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 deozone
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 Culture of Cordyceps sinensis and Cordyceps militaris 
#11986207 - 02/08/10 03:57 AM (2 years, 3 months ago) |
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I am a medical doctor in Thailand doing research on using mushroom for treating patients. I want to get Cordyceps sinensis and Cordyceps militaris culture. Anyone want to donate or exchange those strains with medicinal mushroom strains in my collection please pm me.
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c-ray
lovin' it
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 Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: deozone]
#11986216 - 02/08/10 04:12 AM (2 years, 3 months ago) |
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deozone
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: c-ray]
#11989866 - 02/08/10 04:51 PM (2 years, 3 months ago) |
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Yes, It is me. The strain I use is Cordyceps militaris.
Some persons contacted me offering trading with the strain of Cordyceps militaris which is enough for my research.
So, I wish to stop trading for Cordyceps militaris.
Thank you all of you for that.
The strain that I am still looking for is Cordyeps sinensis. If anyone want to trade with me please pm. Thank you again.
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 Speeker
www.speeker.tk
Registered: 02/11/04
Posts: 609
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: deozone]
#11993226 - 02/09/10 01:52 AM (2 years, 3 months ago) |
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About a year ago the Ministry of the Health of Thailand ordered C.militaris
(MushroomPeople strain) from FreeSporePrints (Fabio).
By any change is that the very same culture?
What method you used to fruit it?
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deozone
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 Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: Speeker]
#11993254 - 02/09/10 02:11 AM (2 years, 3 months ago) |
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No, It is not the same culture. I tried many methods on the strain I got from Fabio but it did not work. I think it may be the problem of that strain itself that cannot fruit. So, I changed the strains. I just got it fruit a few weeks ago.
I shared the method I used with wbastz. The method I used is not perfect. So, I am experimenting the new method to fruit it.
Are you Fabio?
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Speeker
www.speeker.tk
Registered: 02/11/04
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: deozone]
#11993292 - 02/09/10 02:29 AM (2 years, 3 months ago) |
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No, I'm the one who sent that stubborn strain to Freesporeprints (= Fabio)
to be spreaded around.
Quote:
Male doctor delightfully Thai eggplant burn cultivation "mushroom body rental" successfully helped his brother to work.
The manager ASTV online. January 29, 2553 15:38 pm
Male doctor delightfully Thai eggplant burn cultivation "mushroom body rental" and Thailand's first successful blow.A similar drug properties reveal a ultra-sensitive. Solve problems with sexual performance degenerative substances "nitric oxide" has resulted in the blood to the sex organs increased.
On January 29, MD. Somis fame established head of AIDS. Bureau of AIDS, tuberculosis and infectious diseases, sexually Department of Disease Control Ministry of Health (St.) today announced the successful cultivation of mushroom tank rental. Help in the treatment of physical symptoms that sexual degeneration.To test the fiber of his body rent a mushroom culture itself since 2550 as properties of mushrooms found a drug that helps treat many diseases including cancer, kidney disease, it also helps the blood to the sex organs. further. Can help people with degenerative problems have physical sex. With properties similar to a drug Amazing ultra-sensitive. Because of the mushroom body is a substance called rent nitric oxide, a substance that is located in the artery wall cells personnel. When eating mushrooms cylinder rental will receive this message. And will result in the blood to the sex organs increased.
"Normal mushroom body rental prices are very high as thousands baht per kg. It will find these mushrooms have a high specific area, such as in China and Tibet, this type of mushroom is very difficult. His trial has started a thread of this type of mushroom cultivation in Thailand itself. The trial began on 3 years ago and the success can be cultured for up to a mushroom. Which they bring culture into the fiber of mushrooms in a small glass bottle. Within a rice vitamins that through steam sterilization ago. When put into the fiber of mushrooms, then put into the refrigerator. And control the temperature 10 degrees C for 2 months found that mushrooms are the flower. The data from the study also found that in Thailand who can not rent a tank mushroom cultivation has not. Most will be able to culture the only fiber only "MD. Somis said.
