Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Cultivation Supplies   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemadi
Hullu Mykologi
Male User Gallery


Folding@home Statistics
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
Using hand blender as eberbach * 2
    #12401907 - 04/16/10 02:24 AM (13 years, 11 months ago)

There was something mentioned about blender in these forums, but I did write my own anyway. Because eberbach tools are insanely overpriced, I decided to try how it can be done with a hand blender from my home. It has a separate blade part, which I sterilized in autoclave. The results were exellect, colonizing did start much faster than from agar slices. I used way too much cultures and water, much less would have done the same. There wasnt much extra work, like 10-15 minutes. Species was P. azurescens.

The tools
Ready grown agar cultures
Hand blender with sterilized blade part
Scalpell, needles and syringes
Jar with water sterilized
Some readily sterilized unused grain jars

Preparing the tools.


There was too much water in the jar.


I cut the agar plates and put them in the jar.


Mixed with blender.


Took lc to syringes.


Inoculated grains. In 2,5 days there was quite nicely growth all around the jars.


After 7 days smaller jars were ready.


And larger ones in very nice growth.


And no contaminatios, I worked with flowhood. I was very happy with results!

Edited by madi (01/28/11 12:15 PM)

Extras: Filter Print Post Top
Invisiblebadman
Male User Gallery

Registered: 06/14/06
Posts: 4,039
Re: Using hand blender as eberbach [Re: madi]
    #12402259 - 04/16/10 06:09 AM (13 years, 11 months ago)

That is sweeet.

Extras: Filter Print Post Top
Offlinetalkingwalnut
Stranger


Registered: 10/03/08
Posts: 546
Last seen: 4 months, 4 days
Re: Using hand blender as eberbach [Re: badman]
    #12402440 - 04/16/10 07:32 AM (13 years, 11 months ago)

The process seems cool but I can't see this not getting contams eventually.


--------------------

Extras: Filter Print Post Top
Invisiblebadman
Male User Gallery

Registered: 06/14/06
Posts: 4,039
Re: Using hand blender as eberbach [Re: talkingwalnut]
    #12402490 - 04/16/10 07:58 AM (13 years, 11 months ago)

Flow hood should make this nice and easy.

Extras: Filter Print Post Top
Offlinescatmanrav
Brainy Smurf


Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 2 months
Trusted Cultivator
Re: Using hand blender as eberbach [Re: badman]
    #12402665 - 04/16/10 09:04 AM (13 years, 11 months ago)

Pretty cool. I dont know if you saw this before..its from a while ago...

http://www.shroomery.org/forums/showflat.php/Number/6421852#6421852

If you dont have a hand blender..try a regular blender with a detatchable blade. I've found my blade also fits right onto a jar.

You can get more blades here for 7 bucks:

http://www.goodmans.net/get_item_os-010_blender-blade-cutter-for-oster-osterizer-blenders.htm

Maybe try this hand blender with cakes. Using above tek you can have quart jars done in 3 days.

http://forums.mycotopia.net/spawning-teks/30494-tv-guide-colonizing-quart-jar-three-days.html

Not to take away from OP's idea...either one are pretty good tools.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

Extras: Filter Print Post Top
Offlinemadi
Hullu Mykologi
Male User Gallery


Folding@home Statistics
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
Re: Using hand blender as eberbach [Re: talkingwalnut]
    #12412825 - 04/18/10 03:11 AM (13 years, 11 months ago)

Quote:

talkingwalnut said:
The process seems cool but I can't see this not getting contams eventually.




When working with flowhood big enough the only risk I see is the motor part of the hand blender and Im wiping it with alcohol before working every time.

Scatmanrav: Thanks for the links, I'll look into it and try some variations.

Extras: Filter Print Post Top
Offlinescatmanrav
Brainy Smurf


Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 2 months
Trusted Cultivator
Re: Using hand blender as eberbach [Re: madi]
    #12420019 - 04/19/10 11:36 AM (13 years, 11 months ago)

Man that flowhood in your signature is sweeeeeet. Working with that you'd have an easy time doing just about anything..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

Extras: Filter Print Post Top
Invisiblebadman
Male User Gallery

Registered: 06/14/06
Posts: 4,039
Re: Using hand blender as eberbach [Re: scatmanrav]
    #12421208 - 04/19/10 03:19 PM (13 years, 11 months ago)

you know!

