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 imnotthere
Registered: 02/23/08
Posts: 343
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 hericium LC expanding. and thank you works! few questions about hericium culture
#9013769 - 10/01/08 01:48 PM (3 years, 7 months ago) |
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ordered agar kit from sporeworks, mentioned the forums, and no shit got a free hericium culture!!! I've been wanting to start some of these: its like they read my mind!
anyways, is the culture just an LC basically?
Like could I put a drop of the sporeworks LC into my own sterile honey LC solution and expand this culture? I even have a magnetic stirrer. And could this kind of LC be maintained for long term storage and use?
Should I keep this syringe with culture in the fridge?
I'd like to expand and make my own LC, to conserve the original contents, so i don't have to clone or print my lion manes.
I'm gonna grow on rye. and spawned to sawdust in bags... so could I just inoculate a 1/2 pint jar with 1 drop of hericium culture, or with my LC, then g2g to get many many quarts? like start with 1/2 pint and g2g to like 10-15 quarts for example....
I assume g2g is easily accomplishe with hericium.
most of the above questions is an attempt to conserve my culture, cause I understand lions mane are very difficult to spore print or to clone. and I am new to them anyways. so suggestions on the above would be much appreciated!
How does the mycelium degenerate as well? same as for other species? like keep the g2g generations to like 2 instead of 3 and 4....
can agar be done with my hericium culture syringe? I am gonna learn agar anyways, would this be beneficial to agar and sector hericium mycelium??? do people do agar with hericium as I have searched but found nothing. would it be possible to agar and sector until getting good rhizo growth and mycelium that fruits nicely on agar, before attempting a grow??? I'm only using what I know about other species to understand, so please someone with knowledge set me straight,
thank you all!
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lipa
Registered: 07/24/07
Posts: 1,688
Loc: USA 
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 Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: imnotthere]
#9014191 - 10/01/08 03:16 PM (3 years, 7 months ago) |
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Please do some searching before you post a million questions. Almost all of these questions can be answered by the search.Search Holy crap!
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 Paresthesia
Stranger
Registered: 07/02/08
Posts: 1,051
Loc: Texas
Last seen: 3 months, 4 days
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 Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: lipa]
#9014925 - 10/01/08 05:30 PM (3 years, 7 months ago) |
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Quote:
lipa said:
Please do some searching before you post a million questions. Almost all of these questions can be answered by the search.Search Holy crap!
Daaaaaamn!
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"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."
- T. S. Eliot
I'm currently looking for cultures of the following species:
Calocybe indica, Chlorophyllum rachodes, Lentinula boryana, Polyporus umbellatus
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Paresthesia
Stranger
Registered: 07/02/08
Posts: 1,051
Loc: Texas
Last seen: 3 months, 4 days
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Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: imnotthere]
#9015003 - 10/01/08 05:46 PM (3 years, 7 months ago) |
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Yes, the Sporeworks is the awesome. It's nice not to have to shell out $200 for a good culture. 
Without getting into detail, most techniques on this site for LC and agar cultures will work with non-actives. The Lion's Mane culture you received from Sporeworks is already an isolated strain, so transferring it to agar isn't necessary. You can use it to incolulate grain directly or expand it by using it to seed more LC.
Lipa is correct in stating that most of these questions can be answered with a few searches. Another really great source of information for you would be Paul Stamets' "Growing Gourmet and Medicinal Mushrooms." I held off on buying it for a few weeks when I first got started, and it would have helped me avoid a lot of dumb mistakes!
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"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."
- T. S. Eliot
I'm currently looking for cultures of the following species:
Calocybe indica, Chlorophyllum rachodes, Lentinula boryana, Polyporus umbellatus
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imnotthere
Registered: 02/23/08
Posts: 343
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Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: Paresthesia]
#9015378 - 10/01/08 06:56 PM (3 years, 7 months ago) |
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oh what, search function, huh, who?
come on dudes, i don't need that crap i know and search and read for hours on this shit before posting.
I was merely asking about some terminology, and if some of these things were possible - NOT HOW TO DO THEM necessarily.
Quote:
Paresthesia said:
Without getting into detail, most techniques on this site for LC and agar cultures will work with non-actives. The Lion's Mane culture you received from Sporeworks is already an isolated strain, so transferring it to agar isn't necessary. You can use it to incolulate grain directly or expand it by using it to seed more LC.
that was an excellent help, and not something I easily found with the search. Often times not fully understanding the terminology henders your efforts in the search. Doesn't always mean you didn't try, shit.
I mostly wanted to know that it is already an isolate, and agar isn't necessary. Not easily searched for.
Now I admit a few of my questions were no brainers, they kinda came out as I was seeking my main question. Mostly I was just hoping someone with experience would enlighten me on those terminology and agar questions, not mock my questions. Now I recall why I do the hours of reading I do with very few posts, to avoid that kind of slack.
I asked only four questions, of varying difficulty. I just get a little wordy when I explain myself.
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b3jamboree
yes we have no portabellas
Registered: 09/11/06
Posts: 423
Loc: 45th Parallel, MI
Last seen: 5 months, 24 days
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Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: imnotthere]
#9017328 - 10/02/08 07:12 AM (3 years, 7 months ago) |
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Lion's Mane is not an ideal strain for G2G. It will work, but using LC is much better. It is a slow growing strain and often the mycelium will colonize very unevenly and contaminate in certain areas.
