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 nitricoxide
Stranger
Registered: 10/15/08
Posts: 3
Last seen: 3 years, 6 months
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 How does shop shiitake and source naturals 16 mushroom mix affect nitric oxide? 
#9080503 - 10/15/08 08:58 AM (3 years, 7 months ago) |
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Hi. I wish to increase glutathione while reducing nitric oxide/peroxynitrite/superoxide in my body. Is it okay to consume shiitake mushrooms from the supermarket, and source naturals mushroom immune defense, or will either one negatively affect this nitric oxide cycle for me? From reading below it appears keeping the mushrooms/capsules at room temperature destroys it, can you explain? Is it safe to consume in a home made sublingual/buccal mix? Thank-you so much, I wasn't sure where else to ask.
The immunomodulating action of an antitumor polysaccharide, lentinan, from Lentinus edodes was investigated in peritoneal maerophages of female BALB/c mice. First, the relationship between lentinan content, as measured by enzyme-linked immunosorbent assay (ELISA). and the effects on the production of tumor necrosis factor-α (TNF-α) and nitric oxide (NO) during storage was examined. When the mushrooms were stored at low temperature, the contents of their ami tumor polysaccharides and the production levels of TNF-α and NO showed hardly any changes. However, polysaccharide content and cytokine production decreased markedly at higher temperature (20°C). These results suggest that low-temperature storage is more effective in maintaining levels of antitumor polysaccharides and the quality of the mushrooms as the health-beneficient food. Next, the mechanism of cytokine production in murine macrophages stimulated with purified lentinan was examined. The results of time course experiments on the production of TNF-α and NO indicated that TNF-α was produced 12 h earlier than NO. An anti-TNF-α antibody inhibited completely NO production when added simultaneously with purified lentinan to macrophages. NOC-18 (NO donor) itself did not increase levels of TNF-α production. Moreover, simultaneous treatment with L-N-monomethylarginine (NO synthase inhibitor) and lentinan did not affect lentinan-induced production of TNF-α. The NO from macrophages was produced by an autocrine pathway through production of TNF-α.
http://www.begellhouse.com/journals/708ae68d64b17c52,4cf0547d63409c36,7c85d36656ddff8c.html
http://www.iherb.com/ProductDetails.aspx?c=1&pid=1472&at=0
The immunostimulant and immunomodulatory activity of both mycelial and fruiting bodies of ABM using water and ethanol extracts have been demonstrated in many in vitro experiments, although not always the results are concordant, but sometimes contradictory. Water extracts of the mycelial culture and fruiting bodies such as fractions B-4, B-5 obtained from ethanol precipitation (respectively 44% and 50%) of fruiting bodies, markedly induced TNF production and IL-8 of macrophages derived from rat bone-marrow (23). Fraction B-5 induced a significant increase in nitric oxide production, (23). In another paper, the same group using ethanolic fractions obtained from mycelia inhibited the occurrence of the viral cytopathic effect induced by Western equine encephalitis and herpes simplex (25). Other extracts containing lignin-based derivatives have shown the induction of TNF-, IL-8 and nitric oxide secretion by macrophages (26), anti-viral activity of different viruses (27), and direct anticancer activity (28); so lignin derivatives apparently have more different and important pharmacological activities. While, on the contrary, a down-regulation of IL-2, IL-4 and INF- in human peripheral blood mononuclear cells has also been documented (27). A hemicellulase-treated ABM fraction derived from mycelia composed of 63.3% carbohydrates, 30.9% proteins, 0.3% lipids and other minor components has shown to stimulate immature dendritic cell obtained from mice bone-marrow and up-regulate the expression of costimulatory molecules and MHC antigen, although did not increase the production of inflammatory inducible cytokines (30). ABM pre-treated dendritic cells inhibited some bacteria-mediated dendritic cells’ responses, ABM pre-treated macrophages reduced LPS-induced NF-B activity, while ABM-mediated dendritic cells enhanced the Th1 response in allogenic mixed lymphocyte reaction. These antithetical effects may probably help to maintain immunological homeostasis, so the question is: can they be clinically effective in cancer patients? (30). Fine particles of ABM fruiting body and mycelium, respectively, prepared by mechanical disruption, activated the human complement system via the alternative pathway in human serum, is another proof of its activity in enhancing natural immunity in bacterial infections (31). From these studies it is clear that the ABM fractions act on many different biological receptors of the immune system but can also have antithetical pharmacological activity; so further studies are warranted to completely identify the real importance of this mushroom as an immunostimulant and/or immunomodulator.
