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 Leadwing
King in my own Mind
 
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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 Jars not colonising :( 
#10610438 - 07/02/09 03:22 PM (4 months, 18 days ago) |
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I inoculated my jars about a week ago now, and still don't see anything!
I sterilised them, did everything properly, have kept them in a plastic container at the right temp...
Wahhh :'(
What could the reason be?
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Grab every second given to you and make love to it.
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Lostkeys
Fractal Thought Pattern
Registered: 06/06/09
Posts: 784
Loc: Sirius
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Re: Jars not colonising :( [Re: Leadwing]
#10610450 - 07/02/09 03:25 PM (4 months, 18 days ago) |
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More information is required. What type of jars? Are your inoculation holes covered? What temperature are you colonizing at?
--------------------
"And i think it's gonna be a long long time
Till touch down brings me round again to find
I'm not the man they think i am at home
Oh no no no i'm a rocket man
Rocket man burning out his fuse up here alone"
Everything posted in this forum is fictional, only a fool would take the things written here as fact.
For real time cultivation chat go here and type /join #cultivation
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Devii
Curious Examiner
Registered: 05/11/09
Posts: 189
Last seen: 6 days, 21 hours
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Re: Jars not colonising :( [Re: Leadwing]
#10610455 - 07/02/09 03:25 PM (4 months, 18 days ago) |
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Where did you get your spores from? They might just be bunk spores...
But more likely, did you allow your jars to cool for 24 hours after you PC'd them? If you inoculate a hot jar, the temperature will kill your spores.
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Psuper
W-W-Wyzzerdd
Registered: 02/12/08
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Loc: PNW (where else?)
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Re: Jars not colonising :( [Re: Leadwing]
#10610462 - 07/02/09 03:26 PM (4 months, 18 days ago) |
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Grains or cakes? What kind of filter? What temps? How many jars? Is their excessive moisture?
You don't need them in a plastic tub. Can you open a jar and smell it? A sour or old sock smell is a sign of bacteria. No signs of growth in 7days means something is wrong. ~Pixie~
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<------is looking to find a morel patch this year. Maybe smoke-drying some of them. Only found False morels last year.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610760 - 07/02/09 04:31 PM (4 months, 18 days ago) |
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4 jars
2 part Verm, 1 part BRF, 1 part water
Around 26C
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Grab every second given to you and make love to it.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610771 - 07/02/09 04:33 PM (4 months, 18 days ago) |
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I covered the jars (each with a hole in) with 2-3 layers of foil, secured it tightly.
I didn't cover up the inoculation holes after I inoculated them, as it had dry Verm ontop.
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Grab every second given to you and make love to it.
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 AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10610812 - 07/02/09 04:40 PM (4 months, 18 days ago) |
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Quote:
I covered the jars (each with a hole in) with 2-3 layers of foil, secured it tightly.
There's no gas exchange that way. Dry verm on top of the inoc holes? Never heard of anything like that...
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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blazeup420
?got issues?
Registered: 04/30/09
Posts: 133
Last seen: 1 month, 7 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610816 - 07/02/09 04:41 PM (4 months, 18 days ago) |
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ive had the same problem twice still cant figure it out...is your BRF organic?mines not but that shouldnt matter...but ya i need to figure this out to...
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 I  LOVE  MY  DRUGS 
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: AlexP]
#10610823 - 07/02/09 04:42 PM (4 months, 18 days ago) |
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No, I mean the jars are 3/4 full of mix, and the last 1/4 is dry Verm.
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Grab every second given to you and make love to it.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610833 - 07/02/09 04:43 PM (4 months, 18 days ago) |
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Also... gas exchange?
Surely they need to be pretty much air tight so theres no chance of contams?
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Grab every second given to you and make love to it.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10610854 - 07/02/09 04:47 PM (4 months, 18 days ago) |
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Quote:
Surely they need to be pretty much air tight so theres no chance of contams?
Air tight jars will stall. Haven't you watched the videos? What are you doing?... What are you following?... Remove that foil and what type of filter?... Who do the lids look like? The verm layer is there to stop contams from attacking your mycelium or substrate.
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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kaneabel
getting my feet wet
Registered: 01/10/05
Posts: 133
Loc: Central Indiana
Last seen: 16 days, 23 hours
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Re: Jars not colonising :( [Re: Leadwing]
#10610860 - 07/02/09 04:48 PM (4 months, 18 days ago) |
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The verm acts as a filter for the air, it should exchange air ok, sometimes near the end of colonization if it seems like it is stalling, you can turn them upside down and what happens is when you do that the mass of everything comes down and creates a vacuum effect and brings more oxygen inside the jar to finish up.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610888 - 07/02/09 04:53 PM (4 months, 18 days ago) |
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Guide
I must admit, I sort of rushed things...
I still have half a syringe left, might have another go.
