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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Gypsum, Herb Grinder, Perlite, Pressure Cooker, Vermiculite

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InvisibleShea25
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Pf tek done right TEK * 41
    #11585613 - 12/04/09 08:28 PM (4 years, 9 months ago)

PF tek
Things needed

1/2 pint wide mouth canning jars
Pot/Pressure cooker
Vermiculite
Perlite
Brown rice/Brow Rice Flour
Tin foil
70% Iso alcohol
Plastic Tote
6500K florescent bulb
1/4 inch drill bit/Drill
Hammer/Nail
Mixing bowl and spoon
Gypsum
Spore syringe
Latex gloves
Dust mask
Lighter



Preparing the jar

Take the lids and using a nail and hammer or a 1/8 drill bit, drill 4 holes near the rim of the lids nicely spaced out. Depending what way you use you lids i use mine rubber side down just punch the holes so they don’t leave burrs pointing towards you.

The basic recipe for 5 PF jars is this 2 parts vermiculite 1 part water and 1 part BRF (additionally you can add 1 tablespoon of gypsum for 5 jars)

In a bowl add the 2 cups of vermiculite (and gypsum if you use it) now add the 1 cup of water, mix really well. Now add the 1 cup of Brown rice flour (you can make your own by grinding brow rice in a coffee grinder) Mix this really really well. Now load this into the 5 canning jar equally, making sure not to compress the mixture in the jars you want it nice and airy. You will be leaving a 1/2 inch space at the tops of the jars for the dry vermiculite layer.

Take a paper towel and wipe the 1/2inch space you left at the tops of the jars making sure to wipe away any BRF mixture or moisture away. Now top off the jars with dry vermiculite, screw on the lids and cover the tops the lids with tin foil. The tin foil is there to keep any moisture from entering the jars while there sterilizing.













Sterilizing

Now you want to take you PC or (pot with a tight fitting lid) and fill the bottom of it with jar lid rings and tin foil this is to keep the bottom of your jars off the bottom of the PC/Pot so they don’t burn Some PC’s come with trays to keep the jar already off the bottom use that if you can. Once that is done put the jars into the PC/pot and fill it with cold water just almost to the bottom of the jars. Now put on the lid of the PC/Pot and put the heat on High. Once the rocker on top of the PC starts to rock turn the heat right down to just keep it to a slight rock and start your 60 minute count down, If you’re using a pot once it come to a boil turn it down to a slight simmer and start your 90 minute count down. After the 60(PC) or 90(Pot) minutes are up turn off the heat and walk away let them cool down over night or for 8 hours. 






Inoculation

Once the jars are cooled down put them in a draft free room along with the 70% Iso,Lighter,spore syringe . Make sure the rooms windows are shut and AC or heater turned off. Wash your hands and arms really good. Now put of the gloves and mask Take the foil lids off the jars you won’t need them anymore. Now rub your hand down with the 70% ISO wait a few seconds for it to evaporate. Now shake the syringe really well, using the lighter heat the needle of the syringe till its red hot now proceed to inoculate the jars using a 1/4 cc of solution per hole inoculating towards the glass. That’s 1 cc per jar.  After every jar heat the needle back till its red hot and repeat.





Incubation

Now put the jars away in a closet of shelf at room temp they will start to colonize in a few days. Keep an eye on them every 4 days or so. Once they appear to be 100% colonized let them sit for another week to consolidate.



Birthing and dunk

Once the jars are finished their 1 week consolidation it’s ready to get them out of the jars. Open the jars get rid of the dry vermiculite layer now slap the jars (upside down) onto a wood cutting board or counter top, trying to coax the cakes out of the jars. Once out rinse them off and submerge in cool water for 24 hours making sure they stay submerged.  After 24 hours rinse them again and now roll the cake in dry vermiculite making sure to cover the cake well tops and bottoms.
I find busting up some of the verm for the dry roll up alittle finer helps.













