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Offlineyaguaretr
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Registered: 03/21/10
Posts: 4
Last seen: 12 years, 8 months
Lions mane slant question
    #13441324 - 11/05/10 10:40 PM (13 years, 4 months ago)

Hi,

I've been reading these forums for great info for a while now but never posted.

I was hoping someone could help me out with a question i have.
I obtained a lions mane strain on agar plate lately and made some more plates/slants from the plate.

I made the slants in the usual way, agar ME mix with a sliver of wood in it.

All seems good no contams. Left the slant in the incubator for a week the noticed the lionsmane seems to be growing more than just myc in the slant. Ive never grown lions mane before but it looks like its fruiting in the test tube.
I Am bit concerned that now moving the slant to the fridge the fruit will die in the slant and could cause possible contam issues.

I was wondering if anyone has any experience with lions mane slants and could offer advice. I have included photos. Should i redo some slants with less air and absolutley no light available to the slants??
i keep me incubator failry dark but iits not completly dark.


thanks

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OfflineeVenom
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Registered: 08/25/07
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Re: Lions mane slant question [Re: yaguaretr]
    #13441439 - 11/05/10 11:08 PM (13 years, 4 months ago)

mine is got brown after 1 year but it still revived and performed as before but I haven't seen mine fruit in the slant!

do you use paraflim?

what tubes do you use?

do you have any pic's?


--------------------
eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR

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Offlineyaguaretr
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Registered: 03/21/10
Posts: 4
Last seen: 12 years, 8 months
Re: Lions mane slant question [Re: eVenom]
    #13441894 - 11/06/10 01:35 AM (13 years, 4 months ago)

sorry i tried to post a pic but it didnt work.
here is the link:

http://img248.imagevenue.com/img.php?image=17770_lionsmaneslants_122_412lo.jpg

I dont have any parafilm i just back off the screw lid and wrap a bit of micropore tape around the top.


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Offlinefungus_tao
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Re: Lions mane slant question [Re: yaguaretr]
    #13442349 - 11/06/10 07:04 AM (13 years, 4 months ago)

Lion's mane is notorious for pinning on agar and fruiting early. It will start to fruit on grain before the grains are even finished colonizing. My advice is to redo the slants. Next time try to get a better angle when you set your slants to maximize surface area, and don't incubate them. Give them 3-5 days at normal room temps, then they can go in the fridge for long term storage.


--------------------
Follow the light
The Light is your guide.

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OfflineRogerRabbitM
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Registered: 03/26/03
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Loc: Seattle
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Re: Lions mane slant question [Re: yaguaretr]
    #13442624 - 11/06/10 09:19 AM (13 years, 4 months ago)

They're fine.  Later, when you use the slant to inoculate a petri dish, you can grab the agar or a part of the fruit that's growing.
RR


--------------------
Download Let's Grow Mushrooms



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OfflineBuckeye Oysters
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Registered: 08/09/08
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Re: Lions mane slant question [Re: RogerRabbit]
    #13444501 - 11/06/10 05:11 PM (13 years, 4 months ago)

RR is right.  Just dont let slant over incubate and completly fruit in vitro otherwise it is possible to bruise fruiting mycellium and strike up contam when making transfers.


--------------------
Evolution is Lamarckism in disguise.  Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated.  For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.

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InvisiblePeterthinks
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Registered: 11/10/04
Posts: 2,379
Re: Lions mane slant question [Re: yaguaretr]
    #13445301 - 11/06/10 07:59 PM (13 years, 4 months ago)

Quote:

yaguaretr said:







CUTHULU!!!


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
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Offlineyaguaretr
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Registered: 03/21/10
Posts: 4
Last seen: 12 years, 8 months
Re: Lions mane slant question [Re: Buckeye Oysters]
    #13446935 - 11/07/10 04:42 AM (13 years, 4 months ago)

sweet, thx for the feed back.
have moved them to the fridge.


thx again

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