the weight of. UK pennys are 3.5g, and demonination of dollar bills are 1g.

Put empty syringe on scale, zero(assuming its digital) add x ml of h2o, scale should read xg.

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: slapphappypill]
    #13619614 - 12/12/10 01:57 AM (13 years, 3 months ago)

Quote:

slapphappypill said:
[threadjack]I love the new update to your avatar bro, sexy ass hippie chicks! wurd![/threadjack]




Jack all you want I don't think anyone cares about this thread anymore... I was just trying to confirm what i was about to mix was good.


That Avatar picture would be look hilarious if that mushroom was a Penis Envy strain...  :yesnod:

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Base Icks]
    #13619619 - 12/12/10 01:59 AM (13 years, 3 months ago)

Quote:

Base Icks said:
Thanks.

I use a syringe to calibrate my scales. Or a coin that I know the weight of. UK pennys are 3.5g, and demonination of dollar bills are 1g.

Put empty syringe on scale, zero(assuming its digital) add x ml of h2o, scale should read xg.





Sounds like I should get on that... because I just mixed a batch of LC and the recipe i just mixed could be all screwed up

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619624 - 12/12/10 02:00 AM (13 years, 3 months ago)

:bigyesnod:

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619629 - 12/12/10 02:01 AM (13 years, 3 months ago)

WHY THE F... DO I QUOTE EVERYTHING SOMEONE SAYS?  :um:

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619644 - 12/12/10 02:04 AM (13 years, 3 months ago)

Quote:

Shot.In.Orbit said:
WHY THE F... DO I QUOTE EVERYTHING SOMEONE SAYS?  :um:



Cause your a noob. I only do it if its a few post up, or its really important, but mostly if I want to be really annoying or make fun of someone.

:tard:

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619653 - 12/12/10 02:07 AM (13 years, 3 months ago)

Hmmm thats... I dont know?

I tried 1ml, 5ml, and 10ml and the scale said 1g, 5g, 10g... Maybe the scale starts to become less accurate the heavier of an object thats placed on it.

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Base Icks]
    #13619659 - 12/12/10 02:09 AM (13 years, 3 months ago)

Quote:

Base Icks said:
Cause your a noob.





Yeah I'll Quote that.  :awethumb:

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619671 - 12/12/10 02:13 AM (13 years, 3 months ago)

Use a measuring cup or something then? When I do that scale still is within 5%.

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Base Icks]
    #13619685 - 12/12/10 02:17 AM (13 years, 3 months ago)

I am really not worried about the scale anymore after the test you just had me do. I have measuring cups scaled in ml... so I really have no need to measure anything heavy. like water I have just been using it for 20g and below measurements like LME Dextrose Agar-Agar and the lot... Now I know its good for the small measurements I am sure that batch of LC I made earlier is all good.

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619701 - 12/12/10 02:21 AM (13 years, 3 months ago)

:billymaythumbup:

2-4% sugar content for lc and agar. I prefer 2% on agar for faster colonisation.
4% for lc, for stronger lc. Thats just me though.

I also do it by weight, not volume.

:shroomeryhead:

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Base Icks]
    #13619734 - 12/12/10 02:32 AM (13 years, 3 months ago)

Thats just good info. I am going to remember that for sure! :thumbup:

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Base Icks]
    #13619814 - 12/12/10 03:05 AM (13 years, 3 months ago)

Quote:

Base Icks said:

4% for lc, for stronger lc. Thats just me though.



:shroomeryhead:




whats 4% in 1:1 lme/dex, in american tablespoons?  :ameridillo:


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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: dmonkey1]
    #13619820 - 12/12/10 03:06 AM (13 years, 3 months ago)

:shrug:

I only work with weights.

And a tablespoon is a tablespoon.

I'm american btw.

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: MycoAu]
    #13619879 - 12/12/10 03:27 AM (13 years, 3 months ago)

Quote:

dmonkey1 said:
whats 4% in 1:1 lme/dex, in american tablespoons?




I'll take a crack at that...

1 tbsp = 10g Dextrose
1 tbsp = 10g LME

And I have a 2.5% mix say's MycoAu

Quote:

MycoAu said:
Looks fine.  Typically you want your sugar sources to total about 2-4% of the final weight/volume.  Yours is about 2.5%.




So based on that... You would do some math.
(here I will do it for you... because I am awesome.)

2.5 - 4 = 1.5 so add that to 10g and you get 15g

-----------------------------------------------------------------------

So you would want to mix this...

15g - Light Malt Extract
15g - Dextrose
800ml - Distilled Water

and you should have a 4% Liquid Culture Media. (provided that MycoAu was correct saying I have a 2.5% mix)


Did that help?

Edited by Shot.In.Orbit (12/12/10 03:29 AM)

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619891 - 12/12/10 03:33 AM (13 years, 3 months ago)

two tablespoons it is.

Jw, because i've been having fucking bacterial contams RIGHT at the end.  Maybe the high sugar content will make the myc stronger ?  I've only heard good things about 2:2 lme/dex, now I know its ~4% :smile:


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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: dmonkey1]
    #13619892 - 12/12/10 03:33 AM (13 years, 3 months ago)

Quote:

dmonkey1 said:whats 4% in 1:1 lme/dex, in american tablespoons?





Wow... I got all excited thinking I am fucking smart... I didn't even answer your question... so here is your answer...

If:
10g = 1 tbsp
then:
15g = 1.5 tbsp

so you would mix this

1 1/2 tbsp - Light Malt Extract
1 1/2 tbsp - Dextrose
in 800ml water or 1 quart (approximately)

Okay I think I may have helped... this time... :tongue2:

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619894 - 12/12/10 03:34 AM (13 years, 3 months ago)

haha it's all good i think 2:2 with 1 quart/1000mL will be 4%


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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: dmonkey1]
    #13619906 - 12/12/10 03:37 AM (13 years, 3 months ago)

Quote:

dmonkey1 said:
two tablespoons it is.




Negative Ghost Rider...

If what MycoAu was correct in me have 2.5% media then you adding 2:2 you mean you would have a 5% solution...

you need to add 1.5:1.5 per 800ml water... to have 4%

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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619907 - 12/12/10 03:38 AM (13 years, 3 months ago)

Ohh your going 1000ml of water... okay then yeah that would be pretty close to 4% then...

I was basing this off my mix using 800ml of water...


okay... okay... you right then 2:2 it is

1 quart is 1000ml ? (see I dont know standard american measurements I always use metric so i dont know really what a quart is...)

Edited by Shot.In.Orbit (12/12/10 03:40 AM)

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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Shot.In.Orbit]
    #13619914 - 12/12/10 03:40 AM (13 years, 3 months ago)

:awebig:


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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: dmonkey1]
    #13619965 - 12/12/10 04:00 AM (13 years, 3 months ago)


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InvisibleShot.In.Orbit
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Re: Liquid Culture Recipe - Does this sound about right to you? [Re: Base Icks]
    #13620064 - 12/12/10 04:54 AM (13 years, 3 months ago)

1 US quart = 946.352946 millilitres

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