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HorseBox said:
I'm bringing this post back from the dead because this warrants further experimentation. I just skimmed through the thread but didn't see the OP post the final results. I started a thread on this I'll post all my experiments there.

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jimbotron said:
That or he's working off-Broadway...

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Example 1

4.5 g LABS (linear alkylbenzene sulfonates) were dissolved in 95 ml of distilled water and then added to a solution containing 0.32 g of silver nitrate. After careful mixing, 0.2 grams of hydrazine solution (0.03 M) was added to the solution, so a yellow colloidal solution of silver was formed. The UV-Vis spectrum of the reaction solution showed an absorption band at around 415 nm which is the characteristic absorption band of silver nanoparticles. The results of dynamic light of the reaction solution, shown in Figure 2 (A) show that the average particle size is 4.5 nm.


Example 2

The same process as in Example 1 was repeated, except that the 4.5 g LABS have been replaced by 4.5 g SDS (sodium dodecylsulfate). The UV spectrum of the colloidal solution of silver nanoparticles obtained is shown in Figure 1, and the average size of particles was 78.2 nm as shown in Figure 2 (B).


Example 3

Tween 20 (polysorbate 20) was used instead of LABS in Example 1. The UV spectrum of the colloidal solution of silver nanoparticles obtained is shown in Figure 1 and the average size of particles was 32.5 nm as shown in Figure 2 (C). Example 4

The colloidal silver nanoparticles prepared in Examples 1-3 were examined to determine the antimicrobial activity of the colloid.

1. Provide a culture medium of sterile nutrient agar (NA) and spread pure.

2. Provide a microbial suspension of standard strain of bacteria E. coli (ATCC 25 922) to the process of McFarland, that is to say, a concentration of 15 <8> cfu / ml.

3. Provide a microbial suspension of a concentration of 10 5 from the suspension above.

4. Combine 1 / 3 of colloid nanoparticles of silver and 2 / 3 of bacteria provided.

5. Growing mixed product obtained in the previous step the culture medium of nutrient agar. 6. Place the plates in the incubator at 25 [deg.] C for 24 h.

7. After 24 hours, take a handle on these plates and put on a new medium of nutrient agar.

8. After 24 h in the incubator, the plates look as regards the number of bacterial colonies. Results:

No colony or any signs of bacterial growth was observed on the plate in the experimental group.

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