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 flugelizor
Furious ball of nothing
 
Registered: 11/16/08
Posts: 405
Loc: Western NY
Last seen: 2 days, 11 hours
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 contaminated agar+h2o2 close picture 
#9468179 - 12/20/08 07:02 PM (4 years, 4 months ago) |
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Heres a nice close up picture of shimeji mycelium contaminated with something creamy, not too liquid.
Looks almost like yeast.
I would say bacteria, but it is agar + peroxide.
The peroxide strength was tested and added just as the agar was starting to gel.
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solumvita
Q.B.E.
Registered: 02/12/08
Posts: 1,975
Loc: South Africa
Last seen: 12 days, 1 hour
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Re: contaminated agar+h2o2 close picture [Re: flugelizor]
#9471672 - 12/21/08 10:25 AM (4 years, 4 months ago) |
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around the edge of the ring of creamy "bacteria" looking slime there appears to be some mycelium which is growing low and on the surface? If the pink coloration is not from the shimeji then it looks a bit like a Fusarium contamination.
That creamy stuff looks more like bacteria than anything else, even though you say you used a peroxide solution, what strength did you make it?
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One of these days all the answers will be revealed until then we learn from each other!
www.mushrush.co.za
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flugelizor
Furious ball of nothing
Registered: 11/16/08
Posts: 405
Loc: Western NY
Last seen: 2 days, 11 hours
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Re: contaminated agar+h2o2 close picture [Re: solumvita]
#9471784 - 12/21/08 11:04 AM (4 years, 4 months ago) |
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I added 1ml 3% h2o2 to 150ml MYA
I was assuming the cottony myc was the shimeji.
But if not, then I may have a bad culture.
I think the shimmery effect around the outside of the growth on the right is just reflected light
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solumvita
Q.B.E.
Registered: 02/12/08
Posts: 1,975
Loc: South Africa
Last seen: 12 days, 1 hour
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Re: contaminated agar+h2o2 close picture [Re: flugelizor]
#9471811 - 12/21/08 11:16 AM (4 years, 4 months ago) |
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I also assume the cottony growth is the shimeji, i was looking at the pinkish discoloration mostly to the left of the plate.
How did you prepare the mya?
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One of these days all the answers will be revealed until then we learn from each other!
www.mushrush.co.za
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flugelizor
Furious ball of nothing
Registered: 11/16/08
Posts: 405
Loc: Western NY
Last seen: 2 days, 11 hours
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Re: contaminated agar+h2o2 close picture [Re: solumvita]
#9473753 - 12/21/08 06:47 PM (4 years, 4 months ago) |
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I'm a bit colorblind in the red & green spectrums.
I can usually see pink, but I don't here.
I destroyed this one, but next time I will ask my wife to help me spot any off colors on the plate.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 39,269
Loc: USA Mountain Northwest
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Re: contaminated agar+h2o2 close picture [Re: flugelizor]
#9474090 - 12/21/08 07:51 PM (4 years, 4 months ago) |
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Transfer from the leading edge of the mycelium to a new dish. The slime is bacteria.
I strongly disagree with using peroxide in petri dishes. It hinders the mycelium almost as much as the bacteria, thus your mushroom mycelium has two battles to fight. If bacteria is a problem, add 10mg to 20mg of Gentamicin sulfate per liter to the agar prior to sterilization.
It usually takes a series of four or five transfers to get pure, clean mycelium.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
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 tahoe
Noob Slayer
Registered: 11/27/03
Posts: 5,420
Loc: N38.93829W119.98108
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Re: contaminated agar+h2o2 close picture [Re: RogerRabbit]
#9475869 - 12/22/08 01:39 AM (4 years, 4 months ago) |
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Quote:
RogerRabbit said:
I strongly disagree with using peroxide in petri dishes. It hinders the mycelium almost as much as the bacteria, thus your mushroom mycelium has two battles to fight.
I am getting tired of this whole peroxide craze. These idiots use it for everything as if it even works.
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The subjects that I cover are not species specific and can be used in All Aspects of Mycology
I really need a good flow hood and a scope
Teh=The
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flugelizor
Furious ball of nothing
Registered: 11/16/08
Posts: 405
Loc: Western NY
Last seen: 2 days, 11 hours
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Re: contaminated agar+h2o2 close picture [Re: tahoe]
#9479740 - 12/22/08 09:00 PM (4 years, 4 months ago) |
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OK "Noob Slayer", but at least these idiots don't have grammatical errors in their signatures.
I really don't think my first try with agar+peroxide really qualifies as "using it for everything".
Edited by flugelizor (12/22/08 09:04 PM)
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 39,269
Loc: USA Mountain Northwest
Last seen: 1 day, 17 hours
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Re: contaminated agar+h2o2 close picture [Re: flugelizor]
#9479868 - 12/22/08 09:33 PM (4 years, 4 months ago) |
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I think new growers should get an extra dose of tolerance because this can be a tough hobby to learn, especially with all the conflicting information on this and other sites, as well as in books written on the subject.
RR
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
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tahoe
Noob Slayer
Registered: 11/27/03
Posts: 5,420
Loc: N38.93829W119.98108
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Re: contaminated agar+h2o2 close picture [Re: flugelizor]
#9480340 - 12/22/08 11:04 PM (4 years, 4 months ago) |
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Quote:
flugelizor said:
OK "Noob Slayer", but at least these idiots don't have grammatical errors in their signatures.
That is funny. Someone hacked my account last year and messed with all my shit. I have never gotten around to fixing it
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The subjects that I cover are not species specific and can be used in All Aspects of Mycology
I really need a good flow hood and a scope
Teh=The
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