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 stveo77
novice
Registered: 01/04/09
Posts: 17
Last seen: 2 years, 10 months
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 always getting contamed???
#10615500 - 07/03/09 11:18 AM (2 years, 10 months ago) |
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i spray the room dwn with lysol. wipe wipe ,everything..and i dnt open door till done.. im still getting pretty much all contamed with green mold..i dnt understand, i sprayed the whole room and air. any suugestions
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kaneabel
getting my feet wet
Registered: 01/10/05
Posts: 133
Loc: Central Indiana
Last seen: 2 years, 5 months
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Re: always getting contamed??? [Re: stveo77]
#10615517 - 07/03/09 11:23 AM (2 years, 10 months ago) |
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look at other prep methods, how clean are you when you prepare your syringe, jars, etc?
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 1 year, 13 days
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Re: always getting contamed??? [Re: stveo77]
#10615552 - 07/03/09 11:32 AM (2 years, 10 months ago) |
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Learn sterile culture technique. It isn't at all as easy as you thing you have to be methodical. You have a glovebox? a simple cardboard box with a sheet of plastic over the front works better than nothing sometimes. Usually one overlooks something so obvious. Every surface must be wiped down properly from containers to instruments to the surface your working on. If you set something down it needs to be wiped again before implementing.
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal 
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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electrics
wave rider
Registered: 12/02/08
Posts: 1,513
Loc: In front of my Computer
Last seen: 2 days, 3 hours
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Re: always getting contamed??? [Re: kaneabel]
#10615558 - 07/03/09 11:34 AM (2 years, 10 months ago) |
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Yes somehow you're missing something either a PC or use new substrate material..A good glovebox is a must also reliable spores If you don't have a good print to work from that could be you're problem....Contams are really easily avoided if you folow tek's to the T hope this helps good luck in the future.........e
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"Listen now I'm talking I've been here for weeks, waiting in this growing crowd staring at my feet, The world around me is Turning I'm just standing still, The time has come for changes do something or I will" Phish
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 1 year, 13 days
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Re: always getting contamed??? [Re: electrics]
#10615918 - 07/03/09 12:44 PM (2 years, 10 months ago) |
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Quote:
electrics said:
Yes somehow you're missing something either a PC or use new substrate material..A good glovebox is a must also reliable spores If you don't have a good print to work from that could be you're problem....Contams are really easily avoided if you folow tek's to the T hope this helps good luck in the future.........e
Good spores! 
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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psily
Registered: 02/11/09
Posts: 116
Last seen: 1 year, 1 month
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Re: always getting contamed??? [Re: hyphae]
#10617554 - 07/03/09 06:31 PM (2 years, 10 months ago) |
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Maybe you don't sterilize your jars long enough?
I ask, because my last 3 jars got contammed super-green, and the only difference with the rest was that I PC'ed those 3 jars last (it's a small PC and I had to do 3 batches that day). Those contams were due to insufficient PC time.
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All mushrooms are edible but some are edible only once. 
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 BUDDHA_702
Master Mycologist In Training
Registered: 02/17/07
Posts: 1,296
Loc: Some Country
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Re: always getting contamed??? [Re: psily]
#10617571 - 07/03/09 06:35 PM (2 years, 10 months ago) |
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PF tek?
do you use a glove box?
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tripdawg420
low life with no life
Registered: 02/02/09
Posts: 1,333
Loc: illinois
Last seen: 53 minutes, 10 seconds
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Re: always getting contamed??? [Re: BUDDHA_702]
#10617588 - 07/03/09 06:39 PM (2 years, 10 months ago) |
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do u flame yur needle
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MushroomMaster
Horticulturalist
Registered: 07/02/09
Posts: 59
Loc: Deep Sea Lair
Last seen: 2 years, 10 months
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Re: always getting con tamed??? [Re: tripdawg420]
#10618020 - 07/03/09 08:16 PM (2 years, 10 months ago) |
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Make yourself a homemade glove box using a clear rubber tote.
Spray non scented Lysol into the glove box and let settle with tools and jars.
Put on some glove and flame the needle red hot and your golden.
I haven't contamed in a long time.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 36,989
Loc: Colorado 
Last seen: 6 hours, 20 minutes
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What kind of jars are you shooting up? How are you prepping them?
A glovebox is nice, but it's not totally necessary. But good sterile materials are.
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MushroomMaster
Horticulturalist
Registered: 07/02/09
Posts: 59
Loc: Deep Sea Lair
Last seen: 2 years, 10 months
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Re: always getting con tamed??? [Re: Doc_T]
#10618039 - 07/03/09 08:24 PM (2 years, 10 months ago) |
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Sorry man but most of us would agree that its totally necessary, It's not a smart move to work with out it. Its cheap, you can buy one for around 6$. Pretty cheap. A flow hood would be great but more expensive.
