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 benitoamanito
rad
Registered: 06/18/05
Posts: 827
Last seen: 9 months, 30 days
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 an idea
#12965242 - 07/28/10 02:13 AM (1 year, 9 months ago) |
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scrap the orginal idea..
here is what im considering.
instead of using pressure cooking devices and high heat scenarios to sterilize grains or brf cakes, how about using a UV light method or even just using the suns natural UV to sterilize?
my reason to suggest this, is that ive noticed the high heat of pressure cooking gives way to more room for bacteria and mold to grow, as well as destroying beneficial bacterias that would help the mushroom spores to grow..
any thing that would be beneficial to spore growth, my guess would be resistant to UV ..
Edited by benitoamanito (07/28/10 03:24 AM)
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Doc_T
Random Dude
 
Registered: 03/06/09
Posts: 37,002
Loc: Colorado 
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No, this won't work.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 37,004
Loc: USA Mountain Northwest
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Re: an idea [Re: Doc_T]
#12965440 - 07/28/10 05:31 AM (1 year, 9 months ago) |
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Quote:
instead of using pressure cooking devices and high heat scenarios to sterilize grains or brf cakes, how about using a UV light method or even just using the suns natural UV to sterilize?
By that reasoning, there would be no bacteria or molds in nature where the sun shines. The UV light in sunlight does not kill molds. I've left trich infested substrates out in the sun for weeks and it didn't harm the mold at all.
We sterilize with steam because it works.
RR
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semper in excretia sumus solim profundum variat
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rave420
open minded
Registered: 07/20/10
Posts: 694
Loc: Vancouver Island
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uv light does not penetrate the substrate the same way that a good presure cook would. you might kill everything on the outside using UV light, but on the inside, your grain, verm, substrate whatever could still habor contaminants.
UV is great for sterilizing non-porous non absorbing surfaces or liquids, but it's virtually uneffective for anything that can contain bacteria and spores on the "inside".
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we all breathe the same air, drink the same water, and draw our strength from the same giant fireball.
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benitoamanito
rad
Registered: 06/18/05
Posts: 827
Last seen: 9 months, 30 days
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well how bout sun drying the grains then..
before inoculation that is
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 37,002
Loc: Colorado
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Dry enough to prevent spoilage? Like beef jerky?
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stoliohranj
Stranger
Registered: 11/28/05
Posts: 13
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Re: an idea [Re: Doc_T]
#12970652 - 07/29/10 06:00 AM (1 year, 9 months ago) |
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From what I understand, sunlight will inhibit the growth of most molds/bacteria that we are concerned with. But sunlight alone won't kill all the non-germinating spores. When you are sun drying your grains it is a hostile environment for the spores, but not necessarily a lethal environment. As soon as you take those grains and put them in a dark, moist environment (without a proper sterilization) they are more than happy to germinate and ruin your project.
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rave420
open minded
Registered: 07/20/10
Posts: 694
Loc: Vancouver Island
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i don't think you understand.
The sunlight does not get to the INSIDE of your grain, therefore spores will survive. I will give you a 100% money back guarantee that your grains will contaminate unless properly sterilized. Sunlight will NOT do. They need to be sterilized inside a jar that remains sterile after the sterilization process is done.
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we all breathe the same air, drink the same water, and draw our strength from the same giant fireball.
Help me with my collection (trades only)
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 EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 9,507
Loc: Canada 
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Offtopic but does anyone have any info on those UV Light wands? I saw one that claimed to kill 99.9 percent of bacteria and molds at a science store for 40 dollars canadian. Your supposed to expose said surface to 45 seconds of uv from this wand. My question is could you use this device to sterilize empty petri dish's, vacutainers and things of those nature? If so I think this would be a HUGE money saver. You could reuse some dish's instead of buying countless sleeves.
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Masticore
Perpetually Curious
Registered: 12/16/09
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Last seen: 1 day, 21 hours
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My advice would be to use UV light in addition to, and not in place of, regularly accepted sterile procedure. That way, even if the light does nothing, you're not hurting your chances. If the light helps though, you may kill off a few spores that would have killed your grow otherwise.
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Treat my posts like the Bible. I am fallible, subject to human limitation, and usually open to interpretation.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 37,002
Loc: Colorado
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UV scares the hell out of me. It causes cataracts, skin cancer, mutations.
I'd just as soon have a gamma ray source in my home.
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Fungal growth
Wee Todd
Registered: 03/21/10
Posts: 2,102
Loc: under a rock in your yard
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Quote:
EvilMushroom666 said:
Offtopic but does anyone have any info on those UV Light wands? I saw one that claimed to kill 99.9 percent of bacteria and molds at a science store for 40 dollars canadian. Your supposed to expose said surface to 45 seconds of uv from this wand. My question is could you use this device to sterilize empty petri dish's, vacutainers and things of those nature? If so I think this would be a HUGE money saver. You could reuse some dish's instead of buying countless sleeves.
the cancer you WILL get if you fuck up and expose yourself to concentrated UV light
will be much more expensive.
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 9,507
Loc: Canada
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What if you built a jig for it, which would set the UV field inside of a wooden box lets say? Its a as seen on t.v UV wand so im assuming its not SUPER powerful, is worth some more research perhaps.
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rave420
open minded
Registered: 07/20/10
Posts: 694
Loc: Vancouver Island
Last seen: 1 year, 6 months
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again, UV lights sterilize SURFACES and liquids that are premeable to the UV rays. grains are not. Grains harbor contaminants on the INSIDE, therefore surface sterilization will not be complete
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we all breathe the same air, drink the same water, and draw our strength from the same giant fireball.
Help me with my collection (trades only)
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 37,002
Loc: Colorado
Last seen: 10 minutes, 3 seconds
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Quote:
EvilMushroom666 said:
im assuming its not SUPER powerful, is worth some more research perhaps.
If it is safe to use, it is too weak to use.
UV has no place in home mycology.
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conformist
Registered: 07/29/10
Posts: 209
Loc: SE
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Barbers use breadbox sized UV enclosures to sterilize scissors and combs between customers. I believe the light cuts off when the door is opened, but I've seen some without doors.
Irradiation could be used to sterilize without heat. Not sure if it can be done in the home.
Similar but off topic: Would be cool if contams could be killed on cultures with something very focused like a laser (maybe a magnifying glass would work).
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 9,507
Loc: Canada
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Re: an idea [Re: Doc_T]
#12972407 - 07/29/10 01:47 PM (1 year, 9 months ago) |
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Twas an idea but I guess Ill leave it at that, a pipedream Id rather buy a box of 500 petri`s then deal with cancer.
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