Fruiting

Build one of these http://www.mushroomvideos.com/Terrarium-Tek

I personally use a PMP

Mist your cakes 3-4 times a day and fan right after, fan 5-6 times a day. Water evaporating off your cakes is a major pinning trigger. Give them a 12/12 light schedule using a 6500K Florescent bulb (daylight or cool blue). Once they have pinned and grown and all picked, dunk them for 24 hours again this time no dry roll in vermiculite and put back in the Shotgun Fruiting Chamber.

Good Luck

Tips

Gypsum really helps the development of the fruit bodies I recommend using it
Room temp 70-75 is perfect for incubation and fruiting.
There’s no need to keep the dunking cakes in the fridge
Mist and Fan Mist and Fan
Use short wide mouth 1/2 pint jars
Dont cover the lids after inoculation with anything example, Foil/tape
Give them love

Results in this  Golden Teacher


This was 2 cakes first flush, came just under 19 grams dry. Oak Ridge


Heres another set of cakes





Results may vary



Here is a How to on sterilizing a syringe to make up a spore syringe


How to sterilize a syringe with just a pot water and syringe 

Thing needed.

A pot
Empty Syringe
Water(tap can work)


Fill the pot with cold water. turn the burner on High and wait for the water to come to a full boil. now start a 10 minute countdown and keep the water at a full boil.

After 10 minutes take the empty syringe and suck back the boiling water and let it sit  in the syringe for 1 minute then expel the water in a sink(not back in the pot)

Repeat 4 more times on the last time keep the water in the syringe cap it and let it cool down.

Hope this helps.







x4







Notes:
Use a Q-tip and 70% alcohol to clean the inside of the cap
I have found even after months and months they will stay sterile
No need to store in the fridge
Never try to cool them down fast in the fridge


Edited by Shea25 (06/17/10 12:52 AM)


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Invisiblebadman
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Registered: 06/14/06
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Re: Pf tek done right [Re: Shea25] * 2
    #11585645 - 12/04/09 08:34 PM (4 years, 9 months ago)

I remember one of your cake grows it was badass.


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InvisibleShea25
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Re: Pf tek done right [Re: badman] * 1
    #11585674 - 12/04/09 08:37 PM (4 years, 9 months ago)

Using this method I was constantly getting 6-9 gram first flushes. Around  13 grams dry per cake. the one in my avatar came out to 7.6 dry first flush


Edited by Shea25 (12/04/09 08:37 PM)


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OfflineLeopardMan
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Re: Pf tek done right [Re: Shea25] * 1
    #11585726 - 12/04/09 08:43 PM (4 years, 9 months ago)

Awesome thread :congrats:


--------------------
Either you care, or you don't (Stanley Kubrick)


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OfflineShroomerRoss
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Re: Pf tek done right [Re: LeopardMan] * 1
    #11585745 - 12/04/09 08:46 PM (4 years, 9 months ago)

Quote:

13 grams dry per cake


:shocked:


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Invisiblebadman
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Re: Pf tek done right [Re: ShroomerRoss] * 1
    #11585780 - 12/04/09 08:51 PM (4 years, 9 months ago)

Quote:

ShroomerRoss said:
Quote:

13 grams dry per cake


:shocked:




Trust, hes a sickhead!


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OfflineJabbawaya

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Re: Pf tek done right [Re: badman] * 1
    #11585879 - 12/04/09 09:06 PM (4 years, 9 months ago)

Nice summary! Simple and easy to follow for a new grower.


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OfflineCyanesense
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Re: Pf tek done right [Re: badman] * 1
    #11585896 - 12/04/09 09:08 PM (4 years, 9 months ago)

I know they say not to cover it after inoculation but that just doesnt seem to make sense in my mind.... after being so sterile why leave open holes?
does the dry verm protect it that well??


--------------------
:eatingout:

Everything I say is totally false - and all my pictures were stolen from SWIM.:bananabang:


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InvisibleShea25
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Re: Pf tek done right [Re: Cyanesense] * 1
    #11585904 - 12/04/09 09:09 PM (4 years, 9 months ago)

It sure does I have done TONNES of PF's and the dry verm barrier has not let me down. You will see. I have told so many people when there asking why are my jars going so slow and it because people cover the holes. Theres just no need to, or whats the point of the dry verm? might as well build self healing lids with a tyvek GE port


Edited by Shea25 (12/04/09 09:24 PM)


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OfflineCyanesense
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Re: Pf tek done right [Re: Shea25] * 1
    #11586111 - 12/04/09 09:39 PM (4 years, 9 months ago)

hmmm... interesting!
I have micropore tape over my holes, your saying I might as well go take it off without worrying about F*ing my shit up?


