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 dstark
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 Re: Grain spawn without pressure cooking [Re: Mycelio]
#11291186 - 10/21/09 09:52 AM (2 years, 7 months ago) |
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Thnaks mate =] I'll keep posting
Edit:
Ok so i washed the grains in a strainer and put them in new container with water to just cover them. Then i completely closed the lid.
Question: Is there any way to increase the lactic acid bacteria population or give it any kind of boost?
Edited by dstark (10/21/09 10:04 AM)
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dstark
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Re: Grain spawn without pressure cooking [Re: dstark]
#11304419 - 10/23/09 03:25 AM (2 years, 7 months ago) |
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Ok bubbles, the water is turned only little bit not transparent, and some kind of smell lol!
so whats up with my questions mycelium?
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Mycelio
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Re: Grain spawn without pressure cooking [Re: dstark]
#11305989 - 10/23/09 11:51 AM (2 years, 7 months ago) |
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Quote:
dstark said:
...
Question: Is there any way to increase the lactic acid bacteria population or give it any kind of boost?
When using materials, that won't ferment easily, one can add sugar as a food source or citric acid to lower the PH. For grain this won't make sense.
Once you have a healthy fermentation going on, you can inoculate the next one with a few drops of fermentation water or a few kernels.
Carsten
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Doc_T
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Re: Grain spawn without pressure cooking [Re: dstark]
#11323823 - 10/26/09 10:47 AM (2 years, 6 months ago) |
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Quote:
dstark said:
Question: Is there any way to increase the lactic acid bacteria population or give it any kind of boost?
Lactobacillus is common available and easy to buy. I bet you could culture it and add it easily.
--------------------
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dstark
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Re: Grain spawn without pressure cooking [Re: Doc_T]
#11335582 - 10/27/09 10:07 PM (2 years, 6 months ago) |
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Yeah i know, they are present in yogurts, thought about adding some to the grains but i guess it would raise the chances for contam...
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Mycelio
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Re: Grain spawn without pressure cooking [Re: dstark]
#11336546 - 10/28/09 04:21 AM (2 years, 6 months ago) |
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There are dozens of Lactobacillus species. The ones that ferment milk are useless with grain. If you really want to buy microbes that are present everywhere, look for sourdough starter.
Carsten
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dstark
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Re: Grain spawn without pressure cooking [Re: Mycelio]
#11337919 - 10/28/09 10:56 AM (2 years, 6 months ago) |
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Well the grains are doing much batter now Carsten! there are bubbles and almost no yeast film + the water is pretty clear.
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Mycelio
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Re: Grain spawn without pressure cooking [Re: dstark]
#11344518 - 10/29/09 09:53 AM (2 years, 6 months ago) |
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That sounds good. I guess it is ready within a few days.
Carsten
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dstark
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Re: Grain spawn without pressure cooking [Re: Mycelio]
#11350625 - 10/30/09 07:56 AM (2 years, 6 months ago) |
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Ill post a pic very soon 
Some grains start to float so i remove them.
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blackout
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Re: Grain spawn without pressure cooking [Re: dstark]
#11356326 - 10/31/09 07:24 AM (2 years, 6 months ago) |
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I was thinking to stop floating grains you could put them in a bag with a few small holes in it, so water would seep into the bag but stop them rising.
Also this might allow for cleaner handling afterwards, just lift the bag up and it will drain itself. The filter on my autoclave bags is far too restrictive to allow air in easily but I do have other larger filter disks which allow free travel of air. I could have a large bag of grain with a large filter on top, lift the bag out and the water pours out, and clean filtered air is drawn into it. It might be better to have just one hole. You could even fill the bag first with water and have an injection silicone port at the bottom, then stick a needle in and wait for it to drain naturally.
I have drained off some bleaching grains I had in a PET bottle, they still look too wet, not like they are overcooked/soaked, just the surfaces are wet, I was thinking of heating them lightly, like to 50C just to try and get them to absorb the moisture. I have the cap on and opened it slightly and the smell seems OK.
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dstark
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Re: Grain spawn without pressure cooking [Re: blackout]
#11367855 - 11/02/09 08:20 AM (2 years, 6 months ago) |
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Lol i took a photo and forgot to post :/
 sorry for quality again ;( 
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dstark
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Re: Grain spawn without pressure cooking [Re: dstark]
#11373185 - 11/02/09 09:01 PM (2 years, 6 months ago) |
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The water is so much darker now, not the yeasty darkness but the kind of the grain was sitting to much in water...
I think it is ready now?!
