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 Phallus
I'd fruit that.
Registered: 07/22/08
Posts: 406
Loc: sunshine shit-land
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 Glove Box and Other Questions 
#9795195 - 02/14/09 01:34 PM (4 years, 3 months ago) |
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I had a few questions about a glove box I intend to be making, according to other posts I've read, you fellows would call it a "still air" glove box. I used the design in Psilocybin Mushroom Handbook: Easy Indoor & Outdoor Cultivation, I just had a few questions and possible upgrades.
If I were to construct this out of steel instead of lining cardboard with contact paper, would it be easier to keep sterile? I ask this because I can easily have one fabricated, possibly cheaper than a 2'x 2'x 2' cardboard box.
I haven't decided on what I would be using for the clear covering yet, but how hot is it likely to get inside with an alcohol lamp burning? Do I need to worry about any melting?
I was also considering having rubber placed over the arm holes instead of flaps, and cut them into slices, so as to have numerous triangles covering the holes. This would reduce the amount of unsterilized air entering the environment. Is this worth doing, or is air coming through the arm holes not an issue?
I also have some other, general, questions about cultivating:
Could numerous colonized jars be placed into a tray and fruited, skipping the grain bag inoculation step? Once placed in the tray would the different colonies compete for nutrition, etc?
Can mycelium in the brown rice flour of a PF Tek be used to colonize a grain jar?
Is there a benefit to removing the cakes in a PF Tek and placing them in perlite instead of leaving them in the jars and blocking sunlight to the sides of the jar, inspiring fruiting? It appears to produce more fruits when the cakes are placed in perlite, but does it average out through flushes or is it more/less concentrated over the area available? I'll probably try both methods regardless, just curious.
How big of an issue is senescence, if I were to clone a cubensis from a PF Tek, colonize a grain jar, fruiting in a tray and then cloning one from the tray and repeating said steps would it cause a noticeable decline in quality/efficiency/productivity? If I used spores instead of cloning?
I'm really looking forward to starting, just waiting for spring to come into play so I can go pick some beauties to get spores from.
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fltdriver82
Bow to your Mushroom God
Registered: 01/24/09
Posts: 1,271
Loc: On a trip
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Re: Glove Box and Other Questions [Re: Phallus]
#9795233 - 02/14/09 01:41 PM (4 years, 3 months ago) |
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http://www.shroomery.org/9457/Ghetto-Chamber-Tek
I used this method and line the top (were the top seals with the storage container) with that window door insulation that comes on a roll with one side being sticky. Sorry but i can't for the life of me think what that shits called.
Didn't use sewer rings ($7 each) just used 3" pvc pipe cut to about 6 inches ($1.50) and sealed with painters caulk and construction adhesive on both side.
------------------------------------------------------------------
Sorry didn't read all of your ?'s
For birthing: yes also dunk and roll. The adv. to birthing is that instead of having one side to grow on the mushies can grow on top and all around the sides of the cake=more mushies.
Place on alum. foil on perlite not in it.
Don't know first hand about cloning and re-cloning so I won't even give my opinion.
Don't wait for summer man, splurge the $20 to get a couple syringes and start now, plus you will know exactly what strain you have.
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The Fourth Amendment gives every American the right to refuse any search without a warrant.
Say hello to my friend, his name is George.
Every other ? you have is answered here
[quote]Jimmytoke said:
in soviet russia, mushroom trips on you[/quote]
Edited by fltdriver82 (02/14/09 01:46 PM)
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 CptnGarden
fuck this site
Registered: 05/13/04
Posts: 11,945
Last seen: 4 years, 29 days
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Re: Glove Box and Other Questions [Re: Phallus]
#9795306 - 02/14/09 01:53 PM (4 years, 3 months ago) |
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keep the alcohol lamp out of the glovebox, theres no need for it to be in there.
if you have a lamp burning in there, the air wont be still, it will be moving.
the point is to let all the air inside grow still before you do your work, so everything flying around in there gets a chance to land.
ive used a cardboard box with syran wrap for many innoculations.
just spray the inside with something toxic like lysol, wipe all ur stuff down with isopropyl, place ur work inside and close up ur box, let sit 10 minutes for air to settle, go to work.
flame your needle outside, and put the cap over it without touching it, if u touch the needle to anything flame it red again. then wipe it down and in it goes, no need for flame inside.
same thing if your working with a scalpel (i use a bent paper clip to manage my agar), flame outside, wipe down(iso) all but the flamed area, place inside.
yes u can spawn just about anything with a brf cake, just empty a colonized jar into a plastic sandwich bag and break it up.
pf cakes for fruiting suck, make a casing.
and genetics will be a problem if your doing it like you say.
what u want to do is save the culture u made from the first mushroom, and innoculating other plates with it to be used as spawn so the original stays growing in your culture library.
save that first culture!
