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 troncotron
Stranger
Registered: 12/13/08
Posts: 75
Loc: Spain
Last seen: 3 days, 15 hours
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 semilanceata attempt
#9423325 - 12/13/08 08:10 AM (3 years, 5 months ago) |
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Hello, this is my first attempt with semilanceata. I took some prints from a really big specimen found in Spain at an altitude of 1600m. First i germinate the spores on PDA media and watched with the microscope. I could see spores very similar to cubensis.
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troncotron
Stranger
Registered: 12/13/08
Posts: 75
Loc: Spain
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Re: semilanceata attempt [Re: troncotron]
#9423347 - 12/13/08 08:18 AM (3 years, 5 months ago) |
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Then i isolate a strain and inoculate some birdseed jar. Now, 12 days after, the micelium looks weak and its growing slowly. If any of you has grown it before, do it seems to be real psilocibe semilanceata micelium??
thank you, bye
TT
  
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 AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 337
Loc: Seti Alpha 5
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Re: semilanceata attempt [Re: troncotron]
#9423453 - 12/13/08 08:59 AM (3 years, 5 months ago) |
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Greetings and Happy Holidays to you. It's good to see someone attempting to grow P. semilanceata. I know that they're really difficult to grow. Never the less, I do have a suggestion. When your culture does produce fruiting bodies, collect the spores and take them through successive generations. In theory, as you repeat your growing technique on successive generations of P. semilanceata, you'll be selecting a strain that responds best to your cultivation technique. In essence, after each generation, it should get easier and easier to grow them.
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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Libertycapper
Doctor Banner
Registered: 11/13/04
Posts: 579
Loc: British Columbia 
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ive been trying to get them to grow outdoors for a while now. have had a bit of success with the mycellium but have not fruited one so far. good luck!
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denger
Mycelium keeper
Registered: 09/19/08
Posts: 712
Loc: MD, US
Last seen: 1 year, 7 months
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One possible reason for slow growing mycelium is it could be haploid. When you plates spores, how many germination points did you see?
If you still have the original dish, I would recommend making a few more isolates from it. Also, on a first round a multispore cake ala pf-tek would possibly give a better result. You can clone good specimens from the cake if you manage to fruit it.
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Dennis, in Love with Fungi
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek
Looking for Chantarelle and Armillaria cultures, have a huge collection of other edibles to trade.
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troncotron
Stranger
Registered: 12/13/08
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Re: semilanceata attempt [Re: denger]
#9424291 - 12/13/08 12:41 PM (3 years, 5 months ago) |
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hello, thanks for the answers. Denger,i first supposed what you said, it seems to be haploid, but took some pictures to a little piece of the plate when the micelium started to appear, and i saw some spores germinating (the pic isn´t very clear, but they look like germinating). The first picture shows the original plate with different strains. To be sure about plasmogamy, i inoculated the jars with three or four strains.It is possible that still they havn´t been and fused , cause the micelium in the plate now shows more vigor than in the jar. I´ll try to "rescue" the print and make a multispore dissolution. I will update..
 
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Alan Rockefeller
Mycologist
Registered: 03/10/07
Posts: 24,722
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Re: semilanceata attempt [Re: troncotron]
#9427118 - 12/13/08 08:44 PM (3 years, 5 months ago) |
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Quote:
Now, 12 days after, the micelium looks weak and its growing slowly. If any of you has grown it before, do it seems to be real psilocibe semilanceata micelium??
Looks good to me, that is exactly what I would expect.
Do you have a refrigerated fruiting chamber?
I have always wondered if its possible to make a fruiting outdoor patch in a lawn or sawdust.
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troncotron
Stranger
Registered: 12/13/08
Posts: 75
Loc: Spain
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Now the jars are to approximately 20 degrees (i think it´s a bit high) but when it´s totally colonized, i´ll let it fruit at 8-12ºC putting the fruiting chamber between a double window.
regards
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 11,658
Last seen: 1 month, 2 days
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Re: semilanceata attempt [Re: troncotron]
#9435646 - 12/15/08 08:34 AM (3 years, 5 months ago) |
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Really interested to see this play out.
I seen a log of someone that got liberty caps to fruit, but it wasnt many and i think the project got abandoned.
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Roger Rabbit and Road Kill cultivation videos Starting out-This site will answer a lot of questions, great pf tek video guide
Agar's Grain LC Tek
Lipas Deli cup Agar Tek
Cakes suck for pins - click me do it right
Spice - 
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Juke Adro
I love peach fluff
Registered: 04/05/08
Posts: 5,768
Loc: Inside your head
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Re: semilanceata attempt [Re: veda_sticks]
#9438783 - 12/15/08 05:17 PM (3 years, 5 months ago) |
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Yeah me too, as I'm about to also start cultivation too, so where you fail I may gain hehehehehe
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Someone said: im actually not using ms, im using prints.
  
