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madi
Hullu Mykologi
Registered: 01/27/09
Posts: 414
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Last seen: 10 years, 7 months
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Using hand blender as eberbach 2
#12401907 - 04/16/10 02:24 AM (13 years, 11 months ago) |
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There was something mentioned about blender in these forums, but I did write my own anyway. Because eberbach tools are insanely overpriced, I decided to try how it can be done with a hand blender from my home. It has a separate blade part, which I sterilized in autoclave. The results were exellect, colonizing did start much faster than from agar slices. I used way too much cultures and water, much less would have done the same. There wasnt much extra work, like 10-15 minutes. Species was P. azurescens.
The tools Ready grown agar cultures Hand blender with sterilized blade part Scalpell, needles and syringes Jar with water sterilized Some readily sterilized unused grain jars
Preparing the tools.
There was too much water in the jar.
I cut the agar plates and put them in the jar.
Mixed with blender.
Took lc to syringes.
Inoculated grains. In 2,5 days there was quite nicely growth all around the jars.
After 7 days smaller jars were ready.
And larger ones in very nice growth.
And no contaminatios, I worked with flowhood. I was very happy with results!
Edited by madi (01/28/11 12:15 PM)
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Using hand blender as eberbach [Re: madi]
#12402259 - 04/16/10 06:09 AM (13 years, 11 months ago) |
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That is sweeet.
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talkingwalnut
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Re: Using hand blender as eberbach [Re: badman]
#12402440 - 04/16/10 07:32 AM (13 years, 11 months ago) |
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The process seems cool but I can't see this not getting contams eventually.
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Using hand blender as eberbach [Re: talkingwalnut]
#12402490 - 04/16/10 07:58 AM (13 years, 11 months ago) |
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Flow hood should make this nice and easy.
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scatmanrav
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Re: Using hand blender as eberbach [Re: badman]
#12402665 - 04/16/10 09:04 AM (13 years, 11 months ago) |
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Pretty cool. I dont know if you saw this before..its from a while ago...
http://www.shroomery.org/forums/showflat.php/Number/6421852#6421852
If you dont have a hand blender..try a regular blender with a detatchable blade. I've found my blade also fits right onto a jar.
You can get more blades here for 7 bucks:
http://www.goodmans.net/get_item_os-010_blender-blade-cutter-for-oster-osterizer-blenders.htm
Maybe try this hand blender with cakes. Using above tek you can have quart jars done in 3 days.
http://forums.mycotopia.net/spawning-teks/30494-tv-guide-colonizing-quart-jar-three-days.html
Not to take away from OP's idea...either one are pretty good tools.
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madi
Hullu Mykologi
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
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Re: Using hand blender as eberbach [Re: talkingwalnut]
#12412825 - 04/18/10 03:11 AM (13 years, 11 months ago) |
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Quote:
talkingwalnut said: The process seems cool but I can't see this not getting contams eventually.
When working with flowhood big enough the only risk I see is the motor part of the hand blender and Im wiping it with alcohol before working every time.
Scatmanrav: Thanks for the links, I'll look into it and try some variations.
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scatmanrav
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Re: Using hand blender as eberbach [Re: madi]
#12420019 - 04/19/10 11:36 AM (13 years, 11 months ago) |
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Man that flowhood in your signature is sweeeeeet. Working with that you'd have an easy time doing just about anything..
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badman
Registered: 06/14/06
Posts: 4,039
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Re: Using hand blender as eberbach [Re: scatmanrav]
#12421208 - 04/19/10 03:19 PM (13 years, 11 months ago) |
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you know!
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myceleus_rex
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Registered: 07/01/09
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Re: Using hand blender as eberbach [Re: badman]
#12421297 - 04/19/10 03:36 PM (13 years, 11 months ago) |
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Of course you can make that work with your flowhood!. BASTARD!!!
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myco.alchemist
been there ~ done that
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Re: Using hand blender as eberbach [Re: madi]
#12421591 - 04/19/10 04:44 PM (13 years, 11 months ago) |
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Madi,
Do you have a stir plate?
If so, it will work as a blender.
Simply replace the stir bar with a small metal rod.
Dice the agar plate content into small pieces first. That makes it blend faster.
Of course, for bigger jobs, these are sweet.
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myco.alchemist
been there ~ done that
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Tiny wedge off agar perti to an LC on a stir plate.
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madi
Hullu Mykologi
Registered: 01/27/09
Posts: 414
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Last seen: 10 years, 7 months
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No I don't have a stir plate. They aren't so pricy so I might get one some day, but it's not on top of my list. There are sometimes cheap used ones and if I find one in a right day I'll get it. Sounds interesting, that plate should keep the mycelium broken if I use some of it later. I really need to get some bigger syringes, it's pain to use 20 ml with lots of LC. Yeah, that flowhood makes my life easier. I took a risk with buying it used but the filters were fully working. I should take a new picture of it now when it's in use.
