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 Leadwing
King in my own Mind
 
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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 Jars not colonising :( 
#10610438 - 07/02/09 03:22 PM (4 months, 18 days ago) |
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I inoculated my jars about a week ago now, and still don't see anything!
I sterilised them, did everything properly, have kept them in a plastic container at the right temp...
Wahhh :'(
What could the reason be?
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Grab every second given to you and make love to it.
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Lostkeys
Fractal Thought Pattern
Registered: 06/06/09
Posts: 784
Loc: Sirius
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Re: Jars not colonising :( [Re: Leadwing]
#10610450 - 07/02/09 03:25 PM (4 months, 18 days ago) |
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More information is required. What type of jars? Are your inoculation holes covered? What temperature are you colonizing at?
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"And i think it's gonna be a long long time
Till touch down brings me round again to find
I'm not the man they think i am at home
Oh no no no i'm a rocket man
Rocket man burning out his fuse up here alone"
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Devii
Curious Examiner
Registered: 05/11/09
Posts: 189
Last seen: 7 days, 1 hour
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Re: Jars not colonising :( [Re: Leadwing]
#10610455 - 07/02/09 03:25 PM (4 months, 18 days ago) |
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Where did you get your spores from? They might just be bunk spores...
But more likely, did you allow your jars to cool for 24 hours after you PC'd them? If you inoculate a hot jar, the temperature will kill your spores.
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Psuper
W-W-Wyzzerdd
Registered: 02/12/08
Posts: 2,110
Loc: PNW (where else?)
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Re: Jars not colonising :( [Re: Leadwing]
#10610462 - 07/02/09 03:26 PM (4 months, 18 days ago) |
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Grains or cakes? What kind of filter? What temps? How many jars? Is their excessive moisture?
You don't need them in a plastic tub. Can you open a jar and smell it? A sour or old sock smell is a sign of bacteria. No signs of growth in 7days means something is wrong. ~Pixie~
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<------is looking to find a morel patch this year. Maybe smoke-drying some of them. Only found False morels last year.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610760 - 07/02/09 04:31 PM (4 months, 18 days ago) |
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4 jars
2 part Verm, 1 part BRF, 1 part water
Around 26C
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Grab every second given to you and make love to it.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610771 - 07/02/09 04:33 PM (4 months, 18 days ago) |
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I covered the jars (each with a hole in) with 2-3 layers of foil, secured it tightly.
I didn't cover up the inoculation holes after I inoculated them, as it had dry Verm ontop.
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Grab every second given to you and make love to it.
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 AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10610812 - 07/02/09 04:40 PM (4 months, 18 days ago) |
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Quote:
I covered the jars (each with a hole in) with 2-3 layers of foil, secured it tightly.
There's no gas exchange that way. Dry verm on top of the inoc holes? Never heard of anything like that...
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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blazeup420
?got issues?
Registered: 04/30/09
Posts: 133
Last seen: 1 month, 7 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610816 - 07/02/09 04:41 PM (4 months, 18 days ago) |
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ive had the same problem twice still cant figure it out...is your BRF organic?mines not but that shouldnt matter...but ya i need to figure this out to...
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 I  LOVE  MY  DRUGS 
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: AlexP]
#10610823 - 07/02/09 04:42 PM (4 months, 18 days ago) |
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No, I mean the jars are 3/4 full of mix, and the last 1/4 is dry Verm.
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Grab every second given to you and make love to it.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610833 - 07/02/09 04:43 PM (4 months, 18 days ago) |
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Also... gas exchange?
Surely they need to be pretty much air tight so theres no chance of contams?
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Grab every second given to you and make love to it.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10610854 - 07/02/09 04:47 PM (4 months, 18 days ago) |
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Quote:
Surely they need to be pretty much air tight so theres no chance of contams?
Air tight jars will stall. Haven't you watched the videos? What are you doing?... What are you following?... Remove that foil and what type of filter?... Who do the lids look like? The verm layer is there to stop contams from attacking your mycelium or substrate.
