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 proudabu
creeper
 
Registered: 12/05/08
Posts: 435
Loc: Under My Bed
Last seen: 6 hours, 56 minutes
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 Re: Jars not colonising :( [Re: AlexP]
#10610971 - 07/02/09 05:07 PM (4 months, 17 days ago) |
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there should be four holes when making cakes to inject in four different spots and i usually put cloth band aids over mine. dont quote me but what would somebody else recommend covering it with a coffee filter and rubber banding it after your done? just an idea i really never did cakes so its not my expertise
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 AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: proudabu]
#10610991 - 07/02/09 05:10 PM (4 months, 17 days ago) |
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A coffee filter is quite useless for keeping contams out. The verm layer is for keeping contams out.
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: AlexP]
#10611027 - 07/02/09 05:16 PM (4 months, 17 days ago) |
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If you guys want to learn something, this is the link to follow.
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,225
Loc: the rain forests
Last seen: 2 months, 28 days
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Re: Jars not colonising :( [Re: AlexP]
#10611058 - 07/02/09 05:20 PM (4 months, 17 days ago) |
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Quote:
AlexP said:
Quote:
you can turn them upside down and what happens is when you do that the mass of everything comes down and creates a vacuum effect and brings more oxygen inside the jar to finish up.
"Don't flip jars. As prisoner said, it screws up the vermiculite filter. CO2 doesn't need to 'drain' out by flipping the jar upside down. That's a bit of disinformation that once typed refuses to die. The reason is the heat produced by the mycelium causes circulation that takes care of the gas exchange." RR
Bullshit! This is a tried and true method to renew growth to stalled jars! Also it's not for CO2 drainage as you said it simply allows the cake to settle this give a brief exchange in gas. I don't give a shit what someone thinks they know if it works and has worked thousands of times you can't dis it!!! It don't do shit to the verm layer that is pure crap!!!! Unless someone doesn't have a clue and tries to shake the damn jar then they deserve what they get. Myc generates heat regardless if the cake is right side up or upside down and that doesn't always take care of gas exchange it simply pushes out gas from the top of the culture.
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal 
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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DWreck
Killin it
Registered: 03/03/09
Posts: 179
Loc: Maine
Last seen: 15 hours, 36 minutes
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Re: Jars not colonising :( [Re: AlexP]
#10611061 - 07/02/09 05:20 PM (4 months, 17 days ago) |
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yea dont cover it. Just take the foil off and losen the lid to provide g/e. was the one whole in the center? Oh and your left over spores should still be good. Just be sure to flame sterilize. Are you gonna watch the vids?
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 7 days
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Re: Jars not colonising :( [Re: DWreck]
#10611120 - 07/02/09 05:33 PM (4 months, 17 days ago) |
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Yeah I'll watch the vids for next time I grow 
The hole is in the center, I've removed the foil, loosened the lids, and put them back.
I see myc in 2 of them at the bottom (although one of them has some wet bits...) 
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Grab every second given to you and make love to it.
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Psuper
W-W-Wyzzerdd
Registered: 02/12/08
Posts: 2,109
Loc: PNW (where else?)
Last seen: 6 hours, 52 minutes
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Re: Jars not colonising :( [Re: DWreck]
#10611158 - 07/02/09 05:41 PM (4 months, 17 days ago) |
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If your syringe has clumps in it, go ahead and dilute it. You want to be inoculating with fairly "light" syringes anyway. A syringe with clumps of spores can make a handful of syringes.
If you're really low, you just use less inoculant. I inoculated a quart of grain with less than 1/4cc; just colonizes a little slower. ~Pixie~
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<------is looking to find a morel patch this year. Maybe smoke-drying some of them. Only found False morels last year.
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guest1
Mycena
Registered: 05/25/09
Posts: 61
Last seen: 1 day, 13 hours
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Re: Jars not colonising :( [Re: Psuper]
#10611210 - 07/02/09 05:50 PM (4 months, 17 days ago) |
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give it 5 weeks. I have seen spores take 5 weeks to show first signs. People are too anxious and after a few minutes or days after inoculation, they panic and think it is a failure. just sit back, as long as there are no contams, let it go.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: guest1]
#10611237 - 07/02/09 05:54 PM (4 months, 17 days ago) |
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Quote:
give it 5 weeks. I have seen spores take 5 weeks to show first signs.