MD. Somis said that the way to eat mushrooms tank rental. Can be done several ways to dry skin and bringing fresh or used to mix the drink, which has begun to note that the implementation of a mix of coffee and then some. And from this trial will be adjusted on the flower food to make mushroom faster.
Somis said that rent for mushroom body. Mushroom Kingdom are living in a few minutes until the worm larvae died in the winter. When the summer mushroom species will germinate from the worms will be found only in high mountain of Bhutan, China, Tibet is very diverse properties such as help in respiratory cough air bags aneurysm bronchitis cause of action. Kidney better. Make the body fresh memory improved blood sugar levels. Reduce blood pressure reduce inflammation anticancer increase landscape resistance of the body. Against free radicals so slow senility.
http://translate.google.com/translate?u=http%3A//www.manager.co.th/QOL/ViewNews.aspx%3FNewsID%3D9530000013146&hl=en&langpair=auto|en
hmm.. Thai doesn't translate well with Google..
Edited by Speeker (02/09/10 02:38 AM)
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weskes
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: Speeker]
#11993428 - 02/09/10 03:54 AM (2 years, 3 months ago) |
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No it doesn't. I would like to know more about growing this cordyceps.
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 fungus_tao
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: weskes]
#11993488 - 02/09/10 04:32 AM (2 years, 3 months ago) |
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I was listening to this interview with Stamets yesterday and he talks about how it's easier to get at the medicines (polysacharides, beta-glucans, etc) in mycellium form because the the mycellium is only 1 cell wall thich, as opposed to fruiting mushrooms which are much harder to digest. Interesting food for thought.
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SOUTHERN
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: fungus_tao]
#11994461 - 02/09/10 09:33 AM (2 years, 3 months ago) |
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deozone nice work.. could u shed some light one the fruiting substrate or do u wish to keep that secret.. would love to know and best of luck with your studies.
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treal
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: fungus_tao]
#11995389 - 02/09/10 12:09 PM (2 years, 3 months ago) |
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Quote:
fungus_tao said:
I was listening to this interview with Stamets yesterday and he talks about how it's easier to get at the medicines (polysacharides, beta-glucans, etc) in mycellium form because the the mycellium is only 1 cell wall thich, as opposed to fruiting mushrooms which are much harder to digest. Interesting food for thought.
Interesting. If you did an alcohol extraction, it wouldn't matter much either way, right?
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fungus_tao
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: treal]
#11995989 - 02/09/10 01:36 PM (2 years, 3 months ago) |
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Listen to the interview. They discuss in detail why mycellium is better for full spectrum medcinal production than fruits, or fruit extractions. Not trying to put down deozones hard efforts to successfully fruit cordyceps because that is 
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SOUTHERN
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: fungus_tao]
#11996868 - 02/09/10 03:46 PM (2 years, 3 months ago) |
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so deozone talk to us about your research and the needs of the cordyceps. this community knows alot and would love to help if we can, just as much as we would love to learn from you.
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deozone
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: SOUTHERN]
#11996951 - 02/09/10 03:58 PM (2 years, 3 months ago) |
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This is the formula that I use in growing the mycelium at 12 c for about 2 months in dark and raise the temperature to 18-22 c and turn on the fluorescent light 24 hrs to stimulate fruiting body. I use LUX meter to measure the light intensity which is around 700 LUX.
cornmeal 10 gram
glucose, 10 gram
meat peptone 10 gram
yeast extract 5 gram
potassium dihydrogen phosphate 1 gram
magnesium sulfate 0.5 gram
vitamin B1 50 mg
add all of them to 500 cc water solution
I put 30 gram of unpolished jasmine rice in bottle and 60 cc of solution then autoclave,then I put the mycelium to grow on it.
I use the temperature control which can adjust the range of temperature between 1-2 c around the set point.
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deozone
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: deozone]
#11996990 - 02/09/10 04:02 PM (2 years, 3 months ago) |
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I want to get Cordyceps sinensis strain to run my research project to use it as a supplement to test if it can help people who have kidney failure or not ? If anyone can help, that will be great.