--------------------
:eatingout:

Everything I say is totally false - and all my pictures were stolen from SWIM.:bananabang:


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InvisibleShea25
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Re: Pf tek done right [Re: Cyanesense] * 1
    #11586125 - 12/04/09 09:40 PM (4 years, 9 months ago)

lol I would say start saving that micropore tape for other applications. Its a waste to use it over the innc holes. Its not the cheapest stuff


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OfflineCyanesense
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Re: Pf tek done right [Re: Shea25] * 1
    #11586142 - 12/04/09 09:43 PM (4 years, 9 months ago)

good to know! :thumbup:
Thanks for the easy to understand user friendly tek :super:


--------------------
:eatingout:

Everything I say is totally false - and all my pictures were stolen from SWIM.:bananabang:


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OfflineDragonkill14
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Re: Pf tek done right [Re: Shea25] * 1
    #11586151 - 12/04/09 09:44 PM (4 years, 9 months ago)

Do you find that a top lay or pure verm is not needed? How is your contam to not ratio?


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InvisibleShea25
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Re: Pf tek done right [Re: Dragonkill14] * 1
    #11586192 - 12/04/09 09:49 PM (4 years, 9 months ago)

The top dry verm layer is a must. I have a 99.9% successful PF rate. Only time I had a contam was when I tried an experimental technique


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OfflineRogerRabbitM
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Re: Pf tek done right [Re: Dragonkill14] * 1
    #11586210 - 12/04/09 09:51 PM (4 years, 9 months ago)

The top layer of dry verm is required.  That's the filter that stops contaminant spores from reaching the moist rice flour below.  The tape is optional. We show the tape over holes in the DVD, to demonstrate going the extra mile.  If you're in an area with a very high contaminant spore load, use it.  If not, skip it.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat


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InvisibleShea25
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Re: Pf tek done right TEK [Re: Shea25] * 1
    #11586249 - 12/04/09 09:57 PM (4 years, 9 months ago)

Here are some Self healing Filtered GE port PF tek lids I made



These also with a dry verm barrier your basically 100% not going to get any contams from the outside at least


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OfflineCyanesense
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Re: Pf tek done right [Re: RogerRabbit] * 1
    #11586274 - 12/04/09 10:00 PM (4 years, 9 months ago)


These are jars I made up 2 weeks ago, I took this picture early yesterday morning.
The Taz (on the left) showed and started growth before the Cambos(on the right.) but the cambos have EXPLODED past the taz. a very fast, agressive colonizer once established.
cant wait to get some LC started!!!c'mon payday:biggrin:


--------------------
:eatingout:

Everything I say is totally false - and all my pictures were stolen from SWIM.:bananabang:


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OfflineCyanesense
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Re: Pf tek done right [Re: Cyanesense] * 1
    #11586288 - 12/04/09 10:02 PM (4 years, 9 months ago)

whats the point of the tyvek?
GE is not needed for colonization, just wondering why you would have one?
I could understand if you used the lids for both LC/grain and PF cakes.


--------------------
:eatingout:

Everything I say is totally false - and all my pictures were stolen from SWIM.:bananabang:


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InvisibleShea25
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Re: Pf tek done right [Re: Cyanesense] * 1
    #11586291 - 12/04/09 10:03 PM (4 years, 9 months ago)

Looking sweet :thumbup:. A little advice thoe i would not be putting my jars on the ground especially carpet. Most mold spores and contams are only 1 foot from the ground. Im sure you fine but its good to know


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InvisibleShea25
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Re: Pf tek done right [Re: Cyanesense] * 1
    #11586297 - 12/04/09 10:04 PM (4 years, 9 months ago)

Gas Exchange is most certainly needed for colonization. FAE is not


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Gypsum, Herb Grinder, Perlite, Pressure Cooker, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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