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 BaKeRx561x420
Pound 'em till you piss
Registered: 09/28/07
Posts: 368
Loc: Nowhere, Kansas
Last seen: 1 year, 2 months
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 hot weather... bad for shipping cultures? 
#10180994 - 04/16/09 11:22 PM (4 years, 2 months ago) |
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I order some culture syringes and when they arrived they sat in my aluminum mailbox for 1-2 hours right after 10 o'clock (to give you an idea of the intensity of the sun). I went inside and checked the weather channel( ) and it said the temp for my area was 83°(S FLA), but it was obviously hotter in the mailbox. The syringes felt kinda warm too the touch.
Basically, what I'm trying to get at is are my cultures a crap shot now?
If they are, I'm not sure if I should contact the vendor, because I kinda feel like it's totally my fault.
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Get some experience under your belt or shut the fuck up.
RR
FUCK YOU TRICHODERMA
  
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 Roger Fudd
masshuru-mu uekiya
Registered: 01/27/09
Posts: 420
Loc: Earth, Inner Solar System
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Re: hot weather... bad for shipping cultures? [Re: BaKeRx561x420]
#10181203 - 04/16/09 11:56 PM (4 years, 2 months ago) |
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Put one drop onto agar in a petri dish and see if it grows. That's pretty much the only way to tell if it's viable or not. What strain was the culture? Some strains are more heat tolerant than others, but ~90 F is pretty hot...
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BaKeRx561x420
Pound 'em till you piss
Registered: 09/28/07
Posts: 368
Loc: Nowhere, Kansas
Last seen: 1 year, 2 months
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Re: hot weather... bad for shipping cultures? [Re: Roger Fudd]
#10181319 - 04/17/09 12:20 AM (4 years, 2 months ago) |
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Quote:
Roger Fudd said:
What strain was the culture?
Pleurotus citrinopileatus, Golder Oyster
and
Hericium erinaceus, Lion's Mane Mushroom
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Get some experience under your belt or shut the fuck up.
RR
FUCK YOU TRICHODERMA
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OZZ
K.I.S.S.
Registered: 06/08/02
Posts: 919
Last seen: 1 month, 9 days
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Re: hot weather... bad for shipping cultures? [Re: BaKeRx561x420]
#10181588 - 04/17/09 01:04 AM (4 years, 2 months ago) |
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Im sure your fine, try the syringes first before you contact the vendor, no point in contacting them if the syringes are fine.
Put some to agar or shoot up a grain jar and give it a week or two. No hurry with these things.
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lethargic
Stranger
Registered: 03/09/09
Posts: 351
Loc: my humble abode
Last seen: 2 years, 9 days
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Re: hot weather... bad for shipping cultures? [Re: BaKeRx561x420]
#10181593 - 04/17/09 01:05 AM (4 years, 2 months ago) |
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Hope they are alive and kicking.. I guess the best bet is to order next day delivery on those type of things?
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PitcherCrab
Crescent Fresh
Registered: 12/02/06
Posts: 1,212
Loc: The bottom of the sea.
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Re: hot weather... bad for shipping cultures? [Re: lethargic]
#10183431 - 04/17/09 10:52 AM (4 years, 2 months ago) |
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You're all good. Mycelium is very resilient. If it doesn't work contact the vendor (sporeworks?) and they will send you a new culture. I got in touch with them after 8 months after my initial order and they had a fresh culture to me within 3 days.
RR said: "Thermal death does not occur at 106F, despite published reports otherwise. I routinely pour 130F hot agar over established mycelium in petri dishes, and have never killed the mycelium yet. Perhaps an extended time at high temperatures would be fatal, but not short periods."
(From this post: http://www.shroomery.org/forums/showflat.php/Number/7724981#7724981)
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"When they tell you to grow up, they mean stop growing." - Tom Robbins
"Art should not whore itself to the marketplace, nor should it whore itself to the interior decoration industry. Art should set the agenda." - Terrance McKenna
My GH & Humidibucket Setup Blue Oyster Bucket Grow Pink Oyster Laundry Basket Pioppino Shotgun Grow EvilMushroom666's Wedge Tek
Edited by PitcherCrab (04/17/09 10:53 AM)
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jjb007
Registered: 10/03/08
Posts: 338
Loc: Near Philly
Last seen: 3 hours, 55 minutes
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Re: hot weather... bad for shipping cultures? [Re: PitcherCrab]
#10183677 - 04/17/09 12:01 PM (4 years, 2 months ago) |
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Quote:
PitcherCrab said:
You're all good. Mycelium is very resilient. If it doesn't work contact the vendor (sporeworks?) and they will send you a new culture. I got in touch with them after 8 months after my initial order and they had a fresh culture to me within 3 days.
RR said: "Thermal death does not occur at 106F, despite published reports otherwise. I routinely pour 130F hot agar over established mycelium in petri dishes, and have never killed the mycelium yet. Perhaps an extended time at high temperatures would be fatal, but not short periods."
(From this post: http://www.shroomery.org/forums/showflat.php/Number/7724981#7724981)
I am just curious why RR would pour agar over an established mycelium in a petri dish?
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BaKeRx561x420
Pound 'em till you piss
Registered: 09/28/07
Posts: 368
Loc: Nowhere, Kansas
Last seen: 1 year, 2 months
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Re: hot weather... bad for shipping cultures? [Re: jjb007]
#10184417 - 04/17/09 02:35 PM (4 years, 2 months ago) |
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Gawd, I feel like an idiot now. I swear I've read about the pouring agar on mycelium at least five different times in posts from RR. I don't know why I didn't recall that; I guess the minor panic attack clouded my brain.
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Get some experience under your belt or shut the fuck up.
RR
FUCK YOU TRICHODERMA
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PitcherCrab
Crescent Fresh
Registered: 12/02/06
Posts: 1,212
Loc: The bottom of the sea.
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Re: hot weather... bad for shipping cultures? [Re: jjb007]
#10184967 - 04/17/09 04:07 PM (4 years, 2 months ago) |
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Quote:
jjb007 said:I am just curious why RR would pour agar over an established mycelium in a petri dish?
I started a thread about 2 days ago where we were talking all about this... its called something like "isolating vs. cleaning a print". You do it because the contams can't get through the new layer of agar but the mycelium can so its used as a way to get a clean culture from dirty spore or a dirty culture.
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"When they tell you to grow up, they mean stop growing." - Tom Robbins
"Art should not whore itself to the marketplace, nor should it whore itself to the interior decoration industry. Art should set the agenda." - Terrance McKenna
My GH & Humidibucket Setup Blue Oyster Bucket Grow Pink Oyster Laundry Basket Pioppino Shotgun Grow EvilMushroom666's Wedge Tek
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lethargic
Stranger
Registered: 03/09/09
Posts: 351
Loc: my humble abode
Last seen: 2 years, 9 days
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Re: hot weather... bad for shipping cultures? [Re: PitcherCrab]
#10189082 - 04/18/09 12:31 PM (4 years, 2 months ago) |
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Man, mycelium is a tough fucker.
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