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Protocal said:
Couple of questions, I've read in a few other places that after you inoculate keeping the jars heated to around 80 degrees F using a heating pad or heated water is suggested to help the jars colonize faster. Is that necessary or can I just leave em sitting in my closet at room temperature? Also I've heard that using a humidifier or fish tank bubbler is better than misting because introducing so much water to the colonized cake's exterior can greatly slow down the pinning and production cycle. And since there will be holes drilled through out the FC the FAE wouldn't be as much of a problem as it normally is due to the high humidity levels. Is there any truth to that? And is it really necessary to have such an advanced system of humidification if there's Perlite at the bottom of the FC or will misting suffice? I just don't wanna go and drop the extra cash for a fish tank bubbler PLUS the stones and everything if all I'm going to need is a fine mist spray bottle and a paper fan after I get my colonized cakes into my FC. If you're telling me all I will need after dunking and rolling my cakes in verm is my FC, enough Perlite to fill 3"-4" of my FC, some tin foil squares to place the cakes on, and a mist bottle, you just saved me a good $50-$75 I was planning on spending on heaters/humidifiers and such.

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Shea25 said:
PF tek
Things needed

1/2 pint wide mouth canning jars
Pot/Pressure cooker
Vermiculite
Perlite
Brown rice/Brow Rice Flour
Tin foil
70% Iso alcohol
Plastic Tote
6500K florescent bulb
1/4 inch drill bit/Drill
Hammer/Nail
Mixing bowl and spoon
Gypsum
Spore syringe
Latex gloves
Dust mask
Lighter



Preparing the jar

Take the lids and using a nail and hammer or a 1/8 drill bit, drill 4 holes near the rim of the lids nicely spaced out. Depending what way you use you lids i use mine rubber side down just punch the holes so they don’t leave burrs pointing towards you.

The basic recipe for 5 PF jars is this 2 parts vermiculite 1 part water and 1 part BRF (additionally you can add 1 tablespoon of gypsum for 5 jars)

In a bowl add the 2 cups of vermiculite (and gypsum if you use it) now add the 1 cup of water, mix really well. Now add the 1 cup of Brown rice flour (you can make your own by grinding brow rice in a coffee grinder) Mix this really really well. Now load this into the 5 canning jar equally, making sure not to compress the mixture in the jars you want it nice and airy. You will be leaving a 1/2 inch space at the tops of the jars for the dry vermiculite layer.

Take a paper towel and wipe the 1/2inch space you left at the tops of the jars making sure to wipe away any BRF mixture or moisture away. Now top off the jars with dry vermiculite, screw on the lids and cover the tops the lids with tin foil. The tin foil is there to keep any moisture from entering the jars while there sterilizing.













Sterilizing

Now you want to take you PC or (pot with a tight fitting lid) and fill the bottom of it with jar lid rings and tin foil this is to keep the bottom of your jars off the bottom of the PC/Pot so they don’t burn Some PC’s come with trays to keep the jar already off the bottom use that if you can. Once that is done put the jars into the PC/pot and fill it with cold water just almost to the bottom of the jars. Now put on the lid of the PC/Pot and put the heat on High. Once the rocker on top of the PC starts to rock turn the heat right down to just keep it to a slight rock and start your 60 minute count down, If you’re using a pot once it come to a boil turn it down to a slight simmer and start your 90 minute count down. After the 60(PC) or 90(Pot) minutes are up turn off the heat and walk away let them cool down over night or for 8 hours. 






Inoculation

Once the jars are cooled down put them in a draft free room along with the 70% Iso,Lighter,spore syringe . Make sure the rooms windows are shut and AC or heater turned off. Wash your hands and arms really good. Now put of the gloves and mask Take the foil lids off the jars you won’t need them anymore. Now rub your hand down with the 70% ISO wait a few seconds for it to evaporate. Now shake the syringe really well, using the lighter heat the needle of the syringe till its red hot now proceed to inoculate the jars using a 1/4 cc of solution per hole inoculating towards the glass. That’s 1 cc per jar.  After every jar heat the needle back till its red hot and repeat.





Incubation

Now put the jars away in a closet of shelf at room temp they will start to colonize in a few days. Keep an eye on them every 4 days or so. Once they appear to be 100% colonized let them sit for another week to consolidate.



Birthing and dunk

Once the jars are finished their 1 week consolidation it’s ready to get them out of the jars. Open the jars get rid of the dry vermiculite layer now slap the jars (upside down) onto a wood cutting board or counter top, trying to coax the cakes out of the jars. Once out rinse them off and submerge in cool water for 24 hours making sure they stay submerged.  After 24 hours rinse them again and now roll the cake in dry vermiculite making sure to cover the cake well tops and bottoms.
I find busting up some of the verm for the dry roll up alittle finer helps.













Fruiting

Build one of these http://www.mushroomvideos.com/Terrarium-Tek

I personally use a PMP

Mist your cakes 3-4 times a day and fan right after, fan 5-6 times a day. Water evaporating off your cakes is a major pinning trigger. Give them a 12/12 light schedule using a 6500K Florescent bulb (daylight or cool blue). Once they have pinned and grown and all picked, dunk them for 24 hours again this time no dry roll in vermiculite and put back in the Shotgun Fruiting Chamber.

Good Luck

Tips

Gypsum really helps the development of the fruit bodies I recommend using it
Room temp 70-75 is perfect for incubation and fruiting.
There’s no need to keep the dunking cakes in the fridge
Mist and Fan Mist and Fan
Use short wide mouth 1/2 pint jars
Dont cover the lids after inoculation with anything example, Foil/tape
Give them love

Results in this  Golden Teacher


This was 2 cakes first flush, came just under 19 grams dry. Oak Ridge


Heres another set of cakes





Results may vary



Here is a How to on sterilizing a syringe to make up a spore syringe

How to sterilize a syringe with just a pot water and syringe 

Thing needed.

A pot
Empty Syringe
Water(tap can work)


Fill the pot with cold water. turn the burner on High and wait for the water to come to a full boil. now start a 10 minute countdown and keep the water at a full boil.

After 10 minutes take the empty syringe and suck back the boiling water and let it sit  in the syringe for 1 minute then expel the water in a sink(not back in the pot)

Repeat 4 more times on the last time keep the water in the syringe cap it and let it cool down.

Hope this helps.







x4







Notes:
Use a Q-tip and 70% alcohol to clean the inside of the cap
I have found even after months and months they will stay sterile
No need to store in the fridge
Never try to cool them down fast in the fridge

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Sylus said:
Thanks to Shea25!

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flexorman said:
Can i use 350ml wide-mouth jars?
I dont find smaller ones,how long will these colonize and how much solution i must inject to 1 jar?

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k00laid said:

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Sylus said:
Thanks to Shea25!

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+1

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ghettosteve17 said:
why is this teks perlite soaking wet???

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