Question:
Can i strain it, and than freeze it? i will have a petri culture going on only the next week or so, so i wounder how i can store the grains =]
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Mycelio
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Re: Grain spawn without pressure cooking [Re: dstark]
#11375718 - 11/03/09 08:59 AM (2 years, 6 months ago) |
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Yes, it should be ready by now.
When freezing, there will be ice crystals breaking the cell walls. Don't know if this will be good or bad.
As you are not working with a Pleurotus species, I recommend you dry the kernels in a microwave or oven, like Blackout described. Once dried and cooled down, you can store it for a long time. If you still plan to inoculate with LC, dry kernels will be better anyway.
Carsten
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blackout
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Re: Grain spawn without pressure cooking [Re: Mycelio]
#11377302 - 11/03/09 01:07 PM (2 years, 6 months ago) |
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Be very careful with the microwave, it can go on fire or burn. Keep stirring to stop hot/cold spots, an oven is probably better. Here is a thread on how hot it gets in a microwave.
http://www.shroomery.org/forums/showflat.php/Number/4327704#4327704
In this thread I grew on burnt grains, http://www.shroomery.org/forums/showflat.php/Number/4649222#4649222
Which worked surprisingly well.
The freezing gives me another idea, as a way to expand the grain, leaving it more like a sponge to soak up LC, or liquidised shrooms. Roasting also increases the volume like a sponge. If they were dried out they might still contain the antibiotics. At the end of a grow you could liquidise up a shroom or 2 and dump it on your now dry grain which it might suck up and colonise in no time.
I just defrosted some grains the other day and they did tend to explode a lot more during cooking, they were wet before, I think only partially soaking might have been better.
Edited by blackout (11/03/09 01:17 PM)
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dstark
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Re: Grain spawn without pressure cooking [Re: blackout]
#11382354 - 11/04/09 08:04 AM (2 years, 6 months ago) |
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I have a LC going on but i don't know what would be more preferable, LC or myc. tissue, i guess myc. tissue but i don't have any agar work going on for now so... I'll experiment with my LC- also will see if it is still viable for use
So as i understood:
Put it in some kind of glass jar, put in oven- [What temps?] until they are dry and inoculate with LC (of course after they have been cooled down xD). Should i strain it before i put in oven or put it with the whole load of water it has? O.o
1 more question poped in my head:
We do all this process to drop the pH, after drying out the water and putting LC the pH will go up again (LC is pretty basic)... What you think?!
Edited by dstark (11/04/09 08:07 AM)
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Mycelio
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Re: Grain spawn without pressure cooking [Re: dstark]
#11384716 - 11/04/09 01:54 PM (2 years, 6 months ago) |
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I'd strain and put the kernels as a thin layer on a plate or something flat. Regarding the temperature, Blackout used a pretty high setting. Anything above 65°C (pasteurization temp) will kill most of the microbes, but perhaps you better use something between 100 and 200°C to stay closer to Blackouts successful treatment.
And yes, the PH may rise, but that possibly makes it easier for your cube mycelium.
Carsten
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dstark
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Re: Grain spawn without pressure cooking [Re: Mycelio]
#11388006 - 11/04/09 09:00 PM (2 years, 6 months ago) |
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Ok so i decided not to risk the project and start now agar plates work... will be using myc. tissue.
You say i can preserve it by drying, but when i have a agar plates ready how do I dehydrate the grains?
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Mycelio
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Re: Grain spawn without pressure cooking [Re: dstark]
#11389785 - 11/05/09 08:41 AM (2 years, 6 months ago) |
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Quote:
dstark said:
Ok so i decided not to risk the project and start now agar plates work... will be using myc. tissue.
Alright.
Quote:
dstark said:
You say i can preserve it by drying, but when i have a agar plates ready how do I dehydrate the grains?
What is 'it'? Do you really mean dehydrate or rehydrate?
Carsten
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dstark
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Re: Grain spawn without pressure cooking [Re: Mycelio]
#11389927 - 11/05/09 09:11 AM (2 years, 6 months ago) |
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Quote:
Mycelio said:
Quote:
dstark said:
Ok so i decided not to risk the project and start now agar plates work... will be using myc. tissue.
Alright.
Quote:
dstark said:
You say i can preserve it by drying, but when i have a agar plates ready how do I dehydrate the grains?
What is 'it'? Do you really mean dehydrate or rehydrate?
Carsten
Sorry mispelled, I ment rehydrate, like making it with water again 
So how do I ? =]
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Mycelio
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Re: Grain spawn without pressure cooking [Re: dstark]
#11390486 - 11/05/09 10:52 AM (2 years, 6 months ago) |
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 Just add water and wait a few hours.
If your LC proves to be clean, I'd soak the grain in there.
Carsten
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