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InvisiblePsiloKitten
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Re: The Importance of Gas Exchange [Re: Anno]
    #1232501 - 01/19/03 09:24 PM (9 years, 4 months ago)

Okay, I have a question on the inverse... during casing do you notice that alot of air is actually a detriment? I've found that the most tightly closed casings during regrow, turned out the best--


....but filter lids are key for jars, they work fantastic with the masking tape method described above.


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OfflineBladeLSD
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Re: The Importance of Gas Exchange [Re: Bi0TeK]
    #1234947 - 01/20/03 05:17 PM (9 years, 4 months ago)

Couldnt I just skip the lids completly possibly add a slightly thicker dry verm and just let them sit in the incubator lidless, Theoretically wouldnt this provide the most amount of gas exchange.


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Invisibledeanofmean
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Re: The Importance of Gas Exchange [Re: BladeLSD]
    #1234982 - 01/20/03 05:38 PM (9 years, 4 months ago)

that would be overkill, i think .and you might have to humidify your incubator .


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: PsiloKitten]
    #1235009 - 01/20/03 05:50 PM (9 years, 4 months ago)

> I've found that the most tightly closed casings during regrow, turned out the best

In the post casing - pre pinning phase the CO2 levels should be high. This is best achieved by simply covering the casing container with aluminum foil or similar.
It will get some air exchange(same as the jars with the aluminum foil lid), but the CO2 level will be high. That?s what you want to achieve.


Edited by Anno (01/29/03 03:11 AM)


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InvisiblePsiloKitten
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Re: The Importance of Gas Exchange *DELETED* [Re: Anno]
    #1235022 - 01/20/03 05:56 PM (9 years, 4 months ago)

Post deleted by PsiloKitten


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: PsiloKitten]
    #1235035 - 01/20/03 06:05 PM (9 years, 4 months ago)

You mean for the time after casing?

Well, if you seal the casing container, you will seal some fresh air in from outside?(at the top of the casing)
Since you are only incubating the casing in the closed container for a couple of days, and the substrate is already colonized to a high degree, the oxygen enclosed in the container seems to suffice for the mycelium to permeate the casing.

It?s the same with sealed PF jars, they grow for some time, but at a certain moment they stall.


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: Anno]
    #1257485 - 01/29/03 03:09 AM (9 years, 3 months ago)

TEST II :
This time I took the same plastic containers, but fitted them with a polyfill filter.
This is day 6 of colonization, look what a difference the air exchange makes!



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Offlinedankcrop
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Re: The Importance of Gas Exchange [Re: Anno]
    #1257534 - 01/29/03 03:38 AM (9 years, 3 months ago)

If the polyfill gets wet while steaming (I have no pc) would that be a problem?


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InvisibleClosetCase
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Re: The Importance of Gas Exchange [Re: Anno]
    #1257580 - 01/29/03 04:05 AM (9 years, 3 months ago)

Just to summerize (see if my thinking is correct):

The amount of gas exchange that the jars provide doesn't matter until there is a decent amount of growth (>20%)... sealed jars are ok for the beginning of colonization.

Then the mycelium start to consume the oxygen and change the ratio of co2/o2 inside the jar. This is when gas exchange becomes important, and jars that allow gas exchange colonize faster.

I am just trying to make everything perfectly clear 'cause i'm on my first grow with PF tek, and some of my jars have breathable tape over the holes and some have masking tape (ran out of breathable). I am considering taking the masking tape off and covering those ones with foil, but i want to wait since it is dry here and i am worried about the substrate drying out.

Thanks


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Offlinebjkroll
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Re: The Importance of Gas Exchange [Re: Anno]
    #1257663 - 01/29/03 04:48 AM (9 years, 3 months ago)

Hmm I always take the foil off :\


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Offlinesluglee
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Re: The Importance of Gas Exchange [Re: Anno]
    #1257751 - 01/29/03 05:13 AM (9 years, 3 months ago)

i am very interested in those plastic containers.. what are they made of ? how did you sterilize them ?


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: dankcrop]
    #1258031 - 01/29/03 06:50 AM (9 years, 3 months ago)

>If the polyfill gets wet while steaming (I have no pc) would that be a problem?
No, it dries in a matter of hours.

>The amount of gas exchange that the jars provide doesn't matter until there is a >decent amount of growth (>20%)... sealed jars are ok for the beginning of
>colonization.Then the mycelium start to consume the oxygen and change the
>ratio of co2/o2 inside the jar. This is when gas exchange becomes important,
>and jars that allow gas exchange colonize faster.
Yes, sounds about right.

>i am very interested in those plastic containers.. what are they made of ?
Polypropylene.

> how did you sterilize them ?
In the pressure cooker. It?s always a good idea of making a test run in the pressure cooker lbefore making a big batch, in case they do melt.
As for those containers, I inoculated them by opening the lid and inoculating them with a syringe in front of a flow hood.
But you could melt some holes in the lid before filling and inoculate them the "classical" way.


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Offlineteaker
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Re: The Importance of Gas Exchange [Re: Anno]
    #1258062 - 01/29/03 07:02 AM (9 years, 3 months ago)

what are the brand of those containers? and where did yo get them? those look like they would work better in my pc


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: teaker]
    #1258497 - 01/29/03 09:28 AM (9 years, 3 months ago)



Edited by Anno (01/29/03 09:34 AM)


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: Anno]
    #1263835 - 01/30/03 10:22 PM (9 years, 3 months ago)

One has to love polyfill !
Day 8:


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Anonymous

Re: The Importance of Gas Exchange [Re: Anno]
    #1263862 - 01/30/03 10:43 PM (9 years, 3 months ago)

Thoes are nice little containers. I'm sure anyone can find something similar in their local area.

Anon, do you only use thoes now or do you still use jars?

I'm nobody.


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: ]
    #1263865 - 01/30/03 10:44 PM (9 years, 3 months ago)

Both.


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Offlinemikejwill
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Re: The Importance of Gas Exchange [Re: Anno]
    #1264053 - 01/31/03 12:31 AM (9 years, 3 months ago)

Anno,  you are so hot :smile:


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OfflineAnnoA
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Re: The Importance of Gas Exchange [Re: mikejwill]
    #1264068 - 01/31/03 12:38 AM (9 years, 3 months ago)

I am, true :wink: , but the grl in my avatar is Audrey Tautou. 


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OfflineBunBoy
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Re: The Importance of Gas Exchange [Re: Anno]
    #1264525 - 01/31/03 04:31 AM (9 years, 3 months ago)

Audrey Tautou.
sigh....    :wink: 


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Cakes

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