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Offlinedr0384
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Registered: 07/17/10
Posts: 391
Loc: Australia
Last seen: 6 months, 9 days
peroxide agar tek bunk?
    #13122480 - 08/30/10 04:57 AM (13 years, 6 months ago)

is this tek outdated? Is is any good?

http://www.shroomery.org/forums/showflat.php/Number/466150#466150

Its adding peroxide to agar to make it more tolerant to bacteria and spores?


--------------------
Good judgement comes from experience.
Experience comes from bad judgement.

Be yourself whoever that is because
those who care don't matter
and those who matter wont care.




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Offlinedr0384
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Registered: 07/17/10
Posts: 391
Loc: Australia
Last seen: 6 months, 9 days
Re: peroxide agar tek bunk? [Re: dr0384]
    #13122523 - 08/30/10 05:30 AM (13 years, 6 months ago)

I've been told in previous thread that it's bunk. Wanted clarification.


--------------------
Good judgement comes from experience.
Experience comes from bad judgement.

Be yourself whoever that is because
those who care don't matter
and those who matter wont care.




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OfflineNikon
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Registered: 01/29/09
Posts: 147
Last seen: 12 years, 6 months
Re: peroxide agar tek bunk? [Re: dr0384]
    #13122580 - 08/30/10 06:13 AM (13 years, 6 months ago)

I've used it and found it works. Growth can be stunted though depending on how much you use. If bacteria/mold is giving you woes, give it a shot.

Only thing is you can't grow from spores with peroxide agar dishes, only from tissue and myc cultures.

I'm interested to see what those people who think it's bunk say.

I germinate wild spores on normal agar and then transfer sectors to very slightly peroxided agar. I find it good to keep contams at bay and cultures very clean. Slower growth though. Also i'm much more confident i'm going to have clean LC's when transferring agar wedges from the peroxided plates also.

Edited by Nikon (08/30/10 06:23 AM)

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InvisibleDoc_T
Random Dude
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: peroxide agar tek bunk? [Re: dr0384]
    #13122611 - 08/30/10 06:35 AM (13 years, 6 months ago)

Quote:

dr0384 said:
is this tek outdated? Is is any good?




It's not necessary. Good technique allows you to do all you need without additional chemicals.


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You make it all possible. Doesn't it feel good?

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