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 Mycelio
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Registered: 06/24/08
Posts: 1,636
Loc: Berlin 
Last seen: 4 months, 12 days
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 Agaricus bisporus on non composted substrate 
#10002498 - 03/19/09 06:39 PM (4 years, 2 months ago) |
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Hello,
just got asked to describe my way of growing A. bisporus without composting large amounts of manure, so here it is. It is simple and works quite well, though the yield is lower than on professional mushroom compost.
The Substrate:
I use a mixture of equal amounts of straw and horse manure pellets. Who can't get hold of horse manure, should replace it by cow manure, worm castings or garden compost. As preparation, I fill the dried manure in a pot, add water until it is slightly covered, let it soak and cook it with low heat for a few minutes. Attention, it foams and boils over, so you better stay with it, than cleaning the stove from manure slurry! Then I add the straw pellets and mix. If the water content isn't correct, I add more pellets or a small amount of water. Then I close the lid, cover with a towel and let it cool slowly. Afterwards I mix in some gypsum or lime (ground egg shells).
The source material:
To get started, I filled some tiny jars with substrate and sterilized them. Adding soaked grain is an option, but raises th possibility of contams. For inoculation I used mycelium on agar as well as pieces of gill tissue from supermarket mushrooms. Of course the gill tissue died, but stayed alive for long enough to cause spore germination after ten days. Spores alone would have slept for a month. After full colonization, I let the jars sit around for a few weeks.
Multiplying Spawn:
Ca. every three weeks, I break up the colonized substrate carefully with a teaspoon, fill it into bigger jars and mix in an equal amount of freshly cooked substrate. By breaking into small pieces and mixing well, there are many many inoculation points and the mycelium only has to grow a few millimeters to colonize its new food. This gets repeated until I have enough substrate. In the final step I fill it into a clean flower pot and keep it under high humidity, like in a plastic box, where I spray water at the side walls and under the lid. I do all the mixing open air on the kitchen table and didn't have contams so far.
Casing:
Another two or three weeks later I mix peat with some gypsum and ground egg shells, moisten it and cover the substrate in the flower pot with a few centimeters (ca. one inch). Sometimes I cover with used potting soil or I drop some rye grass seeds on top. I have never heated or applied chemicals to those materials. After a few days the mycelium races through the casing layer. As soon as the mycelium appears at the surface I move the pot into a small greenhouse with low humidity (no misting, no ultrasonic humidifier) and more fresh air. Some days later primordia formation starts and within one or two weeks mushrooms develop.
Addition: In between I tried casing with garden soil and ran into problems with mites, worms and mold. Here I recommend pasteurization.
Caveats:
Substrate and casing layer should be moist, but not wet. If there is water logging in all the pores, it blocks the mycelium from growth and from breathing. When the casing layer dries out, just spray a little water, but don't spray on the developing mushrooms to avoid problems with bacteria, which cause brown spots and rotting.
After two flushes the substrate seems to be worn out. Then it's time to bury it in the garden or flower pot. Sometimes the mycelium survives and keeps on fruiting.
A few images, first the brown strain from agar:
   
mycelium on agar and spawn jars
  
mixed substrate after six days and primordia.
  
harvest
Then the white strain from supermarket spores:
  
