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 M11
White Thumb
Registered: 06/14/09
Posts: 1,840
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 I have some doubts about my 1st PF Tek attempt... 
#10582377 - 06/27/09 05:20 PM (14 years, 9 months ago) |
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I loaded and sterilized all of my jars late last night, and I inoculated them this morning.
I followed the PF Tek guide exactly, except that I completely forgot to cover the lids with tin foil. I covered the needle holes with professional grade masking tape though. The lids are two parts, but with the masking take covering the needle holes, the two lids stuck together as one. I kept the lids loose to allow steam in. I added foil to cover all of the jars about half way through the sterilization process. I also checked one of the jars to see if it had taken up more water than it should have. The dry verm layer still appeared pretty dry to me. It seemed like it was slightly steamed, but that is expected. Also, I think the upward facing rubber ring may have served as a buffer against water flowing into the jars.
1. Did I really fuck up or should I be ok? The water in the jars appeared like it did before, but it may have been changed.
2. I see that the tek says that when using finer verm, the lids should be a little loose during incubation. Is this indeed correct? I used finer verm, but loosening those jars seems like it is just asking for contaminants. Should I loosen the jars?
Just another note. I was SUPER sterile. I wiped down the room I inoculated in with 91% iso including the floor. I wore a face mask and sterile glove. I flame sterilized the needle. I iso sterilized the containers the jars are incubating in. I iso sterilized the rims of the large pot and lid I used for steaming. I iso sterilized my hands before I put my gloves on.
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 jobot37
Edible hunter
Registered: 09/20/04
Posts: 336
Loc: Oregon, Willamette Valley
Last seen: 2 years, 2 months
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Re: I have some doubts about my 1st PF Tek attempt... *DELETED* [Re: M11]
#10582410 - 06/27/09 05:25 PM (14 years, 9 months ago) |
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Post deleted by jobot37Reason for deletion: HA! j/k
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M11
White Thumb
Registered: 06/14/09
Posts: 1,840
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Re: I have some doubts about my 1st PF Tek attempt... [Re: jobot37]
#10582457 - 06/27/09 05:36 PM (14 years, 9 months ago) |
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I did loosen the jars when I steamed sterilized them. Also, wouldn't the vapor pressure in the pot and in the jar reach equilibrium? Wouldn't their equilibrium be more related to moisture content than heavy condensation? I would think that same heavy condensation, assuming your pot is 100% sealed, would be steamed and vaporized in a similar way. In other words, the heavy condensation would eventually be vaporized again and then enter into the vapor equilibrium anyway.
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M11
White Thumb
Registered: 06/14/09
Posts: 1,840
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Re: I have some doubts about my 1st PF Tek attempt... [Re: M11]
#10582536 - 06/27/09 05:53 PM (14 years, 9 months ago) |
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I am mostly concerned with question 2.
Should the jars be loose or tight? I would think that loose jars would allow entry of contaminants. What should I do?
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backintheriver
Registered: 08/21/08
Posts: 634
Last seen: 1 year, 8 months
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Re: I have some doubts about my 1st PF Tek attempt... [Re: M11]
#10582567 - 06/27/09 06:01 PM (14 years, 9 months ago) |
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the dry verm layer is ment to stop contams. some people take the tape off after pcing.
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M11
White Thumb
Registered: 06/14/09
Posts: 1,840
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Re: I have some doubts about my 1st PF Tek attempt... [Re: backintheriver]
#10582641 - 06/27/09 06:20 PM (14 years, 9 months ago) |
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So I should loosen them a little and you think that I am fine as far as contams go even though I didn't use the foil?
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK 
Last seen: 4 years, 3 months
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Re: I have some doubts about my 1st PF Tek attempt... [Re: M11]
#10582772 - 06/27/09 06:43 PM (14 years, 9 months ago) |
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The jar lids are tight during sterilisation and incubation. You don't need to worry about CLEANING AN INCUBATOR WITH ALCOHOL. 91% isn't as effective as 70% its not a steriliser only a sanitiser.
inoc through the tape annd leave uncovered.
If your ambient temps are around 75f don't bother with incubation
--------------------
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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M11
White Thumb
Registered: 06/14/09
Posts: 1,840
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Re: I have some doubts about my 1st PF Tek attempt... [Re: veda_sticks]
#10582904 - 06/27/09 07:09 PM (14 years, 9 months ago) |
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Thanks.
So if I replaced the tape on them (the tek guide said to) and the jar lids are currently tight, I should loosen the jars a bit? I know you said they should be tight during incubation, but if you don't cover the needle holes and I did, should I loosen the jar lids a tad?
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 3 months
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Re: I have some doubts about my 1st PF Tek attempt... [Re: M11]
#10582962 - 06/27/09 07:20 PM (14 years, 9 months ago) |
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Leave the jar lids tight and leave the inoc holes uncovered it its micropore tape you can leave it on it breathes.
www.mushroomvideos.com
And wartch the pf tek videos there
--------------------
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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M11
White Thumb
Registered: 06/14/09
Posts: 1,840
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Re: I have some doubts about my 1st PF Tek attempt... [Re: veda_sticks]
#10582994 - 06/27/09 07:26 PM (14 years, 9 months ago) |
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Thanks a lot. That is exactly what I needed. I am amazed by the quick, concise and effective answers by the members on this site. It is wonderful.
Do you think I will be ok although I didn't use tin foil?
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cyb3rtr0n
searching for truth
Registered: 05/06/09
Posts: 1,832
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Re: I have some doubts about my 1st PF Tek attempt... [Re: M11]
#10583336 - 06/27/09 08:25 PM (14 years, 9 months ago) |
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the tin foil is mainly to prevent the substrate in the jars from getting soaked by the water(condensation) dripping from above in the pressure cooker.
So as long as your jars didnt get waterlogged, I would think you should be ok. Also, if you used the layer of verm on the top of the cakes, that may have absorbed a little moisture leaking in.
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