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curenado
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: SOUTHERN]
#11997028 - 02/09/10 04:07 PM (2 years, 3 months ago) |
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I would do mycelium based meds here as the temperatures won't fruit
cordyceps naturally for enough of a season as I understand it.
It is the one mushroom we technically have to buy in and at this point that's what we do using whole fruit powder.
There's a place called fungi health which sells a fruit&mycelium extract that I've been wanting to compare but cordyceps is definitely a choice medical mushroom.
I use a combo of reishi & cordyceps (1:1) as a first try for erectile dysfunction and if that doesn't do it I get more severe but it does the trick for a lot of guys.
But for all general purposes, cordyceps is a staple at our clinic with most people eating it.
As far as I know we have been using sinensis - how does one get a militaris culture to grow out? Would love to work with that...
<<is very diverse properties such as help in respiratory cough air bags aneurysm bronchitis cause of action. Kidney better. Make the body fresh memory improved blood sugar levels. Reduce blood pressure reduce inflammation anticancer increase landscape resistance of the body. Against free radicals so slow senility.>>
Lol! If you translate that out it's all true and well known. Stamets pulled a lot of his references from Asian sources. Dr. Somis seems to be focusing on arresting/reversing necrosis of the kidney tissue and I can't say I've seen that detailed anywhere. The type and degree of benefit could change the donor waiting list radically to say the least.
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Yours in the Natural State!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."
Edited by curenado (02/09/10 08:24 PM)
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SOUTHERN
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: curenado]
#11997794 - 02/09/10 06:00 PM (2 years, 3 months ago) |
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THANKS FOR THE INSIGHT DEOZONE. so u basically cook up a special nutrient inriched liquid culture then grow it out on jasmine rice. have u tried other substrates (brown rice ,wheat , common spawn substrates)? Does this food (refering to substrate) still produce the same medical value that naturally occuring cordyceps produces? Have u identified any compounds in the fruits/myceluim.
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curenado
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: curenado]
#11997903 - 02/09/10 06:14 PM (2 years, 3 months ago) |
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Dr. Somis ~
I not sure about kidney failure, but I use cordyceps as part of treatment for kidney stones. We use reishi, cordyceps and turkey tail (1:1:1) and a 4 herb formula to soften and expel the stones. I believe you are on the right track with cordyceps and kidneys. The kidney stone tx we are using is the most effective and painless I have seen.
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Yours in the Natural State!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."
Edited by curenado (02/09/10 06:17 PM)
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deozone
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: curenado]
#11999939 - 02/10/10 12:55 AM (2 years, 3 months ago) |
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This method is just the one that I can make it fruit. I have not try other methods with the strain I have. Of course, I had tried different method with the strain I got it from Fabio but it failed. I have not sent it to analyze its medicinal components yet. My priority now is to standardize the growing condition and media for Cordyceps militaris culture and look for Cordyceps sinensis strain.
Good to hear some results of treatment from curenado !!!
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BlimeyGrimey
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: deozone]
#12000674 - 02/10/10 07:10 AM (2 years, 3 months ago) |
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Have you thought about using other Cordyceps species, such as C. ophioglossoides?
Have you studied the anamorphs for possible medical properties?
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I've done no harm, I keep to myself. There's nothing wrong with my state of mental health.
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deozone
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: BlimeyGrimey]
#12003748 - 02/10/10 03:46 PM (2 years, 3 months ago) |
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No, I focus only on these two species. I have not studied much about anamorph.
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wbastz
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: deozone]
#12007312 - 02/11/10 05:11 AM (2 years, 3 months ago) |
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Hi Dr.,
I was thinking about testing your methodology with the strain of Cordyceps militaris received from Roger Fudd and see what happens . I´ve started a new experiment this week and I could include this formula together the other tests.