If your not using a pressure cooker then I would recomend saving your money. 60$ Walmart. Pressure cookers do 90% of the sterilizing. The other 10% is all you.
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JMath
Stranger
Registered: 06/06/09
Posts: 120
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I'm only on my first grow but I feel like you don't absolutely need to use a glovebox. The alternative to using one would be doing the inoculation in the cleanest room of your house (doesn't matter where, but kitchens and bathrooms are bad). Turn off the air conditioning/heating system, shut the door, clean all of your utensils, have everything ready to go, then just don't move for a good five minutes or so to let the air settle.
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It's only after we've lost everything that we're free to do anything.
-Chuck Palahniuk
Trying to catch the impossible dream
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,068
Loc: All The Colors
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Re: always getting con tamed??? [Re: JMath]
#10618170 - 07/03/09 08:59 PM (2 years, 10 months ago) |
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Quote:
I'm only on my first grow but I feel like you don't absolutely need to use a glovebox.
If you're just on your first grow don't just come up with theories that go against people with thousends of grows. What do you know about it? A bathroom is the best place to do it if you clean it up well. There's hardly any wood and there are no carpets or if there are you can take them out. I spray everything with bleach, wipe everything with alcohol, use a glovebox, shower, brush teet, wear gloves, scrubs, head net...
Edit: I just wish I had a flow hood...
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
Edited by AlexP (07/03/09 09:00 PM)
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proudabu
creeper
Registered: 12/05/08
Posts: 615
Loc: Under My Bed
Last seen: 4 months, 10 days
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Re: always getting con tamed??? [Re: JMath]
#10618178 - 07/03/09 09:01 PM (2 years, 10 months ago) |
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i personally use a small bathroom. l close the toilet seat. whipe counter with lysol cover vents and spray lysol all into the room everywhere then shut it let it sit and come back in. never had a problem with contams i also use a pressure cooker though.
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MushroomMaster
Horticulturalist
Registered: 07/02/09
Posts: 59
Loc: Deep Sea Lair
Last seen: 2 years, 10 months
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Re: always getting con tamed??? [Re: JMath]
#10618181 - 07/03/09 09:01 PM (2 years, 10 months ago) |
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You have to understand that their are all kinds of micro organisms floating all around, even walking stirs them all up. You may get lucky but why risk it? You spent your cash to get this far whats 6 more?
Just make it simple
Lysol the air and let it settle.
Cut two holes and wear gloves.
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tazz
the spazz
Registered: 04/21/09
Posts: 162
Loc: The Great White North
Last seen: 11 months, 12 days
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Re: always getting con tamed??? [Re: JMath]
#10618183 - 07/03/09 09:02 PM (2 years, 10 months ago) |
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Quote:
JMath said:
I'm only on my first grow but I feel like you don't absolutely need to use a glovebox.
You don't absolutely need to, but why wouldn't you? They're dead easy to make and can be made for ultra cheap. So why not do it and help eliminate potential problems?
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The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,054
Loc: Texas
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Re: always getting con tamed??? [Re: JMath]
#10618185 - 07/03/09 09:02 PM (2 years, 10 months ago) |
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Quote:
JMath said:
I'm only on my first grow but I feel like you don't absolutely need to use a glovebox. The alternative to using one would be doing the inoculation in the cleanest room of your house (doesn't matter where, but kitchens and bathrooms are bad). Turn off the air conditioning/heating system, shut the door, clean all of your utensils, have everything ready to go, then just don't move for a good five minutes or so to let the air settle.
Not to be mean but for a person on their first grow, I wouldn't be giving that advice... Quote:
you don't absolutely need to use a glovebox
. On the other hand, you did offer some great advice.... Quote:
Turn off the air conditioning/heating system,
. That is something important that most people miss. If the OP has had zero success, then you shouldn't cut ANY corners. A glove box is simple to make, inexpensive and absolutely indispensable tool for a beginning mycologist.
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CH HELL
Brain Sturgeon
Registered: 10/02/08
Posts: 5,387
Loc: mars
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If you are using your bathroom then move to your dining room. To many germs in the bathroom. Some people get away with using the bathroom but I wouldn't risk it.
GL,
CH
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,068
Loc: All The Colors
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Re: always getting con tamed??? [Re: CH HELL]
#10618256 - 07/03/09 09:26 PM (2 years, 10 months ago) |
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I use my bedroom, love the smell of bleach!
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 1 year, 13 days
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Quote:
MushroomMaster said:
You have to understand that their are all kinds of micro organisms floating all around, even walking stirs them all up. You may get lucky but why risk it? You spent your cash to get this far whats 6 more?
Just make it simple
Lysol the air and let it settle.
Cut two holes and wear gloves.
Perfect!
--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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