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Offlinetroncotron
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Registered: 12/13/08
Posts: 75
Loc: Spain
Last seen: 4 days, 11 hours
Re: Crossing P. Mexicana (sclerotia) and psilocybe cubensis! [Re: Cloneufc]
    #11389089 - 11/05/09 03:23 AM (2 years, 6 months ago)

You don´t need USB camera, look at this photos, i took them with a normal digital camera. In ebay you have 400X microscopes for 70 euros, it will work for your purpose.




Auxin, i prefer working with monokarions, for various reasons:

- Once meiosis and spore production has take place , you are going to have a huge diversity in the F1 descendant as a result of recombination, so you can choose and select your desired traits. If you are looking for a specific trait, working with dykarions is like a lotery. example: You will never isolate a red mushroom if the region of the crhomosome involved has not recombined,even in 10th generations... you will be loosing your time. If you cross  monokarions you have much more gene pool, the "red trait" already IS in someplace, and you could isolate it by doing many generations

If you work with dykarions, you can´t know how much cells have recombined and how much recombination has occured. You may have products with a 0,000001% of recombination that will never show traits of one parental.

Another problem i see is that you may isolate a product with cells that have recombined and others that not.

Please, correct me if i´m wrong, that´s only my opinion


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InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,738
Loc: Puget Sound
Re: Crossing P. Mexicana (sclerotia) and psilocybe cubensis! [Re: LadyLittleZeppelin]
    #11390633 - 11/05/09 11:31 AM (2 years, 6 months ago)

Quote:

LadyLittleZeppelin said:
It was hard enough to cross Penis Envy with PF Albino. What he is trying to do seems almost completely scientifically impossible :tongue:

Thus, it is a waste of his time.




How was injecting a jar of monokaryotic PFA mycelium with PE spores hard enough? Quite simple actually. The hardest part was getting a monokaryotic culture.


Quote:

troncotron said:
Auxin, i prefer working with monokarions, for various reasons:

- Once meiosis and spore production has take place , you are going to have a huge diversity in the F1 descendant as a result of recombination, so you can choose and select your desired traits. If you are looking for a specific trait, working with dykarions is like a lotery. example: You will never isolate a red mushroom if the region of the crhomosome involved has not recombined,even in 10th generations... you will be loosing your time. If you cross  monokarions you have much more gene pool, the "red trait" already IS in someplace, and you could isolate it by doing many generations

If you work with dykarions, you can�t know how much cells have recombined and how much recombination has occured. You may have products with a 0,000001% of recombination that will never show traits of one parental.

Another problem i see is that you may isolate a product with cells that have recombined and others that not.

Please, correct me if i�m wrong, that�s only my opinion




I haven't started my recombinant DNA classes yet, so I can't make a truly educated response, but I mostly agree with what you are saying.


--------------------
I've done no harm, I keep to myself. There's nothing wrong with my state of mental health.


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OfflineCloneufc
Master Exploder!
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Registered: 11/15/07
Posts: 1,223
Loc: Las Vegas Flag
Last seen: 30 days, 19 hours
Re: Crossing P. Mexicana (sclerotia) and psilocybe cubensis! [Re: troncotron]
    #11392202 - 11/05/09 03:40 PM (2 years, 6 months ago)

Quote:

troncotron said:
You don?t need USB camera, look at this photos, i took them with a normal digital camera. In ebay you have 400X microscopes for 70 euros, it will work for your purpose.




Auxin, i prefer working with monokarions, for various reasons:

- Once meiosis and spore production has take place , you are going to have a huge diversity in the F1 descendant as a result of recombination, so you can choose and select your desired traits. If you are looking for a specific trait, working with dykarions is like a lotery. example: You will never isolate a red mushroom if the region of the crhomosome involved has not recombined,even in 10th generations... you will be loosing your time. If you cross  monokarions you have much more gene pool, the "red trait" already IS in someplace, and you could isolate it by doing many generations

If you work with dykarions, you can?t know how much cells have recombined and how much recombination has occured. You may have products with a 0,000001% of recombination that will never show traits of one parental.

Another problem i see is that you may isolate a product with cells that have recombined and others that not.

Please, correct me if i?m wrong, that?s only my opinion





I agree. Monokarions are the way to go. I started out with Dykarions because thats all I have to work with at the moment.

Mushroom genetics are still not fully understood. There has been more work done with plants. Thats were most confusion takes place is because people compare mushroom genes to plant genes and they are not the same at all.


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