Also, is it normal that the syringe had clumps in it? It looked clear apart from the clumps. (I shook it up as much as poss)
The jars I used have screw on tops
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Grab every second given to you and make love to it.
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DWreck
Killin it
Registered: 03/03/09
Posts: 180
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Re: Jars not colonising :( [Re: Leadwing]
#10610897 - 07/02/09 04:55 PM (4 months, 18 days ago) |
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well first thing take that foil off..its only used to keep out the water while p/cing. how many holes in your lid? how deep is your dry verm layer? But honestly, It sounds like you need to go watch rr's videos.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: kaneabel]
#10610901 - 07/02/09 04:56 PM (4 months, 18 days ago) |
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Quote:
you can turn them upside down and what happens is when you do that the mass of everything comes down and creates a vacuum effect and brings more oxygen inside the jar to finish up.
"Don't flip jars. As prisoner said, it screws up the vermiculite filter. CO2 doesn't need to 'drain' out by flipping the jar upside down. That's a bit of disinformation that once typed refuses to die. The reason is the heat produced by the mycelium causes circulation that takes care of the gas exchange." RR
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: AlexP]
#10610913 - 07/02/09 04:58 PM (4 months, 18 days ago) |
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--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610916 - 07/02/09 04:58 PM (4 months, 18 days ago) |
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Just the one (tiny) hole... I'll go take the foil off
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Grab every second given to you and make love to it.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10610929 - 07/02/09 05:00 PM (4 months, 18 days ago) |
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And loosen up the lids. 
Edit: And watch the videos!!! Watch a professional do the work and then follow!
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
Edited by AlexP (07/02/09 05:01 PM)
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DWreck
Killin it
Registered: 03/03/09
Posts: 180
Loc: Maine
Last seen: 36 minutes, 40 seconds
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Re: Jars not colonising :( [Re: Leadwing]
#10610952 - 07/02/09 05:04 PM (4 months, 18 days ago) |
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There's supposed to be 4 holes. You definitely need to check out the link Alex posted. if you follow that to the t You will succeed
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610966 - 07/02/09 05:06 PM (4 months, 18 days ago) |
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Oh holy crap wicked, I think there's some signs of life at the bottom of 2 of the jars! WOOOOOT
Also will the same syringe be good to use again? Its like 2/3 full
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Grab every second given to you and make love to it.
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proudabu
creeper
Registered: 12/05/08
Posts: 435
Loc: Under My Bed
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Re: Jars not colonising :( [Re: AlexP]
#10610971 - 07/02/09 05:07 PM (4 months, 18 days ago) |
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there should be four holes when making cakes to inject in four different spots and i usually put cloth band aids over mine. dont quote me but what would somebody else recommend covering it with a coffee filter and rubber banding it after your done? just an idea i really never did cakes so its not my expertise
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: proudabu]
#10610991 - 07/02/09 05:10 PM (4 months, 18 days ago) |
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A coffee filter is quite useless for keeping contams out. The verm layer is for keeping contams out.
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: AlexP]
#10611027 - 07/02/09 05:16 PM (4 months, 18 days ago) |
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If you guys want to learn something, this is the link to follow.
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,225
Loc: the rain forests
Last seen: 2 months, 28 days
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Re: Jars not colonising :( [Re: AlexP]
#10611058 - 07/02/09 05:20 PM (4 months, 18 days ago) |
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Quote:
AlexP said:
Quote:
you can turn them upside down and what happens is when you do that the mass of everything comes down and creates a vacuum effect and brings more oxygen inside the jar to finish up.
"Don't flip jars. As prisoner said, it screws up the vermiculite filter. CO2 doesn't need to 'drain' out by flipping the jar upside down. That's a bit of disinformation that once typed refuses to die. The reason is the heat produced by the mycelium causes circulation that takes care of the gas exchange." RR
Bullshit! This is a tried and true method to renew growth to stalled jars! Also it's not for CO2 drainage as you said it simply allows the cake to settle this give a brief exchange in gas. I don't give a shit what someone thinks they know if it works and has worked thousands of times you can't dis it!!! It don't do shit to the verm layer that is pure crap!!!! Unless someone doesn't have a clue and tries to shake the damn jar then they deserve what they get. Myc generates heat regardless if the cake is right side up or upside down and that doesn't always take care of gas exchange it simply pushes out gas from the top of the culture.
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal 
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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DWreck
Killin it
Registered: 03/03/09
Posts: 180
Loc: Maine
Last seen: 36 minutes, 40 seconds
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Re: Jars not colonising :( [Re: AlexP]
#10611061 - 07/02/09 05:20 PM (4 months, 18 days ago) |
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yea dont cover it. Just take the foil off and losen the lid to provide g/e. was the one whole in the center? Oh and your left over spores should still be good. Just be sure to flame sterilize. Are you gonna watch the vids?
