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InvisibleJef
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Agaricus augustus, Prince mushroom ,
    #10678618 - 07/15/09 12:50 AM (5 years, 4 months ago)

Has anyone cultured this one ?

All ideas appreciated.


Good growing.


--------------------
I am my own lab rat.


“Young lady!” cried Mr. Grout, very much appalled.  “It ill becomes a young person of no consequence, such as yourself, to go about slandering great people in all the dignity of their property!”
- Mrs. Mabb, The Ladies of Grace Adieu, by Susanna Clarke


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OfflineWorkmanV
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Re: Agaricus augustus, Prince mushroom , [Re: Jef]
    #10678628 - 07/15/09 12:52 AM (5 years, 4 months ago)



--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


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Invisiblenightflyer
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Re: Agaricus augustus, Prince mushroom , [Re: Workman]
    #10680374 - 07/15/09 10:38 AM (5 years, 4 months ago)

I have grown Agaricus augustus in a little flower pot.  (1 L substrate)
The substrate was a mixture of rotten conifer needles, grass, leaves,
sandy soil and 5% calcium carbonate, pasteurized.
Spawn was made of rye grass seed / barley grains (50/50).

You should start from a tissue culture.
Spore germination is VERY difficult.
Best agar medium: Oat flake agar
http://i521.photobucket.com/albums/w337/gerilori/Oatflakemedium.jpg

Delicious mushroom!



--------------------
PSILOCYBE  CYANOFRISCOSA - EUROPE 2009



http://www.shroomery.org/forums/showflat.php/Number/11179100


Edited by nightflyer (07/23/09 07:29 AM)


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OfflinePsilocelium
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Re: Agaricus augustus, Prince mushroom , [Re: nightflyer]
    #10690997 - 07/17/09 12:10 AM (5 years, 4 months ago)

I just researched that one last night. Ha. I was trying to ID a few I found in my friends front lawn.

The best I came up with is Agaricus Placomyces, too bad I didn't take a pic though. I have it on agar currently and will grow it out for the hell of it.


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InvisibleJef
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Re: Agaricus augustus, Prince mushroom , [Re: Workman]
    #10691378 - 07/17/09 01:15 AM (5 years, 4 months ago)

Thanks nightflyer for the account of your culture.  Never heard of OFA but I will try it.

Workman, cool photo spread, I didn't even know there were journals.

But what am I looking at ?

Does the mycelium look like stale rice pudding or maggots ?

or is that the shape of the grains it was covering ?



--------------------
I am my own lab rat.


“Young lady!” cried Mr. Grout, very much appalled.  “It ill becomes a young person of no consequence, such as yourself, to go about slandering great people in all the dignity of their property!”
- Mrs. Mabb, The Ladies of Grace Adieu, by Susanna Clarke


Edited by Jef (07/17/09 01:17 AM)


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Invisiblenightflyer
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Re: Agaricus augustus, Prince mushroom , [Re: Jef]
    #10692000 - 07/17/09 03:36 AM (5 years, 4 months ago)

Quote:

Jef said:  Never heard of OFA but I will try it.









Additional information about oat flake agar.
After cooking the oat flakes, filter the liquid using a gauze or a
metal strainer with a tight mesh and dilute the liquid to 1 L.
Beware, this medium will be less firm than other agar media,
so extra agar has to be added to compensate. (30gr. / L)

Here you find a lot of agar recipes, including oat agars:

http://www.shroomery.org/9393/FastFreds-Media-Cookbook


--------------------
PSILOCYBE  CYANOFRISCOSA - EUROPE 2009



http://www.shroomery.org/forums/showflat.php/Number/11179100


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InvisibleJef
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Re: Agaricus augustus, Prince mushroom , [Re: nightflyer]
    #10692005 - 07/17/09 03:37 AM (5 years, 4 months ago)

Thanks again.

Is there a problem with leaving the oats in ?


--------------------
I am my own lab rat.


“Young lady!” cried Mr. Grout, very much appalled.  “It ill becomes a young person of no consequence, such as yourself, to go about slandering great people in all the dignity of their property!”
- Mrs. Mabb, The Ladies of Grace Adieu, by Susanna Clarke


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Invisiblenightflyer
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Re: Agaricus augustus, Prince mushroom , [Re: Jef]
    #10692049 - 07/17/09 03:53 AM (5 years, 4 months ago)

I don't know. I always filter the liquid.
But I think it should be no problem.


--------------------
PSILOCYBE  CYANOFRISCOSA - EUROPE 2009



http://www.shroomery.org/forums/showflat.php/Number/11179100


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OfflineParesthesia
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Re: Agaricus augustus, Prince mushroom , [Re: nightflyer]
    #10692533 - 07/17/09 07:58 AM (5 years, 4 months ago)

The other day I mixed some agar using the following procedure:

1. Added fermented corn stalks, and cottonseed hulls (waste substrate from a paddy straw mushroom experiment) to a pot of boiling water and steeped for an hour.  The resulting liquid was dark from the soil on the corn roots, and it smelled like... corn stalks!
2. Strained corn liquid through a few paper towels.
3. Added 10g of corn dextrose and 20g of agar to 500ml of the liquid and 500ml of water.
4. Whipped up mix on my stir plate, PC'd and poured up plates.

