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Spores from the same print are the norm. There's plenty of genetic diversity within a sporeprint. Rhizomorphic mycelium is strain, therefore genetic related. When isolating strains, isolate rhizomorphic sectors. Not all rhizomorphic sectors will be good producers, but nearly all cottony sectors will be poor producers.

Yes, rhizomorphic mycelium is a good sign that the hyphae have mated. Monokaryons won't configure into rhizomorphs.
RR

Actually, a drier substrate will result in more rhizomorphic growth, as well as faster growth.  Your observation was correct.
RR

ve made numerous transfers of cottony mycelium and it stayed cottony. I've made numerous transfers of rhizomorphic mycelium and it stayed rhizomorphic. I believe there's more involved than we currently know. I have many strains that I've isolated down to single sector rhizomorphs, and these remain rhizomorphic even years later when removed from the refrigerator and transferred to new substrates. I think a lot of confusion comes from ceasing to make transfers before getting down to single sector isolates, thus the process of anastomosis is still occurring which would explain the mycelium changing to different types.

I've found a difference in appearance based on the nutritive substance in the growing medium, with the strands much closer together when highly nutritive media was used, and the strands spread out more when lesser nutritive media was used, but they're still rhizomorphs when viewed under the microscope, even if they don't look so to the unaided eye.

I've attempted to fruit completely cottony isolates and the results were also the same every time. Either they didn't fruit at all, or fruited poorly. Rhizomorphic isolates have almost always produced nice fruitings with only one or two exceptions out of hundreds. I go through about 500 petri dishes per month, so believe me when I say I've repeated these experiments many times over.
RR

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