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: DWreck]
#10611120 - 07/02/09 05:33 PM (4 months, 18 days ago) |
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Yeah I'll watch the vids for next time I grow
The hole is in the center, I've removed the foil, loosened the lids, and put them back.
I see myc in 2 of them at the bottom (although one of them has some wet bits...) 
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Grab every second given to you and make love to it.
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Psuper
W-W-Wyzzerdd
Registered: 02/12/08
Posts: 2,110
Loc: PNW (where else?)
Last seen: 5 hours, 1 minute
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Re: Jars not colonising :( [Re: DWreck]
#10611158 - 07/02/09 05:41 PM (4 months, 18 days ago) |
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If your syringe has clumps in it, go ahead and dilute it. You want to be inoculating with fairly "light" syringes anyway. A syringe with clumps of spores can make a handful of syringes.
If you're really low, you just use less inoculant. I inoculated a quart of grain with less than 1/4cc; just colonizes a little slower. ~Pixie~
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<------is looking to find a morel patch this year. Maybe smoke-drying some of them. Only found False morels last year.
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 guest1
Mycena
Registered: 05/25/09
Posts: 61
Last seen: 49 seconds
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Re: Jars not colonising :( [Re: Psuper]
#10611210 - 07/02/09 05:50 PM (4 months, 18 days ago) |
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give it 5 weeks. I have seen spores take 5 weeks to show first signs. People are too anxious and after a few minutes or days after inoculation, they panic and think it is a failure. just sit back, as long as there are no contams, let it go.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: guest1]
#10611237 - 07/02/09 05:54 PM (4 months, 18 days ago) |
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Quote:
give it 5 weeks. I have seen spores take 5 weeks to show first signs.
Huummm? Taking too much space for me! I dump them out if they don't show growth for a week in the right conditions.
--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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Lostkeys
Fractal Thought Pattern
Registered: 06/06/09
Posts: 784
Loc: Sirius
Last seen: 7 days, 1 hour
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Re: Jars not colonising :( [Re: guest1]
#10611471 - 07/02/09 06:35 PM (4 months, 18 days ago) |
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Yeah five weeks is a bit excessive considering my jars showed myc growth on the third day...
--------------------
"And i think it's gonna be a long long time
Till touch down brings me round again to find
I'm not the man they think i am at home
Oh no no no i'm a rocket man
Rocket man burning out his fuse up here alone"
Everything posted in this forum is fictional, only a fool would take the things written here as fact.
For real time cultivation chat go here and type /join #cultivation
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,225
Loc: the rain forests
Last seen: 2 months, 28 days
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Re: Jars not colonising :( [Re: Lostkeys]
#10611500 - 07/02/09 06:41 PM (4 months, 17 days ago) |
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Longest I've waited was three weeks and that was two weeks of hell! I've got patience but not that much!!!
--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Lucid_Euphoria
Dualist
Registered: 03/23/05
Posts: 638
Loc: Flipside
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Re: Jars not colonising :( [Re: Leadwing]
#10611519 - 07/02/09 06:46 PM (4 months, 17 days ago) |
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Quote:
Also will the same syringe be good to use again? Its like 2/3 full
Yes, just be sure to flame sterilize the needle before inoculation.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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I flame sterilized the needle when I did it the first time, I heard popping - the sound of spores dying??? :'(
Also the needle turned kinda black so I cleaned it off / cooled it with a Dettol wipe, is that ok?
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Grab every second given to you and make love to it.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10614198 - 07/03/09 06:13 AM (4 months, 17 days ago) |
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Sorry the stickers are in the way lol
 
Not the ideal shape of jar I guess
Hole in the top
My jars 
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Grab every second given to you and make love to it.
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Lostkeys
Fractal Thought Pattern
Registered: 06/06/09
Posts: 784
Loc: Sirius
Last seen: 7 days, 1 hour
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Re: Jars not colonising :( [Re: Leadwing]
#10614558 - 07/03/09 08:11 AM (4 months, 17 days ago) |
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Seriously man, watch RogerRabbit's BRF Tek Videos. You won't be sorry, I promise...
--------------------
"And i think it's gonna be a long long time
Till touch down brings me round again to find
I'm not the man they think i am at home
Oh no no no i'm a rocket man
Rocket man burning out his fuse up here alone"
Everything posted in this forum is fictional, only a fool would take the things written here as fact.
For real time cultivation chat go here and type /join #cultivation
|
Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Lostkeys]
#10614621 - 07/03/09 08:36 AM (4 months, 17 days ago) |
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Watching now
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Grab every second given to you and make love to it.
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JMath
Registered: 06/06/09
Posts: 117
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Re: Jars not colonising :( [Re: Leadwing]
#10614781 - 07/03/09 09:15 AM (4 months, 17 days ago) |
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My syringe made popping noises too when I first inoculated, I think it's just from verm that gets stuck up in the needle and heats up and shoots out.