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tripchip
The Mushroom Monkey
Registered: 07/06/08
Posts: 1,145
Loc: African Jungle
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Re: semilanceata attempt [Re: Juke Adro]
#9441635 - 12/16/08 01:02 AM (3 years, 5 months ago) |
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has anyone eaten this species before? If so, is it really as potent as they say it is? like a gram can blow your mind? just curious. Im going to study this species sometime.
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: semilanceata attempt [Re: tripchip]
#9441744 - 12/16/08 01:35 AM (3 years, 5 months ago) |
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I've read about them, apparently they're more potent than cubes - but not as potent as Azures or Pan Cyans. The trip is different too, and from what I remember reading, some ppl like them better than cubes.
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troncotron
Stranger
Registered: 12/13/08
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Loc: Spain
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Re: semilanceata attempt [Re: J3illy]
#9442213 - 12/16/08 05:43 AM (3 years, 5 months ago) |
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Hello!, I have eaten several times in quantities of 0,5g (approximately 20 units) and I have liked them very much. I agree in that they are more potent, but also more controllable, in my opinion.
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obi
Du Bois
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Re: semilanceata attempt [Re: troncotron]
#9502111 - 12/27/08 12:01 PM (3 years, 4 months ago) |
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^ Agreed, I prefer them to cubensis, haven't tried cyans/azurescens yet...
I wonder if it has something to do with them being native to my area.
@ troncotron; keep us updated! nice work 
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To live is to fly
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German Kahuna
Facepalmer of Stoopid
Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Re: semilanceata attempt [Re: obi]
#9502503 - 12/27/08 01:05 PM (3 years, 4 months ago) |
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Dried Ps. semilanceata are almost about twice as potent as dried cubes. Their biggest advantage is that they contain close to zilch psilocin, of which about 50% is lost during the drying process due to oxidation, but a shitload of psilocybin, which is much more resistant to oxidation with only about a 20% loss. That's why you can basically store liberty caps forever without much potency loss. 1 1/2 - 2 grams dry will whip your ass real good, too.
If you chose to eat them fresh, you'll also benefit from the highest level of baeocystin (plus norbaeocystin) found in any known psilocybe mushroom, a substance that is said to add to the visual aspect of the trip a great deal.
Unfortunately, semilanceata is also one of the Psilocybes resisting cultivation the most, which is why I consider myself lucky to live in an area where I can find them in the wild easily. What can I say... I've been in love with them for 20 years and my last wish is probably going to be for a baggy of libs to be dropped on my coffin before they shovel the dirt in. 
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"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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troncotron
Stranger
Registered: 12/13/08
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Loc: Spain
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This is how the jars are going. The birdseed one is about 60% colonized. The other jar (sterilized cow manure with birdseed) is working better (about 90% colonized). In one or two weeks i´m planning to do the casing layer with pasteurized garden soil.
 
Edited by troncotron (01/09/09 09:29 AM)
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inski
Cortinariologist
Registered: 02/28/06
Posts: 2,551
Loc: New Zealand 
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Re: semilanceata attempt [Re: troncotron]
#9578502 - 01/09/09 04:54 PM (3 years, 4 months ago) |
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They look good, are you casing these in the jars, or are you using trays?
I think I would only case the manure and birdseed jar and use the grain jar for g2g if I was certain there were no contaminants!
I love the microscopy of the germinated spores!
Good luck from New Zealand
inski.
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troncotron
Stranger
Registered: 12/13/08
Posts: 75
Loc: Spain
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Re: semilanceata attempt [Re: inski]
#9581410 - 01/10/09 03:07 AM (3 years, 4 months ago) |
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hey insky;), i think i´ll case in the jar, placing it inside the fruiting chamber at about 50ºF. what do you mean with g2g? suppose you mean using as a "master" to inoculate other jars.. in this case its a good idea, i´ll also do a LC to keep the mycelium and colonize more substrate.
I read here something very interesting that Workman said about the casing:
"They grow much slower and at lower temperatures. Some sort of refrigerated environmental chamber and a lot of patience are needed. They also don't fruit without a biologically active casing which can cause contamination problems if not well balanced."
I don´t know what he means with balanced casing. I´ll try to take soil from a pasture near i took the specimen, and do a low Tª pasteurization (140-150ºF for about two hours) to keep these favorable organisms. Am I right?:)
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German Kahuna
Facepalmer of Stoopid
Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Re: semilanceata attempt [Re: troncotron]
#9581454 - 01/10/09 03:40 AM (3 years, 4 months ago) |
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g2g = "grain to grain". Using one colonized jar as a master to inoculate like 8-10 more jars to increase the amount of grain spawn exponentially. I am really interested in the further development of this project. Good luck!
I think I'll make some lib prints myself when I go hunting this fall. Our cellar should have ideal FC conditions for libs.
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"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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nightflyer
Registered: 08/08/08
Posts: 392
Loc: Central Europe
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In-vitro cultivation of Psilocybe semilanceata by J. Gartz.
An apparently successfull substrate mixture:
Grass seeds, horse dung, brown rice grains and water.
http://
Edited by nightflyer (01/26/09 11:50 PM)
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