spawn, mixed substrate and harvest
in a flower pot
Carsten
Edited by Mycelio (10/10/09 05:13 PM)
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falcon
In the green
Registered: 04/01/02
Posts: 5,832
Last seen: 17 hours, 49 minutes
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Re: Agaricus bisporus on non composted substrate [Re: Mycelio]
#10002731 - 03/19/09 07:24 PM (4 years, 2 months ago) |
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Very cool!
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Arkady
Stranger
Registered: 02/20/09
Posts: 330
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Re: Agaricus bisporus on non composted substrate [Re: falcon]
#10005635 - 03/20/09 03:03 AM (4 years, 2 months ago) |
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How did they taste?
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 4 months, 12 days
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Re: Agaricus bisporus on non composted substrate [Re: falcon]
#10006243 - 03/20/09 08:21 AM (4 years, 2 months ago) |
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Thanks, though the yield isn't overwhelming. Must be because the straw is not partially digested by microbes... I'll try cold composting of that mixture in the worm bin now.
Carsten
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 4 months, 12 days
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Re: Agaricus bisporus on non composted substrate [Re: Arkady]
#10006247 - 03/20/09 08:23 AM (4 years, 2 months ago) |
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They were delicious, tasted stronger than storebought ones.
Carsten
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 Blutjager
Inhuman
Registered: 06/12/06
Posts: 9,220
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Re: Agaricus bisporus on non composted substrate [Re: Mycelio]
#10006523 - 03/20/09 10:20 AM (4 years, 2 months ago) |
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Who sells a culture to grow these,I admit I have not looked because I'm sure at this point I would screw it up anyway but am just throwing it out in case some one has a book mark handy that I can put away for future reading.I really like the looks of this grow.That 1st pic in the third row is beautiful
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Cake makers please read this before you waste more time/effort >>
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6236058/an/0/page/0
My LC tek >> http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7320516/an/0/page/0
""THEY CAN NOT KILL ME,I STILL WALK THE EARTH,WEAKENED BUT ALIVE.THEY HAVE TAKEN ALL FROM ME BUT THE BEATING OF MY COLD DEAD HEART.
THEY WILL NOW LEARN OF FEAR,THESE MEWING BABIES WHO THOUGHT THEY COULD DESTROY ME.
I HAVE REGAINED MY POWERS AND SHALL COME TO THEM IN THE NIGHT........
MY VISITS SHALL NOT BE KIND !! ""
 BlutJager
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Paresthesia
Stranger
Registered: 07/02/08
Posts: 1,051
Loc: Texas
Last seen: 1 year, 3 months
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Re: Agaricus bisporus on non composted substrate [Re: Blutjager]
#10007084 - 03/20/09 12:50 PM (4 years, 2 months ago) |
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Sporeworks sells an Agaricus bitorquis culture, and I've gotten Agaricus subrufescens grain spawn from a company up in Wisconsin. Also, wild Agaricus campestris mushrooms are pretty easy to find, but they tend to have a short shelf life. You also need to catch them just as they're coming up, because insects start laying eggs in them while they're still buttons.
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"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."
- T. S. Eliot
I'm currently looking for cultures of the following species:
Calocybe indica, Chlorophyllum rachodes, Lentinula boryana, Polyporus umbellatus
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solumvita
Q.B.E.
Registered: 02/12/08
Posts: 1,975
Loc: South Africa
Last seen: 19 days, 9 hours
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Re: Agaricus bisporus on non composted substrate [Re: Paresthesia]
#10007811 - 03/20/09 03:07 PM (4 years, 2 months ago) |
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Good grow, thanks for the pics
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One of these days all the answers will be revealed until then we learn from each other!
www.mushrush.co.za
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SuchSmartMonkeys
mycologically driven individual
Registered: 10/26/05
Posts: 1,055
Last seen: 1 month, 18 days
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Re: Agaricus bisporus on non composted substrate [Re: solumvita]
#10009004 - 03/20/09 06:26 PM (4 years, 2 months ago) |
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why does no one cultivate Agaricus augustus? If this were possible, i would be all over that shit... Probably the best mushroom i've ever tasted...
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falcon
In the green
Registered: 04/01/02
Posts: 5,832
Last seen: 17 hours, 49 minutes
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Re: Agaricus bisporus on non composted substrate [Re: SuchSmartMonkeys]
#10009172 - 03/20/09 07:01 PM (4 years, 2 months ago) |
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Agaricus blazei and A. subrufescens both taste like A. augustus.
Both species' culture are for sale. The growth parameters are
very close to A. bisporus, but the yeilds aren't as good.
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solumvita
Q.B.E.
Registered: 02/12/08
Posts: 1,975
Loc: South Africa
Last seen: 19 days, 9 hours
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Re: Agaricus bisporus on non composted substrate [Re: SuchSmartMonkeys]
#10011506 - 03/21/09 02:28 AM (4 years, 2 months ago) |
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No one has tried hard enough?
Maybe you should try and see, most likely you would have to generate your own cultures etc
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One of these days all the answers will be revealed until then we learn from each other!
www.mushrush.co.za
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Paresthesia
Stranger
Registered: 07/02/08
Posts: 1,051
Loc: Texas
Last seen: 1 year, 3 months
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Re: Agaricus bisporus on non composted substrate [Re: solumvita]
#10012430 - 03/21/09 09:44 AM (4 years, 2 months ago) |
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There are a few threads here on A. augustus. One from 2001 mentions that they require disturbed habitats to stimulate fruiting. I've never seen or eaten one, so I have no idea. Fungi Perfecti sells a culture. ($125!)
I just checked my A. subrufescens spawn, and it looks like it has trich growing on it! I don't know, though. Doesn't agaricus mycelium look like mold?
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"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."
- T. S. Eliot
I'm currently looking for cultures of the following species:
Calocybe indica, Chlorophyllum rachodes, Lentinula boryana, Polyporus umbellatus
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 4 months, 12 days
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Re: Agaricus bisporus on non composted substrate [Re: Paresthesia]
#10012597 - 03/21/09 10:45 AM (4 years, 2 months ago) |
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I wouldn't say Agaricus mycelium looks like mold, but that depends on the type of mold you have in mind.
Trichoderma can be a problem, if your culture is not healthy and strong, especially on experimental substrate formulas. See here for some background information on the interaction of trich and agaricus and for nice microscope images:
http://www.ijat-rmutto.com/pdf/JUl_V2_06/3_mohammadi.pdf
Carsten
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