ABM Extracts as Anticancer Agents
Aqueous extracts of ABM given in the drinking water to rats and mice before chemical cancer induction exhibited antimutagenic effects, but were ineffective when administered in the post-induction period demonstrating protection only in the initiation step of liver carcinogenesis (37,68,69). These studies are of particular interest because the extraction consisted in preparing a ‘crude aqueous extract’ leaving a powdered dry fruiting body in water at room temperature for 2 h, which is the way ABM is popularly prepared (69).
Interestingly, rats fed with a dry powdered form of ABM using two strains (99/26 and 99/29) and prepared from two different moments of harvest (opened or closed basidiocarp) at 10% of the diet, on the basis of the strain and harvest (the more effective was strain Ab 26 closed basidiocarp) exhibited different significant antimutagenic activity, more evident when considering the reduction of both size and number of the preneoplastic lesions, even when given in the post-initiation period (70). Moreover, not only polysaccharides but also the lipid fraction of ABM was found to contain a compound with antitumor activity, subsequently identified as ergosterol (a precursor of ergocalciferol), that inhibited tumor-induced neovascularization in sarcoma 180-bearing mice by oral administration for 20 days without side effects, though the extract had no cytotoxic effect in vitro (71). Kimura (72) has identified from the lipid fraction sodium pyroglutamate, which has shown not only to have antiangiogenic (inhibition of von Willebrand Factor expression in tumors) and antitumor activity in Lewis lung carcinoma-bearing mice but also an inhibitory effect on the cancer-induced reduction of immune functions.
Ethanol fractions obtained from hot-water extract of mycelium or dried fruiting induced TNF- and IL-8 secretion in rat bone-marrow macrophages. Further fractionation with increasing ethanol concentrations resulted in the reduction of this cytokine-inducing ability in mycelial extracts, but enhanced it in fruiting body extracts. Whereas mycelial fractions did not induce nitric oxide production, fractions obtained by precipitation of fruiting-body extract with high ethanol concentrations stimulated macrophages to produce significantly higher levels of nitric oxide than controls (23).
In a mouse model of peritonitis induced by i.p. injection of fecal stem solutions the pre-challenge oral administration of an aqueous ABM extract protected mice against lethal septicemia after fecal peritonitis as demonstrated by a reduction in bacteremia and increase in survival rate, which was comparable with the survival of a verum group to which were administered per os metronidazole and doxycicline (73); and confirming previous results of the same group in mice given i.p. infection with the virulent Streptococcus pneumoniae serotype 6B (74).
http://ecam.oxfordjournals.org/cgi/content/full/nem007v1
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Juke Adro
I love peach fluff
Registered: 04/05/08
Posts: 5,768
Loc: Inside your head
Last seen: 1 year, 8 months
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Re: How does shop shiitake and source naturals 16 mushroom mix affect nitric oxide? [Re: nitricoxide]
#9084987 - 10/16/08 12:47 AM (3 years, 7 months ago) |
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Can I direct you to someone who can help? if so PM This guy with what you just said -----> curenado
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Someone said: im actually not using ms, im using prints.
  
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nitricoxide
Stranger
Registered: 10/15/08
Posts: 3
Last seen: 3 years, 6 months
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Re: How does shop shiitake and source naturals 16 mushroom mix affect nitric oxide? [Re: Juke Adro]
#9085198 - 10/16/08 03:44 AM (3 years, 7 months ago) |
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Thanks alot. I was thinking about contacting him anyway as I noticed his research.
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Juke Adro
I love peach fluff
Registered: 04/05/08
Posts: 5,768
Loc: Inside your head
Last seen: 1 year, 8 months
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Re: How does shop shiitake and source naturals 16 mushroom mix affect nitric oxide? [Re: nitricoxide]
#9085211 - 10/16/08 03:57 AM (3 years, 7 months ago) |
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Someone said: im actually not using ms, im using prints.
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nitricoxide
Stranger
Registered: 10/15/08
Posts: 3
Last seen: 3 years, 6 months
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Re: How does shop shiitake and source naturals 16 mushroom mix affect nitric oxide? [Re: Juke Adro]
#9099723 - 10/19/08 11:00 AM (3 years, 7 months ago) |
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He still hasn't replied by email. Damn! He must be busy.
Actually he says his access to technology is limited.
Edited by nitricoxide (10/19/08 11:05 AM)
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Juke Adro
I love peach fluff
Registered: 04/05/08
Posts: 5,768
Loc: Inside your head
Last seen: 1 year, 8 months
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Re: How does shop shiitake and source naturals 16 mushroom mix affect nitric oxide? [Re: nitricoxide]
#9099732 - 10/19/08 11:02 AM (3 years, 7 months ago) |
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He will, he replied to me within about 3 days with my last question.
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Someone said: im actually not using ms, im using prints.
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