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Phallus
I'd fruit that.
Registered: 07/22/08
Posts: 406
Loc: sunshine shit-land
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Re: Glove Box and Other Questions [Re: fltdriver82]
#9795319 - 02/14/09 01:56 PM (4 years, 3 months ago) |
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Quote:
fltdriver82 said:
http://www.shroomery.org/9457/Ghetto-Chamber-Tek
I used this method and line the top (were the top seals with the storage container) with that window door insulation that comes on a roll with one side being sticky. Sorry but i can't for the life of me think what that shits called.
Didn't use sewer rings ($7 each) just used 3" pvc pipe cut to about 6 inches ($1.50) and sealed with painters caulk and construction adhesive on both side.
I love how simple that is, I definitely need to give it more consideration.
Quote:
For birthing: yes also dunk and roll. The adv. to birthing is that instead of having one side to grow on the mushies can grow on top and all around the sides of the cake=more mushies.
Thought so, suppose it is obvious :p
Quote:
Don't wait for summer man, splurge the $20 to get a couple syringes and start now, plus you will know exactly what strain you have.
Well I'm in Florida, so it is only a few more weeks and I haven't gathered any supplies yet. I've been telling myself to start this for quite a while actually :3
Quote:
CptnGarden said:
flame your needle outside, and put the cap over it without touching it, if u touch the needle to anything flame it red again. then wipe it down and in it goes, no need for flame inside.
same thing if your working with a scalpel (i use a bent paper clip to manage my agar), flame outside, wipe down(iso) all but the flamed area, place inside.
What if one of the jars is something is already contaminated? I would spread it to everything else.
Quote:
pf cakes for fruiting suck, make a casing.
I'm just learning, I figured I should start small scale first. I don't really want to be overwhelmed with mushrooms, heh.
Quote:
genetics will be a problem if your doing it like you say.
what u want to do is save the culture u made from the first mushroom, and innoculating other plates with it to be used as spawn so the original stays growing in your culture library.
So I should never inoculate anything from any fruiting after the first?
Edited by Phallus (02/14/09 02:02 PM)
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: Glove Box and Other Questions [Re: Phallus]
#9795441 - 02/14/09 02:26 PM (4 years, 3 months ago) |
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for the original question, I wouldn't make a steel glove box, but you could do it. My glove box. I bought a clear 105qt tub, flipped it over, heated a coffee can and used it to make two holes. I could add a plexiglass lid for the sake of visibility and ease of placing objects inside, but right now I just lift the whole tub part off of the lid and put things in.
Flame the needle between each jar is what he was saying. That way if you contract a contam on the first jar, you will kill it before doing the second jar.
PF cakes DON'T suck for fruiting. I have seen great results from them. It depends on how you treat them.
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Phallus
I'd fruit that.
Registered: 07/22/08
Posts: 406
Loc: sunshine shit-land
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Re: Glove Box and Other Questions [Re: Shroominit]
#9795495 - 02/14/09 02:39 PM (4 years, 3 months ago) |
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Quote:
Shroominit said:
for the original question, I wouldn't make a steel glove box, but you could do it. My glove box. I bought a clear 105qt tub, flipped it over, heated a coffee can and used it to make two holes. I could add a plexiglass lid for the sake of visibility and ease of placing objects inside, but right now I just lift the whole tub part off of the lid and put things in.
If I had it fabricated I wouldn't have to pay for it, that's the only reason. But tubs aren't expensive and appear surprisingly effective.
Quote:
Flame the needle between each jar is what he was saying. That way if you contract a contam on the first jar, you will kill it before doing the second jar.
I can't do that if I keep the lamp outside the glove box, right?
Quote:
PF cakes DON'T suck for fruiting. I have seen great results from them. It depends on how you treat them.
The amount gained into the two methods I mentioned probably vary quite a bit.
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CptnGarden
fuck this site
Registered: 05/13/04
Posts: 11,945
Last seen: 4 years, 29 days
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Re: Glove Box and Other Questions [Re: Phallus]
#9795547 - 02/14/09 02:51 PM (4 years, 3 months ago) |
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the point of the glovebox is to let the air settle and work gently as not to rough it up!!!
if u have a flame in there, u will stir the air up, causing the contamns in there to be flying around, and into any openings on your jars/agar.
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Phallus
I'd fruit that.
Registered: 07/22/08
Posts: 406
Loc: sunshine shit-land
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Re: Glove Box and Other Questions [Re: CptnGarden]
#9795713 - 02/14/09 03:29 PM (4 years, 3 months ago) |
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I thought the point was to create an environment with the least amount of contaminations as possible, is sterilizing the air inside not an option?
