|
 phreedom
Stranger
Registered: 03/12/02
Posts: 357
Last seen: 8 years, 11 months
|
 Post deleted by MOE THE MAD SCIENTIST
#1107772 - 12/03/02 03:41 PM (9 years, 5 months ago) |
|
|
Edited by phreedom (12/03/02 04:19 PM)
|
 Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
|
 Re: agar didn't solidify [Re: phreedom]
#1108125 - 12/03/02 06:54 PM (9 years, 5 months ago) |
|
|
You didn't have a high enough agar/water ratio. How many grams of each agar did you add to what volume of water?
Joshua
--------------------
The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
Edited by Joshua (12/03/02 07:19 PM)
|
phreedom
Stranger
Registered: 03/12/02
Posts: 357
Last seen: 8 years, 11 months
|
 Post deleted by MOE THE MAD SCIENTIST [Re: Joshua]
#1108934 - 12/04/02 12:07 AM (9 years, 5 months ago) |
|
|
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,064
Loc: my room
Last seen: 7 hours, 36 minutes
|
Re: agar didn't solidify [Re: phreedom]
#1109044 - 12/04/02 01:03 AM (9 years, 5 months ago) |
|
|
> but i couldn't pc until a few days later.
You mean you mixed the preprepared agar and water, and then left it sitting for a few days?
That?s bad, bad....
Start anew.
|
stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 2 years, 2 months
|
Re: agar didn't solidify [Re: Anno]
#1109481 - 12/04/02 07:53 AM (9 years, 5 months ago) |
|
|
It is? I didnt know that  ..nice to know that before it actually happends..usually i do PC my agar right away though.[so far.]
Anno: Do you know why?[does AGAR loose it solidyfing properties when left sitting in water?hmm..?]
peace...
|
Raadt
nicht
Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 4 years, 4 months
|
|
oxidization? breakdown?
my guesses.
--------------------
Raadt
-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,064
Loc: my room
Last seen: 7 hours, 36 minutes
|
|
What you did was let a nourishing solution(agar agar, sugar(glucose, maltose) and yeast extract and water sit at room temperature.
I?m sure you got all sorts of nasty contaminats growing in the solution if you let it stand at room temperature for a few days....
You can let agar sit for a long time, but only if it?s sterilized before.
|
stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 2 years, 2 months
|
Re: agar didn't solidify [Re: Anno]
#1112827 - 12/05/02 05:49 AM (9 years, 5 months ago) |
|
|
Ok, i see that having an unsterilized highly nutrious mix sitting in room temp. for a couple of days cause contams..but do not sterilizing it cause that it?s not solidyifing when it is PC?d later on?
IE are the solydifing properties lost?
peace...
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,064
Loc: my room
Last seen: 7 hours, 36 minutes
|
|
agar has likely been digested by some bacteria, my guess.
|
EvilGir
Stranger
Registered: 11/26/01
Posts: 1,296
Loc: Planet Irk
Last seen: 2 years, 6 months
|
Re: agar didn't solidify [Re: Anno]
#1113560 - 12/05/02 10:37 AM (9 years, 5 months ago) |
|
|
Could of been contaminated by a bacteria and it byproduct could of increased the acidity of the agar which would stop it from solidifying.
--------------------
Think for your selves and question authority
|
|
Previous
Index
Next
Threaded
Shop for: 
Edit 
Reply 
