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OfflineLacutice
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Smell
    #9418133 - 12/12/08 11:48 AM (3 years, 5 months ago)

Well, Im doing my first grow using BRF jars and I think I have a problem.

I PCed 12 half pint jars [6 at a time for about 1 hour at 15 psi] and feel I did everything right. I was very clean, my jars were wiped down before applying a dry verm layer, and they were kept dry using foil while in the PC.

They were allowed to sit for about 8 hours until fully cool at which point they were inoculated using B+ spores, inside a glovebox. I did 3 at a time inside the box, flaming the needle red hot and wiping down with alcohol in between.

They were placed in a closet that had been sprayed down with lycol and was clean to begin with. The inoculation holes were left open to allow for GE as I had applied a dry verm layer.

Its been about 36 hours since inoculation and they have been sitting in the closet at 76-79f. I took a look and they seem fine [no growth, mycelium or otherwise from what I can see] but I took a smell near the inoculation holes and they all seem to have an almost sweet smell.

The smell is very light, and hasnt made the closet or anything smell [though I sprayed lycol before opening it]. Ive tried searching around and have found nothing conclusive. Some people report PCed BRF jars having a smell, but I did not smell them out of the cooker or anything so Im unsure. Many people have reported a slightly sweet smell in other posts but I have found no conclusions, just people wondering and speculating.

So is it normal for a BRF jars to have a smell if you take a sniff near the inoculation holes?

Perhaps I got screwed on my spores. Unfortunately I did not know about shroomery and its sponsors and ordered from Spore Lab. It took 3 weeks after payment was received to get my spores, and I only got 3 of the 5 I paid for. He claims my order was shipped separately.

Is it possible that Captain Max has sold me useless syringes? I guess only time will tell.

Im gonna let the jars sit for a couple days and Ill keep an eye on the inoculation sites, watch for contamination. I know my procedure was good, I dont think I could have messed up 2 separate PCs or contaminated 12 jars that were inoculated in 4 isolated groups using sterilized equipment.

Or maybe Im just getting ahead of myself. Any thoughts?


Edited by Lacutice (12/15/08 02:16 PM)


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OfflineDamion5050
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Re: Smell [Re: Lacutice]
    #9418175 - 12/12/08 11:53 AM (3 years, 5 months ago)

Well first off it sounds like you did everything good.. :thumbup: 

Second.. Takes 3-7 days for the spores to germinate.. So that means there could be no signs of growth for the first week..

Third.. Don't smell it you are over paranoid.. Just give it time..

PATIENCE IS THE KEY TO THIS HOBBY


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OfflineLacutice
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Re: Smell [Re: Damion5050]
    #9418220 - 12/12/08 11:59 AM (3 years, 5 months ago)

Well I certainly wasn't expecting any growth, I just thought a checkup was in order and for some reason smelling came into play. I smelled one, and by the end I had smelled them all and was thinking, CONTAMINATION!

So I locked them up, spread them out some more and haven't breathed near the closet since.

Oh, I also made a honey LC which was inoculated about 12 hours ago [PC'ed 20 minutes at 15 PSI]. If there is a problem with the spores would this help act as an early indicator? I mean, would the LC be susceptible to the same contamination as BRF/verm if the syringe was contaminated?


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Re: Smell [Re: Lacutice]
    #9418249 - 12/12/08 12:03 PM (3 years, 5 months ago)

It certainly wouldn't smell bad after 36 hours already. Just like the desired grow, contam needs its time to take a hold, too. And while that certainly COULD have happened after 36 hours, it's very unlikely that the jars would already smell funny.


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Re: Smell [Re: Damion5050]
    #9418265 - 12/12/08 12:04 PM (3 years, 5 months ago)

If things start to smell really sweet you could be in trouble that's fermentation that means bacteria is kicking some ass and taking names.

My best advise is to leave those bitches alone. You're most likely fine. I didn't even clean my jars before putting things in them and putting them in a PC and you know what happened...They all came out fine!

