Just to clarify... - Gourmet and Medicinal Mushrooms

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Shop for: Paul Stamets, Petri Dish

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Mephistophelian
Quasi Hob-Nobbery

Registered: 08/14/08
Posts: 2,509
Loc: Camp Crystal Lake

Just to clarify...

#10220736 - 04/23/09 05:20 PM (4 years, 29 days ago)

When I go to prepare my slants. I want the leading edge...now, if in the case of a petri dish that has grown out to the edges of the dish as opposed to half-way, does that count as the leading edge from the original inoculation or do you drop a new piece into a new dish and go with that edge (which seems counter-productive to me).

E- I have it answered.

Edited by Mephistophelian (04/23/09 08:03 PM)

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flugelizor
Furious ball of nothing

Registered: 11/16/08
Posts: 405
Loc: Western NY
Last seen: 1 day, 3 hours

Re: Just to clarify...

[Re: Mephistophelian]
#10220801 - 04/23/09 05:57 PM (4 years, 29 days ago)

I'm kind of a noob - but I have seen lots of petris contaminate - so here are my thoughts:

The reason to get it far from the center - you are hoping the myc. will outrun any contamination on the original sample.

Also try to get it before it reaches the edge; because any contamination that seeps in under the lid usually happens around the edge. This is something I read in a Stamets book and it makes sense.

If it has reached the edge and you want to give it a try anyway - I would take a sample from halfway in.
Furthest from any contams in the center, and the edge.

Good luck.

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Mephistophelian
Quasi Hob-Nobbery

Registered: 08/14/08
Posts: 2,509
Loc: Camp Crystal Lake

Re: Just to clarify...

[Re: flugelizor]
#10221471 - 04/23/09 07:52 PM (4 years, 29 days ago)

--------------------
Exotic-Culture Co-Op Thread, Come Have A Look!

Medicinal Notations on Mushrooms

Master Slants Made Simple

Edited by Mephistophelian (04/23/09 08:02 PM)

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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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