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 Kiada09
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Registered: 02/24/09
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 Best Glovebox Method? 
#9868120 - 02/26/09 06:47 AM (3 years, 2 months ago) |
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On the RR videos he just has holes in his box, and uses disposable gloves and a sleeve - whereas in most of the others Ive looked at they have gloves *attached* to the holes on the inside? Which is best / cleanest? Making my glovebox as we speak 
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iskinbash
Registered: 09/25/08
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Re: Best Glovebox Method? [Re: Kiada09]
#9868131 - 02/26/09 06:50 AM (3 years, 2 months ago) |
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You could do it either way. the whole point is to not allow air flow in or out of the GB after you sanitize it, so i suspect attaching gloves might be a little more worthwhile.
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*iskinbash is merely a fictional character placed in a real life environment.
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Muffinlad
the hippie
Registered: 12/18/08
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Re: Best Glovebox Method? [Re: iskinbash]
#9868161 - 02/26/09 07:03 AM (3 years, 2 months ago) |
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I made a glove box, like the one directed in the video. I found it complicated to use. I decided to clean the closet walls with Lysol and washed the walls down, closet shelf, and everything near my space I was using as a table. Elevated maybe 3-4ft. off the ground I had a sterlite tub on my rack and used its top surface as my table. I washed my hands, top surface, and the outside of my jars with alcohol. Then shot up all my jars on my "table" with no gloves on, still no contamination. If you find that using a glove box is easy than by all means go for it, but this is just how I did it. Alot less of a pain.
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 PitcherCrab
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Re: Best Glovebox Method? [Re: Muffinlad]
#9868405 - 02/26/09 08:26 AM (3 years, 2 months ago) |
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IMO integrated gloves are a pain in the ass, although tyvek sleeves might be worth it. Just make a glove box and use it without the gloves. Put in flexible plastic air ducting in the arm holes to help create a better seal around your forearms. Before use wipe it out with some 70% alcohol, then put in what your working with along with some paper towel soaked in alcohol, wait 5-10min, "wash" your hands and arms with alcohol and your good to go. I do all of my sterile work in a box with this exact sterile procedure and I rarely if ever get airborne contams. Get the biggest tub you can, you will be glad you did. I use a 106qt sterilite locking lid tub with a piece of plexiglas hot glued into the top.
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Kiada09
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Re: Best Glovebox Method? [Re: PitcherCrab]
#9868424 - 02/26/09 08:35 AM (3 years, 2 months ago) |
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This is harder than it looks.. I think I made the holes a bit big cause I didn't think I could fit my hands comfortably through a CD size hole (bassplayers hands! haha).. and just basically ballsed up making the sleeve.. but it can't have been the right material on the envelope. Not sure we even have specific 'Tyvek' envelopes here in the UK.. but ehh I'll keep trying. Probably need a bigger box though.. mines 32 litre.
'Put in flexible plastic air ducting in the arm holes'
What kind of material / could you link a picture to something like this so I can see what you mean please?  thanks lots.
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ICdeadPeople
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Re: Best Glovebox Method? [Re: Muffinlad]
#9868476 - 02/26/09 08:51 AM (3 years, 2 months ago) |
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Quote:
Muffinlad said:
I made a glove box, like the one directed in the video. I found it complicated to use. I decided to clean the closet walls with Lysol and washed the walls down, closet shelf, and everything near my space I was using as a table. Elevated maybe 3-4ft. off the ground I had a sterlite tub on my rack and used its top surface as my table. I washed my hands, top surface, and the outside of my jars with alcohol. Then shot up all my jars on my "table" with no gloves on, still no contamination. If you find that using a glove box is easy than by all means go for it, but this is just how I did it. Alot less of a pain.
glovebox too complicated? Seems pretty simple to me, u put ure 2 hands in 2 holes and work like u normally would  WTF is complicated? Ir seems like cleanig a closet out would be more complicated and more work.
