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Offlinedirtlambguy
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Another Strain Isolation Question
    #12540414 - 05/11/10 12:15 AM (4 years, 5 months ago)

And,

I know its possible to start a strain isolation project via spore print, mushroom tissue, or colonized grain.  I was planning on starting my project with mushroom tissue, but I have a spore print and a jar of colonized grain.  Is it best to start a project with a cloned mushroom, or does it matter? 

-Nig


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InvisibleDoc_T
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Re: Another Strain Isolation Question [Re: dirtlambguy]
    #12540426 - 05/11/10 12:16 AM (4 years, 5 months ago)

'best' to start from spores, but any of those will work well.


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InvisibleJitsu
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Re: Another Strain Isolation Question [Re: Doc_T]
    #12540484 - 05/11/10 12:25 AM (4 years, 5 months ago)

With spores, you will have more options to work with and you will obtain true isolates. The drawback is that you will be spending more time obtaining single sector isolates from spores as opposed to clones. I really enjoy agar work though so I tend to just use spores.


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Offlinenugjug
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Re: Another Strain Isolation Question [Re: dirtlambguy]
    #12540490 - 05/11/10 12:26 AM (4 years, 5 months ago)

Cloning from a fruit body is easier and faster because you know it will be a fruiting strain.  That being said Doc is right about spores being best as you have more genetic variation to pick from.  However picking is the problem.  Once you isolate several strains you have to fruit them individually to test for potency, growth rates, size of flushes, etc.

Edit: Jitsu beat me to it.


Edited by nugjug (05/11/10 12:27 AM)


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Re: Another Strain Isolation Question [Re: nugjug]
    #12540569 - 05/11/10 12:40 AM (4 years, 5 months ago)

I remember reading something about: If a sector has rizomorphic growth then it has the proper genetics to fruit. Is this correct? If so then you would know if your sector is capable of fruiting, because you wouldn't isolate a sector that isn't rizomorphic anyways.


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OfflineGiggle_Grower
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Re: Another Strain Isolation Question [Re: Seagoat]
    #12540585 - 05/11/10 12:43 AM (4 years, 5 months ago)

i dont think all rhizo can fruit, but its pretty rare to find a non fruiting 'strain'. just my .02


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InvisibleJitsu
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Re: Another Strain Isolation Question [Re: Giggle_Grower]
    #12540633 - 05/11/10 12:51 AM (4 years, 5 months ago)

Rhizomorph is said to be the most prolific pinning type of mycelium for cubensis, but some of my best pinning strains (isolates) are tomentose appearing in growth. I've isolated many strains over the last couple years and have yet to see one of them not fruit.

Pretty sure it just comes down to compatible hyphae, rather than the growth pattern.(as far as fruiting strains go)


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Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there.

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Edited by Jitsu (05/11/10 12:57 AM)


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Re: Another Strain Isolation Question [Re: Jitsu]
    #12540687 - 05/11/10 01:03 AM (4 years, 5 months ago)

Quote:

Jitsu said:
Rhizomorph is said to be the most prolific pinning type of mycelium for cubensis, but some of my best pinning strains (isolates) are tomentose appearing in growth. I've isolated many strains over the last couple years and have yet to see one of them not fruit.

Pretty sure it just comes down to compatible hyphae, rather than the growth pattern.(as far as fruiting strains go)




Cool, thanks for sharing. I'm about to get my hands dirty with some agar isolations.


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