It's very strange to see growth in the first two days. My first batch had massive growth with in the first 12-24 hours (this is really rare). I mean fur balls of Myc were everywhere. My second jars didn't show growth for at least a week and then thing exploded from from there. If you don't see growth in the first week or more then you should start to worry a bit.

I say you should... :jah: and relax. I bet your fine! Worry when you start to see and smell weird shit.


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Edited by ronjohn7779 (12/12/08 12:07 PM)


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Offlinedrjugglz
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Re: Smell [Re: ronjohn7779]
    #9418328 - 12/12/08 12:12 PM (3 years, 5 months ago)

smells like fermentation.

with honey lc you wont be able to distinguish a contam from mycelium. although most lc's that ive seen contam'ed turned cloudy at the bottom. then when shaken it was almost like smokey. huge difference between white fluffy mycelium clouds.


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OfflineLacutice
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Re: Smell [Re: drjugglz]
    #9418733 - 12/12/08 01:04 PM (3 years, 5 months ago)

What would cause the fermentation? I think I was pretty good with following the pf-tek and getting proper moisture [1 cup water to 2 cups verm them 1 cup BRF after hydrating the verm] so I don't think the moisture was off.

I understand the concept of fermentation I'm just wondering what somebody could have done to their jars [or failed to have done] to cause it.

But hopefully all is well.


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OfflineDamion5050
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Re: Smell [Re: Lacutice]
    #9418757 - 12/12/08 01:07 PM (3 years, 5 months ago)

All should be well if you pced them for as long as you say you did then you should be good to go.. Give them alittle more time.. And don't concentrate so much on there smell..


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Invisibledjmako7
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Re: Smell [Re: Damion5050]
    #9418799 - 12/12/08 01:14 PM (3 years, 5 months ago)

"The inoculation holes were left open to allow for GE as I had applied a dry verm layer." - Lacutice

What?:rolleyes:

Did you put micropore tape on or any other filteration methods?

And for the captain max comments sporelab is my trusted vendor and every syringe I got has worked.


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Edited by djmako7 (12/12/08 01:19 PM)


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OfflineDamion5050
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Re: Smell [Re: djmako7]
    #9418861 - 12/12/08 01:22 PM (3 years, 5 months ago)

Quote:

djmako7 said:
"The inoculation holes were left open to allow for GE as I had applied a dry verm layer." - Lacutice

What?:rolleyes:

Did you put micropore tape on or any other filteration methods?

And for the captain max comments sporelab is my trusted vendor and every syringe I got has worked.




He is using the Pf tek in which case he doesn't need a filtration layer.. Because the dry vermiculite layer is the filtration layer..


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Invisibledjmako7
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Re: Smell [Re: Damion5050]
    #9418920 - 12/12/08 01:31 PM (3 years, 5 months ago)

Quote:

Damion5050 said:


He is using the Pf tek in which case he doesn't need a filtration layer.. Because the dry vermiculite layer is the filtration layer..




And micropore tape right? The layer of verm helps add a barrier but is not 100 percent. I personally have never seen anyone post that they do not use a filtration method for the holes on the lid.

Jars will have a higher chance of contam without it. Next time fork over the 3 bucks and buy some micropore tape.


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OfflineDamion5050
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Re: Smell [Re: djmako7]
    #9418978 - 12/12/08 01:40 PM (3 years, 5 months ago)

Micro pore tape is not necessary. Unless he thinks it is..  I never use it and I have never had a cake contaminate..  The original PF TEK by RR doesn't even say to use it..

Here look 2 pint cakes 4 days after inoculation.. No Tape just a dry verm layer..  If you have a cake contaminate with a dry verm layer that means your sterility is at fault or a bad syringe, not the technique..






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Re: Smell [Re: djmako7]
    #9419019 - 12/12/08 01:45 PM (3 years, 5 months ago)

I dont use any layer other than the verm. Out of more than 40 jars I have done so far I have only lost 1 to contam.
RR's video only uses the dry verm layer for PF Tek. He should be fine with just the verm as long as his procedures were correct; which it sounds like they are.
As for not using a vendor, I think alot of us have gone through that heartache. I know I did before I found the shroomery. Anyway, Lacutice, from one newbie to another. Good luck with your grow.