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13shrooms
Lightning Shaman
Registered: 01/01/09
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if you attach any type of glove to your box it makes it a bit more difficult to clean.
I just melted (with coffee can) two holes and tah dah. nice still air box.
but if your work area is draft free and very clean you dont need one, its just good practice.
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iskinbash
Registered: 09/25/08
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Re: Best Glovebox Method? [Re: 13shrooms]
#9868568 - 02/26/09 09:15 AM (3 years, 2 months ago) |
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I never use a glovebox for inoculations and my grows have gone well...but now that i got agar and petri dishes, i went ahead and made one.
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*iskinbash is merely a fictional character placed in a real life environment.
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Doodle
fuck off
Registered: 06/08/08
Posts: 1,069
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Re: Best Glovebox Method? [Re: 13shrooms]
#9868602 - 02/26/09 09:23 AM (3 years, 2 months ago) |
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heres the one i use, and have used similar still air boxes for every grow and havent had a contam since my very first grow back in 03 except for trich which came from casing procedures done in open air.
Hell i even did a batch of jars open air while drunk the other day and no contams. I dont advise it but as long as you use sterile procedures.
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13shrooms
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Re: Best Glovebox Method? [Re: Doodle]
#9868629 - 02/26/09 09:30 AM (3 years, 2 months ago) |
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I turn mine upside down cuz my lid is not clear, that way I can see everything and dont have to look through one area.
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Doodle
fuck off
Registered: 06/08/08
Posts: 1,069
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Re: Best Glovebox Method? [Re: 13shrooms]
#9868641 - 02/26/09 09:33 AM (3 years, 2 months ago) |
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Shit I'm so stupid i never even thought of that, Great idea. it does get hard seeing the tops of the jars with knocking em up with a solid lid.
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shroomer17
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Re: Best Glovebox Method? [Re: Doodle]
#9868653 - 02/26/09 09:35 AM (3 years, 2 months ago) |
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I attached my gloves to my glove box and sealed them to make it air tight. Then I started getting a lot more contams. I detached the gloves and now use disposable latex gloves each time. I think sealing the gloves onto the box was creating pressure in the box when I moved my arms around, and air would get drawn in around the edges of the lid... Anyway, although it works for many people, I would advise against attached gloves and go disposable
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Kiada09
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Re: Best Glovebox Method? [Re: shroomer17]
#9868807 - 02/26/09 10:03 AM (3 years, 2 months ago) |
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Thanks loads for the replies - really appreciate it. I think I will just go without attached gloves and just make sure sterile procedures are 110% followed properly.
Hmmm so, with regards to preparing the jars.. you have the substrate, then the casing layer, then the lid with the 4 holes - and on top of that theres 2 layers of foil with no holes? theres no foil between the casing layer and the lid - just over the top of the lid? Then the foils removed to inoculate, then quickly replaced to incubate / let the jars colonize?
Also (reading the Psilocybin Mushroom Handbook) its best not to use perlite, and fruit right out of the jar? I understand this gives more shapely results, but is the yield as good? And there wouldnt be any need for perlite - but is this method more likely to go wrong?
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13shrooms
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Re: Best Glovebox Method? [Re: Kiada09]
#9868897 - 02/26/09 10:18 AM (3 years, 2 months ago) |
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Quote:
you have the substrate, then the casing layer, then the lid with the 4 holes - and on top of that theres 2 layers of foil with no holes? theres no foil between the casing layer and the lid - just over the top of the lid? Then the foils removed to inoculate, then quickly replaced to incubate / let the jars colonize?
NO, kinda. its not a casing layer its a filter for contams. foil is removed after PCing/steaming and never put back on. its to keep water out and not mess with your moisture content.
www.mushroomvideos.com watch the PF-tek it 4 prts. perfect every time.
I bought it. its got alot of info for diff stuff also.