Cheers,
Wilson
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Jonat
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: fungus_tao]
#12007355 - 02/11/10 05:39 AM (2 years, 3 months ago) |
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Quote:
fungus_tao said:
Listen to the interview. They discuss in detail why mycellium is better for full spectrum medcinal production than fruits, or fruit extractions. Not trying to put down deozones hard efforts to successfully fruit cordyceps because that is
It was a good Stamets interview, but I think it is a glittering generality to say that mycelium is always better than the fruitbody. Many plants and fungi have important chemicals ('secondary metabolites') that are normally only produced at certain stages of the life cycle. For example, reishi spores may have useful biochemicals that are not in the mycelium.
Industrial microbiologists will try to vary growing conditions to trick the mycelia to produce these secondary metabolites, or they will develop mutated strains that have those genes turned on all the time. If one is trying to make a million pounds of antibiotics or statins, that approach makes sense. But sometimes a whole mushroom or whole herb is best.
One of the arguments is that mycelium is finer and easier to extract - but we can mechanically grind most ingredients pretty easily. Experience shows that extracts with water, alcohol, and other simple solvents does a good job for most natural medicines.
And thanks for sharing, Deozone!!
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curenado
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: Jonat]
#12008464 - 02/11/10 10:21 AM (2 years, 3 months ago) |
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<<They discuss in detail why mycellium is better for full spectrum medcinal production than fruits, or fruit extractions. >>
Actually, that's only true in mushrooms where the mycelial and fruit body properties you want are identical like Jonat said.
In some the mycelial production by-products are beneficial as well in immune stimulation but not all or we could just grow mycelium for everything here.
Not to start a great big thing but - "Scientifically" speaking Stamets hasn't been reliable for "the truth, the whole truth and nothing but the truth" in some time - he will publish that you want the hot water extractbles but then sell alcohol extracts at a premium without mentioning the difference.
Paul traded the science wagon for the profit/status whore cart a while ago and even people that were formerly devout groupies have become disillusioned. It's even gotten so blatant that you can say "Oh Bull" on shroomery and not immediately be attacked by mindless raving groupies - didn't used to be that way, but things change.
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Yours in the Natural State!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."
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deozone
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: BlimeyGrimey]
#12058385 - 02/19/10 03:25 AM (2 years, 3 months ago) |
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If anyone has Hirsutella sinensis, anamorph of Cordyceps sinensis, please pm me. It maybe useful if I cannot find Cordyceps sinensis.
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wbastz
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: deozone]
#12058492 - 02/19/10 05:05 AM (2 years, 3 months ago) |
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Hi,
Could someone explain what is an anamorph of Cordyceps sinensis and also tell me where I could find a good image of Cordyceps sinensis mycelium, with explanations of its physical characteristics ?
If I can isolate the mycelium from the caterpillar, which will be the name of the mycelium ? Cordyceps sinensis or Hirsutella sinensis mycelium ? How to differentiate them visually ?
Cheers,
Wilson
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wbastz
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: wbastz]
#12140239 - 03/04/10 04:45 AM (2 years, 2 months ago) |
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Hello everybody,
Has anyone had any answers about what I asked?
Cheers
Wilson
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BlimeyGrimey
Collector of Spores
Registered: 08/24/05
Posts: 3,738
Loc: Puget Sound
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Re: Culture of Cordyceps sinensis and Cordyceps militaris [Re: wbastz]
#12142546 - 03/04/10 01:40 PM (2 years, 2 months ago) |
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Cordyceps sinensis is the sexual form of the organism, also known as a telemorph. It produces the Cordyceps fruiting bodies.
Hirsutella sinensis is the asexual form of the organism, also known as the anamorph. The anamorph is usually found living in soil, and is more mold like in appearance, as in it produces spores on stalks like bread mold.
If you isolated the fungus from an insect it would be the Cordyceps species, if it started to sporulate like mold does, then it has reverted to the anamorphic form. I'm not quite sure what conditions are necessary for it to change forms.
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I've done no harm, I keep to myself. There's nothing wrong with my state of mental health.
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