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 mukhail
Creeper
Registered: 02/06/10
Posts: 868
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 PF cakes and isolates
#12970822 - 07/29/10 07:36 AM (1 year, 6 months ago) |
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So I just got an agar set from sporeworks, and I was wondering something...
Does anyone use isolates to knock up brf cakes? What kind of jar lids do you use if you do, and is it really a bigger advantage over multispore? Does anyone make liquid culture from isolates and just use classic pf cakes with the verm filter?
Inb4 start using grain, I understand the advantages of both and prefer pf cakes, Please dont try to convince me, I fully understand it.
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Doc_T
Random Dude
 
Registered: 03/06/09
Posts: 34,902
Loc: Colorado 
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Re: PF cakes and isolates [Re: mukhail]
#12970837 - 07/29/10 07:47 AM (1 year, 6 months ago) |
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I'm doing clones on agar -> lc for cakes. Works great.
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 hamloaf
Hamington Loafberg
Registered: 12/23/09
Posts: 6,063
Loc: The Electric Guitar.
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Re: PF cakes and isolates [Re: mukhail]
#12970839 - 07/29/10 07:48 AM (1 year, 6 months ago) |
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Use grain jar or LC lids on your BRF jars if your are going to inoculate BRF cakes with agar isolates, no verm barrier. Number 1 advantage of inoculating BRF cakes with agar is increased colonization speed. This will help defend against contaminates as well. Plus with out the verm layer, more substrate can be placed into your jars.
HL
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mukhail
Creeper
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Re: PF cakes and isolates [Re: hamloaf]
#12970891 - 07/29/10 08:12 AM (1 year, 6 months ago) |
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Excellent!
Ill have to start making silicon injection port jar lids then.
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phaseflux
Stranger than most
Registered: 07/16/08
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Re: PF cakes and isolates [Re: Doc_T]
#12970892 - 07/29/10 08:13 AM (1 year, 6 months ago) |
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Quote:
Doc_T said:
I'm doing clones on agar -> lc for cakes. Works great.
I would think this would be the easier method. Just make some LC with a piece of colonized agar, and then inject that into normally prepared BRF jars. I'm guessing that's how Doc_T does it?
The LC should colonize the jar just as fast, if not faster than an agar wedge I would think, since it would come in contact with more of the BRF at once.
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mukhail
Creeper
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Posts: 868
Loc: Antarctica
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Re: PF cakes and isolates [Re: mukhail]
#12970898 - 07/29/10 08:18 AM (1 year, 6 months ago) |
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Im definitely gonna try both and see what I prefer, but I have a feeling the outcome will lean towards LC.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 34,902
Loc: Colorado
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Re: PF cakes and isolates [Re: phaseflux]
#12971249 - 07/29/10 09:50 AM (1 year, 6 months ago) |
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Quote:
phaseflux said: Just make some LC with a piece of colonized agar, and then inject that into normally prepared BRF jars. I'm guessing that's how Doc_T does it?
And/or agar->grain for Grain LC and starting malt lc.
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hamloaf
Hamington Loafberg
Registered: 12/23/09
Posts: 6,063
Loc: The Electric Guitar.
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Re: PF cakes and isolates [Re: mukhail]
#12971366 - 07/29/10 10:23 AM (1 year, 6 months ago) |
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Quote:
mukhail said:
Excellent!
Ill have to start making silicon injection port jar lids then.
Don't forget filter material i.e. tyvek and micropore tape, synthetic filter disks, or Whatman filters. I use painters cover-all's tyvek and micropore tape over holes drilled in lids for GE. Drill 4 small 1/16th inch holes right next to each other in a diamond or square pattern. Place filter material on like this.
Tyvek.
Micropore tape
Tyvek.
Micropore tape.
Replace filter material every 2- 3 times through the PC. I replace mine every other time through the PC.
HL
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