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Muffinlad
the hippie
Registered: 12/18/08
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Last seen: 3 months, 17 days
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Quote:
ICdeadPeople said:
glovebox too complicated? Seems pretty simple to me, u put ure 2 hands in 2 holes and work like u normally would WTF is complicated? Ir seems like cleanig a closet out would be more complicated and more work.
Try lighting a lighter with gloves on. Pulling your syringe out of the arm hole each and everytime. Lighting it over and over. If you have a half empty closet it's simple. Not to mention less money used in buying a box with the materials needed. Just turn your closet into a sterile enviroment, off the ground that is. Most money spent on that procedure would just be on the disinfectant.
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13shrooms
Lightning Shaman
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Re: Best Glovebox Method? [Re: Muffinlad]
#9869149 - 02/26/09 11:00 AM (3 years, 2 months ago) |
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Quote:
Try lighting a lighter with gloves on. Pulling your syringe out of the arm hole each and everytime. Lighting it over and over. If you have a half empty closet it's simple. Not to mention less money used in buying a box with the materials needed.
1st, you dont use a lighter. you use a alcohol lamp(clean flame) lighters use butane and leaves a residue on your needle(not exactly clean).
you can make a lamp with a shotglass, a piece of foil and a cotton ball. fill shotglass with isoalcohol, cover with foil, put cottonball(shaped into a wick) into a center hole in foil. light once w/lighter and then put your gloves on. (leave it outside of GB.)
2nd, glove box cost about $10.00 + cleaning supplies you are getting anyway.
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Kiada09
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Re: Best Glovebox Method? [Re: 13shrooms]
#9869185 - 02/26/09 11:07 AM (3 years, 2 months ago) |
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gargh just put a crack in my shotgun terrarium by pressing down on the drill too hard. Ahh well, have to buy a new un tomoz.. money! Think I had the holes too close together anyways.. and dont think it was big enough. Should get it right tomoz - wish I had a power drill, this portable takes ages to make a hole haha.
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iskinbash
Registered: 09/25/08
Posts: 1,063
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Re: Best Glovebox Method? [Re: 13shrooms]
#9869188 - 02/26/09 11:07 AM (3 years, 2 months ago) |
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Quote:
13shrooms said:
2nd, glove box cost about $10.00 + cleaning supplies you are getting anyway.
and you dont have to scrub down a closet every time you want to inoculate.
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*iskinbash is merely a fictional character placed in a real life environment.
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Modizu
I'm all M'd outtttt
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Re: Best Glovebox Method? [Re: 13shrooms]
#9869193 - 02/26/09 11:08 AM (3 years, 2 months ago) |
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yeah, definitely make sure you take time and make your glovebox correctly.
I believe an unsanitary glovebox is what botched my 2nd batch of jars.
I thought i had everything clean as shit, but i guess not.
oh and
Quote:
Klada09 said:
Hmmm so, with regards to preparing the jars.. you have the substrate, then the casing layer, then the lid with the 4 holes - and on top of that theres 2 layers of foil with no holes? theres no foil between the casing layer and the lid - just over the top of the lid? Then the foils removed to inoculate, then quickly replaced to incubate / let the jars colonize?
another reason i think my first batch stalled, i had 4 holes and on each hole was micropore tape, and then i had tin foil over that.
I think that was my mistake, if you have micropore tape, dont put tin foil on that as well, i'm pretty sure it makes for poor gas exchange and stalls the jars.
Either use just tinfoil or micropore tape.
Correct me if i'm wrong guys.
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MODiZu
"Don't say no to pills, Ativan™ won't kill!
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13shrooms
Lightning Shaman
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Re: Best Glovebox Method? [Re: iskinbash]
#9869201 - 02/26/09 11:09 AM (3 years, 2 months ago) |
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while your out get a drill bit made to cut plastic, the ones that come with a drill are for wood. it makes a big difference. trust me, I learned the hard way. 3 tubs broke.
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