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CptnGarden
fuck this site
Registered: 05/13/04
Posts: 11,945
Last seen: 4 years, 29 days
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Re: Glove Box and Other Questions [Re: Phallus]
#9795815 - 02/14/09 03:45 PM (4 years, 3 months ago) |
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it helps, but you cant sterilize 100%
theres going to be contamns in there no matter how much lysol/oust/isopropyl/hydrogen peroxide/bleach you spray.
focus on letting the air settle, and not trying to stir it around too much.
some people even add a little extention off the bottom, such as a wire rack with feet, as not to stir up the bottom too much with moving the jars around.
its funny people think its about nuking with chems, which is only a fraction of the sterility process. i dont wipe down that cardboard glovebox i just spray a couple shots of watered down Thymol into the box, let it settle, then go to work.
as for flaming the needle and bringing it back in, this is fine. just cap it without touching the needle (hard part, but ez with practice), wipe it/your hands down with isopropyl, and carefully add it back in.
if your worried about bringing new germs in, you can either give a few mists into your container with a hydrogen peroxide solution and let it settle, or just wait 5-10 minutes for the air to settle again.
i was in your shoes years ago, but ive had plenty of time to play around with glovebox setups since, and putting your lamp in the box is a bad idea, dont u keep a damp alcohol rag in there for wiping every minute or two? what if the fumes condense and go BOOM or the rag/your alcohol covered hands catches fire?
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: Glove Box and Other Questions [Re: CptnGarden]
#9795876 - 02/14/09 03:56 PM (4 years, 3 months ago) |
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I just take my hand out with the syringe/needle, flame and re-insert. Not slow, but not so fast that I stir up the air.
Metal is fine if you want to go the free route, I was just thinking of ease of moving. My GB is huge (3'x2'x2' ish) and I move it almost daily. It only weighs like 2 lbs though.
Now a steel flowhood would be the shit. 
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Stimpy913
Happy Happy Joy Joy
Registered: 11/05/08
Posts: 765
Last seen: 4 years, 1 month
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Re: Glove Box and Other Questions [Re: Shroominit]
#9795983 - 02/14/09 04:15 PM (4 years, 3 months ago) |
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Do not light a flame in a glove box. The hot flame will screw with the air pressure and the whole purpose of the glove box will fly out the window. Also most people spray down the box with lysol, or alcohal, these are very flammable. People here on the shroomery have had there arms burnt but flaming in glove boxes.
--------------------
How to talk to cops
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 13,519
Loc: UK 
Last seen: 1 hour, 5 minutes
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Re: Glove Box and Other Questions [Re: Shroominit]
#9795990 - 02/14/09 04:16 PM (4 years, 3 months ago) |
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Quote:
Phallus said:
I had a few questions about a glove box I intend to be making, according to other posts I've read, you fellows would call it a "still air" glove box. I used the design in Psilocybin Mushroom Handbook: Easy Indoor & Outdoor Cultivation, I just had a few questions and possible upgrades.
If I were to construct this out of steel instead of lining cardboard with contact paper, would it be easier to keep sterile? I ask this because I can easily have one fabricated, possibly cheaper than a 2'x 2'x 2' cardboard box.
I haven't decided on what I would be using for the clear covering yet, but how hot is it likely to get inside with an alcohol lamp burning? Do I need to worry about any melting?
I was also considering having rubber placed over the arm holes instead of flaps, and cut them into slices, so as to have numerous triangles covering the holes. This would reduce the amount of unsterilized air entering the environment. Is this worth doing, or is air coming through the arm holes not an issue?
I also have some other, general, questions about cultivating:
Could numerous colonized jars be placed into a tray and fruited, skipping the grain bag inoculation step? Once placed in the tray would the different colonies compete for nutrition, etc?
Can mycelium in the brown rice flour of a PF Tek be used to colonize a grain jar?
Is there a benefit to removing the cakes in a PF Tek and placing them in perlite instead of leaving them in the jars and blocking sunlight to the sides of the jar, inspiring fruiting? It appears to produce more fruits when the cakes are placed in perlite, but does it average out through flushes or is it more/less concentrated over the area available? I'll probably try both methods regardless, just curious.
How big of an issue is senescence, if I were to clone a cubensis from a PF Tek, colonize a grain jar, fruiting in a tray and then cloning one from the tray and repeating said steps would it cause a noticeable decline in quality/efficiency/productivity? If I used spores instead of cloning?
I'm really looking forward to starting, just waiting for spring to come into play so I can go pick some beauties to get spores from.
i typed a whole post and got distracted and now its gone, bt now i seen the various replys i will have to type it again.
A glovebox works on the principle of still air, anything inside will settle to the bottom and not get kicked into the air to land on anything. Do not use toxic chemicals, you dont even need to use alcohol or bleach or lysol. just soapy water, leaving the surfaces damp will aid keeping contaminants settled.