Is it the live bacteria that aid in Agaricus spore germination, or is it the bacterial effluent? I have a spore print from a meadow mushroom I found last fall, and a couple of cultures from secondary decomposers.  (Agaricus bitorquis, Coprinus comatus and Chlorophyllum rhacodes -- all of these have grown very slowly on MEA or PDA, and my culture attempts have met with failure.)


--------------------
"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."

- T. S. Eliot

I'm currently looking for cultures of the following species:

Calocybe indica, Chlorophyllum rachodes, Lentinula boryana, Polyporus umbellatus


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Invisiblenightflyer
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Re: Agaricus augustus, Prince mushroom , [Re: Paresthesia]
    #10692717 - 07/17/09 09:27 AM (5 years, 4 months ago)

Agaricus bitorquis and Coprinus comatus should grow fine
on MEA or PDA. The pH-value of the agar should be 4.5

Here is a helpful paper about spore germination and tissue culture:

http://i521.photobucket.com/albums/w337/gerilori/Paper.jpg
http://i521.photobucket.com/albums/w337/gerilori/Paper1.jpg
http://i521.photobucket.com/albums/w337/gerilori/Paper2.jpg


--------------------
PSILOCYBE  CYANOFRISCOSA - EUROPE 2009



http://www.shroomery.org/forums/showflat.php/Number/11179100


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InvisibleJef
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Re: Agaricus augustus, Prince mushroom , [Re: nightflyer]
    #10694086 - 07/17/09 02:56 PM (5 years, 4 months ago)

Wow.  THis has become a better thread than I started !

:smile:
Very cool about germinating above a related species on cellophane and allowing some contams.

I tried to culture some bacteria that my pleurotus loved, but it became contaminated....with pleurotus!


--------------------
I am my own lab rat.


“Young lady!” cried Mr. Grout, very much appalled.  “It ill becomes a young person of no consequence, such as yourself, to go about slandering great people in all the dignity of their property!”
- Mrs. Mabb, The Ladies of Grace Adieu, by Susanna Clarke


Edited by Jef (07/17/09 02:58 PM)


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Invisiblenightflyer
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Re: Agaricus augustus, Prince mushroom , [Re: Jef]
    #10694428 - 07/17/09 04:07 PM (5 years, 4 months ago)

Quote:

Jef said:


I tried to culture some bacteria that my pleurotus loved, but it became contaminated....with pleurotus!





--------------------
PSILOCYBE  CYANOFRISCOSA - EUROPE 2009



http://www.shroomery.org/forums/showflat.php/Number/11179100


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Offlinequadracer
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Re: Agaricus augustus, Prince mushroom , [Re: nightflyer]
    #10695852 - 07/17/09 08:09 PM (5 years, 4 months ago)

dang this is awesome, especially considering A. augustus is the only mushroom popping up in my area these days.

Then again, I am a total newb when it comes to mushrooms growing, but I am always down for a challenge.


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InvisibleJef
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Re: Agaricus augustus, Prince mushroom , [Re: quadracer]
    #10695956 - 07/17/09 08:31 PM (5 years, 4 months ago)

This is one of very few mushrooms to fruit at very dry times of year.

In my area, first flush beginning to mid June, often another flush three weeks later and then one more in the early fall.

This is a delicious mushroom with a strong smell and taste of mushroom with almonds.


--------------------
I am my own lab rat.


“Young lady!” cried Mr. Grout, very much appalled.  “It ill becomes a young person of no consequence, such as yourself, to go about slandering great people in all the dignity of their property!”
- Mrs. Mabb, The Ladies of Grace Adieu, by Susanna Clarke


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Invisiblenightflyer
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Registered: 08/08/08
Posts: 392
Loc: Central Europe
Re: Agaricus augustus, Prince mushroom , [Re: Jef]
    #10697503 - 07/18/09 02:09 AM (5 years, 4 months ago)

Quote:

Jef said:
This is one of very few mushrooms to fruit at very dry times of year.





Dry times? That's funny!
Here in Central Europe, we have rain every week since
the beginning of June and summer mushrooms are poppin' up
like crazy. Boletus aestivalis everywhere and the lawns are
full of Marasmius oreades.


--------------------
PSILOCYBE  CYANOFRISCOSA - EUROPE 2009



http://www.shroomery.org/forums/showflat.php/Number/11179100


Edited by nightflyer (07/18/09 02:18 AM)


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Offlinecutemushie
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Re: Agaricus augustus, Prince mushroom , [Re: Paresthesia]
    #10697793 - 07/18/09 03:33 AM (5 years, 4 months ago)

Quote:

Paresthesia said:
The other day I mixed some agar using the following procedure:

1. Added fermented corn stalks, and cottonseed hulls (waste substrate from a paddy straw mushroom experiment) to a pot of boiling water and steeped for an hour.  The resulting liquid was dark from the soil on the corn roots, and it smelled like... corn stalks!
2. Strained corn liquid through a few paper towels.
3. Added 10g of corn dextrose and 20g of agar to 500ml of the liquid and 500ml of water.
4. Whipped up mix on my stir plate, PC'd and poured up plates.