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myco.alchemist
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Re: Using hand blender as eberbach [Re: madi]
#12424187 - 04/19/10 11:44 PM (13 years, 11 months ago) |
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Stir plate will increase LC colonization time 2 or 3 X. Plus, they get thick as gravy.
Also, shreds MYC into tiny particles. So, you don't plug syringes & get more points of contact.
Back in the day, when I did bigger scale edibles. I used a gun to innoc grain spawn bags. I would connect to a gallon LC & go for it.
http://www.stonemfg.net/images/brochures/Socorex/Socorex%20(High%20Res).pdf
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madi
Hullu Mykologi
Registered: 01/27/09
Posts: 414
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Last seen: 10 years, 7 months
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I think this type of blender would make it easier, it has a separate bowl part:
I also noticed that when mixing smaller amounts you get easily annoying foam if you mix it too long periods. Just a few secs at the time. I got 60 ml syringes now too.
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teknix
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Registered: 09/16/08
Posts: 11,953
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Re: Using hand blender as eberbach [Re: madi]
#12559125 - 05/13/10 11:06 PM (13 years, 10 months ago) |
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Thats a great idea,but wouldn't it be simpler just to innoc a jar of agar in the 1st place?
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madi
Hullu Mykologi
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
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Re: Using hand blender as eberbach [Re: teknix]
#12559212 - 05/13/10 11:23 PM (13 years, 10 months ago) |
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Quote:
teknix said: Thats a great idea,but wouldn't it be simpler just to innoc a jar of agar in the 1st place?
For Fungiperfectis homepage:
Eberbach Containers Fungi Perfecti stocks Eberbach containers for devotees of the liquid inoculation technique. These containers are autoclavable. Partially fill with water, autoclave in your pressure cooker. Transfer an entire dish of mushroom mycelium into it. Place the container on a stirrer, activate, and in five seconds or less there are millions of tiny fragments of hyphae which can be transferred into sterilized grain. This technique minimizes contamination, accelerates colonization, maximizes the potential of your mycelia, and tremendously speeds up the inoculation/incubation process. The cultures and time you save will more than make the cost worthwhile. One culture grown on a 100 x 15mm petri dish can effectively inoculate 100β300 cups of grain using these stirrers! In fact, some mushroom species grow too slowly via the traditional methods of propagation, and defeat would-be spawn makers. With these stirrers, mycelium of difficult-to-grow species can be expanded quickly and efficiently. In our opinion, these stirrers are integral to any professional spawn laboratory.
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teknix
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Re: Using hand blender as eberbach [Re: madi]
#12559293 - 05/13/10 11:39 PM (13 years, 10 months ago) |
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Yeah, I meant just skip the whole transfer it to jar from petri dish step. It would still be the samething in the end, You would just skip pouring the petri dishes and innoc right to the jar its already in. Or is the petri dish step vital in some way?
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myco.alchemist
been there ~ done that
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Posts: 705
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Re: Using hand blender as eberbach [Re: madi]
#12559312 - 05/13/10 11:43 PM (13 years, 10 months ago) |
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GRIN,
Waited, watched & finally STOLE one of these on ebay years back. Its an old WARING lab blender, but solid as steel. $40 buck, including shipping
Tough thing about some other types, is they will not autoclave. This puppy, I autoclave the containers in a bag, with water IN the container. That way, you know water & container are both STERILE
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madi
Hullu Mykologi
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
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Re: Using hand blender as eberbach [Re: teknix]
#12559462 - 05/14/10 12:16 AM (13 years, 10 months ago) |
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Quote:
teknix said: Yeah, I meant just skip the whole transfer it to jar from petri dish step. It would still be the samething in the end, You would just skip pouring the petri dishes and innoc right to the jar its already in. Or is the petri dish step vital in some way?
Oh now I see what you meant. I find petri dishes easier to use and manage and you can do lots of them and choose good ones instead of making lots of agar jars. But I guess you could do it straing in to jar. But then you can't sterilize the water there, so you need to pour something anyway.
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madi
Hullu Mykologi
Registered: 01/27/09
Posts: 414
Loc: North-Europe
Last seen: 10 years, 7 months
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Quote:
talkingwalnut said: The process seems cool but I can't see this not getting contams eventually.
Now after over 20 times done and not a single contamination I can say it is all about your sterile technique. I have changed the TEK a bit: I only use one agar culture. I also either pour LC directly to jars/bags or use syringes without needles.
This is now the way I use most when inoculating grains. It is very fast and reliable TEK.
I edited pics, now most of them will show.
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