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OfflineBaby_Hitler
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Do Azures require agar?
    #1298039 - 02/10/03 10:34 PM (11 years, 5 months ago)

I'd like to do some Azures. Will I have to grow out mycelium in a petri dish? Somebody told me in another post that you could germinate spores on cardboard. I didn't realize this was possible.

Is it?

Somebody point me towards a good tek.  :smile:


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InvisibleG a n j a
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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1298049 - 02/10/03 10:42 PM (11 years, 5 months ago)

I dont think you have to start azures on agar.
But if you do i'm about to try cardboard squares on pda + peroxide agar.I think the idear is that the spores germinate on the cardboard(were other spores find it hard to germinate)then the mycelium moves onto the pda + P agar which helps keep contams down.
You can also use cardboard and beer theres a thread here somewere about it.


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Invisiblemobyd
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Re: Do Azures require agar? [Re: G a n j a]
    #1298572 - 02/11/03 05:45 AM (11 years, 5 months ago)

Hi!

I started to isolate spores on Agar. Then transferred onto rye (mixed with some wood chips and cardboard) and then onto wood/or cardboard.

Forget to put agarpieces onto cardboard it won?t work. That?s my opinion.

mobyd


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Anonymous

Re: Do Azures require agar? [Re: Baby_Hitler]
    #1298738 - 02/11/03 06:55 AM (11 years, 5 months ago)

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Offlinecomario2
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Re: Do Azures require agar? [Re: Anonymous]
    #1298772 - 02/11/03 07:17 AM (11 years, 5 months ago)

yes. as per these pics


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comario


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OfflineBaby_Hitler
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Re: Do Azures require agar? [Re: comario2]
    #1298938 - 02/11/03 08:21 AM (11 years, 5 months ago)

"Yes" you need agar, or "Yes" you can germinate spores on cardboard?


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Offlinecomario2
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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1298955 - 02/11/03 08:26 AM (11 years, 5 months ago)

yes to both. the pic i posted is azure myc germinated on MEA. the one posted here below shows spores germination on cardboard placed on a PEROXIDATED agar plate


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comario


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Edited by comario2 (02/11/03 09:23 AM)


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Offlinecomario2
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Re: Do Azures require agar? [Re: comario2]
    #1298970 - 02/11/03 08:31 AM (11 years, 5 months ago)

here is the same petri from the previous post, after the spores have germinated on the cardboard (slowly) and then grown over the - PEROXIDATED - agar (aggressively)
  note that this tek is used to benefit from low contam rate of cardboard (as opposed to non perox MEA) for spores germination. yes, the cardboard i used with success is the thick gray cbd (from the back of note pads) :smile:


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comario


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Edited by comario2 (02/11/03 09:20 AM)


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Invisibledeanofmean
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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1299086 - 02/11/03 09:03 AM (11 years, 5 months ago)

mycelium seems to prefer gray cardboard, like the backing of a notepad .not sure what the difference is .egg cartons work good too .


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OfflineBaby_Hitler
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Re: Do Azures require agar? [Re: comario2]
    #1299693 - 02/11/03 11:54 AM (11 years, 5 months ago)

Do you still have to use a flow hood, or glove box when using cardboard, and peroxidated agar?

Do you have to PC anything?


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OfflineRaadt
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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1299740 - 02/11/03 12:11 PM (11 years, 5 months ago)

I use peroxidated agar and never have to use a flow hood, or glove box. I just clean up where i'm working.

The process for making peroxidated agar is the same as any agar - mix, and pc - but in the end, you add some peroxide prior to pouring, I use 4cc's in a 500ml mixture.

You will not be able to germinate spores on this agar, but as far as cloning and stuff go, it's great!


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Re: Do Azures require agar? [Re: Raadt]
    #1300003 - 02/11/03 01:42 PM (11 years, 5 months ago)

Do you use the standard 3% H2O2?

Could I buy a jar of presterilized agar, and add peroxide to it?

I won't be using it all the first time, will it keep? Will it have to be refrigerated?

So I pour the dishes, and let them set, then take some cardboard soaked in boiled beer, and stick them in the dish, then streak some spores onto the cardboard?

I think I've almost, but not quite, got it.


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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1300045 - 02/11/03 02:10 PM (11 years, 5 months ago)

great idea


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Edited by the spiral (02/11/03 02:13 PM)


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OfflineAnnoA
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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1300186 - 02/11/03 03:14 PM (11 years, 5 months ago)

I?ve been using a different method on various occasions, and it worked well.

I make a fairly diluted spore suspension and inoculate a classic PF cake with it on 4 spots with little inoculant.

One or more of the spots usually show clean growth, then you can transfer those spots to either peroxided agar, or directly to peroxided grain.


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Re: Do Azures require agar? [Re: the spiral]
    #1300211 - 02/11/03 03:25 PM (11 years, 5 months ago)



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OfflineBaby_Hitler
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Re: Do Azures require agar? [Re: Anno]
    #1300216 - 02/11/03 03:28 PM (11 years, 5 months ago)

Hey, that's pretty smooth!  :smile:


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OfflineRaadt
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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1300237 - 02/11/03 03:36 PM (11 years, 5 months ago)

If you buy pre-made agar, yes you can just liquify it in a water bath, then add the peroxide. And if you want to keep the agar for later, you can just recover, and reliquify when ready for more use (you will need to add more peroxide at that point, and your mixture might become a little bit watery (some water on the top of the media) but it should work, even after the 1st, and if not - then you can always just try and be as clean as possible, as you would w/o peroxidated agar. It's not that easy to make a mess of, if you're careful.


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Re: Do Azures require agar? [Re: Raadt]
    #1300261 - 02/11/03 03:45 PM (11 years, 5 months ago)

Once I've got the mycelium going, will I have to transfer it to grain, or can I make a big batch of colonized cardboard, and spawn wood chips with that?


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OfflineRaadt
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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1300523 - 02/11/03 06:08 PM (11 years, 5 months ago)

I would imagine you could go to cardboard, and from there to chips. I believe stamets talks about cyan bed prep that way in one of his books. Cardboard spawning chips, long sheet of colonized cardboard with chips on top.


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Offlinecomario2
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Re: Do Azures require agar? [Re: Baby_Hitler]
    #1301559 - 02/12/03 05:54 AM (11 years, 5 months ago)

it would certainly help (to have a flow hood or glove box) but its not absolutely necessary. pc would be necessary for sterilizing grains to be used as spawn


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Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood, Malt Extract, Paul Stamets, Petri Dish, Potato Dextrose, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Outdoor Cultivation

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