That being said sporeworks has a good strain and I have had some good success with it. I wish you the best of luck, lion's manes are pretty tricky.
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imnotthere
Registered: 02/23/08
Posts: 343
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Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: b3jamboree]
#9017488 - 10/02/08 08:14 AM (3 years, 7 months ago) |
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thanks b3jamboree, that is something I didn't know. I guess I'd be better off using just a tad of that sporeworks culture to make a larger LC. Is a 4% honey solution good for a Lion's Mane LC?
I wonder what sporeworks uses for their culture syringe, I'm sure it makes no difference though and I can use what ever medium I choose. any advice?
good to see there are helpful folks here who don't jump to conclusions and flame for asking questions. If anything my low post count shows I read a shit ton, and thats that.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 36,974
Loc: USA Mountain Northwest
Last seen: 18 hours, 57 minutes
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Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: b3jamboree]
#9017496 - 10/02/08 08:17 AM (3 years, 7 months ago) |
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I'm pretty sure I sent workman a culture of my hericium strain, but have no idea if that's the one they're sending out or not. If so, it's a very aggressive strain and will perform well. In fact it will begin pinning on rye grains at about the time of full colonization, which only takes seven to ten days or so. It works fine for grain to grain transfers.
If you have the culture in a syringe, you can squirt a drop of solution on agar if you want to check for or isolate away from any contaminants that may be present.
Hericium fruits well on a wide range of substrates, but in my experience sawdust/woodchip blocks supplemented with wheat, rice, or oat bran will give the best and largest fruits. Use up to 10% gypsum in the blocks. I've also had some great fruitings with old phonebooks and newspapers.
RR
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www.mushroomvideos.com
semper in excretia sumus solim profundum variat
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lipa
Registered: 07/24/07
Posts: 1,688
Loc: USA
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Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: RogerRabbit]
#9017870 - 10/02/08 10:16 AM (3 years, 7 months ago) |
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Quote:
If you have the culture in a syringe, you can squirt a drop of solution on agar if you want to check for or isolate away from any contaminants that may be present
I think you would be better off doing this anyways. It is a good idea. In my short experience with this species high inoculation rates are a good thing. I would expand it into a larger amount of LC before venturing into the spawn. I also agree grain to grain is just poor. I did it, but the second generation was just slow as a snail and too quick to fruit. Did not like shaking at all! Make sure you keep your grain and blocks in complete darkness during colonization. When you get to fruiting make sure you have a healthy supply of fresh air. I agree with RR on the gypsum 10% is good. Medium sized crushed oyster shells worked very well for me also. I am also working with hericium right now and it is very satisfying in the end. Also I have noticed(although this could be strain specific) that fruits form nicer when the holes are punched just at or right below the top of the substrate for fruiting.
I apologize if you took my post offensively. I didn't mean it to be. It's best to ask just a few questions at a time through the course of a thread as you discuss things. It's easier for the responders to answer them and better for you to help you remember through discussion.
Good luck and happy growing
LIPA
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imnotthere
Registered: 02/23/08
Posts: 343
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Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: lipa]
#9018235 - 10/02/08 12:00 PM (3 years, 7 months ago) |
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thanks for the advice
Quote:
RogerRabbit said:
Hericium fruits well on a wide range of substrates, but in my experience sawdust/woodchip blocks supplemented with wheat, rice, or oat bran will give the best and largest fruits. Use up to 10% gypsum in the blocks.
RR
what do you mean by blocks? Just like bags standing upright? I plan on doing just like what you said here once I find a local source for hardwood sawdust. I assume the brans can be had at health food stores. Which is better, sawdust or woodchips, or a combination of both? Seems like fine sawdust would make for a very dense substrate, too dense.
Do my bags I plan to fruit in need filter patches? I imagine large freezer kind of ziplocs would work. Leaving the top open for FAE, and cutting a few slits at and below sub level like LIPA suggested. I will doing the fruiting in a GH of course, I have great FAE in there and can't wait to get my bags in there.
Great picture LIPA, thats exactly what I am shooting for. What kind of bag is that?
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lipa
Registered: 07/24/07
Posts: 1,688
Loc: USA
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Re: hericium LC expanding. and thank you works! few questions about hericium culture [Re: imnotthere]
#9018291 - 10/02/08 12:15 PM (3 years, 7 months ago) |
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Don't use zip lock bags. They are more suited for plain pasteurized sawdust for mushrooms such as Trametes versicolor that don't need supplementation. You need to use a filter patch bag or a oven bag with a piece of cotton stuffed in the top. After spawning to the sawdust and colonization is complete there you will have your block. You then take a nail and punch about 2 to 4 holes near the top of the block through the bag to induce fruiting on the outside. You should punch the holes just before colonization' like the day before or you will get lots of fruiting within the bag. Some also just cut the top of the bag off and allow it to fruit off the top. I however would prefer to use the side for easy removal and less contamination. If you do not want to buy supplements look for a soy dairy nearby and ask for some soy waste. They usually put it in barrels and send it to cattle farms. The stuff is potent and usually free. It's best not to have to buy supplements because they are usually pretty expensive.
A combination of sawdust and chips is best. If the sawdust is too fine add some coir, perlite, or vermiculite to loosen it up. Don't over saturate or you block will become dead due to lack of air.
That is a filter patch bag. you can get them from sporeworks as well. They have 2 sizes.
LIPA
Edited by lipa (10/02/08 12:30 PM)
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