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don't use beer, it only increases oppotunities for contams. simply put the cbd in water & microwave for three mins. when cool, place the dot of cbd on the peroxidated agar plate, then streak the cbd with spores
By far the easiest way to grow Azurescens without agar is by spawning wood chips with colonised chips. Its just a matter of adding the colonised chips to moist alder or hardwoods and watching it colonise. Contaminants are not an issue because generally the wood is selective. From spores i would suggest agar, but if not, try using a pftek type technique. Make a syringe from the print and innoculate either jars of just striaht woodchips, or a mix of chips, verm and brf to speed things up. If any of the points germinate and look clean, transfer to peroxidated agar. Again i will raise the point that just because you are using h202 agar, your still defeating the purpose of sterility if you expose the plates to open air. Contaminant spores will fall on the agar plate and although many may be killed by the h202, some will not and will very much enjoy the conditions when you transfer a wedge to your substrates. This isnt much of problem with woodlovers, but for mushrooms that require high nutrient substrates contamination is inevitable.