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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kaneabel
getting my feet wet
Registered: 01/10/05
Posts: 133
Loc: Central Indiana
Last seen: 17 days, 3 hours
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Re: Jars not colonising :( [Re: Leadwing]
#10610860 - 07/02/09 04:48 PM (4 months, 18 days ago) |
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The verm acts as a filter for the air, it should exchange air ok, sometimes near the end of colonization if it seems like it is stalling, you can turn them upside down and what happens is when you do that the mass of everything comes down and creates a vacuum effect and brings more oxygen inside the jar to finish up.
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610888 - 07/02/09 04:53 PM (4 months, 18 days ago) |
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Guide
I must admit, I sort of rushed things...
I still have half a syringe left, might have another go.
Also, is it normal that the syringe had clumps in it? It looked clear apart from the clumps. (I shook it up as much as poss)
The jars I used have screw on tops
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Grab every second given to you and make love to it.
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DWreck
Killin it
Registered: 03/03/09
Posts: 180
Loc: Maine
Last seen: 4 hours, 43 minutes
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Re: Jars not colonising :( [Re: Leadwing]
#10610897 - 07/02/09 04:55 PM (4 months, 18 days ago) |
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well first thing take that foil off..its only used to keep out the water while p/cing. how many holes in your lid? how deep is your dry verm layer? But honestly, It sounds like you need to go watch rr's videos.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: kaneabel]
#10610901 - 07/02/09 04:56 PM (4 months, 18 days ago) |
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Quote:
you can turn them upside down and what happens is when you do that the mass of everything comes down and creates a vacuum effect and brings more oxygen inside the jar to finish up.
"Don't flip jars. As prisoner said, it screws up the vermiculite filter. CO2 doesn't need to 'drain' out by flipping the jar upside down. That's a bit of disinformation that once typed refuses to die. The reason is the heat produced by the mycelium causes circulation that takes care of the gas exchange." RR
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: AlexP]
#10610913 - 07/02/09 04:58 PM (4 months, 18 days ago) |
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--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610916 - 07/02/09 04:58 PM (4 months, 18 days ago) |
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Just the one (tiny) hole... I'll go take the foil off
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Grab every second given to you and make love to it.
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AlexP
It hurts to set you free...
Registered: 06/04/09
Posts: 2,055
Loc: All The Colors
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Re: Jars not colonising :( [Re: Leadwing]
#10610929 - 07/02/09 05:00 PM (4 months, 18 days ago) |
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And loosen up the lids. 
Edit: And watch the videos!!! Watch a professional do the work and then follow!
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"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa
"I think we are blind. Blind people who can see, but do not see." José Saramago
"How can you prove whether at this moment we are sleeping, and all our thoughts are a dream; or whether we are awake, and talking to one another in the waking state?" Plato
"Cowards die many times before their deaths; the valiant never taste of death but once." William Shakespeare
"The foundation of empire is art and science. Remove them or degrade them, and the empire is no more. Empire follows art and not vice versa as Englishmen suppose." William Blake
Edited by AlexP (07/02/09 05:01 PM)
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DWreck
Killin it
Registered: 03/03/09
Posts: 180
Loc: Maine
Last seen: 4 hours, 43 minutes
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Re: Jars not colonising :( [Re: Leadwing]
#10610952 - 07/02/09 05:04 PM (4 months, 18 days ago) |
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There's supposed to be 4 holes. You definitely need to check out the link Alex posted. if you follow that to the t You will succeed
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Leadwing
King in my own Mind
Registered: 08/19/08
Posts: 208
Last seen: 3 months, 8 days
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Re: Jars not colonising :( [Re: Leadwing]
#10610966 - 07/02/09 05:06 PM (4 months, 18 days ago) |
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Oh holy crap wicked, I think there's some signs of life at the bottom of 2 of the jars! WOOOOOT
Also will the same syringe be good to use again? Its like 2/3 full
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Grab every second given to you and make love to it.
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