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OfflineDamion5050
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Re: Smell [Re: schizophrenic]
    #9419026 - 12/12/08 01:46 PM (3 years, 5 months ago)

Quote:

schizophrenic said:
I dont use any layer other than the verm. Out of more than 40 jars I have done so far I have only lost 1 to contam.
RR's video only uses the dry verm layer for PF Tek. He should be fine with just the verm as long as his procedures were correct; which it sounds like they are.
As for not using a vendor, I think alot of us have gone through that heartache. I know I did before I found the shroomery. Anyway, Lacutice, from one newbie to another. Good luck with your grow.




:thumbup:  Yeah I had a bad experience with a bad vendor before I found this forum.. Spent 40 bucks on bunk syringes..


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Invisibledanielx
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Re: Smell [Re: Damion5050]
    #9419204 - 12/12/08 02:20 PM (3 years, 5 months ago)

hey Lacutice, i sent you a pm. check it.


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Invisibledjmako7
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Re: Smell [Re: danielx]
    #9419362 - 12/12/08 02:54 PM (3 years, 5 months ago)

its the guys first grow. Better to be safe than sorry.


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OfflineLacutice
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Re: Smell [Re: djmako7]
    #9423834 - 12/13/08 11:15 AM (3 years, 5 months ago)

I have micropore tape, I planned on using it but was told by a couple people not to bother. They said it would simply impede GE and offer no real benefit over the dry verm layer. One actually told me he was using micropore tape and his jars had no action until he pulled the shit off and then they started up like crazy.

I'm trying to stay away, I'll just let them sit until Monday before I even look at them. Hopefully I'll see nothing more than a little myc [if that] and no smell.

We'll see if this Captain Max guy decides to do right by me. He certainly hasn't shown it yet by withholding 2 of the 5 syringes I paid for.

However, any word on what I may have done wrong to cause fermentation? If there is a problem, and that is it I would like to avoid it in the future. I just can't identify anything I did that was out of the ordinary. I didn't add anything, or change the recipe in anyway. The moisture was all good from the looks of it. I just don't want to have any problems if I'm forced to do this again.


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OfflineLacutice
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Re: Smell [Re: djmako7]
    #9423844 - 12/13/08 11:17 AM (3 years, 5 months ago)

I have micropore tape, I planned on using it but was told by a couple people not to bother. They said it would simply impede GE and offer no real benefit over the dry verm layer. One actually told me he was using micropore tape and his jars had no action until he pulled the shit off and then they started up like crazy.

I'm trying to stay away, I'll just let them sit until Monday before I even look at them. Hopefully I'll see nothing more than a little myc [if that] and no smell.

We'll see if this Captain Max guy decides to do right by me. He certainly hasn't shown it yet by withholding 2 of the 5 syringes I paid for.

However, any word on what I may have done wrong to cause fermentation? If there is a problem, and that is it I would like to avoid it in the future. I just can't identify anything I did that was out of the ordinary. I didn't add anything, or change the recipe in anyway. The moisture was all good from the looks of it. I just don't want to have any problems if I'm forced to do this again.


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Invisibledjmako7
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Re: Smell [Re: Lacutice]
    #9424495 - 12/13/08 01:13 PM (3 years, 5 months ago)

Quote:

Lacutice said:


We'll see if this Captain Max guy decides to do right by me. He certainly hasn't shown it yet by withholding 2 of the 5 syringes I paid for.






I m sure if you email him with your concern he will reply. I emailed him yesterday and got a reply today. They had a little hiccup back in november so they are trying to play the catch up game.

You stated "I just can't identify anything I did that was out of the ordinary."

Watch RR's video on how to make BRF cakes. Follow every step. And im sure you will narrow your problems. The fact that you are a beginner you should take all the precautions you can rather than half ass. Or make 12 jars half of em cover with tape half of them dont. Go on which has better results for you.
Just trying to help out, I know what a bummer contams can be.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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