Is it the live bacteria that aid in Agaricus spore germination, or is it the bacterial effluent? I have a spore print from a meadow mushroom I found last fall, and a couple of cultures from secondary decomposers.  (Agaricus bitorquis, Coprinus comatus and Chlorophyllum rhacodes -- all of these have grown very slowly on MEA or PDA, and my culture attempts have met with failure.)




Last year I had a wild clone of Lepista nuda and it died on agar.  I don't know if it was bacteria contamination or that it died because it needed some bacteria.  I have a feeling that it needs bacteria.  The mycelium was growing into the agar instead of the normal surface growth.  Found one specimen yesterday and am cloning it.  Wonder if there's a way to culture those spp that require bacteria on agar.  I know many of them just grow very slowly and eventually dying off eg Agaricus spp.  Maybe pasteurizing agar instead of sterilizing?  Any suggestions?


--------------------
HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa.

LOOKING FOR: Those that I don't have.


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Invisiblenightflyer
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Re: Agaricus augustus, Prince mushroom , [Re: cutemushie]
    #10697866 - 07/18/09 04:16 AM (5 years, 4 months ago)

Quote:

cutemushie said:
  The mycelium was growing into the agar instead of the normal surface growth.




That is called "submers growth" and it's a hint that the
agar medium is too rich in nutrients.. Try Malt Extract Agar (2%)
plus 2% yeast or oat flake agar. Adjust pH value to 4.5
Use 30gr. agar / 1 L  for oat flake medium.
http://i521.photobucket.com/albums/w337/gerilori/Oatflakemedium.jpg
Bacteria are not necessary to start a tissue culture (clone)
of Lepista nuda or Agaricus spp. but they could be beneficial
if you germinate spores.

Cultivation of Australian strains of Lepista nuda:
http://www.hort.purdue.edu/newcrop/proceedings1996/v3-476.html


--------------------
PSILOCYBE  CYANOFRISCOSA - EUROPE 2009



http://www.shroomery.org/forums/showflat.php/Number/11179100


Edited by nightflyer (07/18/09 07:57 AM)


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Offlinekaste
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Re: Agaricus augustus, Prince mushroom , [Re: nightflyer]
    #10698593 - 07/18/09 11:15 AM (5 years, 4 months ago)

Nice ones :smile:


--------------------


It could be worse. A woman could cut off your penis while you're sleeping and toss it out the window of a moving car.


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Offlinecutemushie
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Re: Agaricus augustus, Prince mushroom , [Re: nightflyer]
    #10709608 - 07/20/09 03:50 PM (5 years, 4 months ago)

Quote:

nightflyer said:
Quote:

cutemushie said:
  The mycelium was growing into the agar instead of the normal surface growth.




That is called "submers growth" and it's a hint that the
agar medium is too rich in nutrients.. Try Malt Extract Agar (2%)
plus 2% yeast or oat flake agar. Adjust pH value to 4.5
Use 30gr. agar / 1 L  for oat flake medium.
http://i521.photobucket.com/albums/w337/gerilori/Oatflakemedium.jpg
Bacteria are not necessary to start a tissue culture (clone)
of Lepista nuda or Agaricus spp. but they could be beneficial
if you germinate spores.

Cultivation of Australian strains of Lepista nuda:
http://www.hort.purdue.edu/newcrop/proceedings1996/v3-476.html




Hi Nightflyer,

Thanks for the info.  I was using 2% malt agar when growing it.  I always used 2% for all my cultures, never liked 4%.  Somehow I find it way too rich for my mushrooms.  Maybe I'll use 1% for Lepista nuda and see what happens. 

What is the yeast for?  I see people adding it all the time and I'm thinking if the benefits are not too great, I won't add it (because it is one more thing I have to measure out and add.  I'm very lazy.)

2 days ago I went back to the same spot and found the first (and only one) Lepista nuda for the season.  A bit late for it because of the dry environment and diminishing substrate, and old.  Anyhow, I took it and am cloning it.

I also found and cloned an Agaricus augustus from another site.

Thanks for the link.  I'm reading it now.

Cheers,
cutemushie


--------------------
HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa.

LOOKING FOR: Those that I don't have.


Edited by cutemushie (07/20/09 03:57 PM)


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Invisiblenightflyer
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Re: Agaricus augustus, Prince mushroom , [Re: cutemushie]
    #10713726 - 07/21/09 10:14 AM (5 years, 4 months ago)

Quote:

cutemushie said:


What is the yeast for? 




Shrooms need Vitamin B1 and yeast is rich in Vitamin B1 (Thiamine).
But most fungi are able to synthesize it. It's not that important.


--------------------
PSILOCYBE  CYANOFRISCOSA - EUROPE 2009



http://www.shroomery.org/forums/showflat.php/Number/11179100


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