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Offlinedead
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Registered: 08/28/08
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Re: SUPER MEGA MUSHROOM? [Re: Mr.Winkie]
    #9226808 - 11/11/08 10:04 PM (4 years, 6 months ago)

Just do lots and lots of multispore grows on cakes, and when you come across a strain that fruits large on a cake, clone and isolate it and grow it on a better substrate. Then take the largest fruits you get and clone and isolate them.


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"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."

Links:
:regularshroom:Nibin's Guide for Noobs
:regularshroom:some easy teks on bulk & grain prep. (my journal)


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Invisibleseven
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Re: SUPER MEGA MUSHROOM? [Re: dead]
    #9226873 - 11/11/08 10:16 PM (4 years, 6 months ago)

i was speaking of doing side by side grows of the same strain from 2 diferent sources.keep both separate over many generations using spores from the largest fruit. once you notice a generation with all large fruits in both grows.  mix the largests spore prints from both grows togather. the keep it goin as one grow. mixing the prints would revive  vigor in the grow by adding variation:of the large kind:.. . no isolation yet.  isolate when you reach your goal of a monster fruit. find the substrain responsable for the large gennnetics in the fruit and you will have a monster isolate.  we werent talking about mixing diferent strains.


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grind


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OfflineStimpy913
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Re: SUPER MEGA MUSHROOM? [Re: dead]
    #9227854 - 11/12/08 01:04 AM (4 years, 6 months ago)

Quote:

dead said:
Just do lots and lots of multispore grows on cakes, and when you come across a strain that fruits large on a cake, clone and isolate it and grow it on a better substrate. Then take the largest fruits you get and clone and isolate them.




There's more nutrients in brown rice than in poo or coir or any of that stuff. Shrooms are just smaller on cakes because cakes aint that big.


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OfflineMr.Winkie
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Re: SUPER MEGA MUSHROOM? [Re: Stimpy913]
    #9228757 - 11/12/08 07:29 AM (4 years, 6 months ago)

seven. perfect. exactly. cant wait. stoked. I'll take pics. keep posted. procrastinate.





PROCRASTINATORS UNITE!!!!!...............................tomorrow:crazy2:


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PROCRASTINATORS UNITE!!!!.......................................................tomorrow


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OfflineEmperor Reishi
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Re: SUPER MEGA MUSHROOM? [Re: seven]
    #9228996 - 11/12/08 10:02 AM (4 years, 6 months ago)

Quote:

seven said:
i was speaking of doing side by side grows of the same strain from 2 diferent sources.keep both separate over many generations using spores from the largest fruit. once you notice a generation with all large fruits in both grows.  mix the largests spore prints from both grows togather. the keep it goin as one grow. mixing the prints would revive  vigor in the grow by adding variation:of the large kind:.. . no isolation yet.  isolate when you reach your goal of a monster fruit. find the substrain responsable for the large gennnetics in the fruit and you will have a monster isolate.  we werent talking about mixing diferent strains.




I think this would be a great project. The only problem I see is that it's not as easy to cross the two strains as just putting the spores from the two sources into the same media. They may mate, but you'de never know for sure, especially with a swab of spores.

I'm sure others here know much more about it then me, but a method I've read about and plan on trying is making dilutions of the spores. You would take a small amount of spores, and mix it with say 10cc (1 syringe worth) of sterile water... You would then use a few drops of that to mix a whole other syringe. From there you would streak some of the secondary diluted spore water over some agar plates.

I commented on this once before in a little more detail here:
http://www.shroomery.org/forums/showflat.php/Number/8932110#Post8932110

And I found the book online (full version and all I was surprised) You can read about it here:

Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home

p.340 Spore Dilution Technique:

http://www.scribd.com/doc/6652468/Mushroom-Cultivator-A-Practical-Guide-to-Growing-Mushrooms-at-Home


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EmperoR ReishI

Looking for:    Live Cultures: Maitake[sclerotia forming], Wood Ear[Auricularia Polytricha],            Prints: **Psilocybe mexicana,                                Reishi [other than red], Bioluminessent                                                                          Cubes & Pan Cyans

Available for trade:    Live cultures: Reishi[ganoderma lucidum], Shiitake[lentinula edodes], Chicken of the woods[Laetiporus                                            sulphureus], Enoki [Flammulina velutipes], Oyster [Pleurotus ostreatus], Lion's Mane [Hericium                                                            erinaceus]


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Invisibleseven
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Re: SUPER MEGA MUSHROOM? [Re: Emperor Reishi]
    #9229160 - 11/12/08 11:04 AM (4 years, 6 months ago)

seems like having equal portions of each source of spores in a regular syringe (non-diluted but not dark) and inoculated into a grain jar would give highest chances of mating between the two sources of the same strain. :shrug:


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