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It's only after we've lost everything that we're free to do anything.
-Chuck Palahniuk
Trying to catch the impossible dream
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,225
Loc: the rain forests
Last seen: 2 months, 28 days
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Re: Jars not colonising :( [Re: JMath]
#10614934 - 07/03/09 09:50 AM (4 months, 17 days ago) |
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It's the solution (spore water) in the needle most of the time.
--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: hyphae]
#10615187 - 07/03/09 10:40 AM (4 months, 17 days ago) |
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When the spore water at room temperature hits the metal flame sterilized syringe it makes popping noises!
--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: AlexP]
#10619092 - 07/04/09 03:45 AM (4 months, 16 days ago) |
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Also, I put the syringe back in its plastic bag, in a tube, and in a room temp drawer, that's ok yesh?
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Grab every second given to you and make love to it.
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rizingfire
Mycoticus psychoticus
Registered: 09/10/07
Posts: 630
Loc: North-east USm'f'nA
Last seen: 10 hours, 38 minutes
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Re: Jars not colonising :( [Re: Leadwing]
#10619167 - 07/04/09 04:47 AM (4 months, 16 days ago) |
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Ya you can use the syringe again just flame the tip or clean with alcohol....don't re-inoculate them...just be patient...
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10619607 - 07/04/09 07:53 AM (4 months, 16 days ago) |
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Yes, the spore syringe can be kept at room temperature.
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,225
Loc: the rain forests
Last seen: 2 months, 28 days
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Re: Jars not colonising :( [Re: Leadwing]
#10619834 - 07/04/09 09:01 AM (4 months, 16 days ago) |
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Quote:
Leadwing said:
Also, I put the syringe back in its plastic bag, in a tube, and in a room temp drawer, that's ok yesh?
That will be just fine.
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: hyphae]
#10621369 - 07/04/09 02:45 PM (4 months, 16 days ago) |
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Update:
ALL FOUR have signs of growth, yay! 
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Grab every second given to you and make love to it.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10621402 - 07/04/09 02:55 PM (4 months, 16 days ago) |
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Now wait till full colonization and THEN wait ANOTHER week.
After that you may dunk and roll.
GL
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: AlexP]
#10621475 - 07/04/09 03:16 PM (4 months, 16 days ago) |
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Do I have to dunk and roll?
I watched the video and it looks well brutal!!
Does it not increase risk of contam?
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Grab every second given to you and make love to it.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10621483 - 07/04/09 03:19 PM (4 months, 16 days ago) |
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No way. It's great that you do it. Cakes are very resistant to contams after full colonization. You watched the videos? That's great! Any question you might have just shoot!
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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hammy
Me, Myself and I
Registered: 06/17/09
Posts: 257
Loc: Greater Seattle Area
Last seen: 2 months, 21 hours
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Re: Jars not colonising :( [Re: AlexP]
#10621580 - 07/04/09 03:48 PM (4 months, 16 days ago) |
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Definitely dunk and roll it is not brutal, far from it. if you wanted more water wouldn't you want some one to give you a drink? Glad you watched the videos though
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B Plus for Trade  if Interested
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hooterjh101
Stranger
Registered: 06/22/09
Posts: 38
Last seen: 2 months, 28 days
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Re: Jars not colonising :( [Re: Leadwing]
#10621676 - 07/04/09 04:21 PM (4 months, 16 days ago) |
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Certainly sounds like what happened to me with air exchange. Eventually i lossened up the tin foil lids a bit an viola! fully colonized in 3 weeks waiting for pins.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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What's better, dunking and rolling, or casing?
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Grab every second given to you and make love to it.
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oTEMPESTo
Just let it ride
Registered: 07/04/09
Posts: 13
Loc: MICHIGAN
Last seen: 4 months, 3 days
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Re: Jars not colonising :( [Re: Leadwing]
#10621745 - 07/04/09 04:43 PM (4 months, 16 days ago) |
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Come on man. look up the info for yourself and decide. I wouldnt say this for everything but with these very beginer question's please see the search bar!
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: oTEMPESTo]
#10621768 - 07/04/09 04:53 PM (4 months, 16 days ago) |
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Quote:
What's better, dunking and rolling, or casing?
You'll have bigger yields when spawning to bulk and applying a casing layer but I'd dunk and roll if I was you. Do the previous for your next grow.
--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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blazeup420
?got issues?
Registered: 04/30/09
Posts: 133
Last seen: 1 month, 7 days
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Re: Jars not colonising :( [Re: AlexP]
#10630066 - 07/06/09 12:58 PM (4 months, 14 days ago) |
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i followed the tek exactly and watched rrs vids and same problem...got new spores and tried the old formula so maybe that will work
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I LOVE MY DRUGS
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