Extras: Filter Print Post Top
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: Using hand blender as eberbach [Re: badman]
    #12421297 - 04/19/10 03:36 PM (13 years, 11 months ago)

Of course you can make that work with your flowhood!. BASTARD!!! :lol:


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?

Extras: Filter Print Post Top
Invisiblemyco.alchemist
been there ~ done that

Registered: 03/27/10
Posts: 705
Loc: Flag
Trusted Cultivator
Re: Using hand blender as eberbach [Re: madi]
    #12421591 - 04/19/10 04:44 PM (13 years, 11 months ago)

Madi,

Do you have a stir plate?

If so, it will work as a blender.

Simply replace the stir bar with a small metal rod.

Dice the agar plate content into small pieces first.
That makes it blend faster.

Of course, for bigger jobs, these are sweet.


--------------------
CASING = GETTING IT RIGHT

Extras: Filter Print Post Top
Invisiblemyco.alchemist
been there ~ done that

Registered: 03/27/10
Posts: 705
Loc: Flag
Trusted Cultivator
Re: Using hand blender as eberbach [Re: myco.alchemist]
    #12422990 - 04/19/10 08:17 PM (13 years, 11 months ago)



Tiny wedge off agar perti to an LC on a stir plate.

Extras: Filter Print Post Top
Offlinemadi
Hullu Mykologi
Male User Gallery


Folding@home Statistics
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
Re: Using hand blender as eberbach [Re: myco.alchemist]
    #12424119 - 04/19/10 11:28 PM (13 years, 11 months ago)

No I don't have a stir plate. They aren't so pricy so I might get one some day, but it's not on top of my list. There are sometimes cheap used ones and if I find one in a right day I'll get it. Sounds interesting, that plate should keep the mycelium broken if I use some of it later. I really need to get some bigger syringes, it's pain to use 20 ml with lots of LC. Yeah, that flowhood makes my life easier. I took a risk with buying it used but the filters were fully working. I should take a new picture of it now when it's in use.

Extras: Filter Print Post Top
Invisiblemyco.alchemist
been there ~ done that

Registered: 03/27/10
Posts: 705
Loc: Flag
Trusted Cultivator
Re: Using hand blender as eberbach [Re: madi]
    #12424187 - 04/19/10 11:44 PM (13 years, 11 months ago)



Stir plate will increase LC colonization time 2 or 3 X.
Plus, they get thick as gravy.

Also, shreds MYC into tiny particles.
So, you don't plug syringes & get more points of contact.

Back in the day, when I did bigger scale edibles.
I used a gun to innoc grain spawn bags.
I would connect to a gallon LC & go for it.

http://www.stonemfg.net/images/brochures/Socorex/Socorex%20(High%20Res).pdf


--------------------
CASING = GETTING IT RIGHT

Extras: Filter Print Post Top
Offlinemadi
Hullu Mykologi
Male User Gallery


Folding@home Statistics
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
Re: Using hand blender as eberbach [Re: myco.alchemist]
    #12559097 - 05/13/10 11:01 PM (13 years, 10 months ago)

I think this type of blender would make it easier, it has a separate bowl part:



I also noticed that when mixing smaller amounts you get easily annoying foam if you mix it too long periods. Just a few secs at the time. I got 60 ml syringes now too.

Extras: Filter Print Post Top
Invisibleteknix
π“‚€βŸπ“…’π“π“…ƒπ“Š°π“‰‘ 𓁼𓆗⨻
 User Gallery

Registered: 09/16/08
Posts: 11,953
Re: Using hand blender as eberbach [Re: madi]
    #12559125 - 05/13/10 11:06 PM (13 years, 10 months ago)

Thats a great idea,but wouldn't it be simpler just to innoc a jar of agar in the 1st place?

Extras: Filter Print Post Top
Offlinemadi
Hullu Mykologi
Male User Gallery


Folding@home Statistics
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
Re: Using hand blender as eberbach [Re: teknix]
    #12559212 - 05/13/10 11:23 PM (13 years, 10 months ago)

Quote:

teknix said:
Thats a great idea,but wouldn't it be simpler just to innoc a jar of agar in the 1st place?