Huummm? Taking too much space for me! I dump them out if they don't show growth for a week in the right conditions.
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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Lostkeys
Fractal Thought Pattern
Registered: 06/06/09
Posts: 784
Loc: Sirius
Last seen: 6 days, 12 hours
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Re: Jars not colonising :( [Re: guest1]
#10611471 - 07/02/09 06:35 PM (4 months, 17 days ago) |
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Yeah five weeks is a bit excessive considering my jars showed myc growth on the third day...
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"And i think it's gonna be a long long time
Till touch down brings me round again to find
I'm not the man they think i am at home
Oh no no no i'm a rocket man
Rocket man burning out his fuse up here alone"
Everything posted in this forum is fictional, only a fool would take the things written here as fact.
For real time cultivation chat go here and type /join #cultivation
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,225
Loc: the rain forests
Last seen: 2 months, 28 days
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Re: Jars not colonising :( [Re: Lostkeys]
#10611500 - 07/02/09 06:41 PM (4 months, 17 days ago) |
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Longest I've waited was three weeks and that was two weeks of hell! I've got patience but not that much!!!
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Lucid_Euphoria
Dualist
Registered: 03/23/05
Posts: 637
Loc: Flipside
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Re: Jars not colonising :( [Re: Leadwing]
#10611519 - 07/02/09 06:46 PM (4 months, 17 days ago) |
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Quote:
Also will the same syringe be good to use again? Its like 2/3 full
Yes, just be sure to flame sterilize the needle before inoculation.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 7 days
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I flame sterilized the needle when I did it the first time, I heard popping - the sound of spores dying??? :'(
Also the needle turned kinda black so I cleaned it off / cooled it with a Dettol wipe, is that ok?
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Grab every second given to you and make love to it.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 7 days
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Re: Jars not colonising :( [Re: Leadwing]
#10614198 - 07/03/09 06:13 AM (4 months, 16 days ago) |
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Sorry the stickers are in the way lol
 
Not the ideal shape of jar I guess
Hole in the top
My jars 
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Grab every second given to you and make love to it.
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Lostkeys
Fractal Thought Pattern
Registered: 06/06/09
Posts: 784
Loc: Sirius
Last seen: 6 days, 12 hours
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Re: Jars not colonising :( [Re: Leadwing]
#10614558 - 07/03/09 08:11 AM (4 months, 16 days ago) |
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Seriously man, watch RogerRabbit's BRF Tek Videos. You won't be sorry, I promise...
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"And i think it's gonna be a long long time
Till touch down brings me round again to find
I'm not the man they think i am at home
Oh no no no i'm a rocket man
Rocket man burning out his fuse up here alone"
Everything posted in this forum is fictional, only a fool would take the things written here as fact.
For real time cultivation chat go here and type /join #cultivation
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 7 days
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Re: Jars not colonising :( [Re: Lostkeys]
#10614621 - 07/03/09 08:36 AM (4 months, 16 days ago) |
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Watching now
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Grab every second given to you and make love to it.
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JMath
Registered: 06/06/09
Posts: 117
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Re: Jars not colonising :( [Re: Leadwing]
#10614781 - 07/03/09 09:15 AM (4 months, 16 days ago) |
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My syringe made popping noises too when I first inoculated, I think it's just from verm that gets stuck up in the needle and heats up and shoots out.
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It's only after we've lost everything that we're free to do anything.
-Chuck Palahniuk
Trying to catch the impossible dream
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,225
Loc: the rain forests
Last seen: 2 months, 28 days
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Re: Jars not colonising :( [Re: JMath]
#10614934 - 07/03/09 09:50 AM (4 months, 16 days ago) |
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It's the solution (spore water) in the needle most of the time.
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: hyphae]
#10615187 - 07/03/09 10:40 AM (4 months, 16 days ago) |
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When the spore water at room temperature hits the metal flame sterilized syringe it makes popping noises!
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 7 days
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Re: Jars not colonising :( [Re: AlexP]
#10619092 - 07/04/09 03:45 AM (4 months, 16 days ago) |
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Also, I put the syringe back in its plastic bag, in a tube, and in a room temp drawer, that's ok yesh?
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Grab every second given to you and make love to it.
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