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 anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
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Mine colonized in the light and I saw no noticeable difference.
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The above post is fictional, hypothetical, or downright nonsensical.
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 deathblade
Stranger
Registered: 06/25/06
Posts: 155
Last seen: 1 month, 16 days
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 Re: A cloning Question [Re: anonjon]
#11259353 - 10/16/09 10:28 AM (2 years, 7 months ago) |
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It has been 5 days. All I can see is rhizomorhic growth. There is only a little bit of fuzz growing on the pieces. It looks like most of the growth is in the agar, not on the surface. I would put up pics but my camera doesnt work well enough. I think that I can see some sectoring. How long would you guys leave it before starting the next transfer?
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anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
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If one sector is big nuff you could just take out a piece and inoc a grain jar now. Then go to grain lc when that jar colonizes.
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The above post is fictional, hypothetical, or downright nonsensical.
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deathblade
Stranger
Registered: 06/25/06
Posts: 155
Last seen: 1 month, 16 days
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Re: A cloning Question [Re: anonjon]
#11259586 - 10/16/09 11:09 AM (2 years, 7 months ago) |
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I am trying to isolate it. I have more jars started so I am not worried about losing the strain.
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anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
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I dunno why you'd want to wait before you transfer it again. Go for it.
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The above post is fictional, hypothetical, or downright nonsensical.
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SirBeatNikCat
Ron Paul 2012 or DIE.
Registered: 11/11/02
Posts: 110
Loc: Newport,OR USA 
Last seen: 1 month, 2 days
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Re: A cloning Question [Re: anonjon]
#11370469 - 11/02/09 03:00 PM (2 years, 6 months ago) |
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I wanted to make a comment on when they are to be cloned:
If you take them as they are smaller, when they "vigourous," it wont make the cloned shroom vigorous, just because.
if you take it when it is fully fruited, and you can see the results, then you know the way it fruits and such.
Either way you will get the same result, from the same fruit.
Im not a expert or shroomin like those here, But I do know genetics.
DNA is DNA.
If you take a clone of a rose in the spring because it grows vigorously, and then expect the clone to do the same, it wont be true, unless you give it the right enviroment to do so.
I think I would wait then take the clone when the results were known.
peace,
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"All wrong-doing arises because of mind. If the mind is transformed can wrong-doing remain?"
"The Buddha"
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,425
Loc: Berlin, Germany
Last seen: 19 minutes, 50 seconds
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Yes, DNA is DNA. The difference I see, lies in epigenetics, in other words in the activation pattern of the genes. So when taking a tissue sample, which is currently in fast growing mode, mycelium will grow faster, compared to a sample, which already stopped growing or - in the worst case - entered dying mode after the fruit body reached full maturity. So it has to change gene activation, before it can start growing, while in the first case it just continues.
Carsten
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anonjon
Partially Right
Registered: 11/03/08
Posts: 6,322
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Re: A cloning Question [Re: Mycelio]
#11375970 - 11/03/09 09:37 AM (2 years, 6 months ago) |
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Is that why i couldn't clone myself by putting my boogers on agar?
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The above post is fictional, hypothetical, or downright nonsensical.
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,425
Loc: Berlin, Germany
Last seen: 19 minutes, 50 seconds
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Re: A cloning Question [Re: anonjon]
#11376329 - 11/03/09 10:39 AM (2 years, 6 months ago) |
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Yes, better use a large biopsy needle and extract a tissue sample from the inside of your balls, alright?
Carsten
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cloudsaregathering
pasturbater
Registered: 08/08/09
Posts: 1,264
Loc: ZZ9 Plural Z Alpha
Last seen: 6 hours, 55 minutes
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Re: A cloning Question [Re: Mycelio]
#11386696 - 11/04/09 06:01 PM (2 years, 6 months ago) |
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whoaaa... boys...
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"the root of the problem has been isolated"
Trade List
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SirBeatNikCat
Ron Paul 2012 or DIE.
Registered: 11/11/02
Posts: 110
Loc: Newport,OR USA
Last seen: 1 month, 2 days
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Mycelio,
If I am to understand If I took a clone from a little shroom that was growing quickly,( and if I could,) took another clone of it when it was mature, then I would see two different growth patterns,
One quick, from its youthful vigor, and the other slower,because of age.
But both contain the exact DNA.
I know from cannabis that from seed you get way better growth then from clones.
because the clones are of an older age, and dont have that vigorous stage they had at their beginngs from seed.
I am certainly no expert on this, as with shrooms I am somewhat guessing at best.
But it seems that when mycelium begins to produce shrooms thats its "vigorous stage."
However from a fully fruited shroom, it would be passed that stage, however when the clones where fruited they would hit the vigorous stage same as with any other all others at that point.
But I dont think you can capture that stage for good by cloning young.
cause then you would have to hope they also fruit big as well, fast.
Plants and shrroms are two different things, so I could be totally off on this.
But I would like to know.
If its possable to capture the activation pattern, to pruduce faster, Thats great to know.
I would like to know more about it.
Mr K@
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"All wrong-doing arises because of mind. If the mind is transformed can wrong-doing remain?"
"The Buddha"
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,425
Loc: Berlin, Germany
Last seen: 19 minutes, 50 seconds
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Hi, I wasn't talking about age, that is a different story. What I meant is the current state, the mycelium is in. At pin stage, there is fast growth going on, while at mature stage this growth has stopped already and a few days later the mushroom tissue would start to die. I am sure there will be a big difference in growth during the first days after cloning, but I don't know for how long there will be a difference. I also don't think that you can capture that speedy or slow growth for the rest of the cultivation, but when starting with vigorous tissue, the next grow may be completed one or two weeks earlier.
If you are doing a multispore grow, you better wait for the mushrooms to reach full size, just to determine, which ones you like most. As RR, FF and Morelman discussed this earlier in this thread, each has its pros and cons.
For plants, everything seems to be different. While mushrooms and animals seem to be counting cell divisions by cutting of telomeres from the ends of the chromosomes, plants seem to be able to reset this mechanism if multiplied by cuttings or grafting.
Carsten
Edited by Mycelio (11/08/09 09:49 AM)
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