For Fungiperfectis homepage:

Eberbach Containers
Fungi Perfecti stocks Eberbach containers for devotees of the liquid inoculation technique. These containers are autoclavable. Partially fill with water, autoclave in your pressure cooker. Transfer an entire dish of mushroom mycelium into it. Place the container on a stirrer, activate, and in five seconds or less there are millions of tiny fragments of hyphae which can be transferred into sterilized grain. This technique minimizes contamination, accelerates colonization, maximizes the potential of your mycelia, and tremendously speeds up the inoculation/incubation process. The cultures and time you save will more than make the cost worthwhile. One culture grown on a 100 x 15mm petri dish can effectively inoculate 100–300 cups of grain using these stirrers! In fact, some mushroom species grow too slowly via the traditional methods of propagation, and defeat would-be spawn makers. With these stirrers, mycelium of difficult-to-grow species can be expanded quickly and efficiently. In our opinion, these stirrers are integral to any professional spawn laboratory.

Extras: Filter Print Post Top
Invisibleteknix
π“‚€βŸπ“…’π“π“…ƒπ“Š°π“‰‘ 𓁼𓆗⨻
 User Gallery

Registered: 09/16/08
Posts: 11,953
Re: Using hand blender as eberbach [Re: madi]
    #12559293 - 05/13/10 11:39 PM (13 years, 10 months ago)

Yeah, I meant just skip the whole transfer it to jar from petri dish step. It would still be the samething in the end, You would just skip pouring the petri dishes and innoc right to the jar its already in. Or is the petri dish step vital in some way?

Extras: Filter Print Post Top
Invisiblemyco.alchemist
been there ~ done that

Registered: 03/27/10
Posts: 705
Loc: Flag
Trusted Cultivator
Re: Using hand blender as eberbach [Re: madi]
    #12559312 - 05/13/10 11:43 PM (13 years, 10 months ago)



GRIN,

Waited, watched & finally STOLE one of these on ebay years back.
Its an old WARING lab blender, but solid as steel.
$40 buck, including shipping

Tough thing about some other types, is they will not autoclave.
This puppy, I autoclave the containers in a bag, with water IN the container.
That way, you know water & container are both STERILE


--------------------
CASING = GETTING IT RIGHT

Extras: Filter Print Post Top
Offlinemadi
Hullu Mykologi
Male User Gallery


Folding@home Statistics
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
Re: Using hand blender as eberbach [Re: teknix]
    #12559462 - 05/14/10 12:16 AM (13 years, 10 months ago)

Quote:

teknix said:
Yeah, I meant just skip the whole transfer it to jar from petri dish step. It would still be the samething in the end, You would just skip pouring the petri dishes and innoc right to the jar its already in. Or is the petri dish step vital in some way?




Oh now I see what you meant. I find petri dishes easier to use and manage and you can do lots of them and choose good ones instead of making lots of agar jars. But I guess you could do it straing in to jar. But then you can't sterilize the water there, so you need to pour something anyway.

Extras: Filter Print Post Top
Offlinemadi
Hullu Mykologi
Male User Gallery


Folding@home Statistics
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
Re: Using hand blender as eberbach [Re: talkingwalnut]
    #13865675 - 01/28/11 12:20 PM (13 years, 2 months ago)

Quote:

talkingwalnut said:
The process seems cool but I can't see this not getting contams eventually.




Now after over 20 times done and not a single contamination I can say it is all about your sterile technique. I have changed the TEK a bit: I only use one agar culture. I also either pour LC directly to jars/bags or use syringes without needles.

This is now the way I use most when inoculating grains. It is very fast and reliable TEK.

I edited pics, now most of them will show.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Cultivation Supplies   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* Eberbach containers at fungi perfecti Visigoth 4,205 15 03/01/22 11:38 PM
by AphexPin
* Joining The Eberbach Family! hamloaf 2,778 15 01/17/15 04:55 PM
by eatyualive
* Liquid Culture- Magnetic Stir plate or Eberbach? mushpunx 1,154 3 02/06/16 03:38 AM
by mushpunx
* Eberbach for Long Term Storage of Mycelium in Distilled Water - Anastomosis? eltrkbrd 3,424 15 09/10/07 11:22 AM
by eVenom
* New Blender!
( 1 2 3 4 all )
total 7,248 79 01/14/11 11:50 PM
by GroosBrewz
* Eberbach alternatives??? Elemicin 1,093 5 09/15/15 01:10 AM
by djbluntmagic
* Eberbach base questions chobumms 607 2 09/08/09 12:11 AM
by chobumms
* Autoclavable Blender Base Cascad3 4,180 5 04/11/12 08:07 PM
by Fishermanj

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
5,023 topic views. 23 members, 102 guests and 106 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.007 seconds on 12 queries.