Do not use an alochol lamp inside. Just have it outside next to your glovebox, flame the needle well along the entire area, getting it red hot, then quickly move into your glovebox. Your needle wills stay hot enough in the time it takes to go inside that any contaminants that land wont survive.
Do thise before inoculating each jar, this is what prevents cross contamination.
I see no reason not to make it out of stainless steel, it will sure last a long time, and if it doesnt cost much great. As long as it breaks the wind fine :-)
I use a glovebox with no covering over the arm holes, its worked well for me. If you watch RR videos his glvoebox is the same, and he weres gloves with tyvek sleeves. After you flame sterilise your needle, you dont want it to touch anything, which could contaminate it and also cool it faster. Having something over your arm holes could make it very difficult
You still need a teerarium whether you fruit out of the jar on in. You need to surounding air to be high humidity otherwise fruits will dry out fast. Just birth them to a perlite shotgun fruiting chamber. works great
Yes continually will cause senescence. Theres only so many times a strain of mycelium can keep dividing cells. Im not sure how far you can go, its probably recomended only a few times.
--------------------
New Cultivators and members to the forums, please visit the following links which should answer alot of your questions.
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
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MushHunter08
Mycological Pupil
Registered: 06/09/08
Posts: 522
Loc: B.F.E
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Re: Glove Box and Other Questions [Re: Phallus]
#9796040 - 02/14/09 04:25 PM (4 years, 3 months ago) |
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Quote:
Phallus said:
I thought the point was to create an environment with the least amount of contamination's as possible, is sterilizing the air inside not an option?
The only thing that a still air glove box does is keep your work area draft free. The box itself doesn't even have to be sterile. I always just wipe down the inside of mine w/ alcohol and let it sit for about 10 mins after that before I go to work. Here is a video for you to look at so you can better understand the concept behind a glove box. Also my glove box isn't fancy either. It's just a clear plastic tote I punched two holes in the side w/ a small heated coffee can. Hope this clears some of your questions up. Here is the video link I was talking about.
--------------------
"The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough."
-William Blake-
The most simple method of growing mushrooms:
www.mushroomvideos.com
MultiSync's lazy bastard print/syringe guide
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Phallus
I'd fruit that.
Registered: 07/22/08
Posts: 406
Loc: sunshine shit-land
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Re: Glove Box and Other Questions [Re: Stimpy913]
#9796217 - 02/14/09 04:59 PM (4 years, 3 months ago) |
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Apparently I misinterpreted the purpose of the glove box, but with my new understanding I won't want gloves secured to the box; for that could become a huge pain when you are inoculating more than 2 jars.
Thank you all 
Quote:
MushHunter08 said:
Quote:
Phallus said:
I thought the point was to create an environment with the least amount of contamination's as possible, is sterilizing the air inside not an option?
Why did you do that? I went back to edit and didn't have to, because I didn't actually type it as such. Threw me off :3
Edited by Phallus (02/14/09 05:02 PM)
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MushHunter08
Mycological Pupil
Registered: 06/09/08
Posts: 522
Loc: B.F.E
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Re: Glove Box and Other Questions [Re: Phallus]
#9796362 - 02/14/09 05:35 PM (4 years, 3 months ago) |
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Do what exactly? Quote your post?
--------------------
"The road to excess leads to the palace of wisdom...for we never know what is enough until we know what is more than enough."
-William Blake-
The most simple method of growing mushrooms:
www.mushroomvideos.com
MultiSync's lazy bastard print/syringe guide
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Phallus
I'd fruit that.
Registered: 07/22/08
Posts: 406
Loc: sunshine shit-land
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Re: Glove Box and Other Questions [Re: MushHunter08]
#9796497 - 02/14/09 06:09 PM (4 years, 3 months ago) |
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Quote:
MushHunter08 said:
Do what exactly? Quote your post?
You changed "contaminations" to "contamination's"
Perhaps it was some automatic program on your computer?
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CptnGarden
fuck this site
Registered: 05/13/04
Posts: 11,945
Last seen: 4 years, 29 days
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Re: Glove Box and Other Questions [Re: MushHunter08]
#9796797 - 02/14/09 07:13 PM (4 years, 3 months ago) |
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i was going to attach gloves to the new one i just built, but after trying it out i really dont like attached gloves.
i use bare arms and latex gloves, both wiped well with 91% isopropyl.
heres the one that just finished drying, the silicone actually has a few more hours to cure but ive been using the glovebox for the past few hours.
cost me 30$ total,
rubbermaid tub
two 4" ABS closet flanges (the black rings - they are for bolting a toilet to the floor)
silicone sealant
and u cant see it in this pic too well but the syran wrap sticks to the silicone really well.
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